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The map label for this gene is trmJ [H]

Identifier: 78222581

GI number: 78222581

Start: 1532028

End: 1532774

Strand: Direct

Name: trmJ [H]

Synonym: Gmet_1369

Alternate gene names: 78222581

Gene position: 1532028-1532774 (Clockwise)

Preceding gene: 78222580

Following gene: 78222582

Centisome position: 38.33

GC content: 61.45

Gene sequence:

>747_bases
ATGACCGTTTCGATGAAGAATAATATTTCAGTCATCCTCGTGGAACCCCAGAGTCCGGGTAACGTGGGGATGGTCTGCCG
GGCCATGAAGAACATGGGTCTGGCTGAACTCCGCATCGTCAAGGGATGCCCCCTCGACCATCCCGAAGCATACAAGTTTG
CCGTTTCCGCCAAGGACCTCCTGGAGGGGGCGCGGGTCTTTCCATCCCTCGAAGAGGCCCTGGCCGACACGGAGCTCTCC
GTTGCCACCACCCGCCGTCACGGCAAGTACCGGCAGGAGATTTACTCTCCCGGGGAAATAGCAGGGAAGATCGTCACGAA
CCTGGCCGGCAACCGTGCGGCCCTCGTTTTTGGCCGGGAAGACAGCGGTCTGACCACCGACGAGCTTTCCCTCTGCCGGT
GGCACGCCACCATCCCCACCTCCGCAGAATATGGATCATTGAACCTGGCCCAGGCGGTGTTGATCTTCTGCTACGAACTT
TTTGCGGGATTGGGTGAGGGAACCGTTGCGGGGGAGGCCCGGACCCTGGCAGGCAGCGCTGCCCAGGAGGCCCTCTTCGG
CCAGATGGAGCGGACGCTCTTGCGGATCGGTTTCCTCAATCCCCAGAACCCTGACCACATCATGCGGACAGTGCGGCGCA
TCTTTTCTCGGGCCGAGCTGGATGACCGCGAGGTGGCCATCCTGCGCGGCATGATGACCCAGATCGATTGGGCCGCCGAC
CAGTTCAAGGGGAAGAAGGGGCAATGA

Upstream 100 bases:

>100_bases
TGCCTGGTTCCGGCGCAAGCCGGCCCGAACGAGGTTGAAGAGGATAAGGGATAGCCGAAAAACCGTGGATCAGTAACAAA
GAGATGTGCTGGAGACCTCG

Downstream 100 bases:

>100_bases
ACGGCGAGATAATGCTGGGGCTGGTGGCCGGCGCGGTGACGAGCATCGCGGTGGTGCCGCAGGTGGCCCGGGCCTACCGT
ACCAAGCGTGTGCGGGACAT

Product: RNA methyltransferase TrmH, group 1

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MTVSMKNNISVILVEPQSPGNVGMVCRAMKNMGLAELRIVKGCPLDHPEAYKFAVSAKDLLEGARVFPSLEEALADTELS
VATTRRHGKYRQEIYSPGEIAGKIVTNLAGNRAALVFGREDSGLTTDELSLCRWHATIPTSAEYGSLNLAQAVLIFCYEL
FAGLGEGTVAGEARTLAGSAAQEALFGQMERTLLRIGFLNPQNPDHIMRTVRRIFSRAELDDREVAILRGMMTQIDWAAD
QFKGKKGQ

Sequences:

>Translated_248_residues
MTVSMKNNISVILVEPQSPGNVGMVCRAMKNMGLAELRIVKGCPLDHPEAYKFAVSAKDLLEGARVFPSLEEALADTELS
VATTRRHGKYRQEIYSPGEIAGKIVTNLAGNRAALVFGREDSGLTTDELSLCRWHATIPTSAEYGSLNLAQAVLIFCYEL
FAGLGEGTVAGEARTLAGSAAQEALFGQMERTLLRIGFLNPQNPDHIMRTVRRIFSRAELDDREVAILRGMMTQIDWAAD
QFKGKKGQ
>Mature_247_residues
TVSMKNNISVILVEPQSPGNVGMVCRAMKNMGLAELRIVKGCPLDHPEAYKFAVSAKDLLEGARVFPSLEEALADTELSV
ATTRRHGKYRQEIYSPGEIAGKIVTNLAGNRAALVFGREDSGLTTDELSLCRWHATIPTSAEYGSLNLAQAVLIFCYELF
AGLGEGTVAGEARTLAGSAAQEALFGQMERTLLRIGFLNPQNPDHIMRTVRRIFSRAELDDREVAILRGMMTQIDWAADQ
FKGKKGQ

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=252, Percent_Identity=38.8888888888889, Blast_Score=148, Evalue=3e-37,
Organism=Escherichia coli, GI1790865, Length=153, Percent_Identity=41.1764705882353, Blast_Score=116, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27118; Mature: 26986

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSMKNNISVILVEPQSPGNVGMVCRAMKNMGLAELRIVKGCPLDHPEAYKFAVSAKDL
CEEEECCCEEEEEECCCCCCCHHHHHHHHHHCCHHHEEEECCCCCCCCCHHEEEECHHHH
LEGARVFPSLEEALADTELSVATTRRHGKYRQEIYSPGEIAGKIVTNLAGNRAALVFGRE
HHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEECC
DSGLTTDELSLCRWHATIPTSAEYGSLNLAQAVLIFCYELFAGLGEGTVAGEARTLAGSA
CCCCCHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
AQEALFGQMERTLLRIGFLNPQNPDHIMRTVRRIFSRAELDDREVAILRGMMTQIDWAAD
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
QFKGKKGQ
HCCCCCCC
>Mature Secondary Structure 
TVSMKNNISVILVEPQSPGNVGMVCRAMKNMGLAELRIVKGCPLDHPEAYKFAVSAKDL
EEEECCCEEEEEECCCCCCCHHHHHHHHHHCCHHHEEEECCCCCCCCCHHEEEECHHHH
LEGARVFPSLEEALADTELSVATTRRHGKYRQEIYSPGEIAGKIVTNLAGNRAALVFGRE
HHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEECC
DSGLTTDELSLCRWHATIPTSAEYGSLNLAQAVLIFCYELFAGLGEGTVAGEARTLAGSA
CCCCCHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
AQEALFGQMERTLLRIGFLNPQNPDHIMRTVRRIFSRAELDDREVAILRGMMTQIDWAAD
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
QFKGKKGQ
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA