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The map label for this gene is emrA [H]

Identifier: 78222587

GI number: 78222587

Start: 1536773

End: 1537897

Strand: Reverse

Name: emrA [H]

Synonym: Gmet_1375

Alternate gene names: 78222587

Gene position: 1537897-1536773 (Counterclockwise)

Preceding gene: 78222588

Following gene: 78222586

Centisome position: 38.47

GC content: 64.89

Gene sequence:

>1125_bases
ATGGCAGTGGAAGAATCGAAGGAAAATCAAGGGGCGGGAGAAACCGCCAGAACCGCAACGGATACGGCCGGCCCAGCGGG
CAAGCAGCGGGGAGGGAAGCGGACGCGGGCAGGAATCATTCTCCTCGCGGTGATCGCCATCGGGCTGTTCATGGGAACGC
ACTGGTTCTTACGGAGCAAGACCCTCATCACCACCGACAACGCCTTTGTTGAGGCCCACATCCACGCGATATCGCCGCGG
GTTTCGGGCACCGTGACCGCTGTCCCGGTGATGGACAACCAGTTTGTGAAAAAGGGGGAGACCCTGGTGGAGCTGGACCC
TACGGACTATGAGGTTCGGGTCCGGTCGGCGGCCGCCGACCTTGACATGGCGCGCAATGAAACCTCGGGGGACTACGCCC
AGGTGGAAGCGGCCCGGGCAGCGGTTTCCCACGCCCGGGCGCGGCTGGACCAGGCGGAGCTGGACCTCAGGCGCGGAAGT
GTCCTCTTTACCAAGGAGGTGATACCGCGTGAGCAGCTGGATCGCCTCGAAACGGCACGACGGGTGACTGCTGCCCAGCT
TCGGGAGGCAGAGGAAGGGGTACGCAAGGCCCAGGCAGAGTTGGGTCTTGCCGGCACCGGCGGCAAAGAGGCCAGGGTGG
CCCAGCGGGAGGCGAAACTACGGGAAGCCTCCCTCAGCCTCTCCTACACGCGCATCGTTGCCCCCGTCGACGGCTACGTG
ACCCGCAAGTCCGTTGAGACGGGATCCACGGTCCAGGCAGGGCAGCCCCTCATGGCCGTGGTGGCCCTTAACGACACGTG
GGTGACGGCCAATTACAAGGAAAGCCAGCTTGCCCACATGAAACCGGGCCAGAAGGTGACGTTCACCGTGGACGCCTACC
CCTCCCGCACCTTTACCGGCCGGGTCGACAGCATCATGGCCGGCACCGGCGCCGCCTTCTCGCTCCTCCCGTCCGAGAAC
GCCACCGGCAACTACGTGAAGGTGGTGCAGCGAGTGCCGGTGAAGATCGTGGTGGATACGGGGAGCGACCCCCGGCATCT
GCTCCGGGTGGGAATGAGCGTGATCCCCACCGTGAACACCGGGCGCAAACTGGGGGAAATCCTCAGGGATTTCCGGCCCT
TCTAG

Upstream 100 bases:

>100_bases
AGACGCTCCTTACCCAGATCAGGACCGACTATTTCCGCGCCGTCTTCGACTACCAGGTGGCGGCGGCGCGGGTCAGAAAA
GCGATGGGAGAACTGTAGTC

Downstream 100 bases:

>100_bases
GACTGCGGGTTCATGGACGCACCGGTCAAGGACGTCAACAAGTGGATCGTCACCATCACCGTGATGCTTCCCACCATCAT
GGAGATCGTAGACACGTCGG

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MAVEESKENQGAGETARTATDTAGPAGKQRGGKRTRAGIILLAVIAIGLFMGTHWFLRSKTLITTDNAFVEAHIHAISPR
VSGTVTAVPVMDNQFVKKGETLVELDPTDYEVRVRSAAADLDMARNETSGDYAQVEAARAAVSHARARLDQAELDLRRGS
VLFTKEVIPREQLDRLETARRVTAAQLREAEEGVRKAQAELGLAGTGGKEARVAQREAKLREASLSLSYTRIVAPVDGYV
TRKSVETGSTVQAGQPLMAVVALNDTWVTANYKESQLAHMKPGQKVTFTVDAYPSRTFTGRVDSIMAGTGAAFSLLPSEN
ATGNYVKVVQRVPVKIVVDTGSDPRHLLRVGMSVIPTVNTGRKLGEILRDFRPF

Sequences:

>Translated_374_residues
MAVEESKENQGAGETARTATDTAGPAGKQRGGKRTRAGIILLAVIAIGLFMGTHWFLRSKTLITTDNAFVEAHIHAISPR
VSGTVTAVPVMDNQFVKKGETLVELDPTDYEVRVRSAAADLDMARNETSGDYAQVEAARAAVSHARARLDQAELDLRRGS
VLFTKEVIPREQLDRLETARRVTAAQLREAEEGVRKAQAELGLAGTGGKEARVAQREAKLREASLSLSYTRIVAPVDGYV
TRKSVETGSTVQAGQPLMAVVALNDTWVTANYKESQLAHMKPGQKVTFTVDAYPSRTFTGRVDSIMAGTGAAFSLLPSEN
ATGNYVKVVQRVPVKIVVDTGSDPRHLLRVGMSVIPTVNTGRKLGEILRDFRPF
>Mature_373_residues
AVEESKENQGAGETARTATDTAGPAGKQRGGKRTRAGIILLAVIAIGLFMGTHWFLRSKTLITTDNAFVEAHIHAISPRV
SGTVTAVPVMDNQFVKKGETLVELDPTDYEVRVRSAAADLDMARNETSGDYAQVEAARAAVSHARARLDQAELDLRRGSV
LFTKEVIPREQLDRLETARRVTAAQLREAEEGVRKAQAELGLAGTGGKEARVAQREAKLREASLSLSYTRIVAPVDGYVT
RKSVETGSTVQAGQPLMAVVALNDTWVTANYKESQLAHMKPGQKVTFTVDAYPSRTFTGRVDSIMAGTGAAFSLLPSENA
TGNYVKVVQRVPVKIVVDTGSDPRHLLRVGMSVIPTVNTGRKLGEILRDFRPF

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=355, Percent_Identity=36.056338028169, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1788711, Length=323, Percent_Identity=37.7708978328173, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1790519, Length=307, Percent_Identity=31.9218241042345, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1790024, Length=234, Percent_Identity=29.9145299145299, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1789637, Length=316, Percent_Identity=28.7974683544304, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790012, Length=234, Percent_Identity=26.9230769230769, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87081951, Length=307, Percent_Identity=25.4071661237785, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI87081796, Length=235, Percent_Identity=28.5106382978723, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787013, Length=272, Percent_Identity=26.8382352941176, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40440; Mature: 40308

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVEESKENQGAGETARTATDTAGPAGKQRGGKRTRAGIILLAVIAIGLFMGTHWFLRSK
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
TLITTDNAFVEAHIHAISPRVSGTVTAVPVMDNQFVKKGETLVELDPTDYEVRVRSAAAD
EEEEECCCEEEEEEEEECCCCCCEEEEEEECCCHHHHCCCEEEEECCCCEEEEEEHHHHH
LDMARNETSGDYAQVEAARAAVSHARARLDQAELDLRRGSVLFTKEVIPREQLDRLETAR
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHCCHHHHHHHHHHH
RVTAAQLREAEEGVRKAQAELGLAGTGGKEARVAQREAKLREASLSLSYTRIVAPVDGYV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEECCCCCE
TRKSVETGSTVQAGQPLMAVVALNDTWVTANYKESQLAHMKPGQKVTFTVDAYPSRTFTG
EECCCCCCCCCCCCCCEEEEEEECCEEEECCCCCCHHHHCCCCCEEEEEEECCCCCCCCC
RVDSIMAGTGAAFSLLPSENATGNYVKVVQRVPVKIVVDTGSDPRHLLRVGMSVIPTVNT
CHHHHHCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHCCCCCC
GRKLGEILRDFRPF
CHHHHHHHHHHCCC
>Mature Secondary Structure 
AVEESKENQGAGETARTATDTAGPAGKQRGGKRTRAGIILLAVIAIGLFMGTHWFLRSK
CCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
TLITTDNAFVEAHIHAISPRVSGTVTAVPVMDNQFVKKGETLVELDPTDYEVRVRSAAAD
EEEEECCCEEEEEEEEECCCCCCEEEEEEECCCHHHHCCCEEEEECCCCEEEEEEHHHHH
LDMARNETSGDYAQVEAARAAVSHARARLDQAELDLRRGSVLFTKEVIPREQLDRLETAR
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHCCHHHHHHHHHHH
RVTAAQLREAEEGVRKAQAELGLAGTGGKEARVAQREAKLREASLSLSYTRIVAPVDGYV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEECCCCCE
TRKSVETGSTVQAGQPLMAVVALNDTWVTANYKESQLAHMKPGQKVTFTVDAYPSRTFTG
EECCCCCCCCCCCCCCEEEEEEECCEEEECCCCCCHHHHCCCCCEEEEEEECCCCCCCCC
RVDSIMAGTGAAFSLLPSENATGNYVKVVQRVPVKIVVDTGSDPRHLLRVGMSVIPTVNT
CHHHHHCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHCCCCCC
GRKLGEILRDFRPF
CHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]