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The map label for this gene is proS

Identifier: 78222566

GI number: 78222566

Start: 1515493

End: 1517205

Strand: Direct

Name: proS

Synonym: Gmet_1354

Alternate gene names: 78222566

Gene position: 1515493-1517205 (Clockwise)

Preceding gene: 78222565

Following gene: 78222567

Centisome position: 37.91

GC content: 61.59

Gene sequence:

>1713_bases
ATGCGCTATTCCCAGTATTTCATCCCCACGGTCAAGGAGACTCCTTCCGACGCCGAGGTCGTTTCCCACAAGCTCATGCT
CCGTGCTGGCATGATCCGCAAGCTCGCCGCCGGCATCTACAACTATCTTCCCCTGGGGCTCCGCTCCATCCGCAAGGTCG
AACAGATTGTCCGCGAGGAGATGAACCGGGCCGGTGCCATCGAGCTTCTCATGCCGAGCGTCCAGCCGGCTGAGCTCTGG
CAGGAGTCGAAGCGGTGGGAGCAGTACGGCAAAGAACTTCTCCGCTTCAAGGATCGCAAGGACGCCGAATTTTGCCTTGG
ACCGACCCACGAGGAGGTCATTACCGACCTCGTGCGCCGCGAGGTGAAGAGCTACCGGCAGCTGCCCTTGAATCTCTATC
AGGTACAATCCAAGTTCCGGGACGAGATCCGTCCCCGGTTCGGTCTCATGCGGGGCCGCGAGTTCATTATGAAGGATGCC
TATTCCTTTGACGTGAGCTCCGAGGCGGCTGATACCTCCTATGACAAGATGTATCAGGCCTACCGCCGCATCTTCCAGCG
CTGCGGCCTCAAGTTCCGGGCAGTGGAGGCGGATACCGGCTCCATCGGCGGCTCCTCGTCCCACGAGTTCATGGTGCTTG
CCGATTCGGGTGAGGATGCCATCGTCTCCTGCACCGCCTGCGAGTATGCCGCCAACGTGGAGAAGGCCGAGGCCCGGCTC
TTCCCGTCCGAGCATGCCGAGCCCCGGGAACTGGAAAAAGTGGAGACCCCCCAGAAGCGGAGCGTCGAAGAGGTGACCAC
CTTCCTCGGCATTCCCGCCTCGTCTCTGGTGAAGACCCTCCTCTGCGTTGCTGACGGAGAGCCGGTGGCAGCGTTGGTGC
GTGGCGACCACGACCTGAACGAGATCAAGCTCAAGCACCTTCTCGGCTGCGAAGAGTTGGAGATGGCCAGTGAGGAGATC
GTGGAGCGGGTCACCGGCGCTCCCGTCGGCTTTGCCGGCCCCGTGGGGTTGAAGATCAAGATCGTCGCCGACTTGACGAT
CCAGGGAATGAAAAACTTTGTCACCGGCGGCAATGCCCGTGATCTCCATTTCAAGAACGTGAACATCGGTAGGGATTTTA
CCCCTGCGCTCATTGCCGACATCCGCAACGTGGTCCACGGCGACCCCTGTCCCCGCTGCGAGGCCGGGCACCTGGAGATG
TGGCGCGGCATCGAGGTCGGGCACGTCTTCAAGCTGGGAACCAAGTACTCCGAGTCCCTCCGGGCGACCTTTCTGGACGC
CGACGGCAAGGAGCAGGTCATCTTCATGGGATGCTACGGCATCGGCATCAGTCGGACCGTGGCCGCCTGCATCGAGCAGA
ACCACGACGCCGACGGCATCATCTTCCCGATCCCCATCGCGCCGTTCCACTGCATCATCTCGGCCGTCAGCACGAAGGAT
GCGGAGGTCGTGGCCGCGTGCGATGAACTCTATCGTGCCCTCACTGCTGTCGGCGTCGAGGTTCTCTTTGACGACCGCGA
CGAGCGCCCCGGTTCCAAGTTCAAGGACGCCGACCTTATCGGCATCCCGCTCAGGATAGTCGTGGGGAGCAAGAACCTGG
CGGAAGGTAAGGTTGAACTCAAATCCCGCAAGGGCGGTGAGGTTTCTCTCCTCCCCCTGGCCGAGGCAGTGGAGACGGTC
AAGGGGCTCGTCGTTGCAGCTCTCAATCAGTAA

Upstream 100 bases:

>100_bases
AGATCGTCCGCAAGGTCCCCGAAGCCGAGATGGCCGATGCCTTGATCGCCGAGGTGGAGCAAATGGCCAAGGAAAAAACA
CACTCATAGAGGTTCGTTTA

Downstream 100 bases:

>100_bases
TATCCATTCAAGGAGACGATACGCACAATGACGAGAGACCAGGCCCGCGAAAAGGTCATTTTTGCCCTTGATACCGGTGA
ATTCGCCCATGTGCAGTACT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MRYSQYFIPTVKETPSDAEVVSHKLMLRAGMIRKLAAGIYNYLPLGLRSIRKVEQIVREEMNRAGAIELLMPSVQPAELW
QESKRWEQYGKELLRFKDRKDAEFCLGPTHEEVITDLVRREVKSYRQLPLNLYQVQSKFRDEIRPRFGLMRGREFIMKDA
YSFDVSSEAADTSYDKMYQAYRRIFQRCGLKFRAVEADTGSIGGSSSHEFMVLADSGEDAIVSCTACEYAANVEKAEARL
FPSEHAEPRELEKVETPQKRSVEEVTTFLGIPASSLVKTLLCVADGEPVAALVRGDHDLNEIKLKHLLGCEELEMASEEI
VERVTGAPVGFAGPVGLKIKIVADLTIQGMKNFVTGGNARDLHFKNVNIGRDFTPALIADIRNVVHGDPCPRCEAGHLEM
WRGIEVGHVFKLGTKYSESLRATFLDADGKEQVIFMGCYGIGISRTVAACIEQNHDADGIIFPIPIAPFHCIISAVSTKD
AEVVAACDELYRALTAVGVEVLFDDRDERPGSKFKDADLIGIPLRIVVGSKNLAEGKVELKSRKGGEVSLLPLAEAVETV
KGLVVAALNQ

Sequences:

>Translated_570_residues
MRYSQYFIPTVKETPSDAEVVSHKLMLRAGMIRKLAAGIYNYLPLGLRSIRKVEQIVREEMNRAGAIELLMPSVQPAELW
QESKRWEQYGKELLRFKDRKDAEFCLGPTHEEVITDLVRREVKSYRQLPLNLYQVQSKFRDEIRPRFGLMRGREFIMKDA
YSFDVSSEAADTSYDKMYQAYRRIFQRCGLKFRAVEADTGSIGGSSSHEFMVLADSGEDAIVSCTACEYAANVEKAEARL
FPSEHAEPRELEKVETPQKRSVEEVTTFLGIPASSLVKTLLCVADGEPVAALVRGDHDLNEIKLKHLLGCEELEMASEEI
VERVTGAPVGFAGPVGLKIKIVADLTIQGMKNFVTGGNARDLHFKNVNIGRDFTPALIADIRNVVHGDPCPRCEAGHLEM
WRGIEVGHVFKLGTKYSESLRATFLDADGKEQVIFMGCYGIGISRTVAACIEQNHDADGIIFPIPIAPFHCIISAVSTKD
AEVVAACDELYRALTAVGVEVLFDDRDERPGSKFKDADLIGIPLRIVVGSKNLAEGKVELKSRKGGEVSLLPLAEAVETV
KGLVVAALNQ
>Mature_570_residues
MRYSQYFIPTVKETPSDAEVVSHKLMLRAGMIRKLAAGIYNYLPLGLRSIRKVEQIVREEMNRAGAIELLMPSVQPAELW
QESKRWEQYGKELLRFKDRKDAEFCLGPTHEEVITDLVRREVKSYRQLPLNLYQVQSKFRDEIRPRFGLMRGREFIMKDA
YSFDVSSEAADTSYDKMYQAYRRIFQRCGLKFRAVEADTGSIGGSSSHEFMVLADSGEDAIVSCTACEYAANVEKAEARL
FPSEHAEPRELEKVETPQKRSVEEVTTFLGIPASSLVKTLLCVADGEPVAALVRGDHDLNEIKLKHLLGCEELEMASEEI
VERVTGAPVGFAGPVGLKIKIVADLTIQGMKNFVTGGNARDLHFKNVNIGRDFTPALIADIRNVVHGDPCPRCEAGHLEM
WRGIEVGHVFKLGTKYSESLRATFLDADGKEQVIFMGCYGIGISRTVAACIEQNHDADGIIFPIPIAPFHCIISAVSTKD
AEVVAACDELYRALTAVGVEVLFDDRDERPGSKFKDADLIGIPLRIVVGSKNLAEGKVELKSRKGGEVSLLPLAEAVETV
KGLVVAALNQ

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=226, Percent_Identity=48.6725663716814, Blast_Score=235, Evalue=1e-61,
Organism=Escherichia coli, GI1786392, Length=566, Percent_Identity=54.9469964664311, Blast_Score=610, Evalue=1e-176,
Organism=Caenorhabditis elegans, GI115532348, Length=215, Percent_Identity=40, Blast_Score=171, Evalue=9e-43,
Organism=Caenorhabditis elegans, GI193203271, Length=89, Percent_Identity=37.0786516853933, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320931, Length=557, Percent_Identity=30.8797127468582, Blast_Score=262, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24656200, Length=264, Percent_Identity=37.5, Blast_Score=185, Evalue=7e-47,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_GEOMG (Q39VY6)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384313.1
- ProteinModelPortal:   Q39VY6
- SMR:   Q39VY6
- STRING:   Q39VY6
- GeneID:   3738688
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1354
- NMPDR:   fig|269799.3.peg.1195
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- PhylomeDB:   Q39VY6
- ProtClustDB:   PRK09194
- BioCyc:   GMET269799:GMET_1354-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 63243; Mature: 63243

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYSQYFIPTVKETPSDAEVVSHKLMLRAGMIRKLAAGIYNYLPLGLRSIRKVEQIVREE
CCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNRAGAIELLMPSVQPAELWQESKRWEQYGKELLRFKDRKDAEFCLGPTHEEVITDLVRR
HHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHH
EVKSYRQLPLNLYQVQSKFRDEIRPRFGLMRGREFIMKDAYSFDVSSEAADTSYDKMYQA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
YRRIFQRCGLKFRAVEADTGSIGGSSSHEFMVLADSGEDAIVSCTACEYAANVEKAEARL
HHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCEEEEEHHHHHHCCHHHHHHC
FPSEHAEPRELEKVETPQKRSVEEVTTFLGIPASSLVKTLLCVADGEPVAALVRGDHDLN
CCCCCCCCHHHHHHCCCHHCCHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCHH
EIKLKHLLGCEELEMASEEIVERVTGAPVGFAGPVGLKIKIVADLTIQGMKNFVTGGNAR
HHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHCCCCCC
DLHFKNVNIGRDFTPALIADIRNVVHGDPCPRCEAGHLEMWRGIEVGHVFKLGTKYSESL
EEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCEEECCCHHHHHH
RATFLDADGKEQVIFMGCYGIGISRTVAACIEQNHDADGIIFPIPIAPFHCIISAVSTKD
HEEEECCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHCCCC
AEVVAACDELYRALTAVGVEVLFDDRDERPGSKFKDADLIGIPLRIVVGSKNLAEGKVEL
HHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCEECCCEEEEECCCCCCCCCEEE
KSRKGGEVSLLPLAEAVETVKGLVVAALNQ
ECCCCCCEEEEEHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRYSQYFIPTVKETPSDAEVVSHKLMLRAGMIRKLAAGIYNYLPLGLRSIRKVEQIVREE
CCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNRAGAIELLMPSVQPAELWQESKRWEQYGKELLRFKDRKDAEFCLGPTHEEVITDLVRR
HHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHH
EVKSYRQLPLNLYQVQSKFRDEIRPRFGLMRGREFIMKDAYSFDVSSEAADTSYDKMYQA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
YRRIFQRCGLKFRAVEADTGSIGGSSSHEFMVLADSGEDAIVSCTACEYAANVEKAEARL
HHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCEEEEEHHHHHHCCHHHHHHC
FPSEHAEPRELEKVETPQKRSVEEVTTFLGIPASSLVKTLLCVADGEPVAALVRGDHDLN
CCCCCCCCHHHHHHCCCHHCCHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCHH
EIKLKHLLGCEELEMASEEIVERVTGAPVGFAGPVGLKIKIVADLTIQGMKNFVTGGNAR
HHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHCCCCCC
DLHFKNVNIGRDFTPALIADIRNVVHGDPCPRCEAGHLEMWRGIEVGHVFKLGTKYSESL
EEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCEEECCCHHHHHH
RATFLDADGKEQVIFMGCYGIGISRTVAACIEQNHDADGIIFPIPIAPFHCIISAVSTKD
HEEEECCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHCCCC
AEVVAACDELYRALTAVGVEVLFDDRDERPGSKFKDADLIGIPLRIVVGSKNLAEGKVEL
HHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCEECCCEEEEECCCCCCCCCEEE
KSRKGGEVSLLPLAEAVETVKGLVVAALNQ
ECCCCCCEEEEEHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA