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The map label for this gene is pyrF

Identifier: 78222567

GI number: 78222567

Start: 1517233

End: 1517952

Strand: Direct

Name: pyrF

Synonym: Gmet_1355

Alternate gene names: 78222567

Gene position: 1517233-1517952 (Clockwise)

Preceding gene: 78222566

Following gene: 78222568

Centisome position: 37.96

GC content: 60.42

Gene sequence:

>720_bases
ATGACGAGAGACCAGGCCCGCGAAAAGGTCATTTTTGCCCTTGATACCGGTGAATTCGCCCATGTGCAGTACTGGGCCGA
AACGCTGTCCGACAAGGTCGGGATGTATAAAATCGGCAAGCAACTCTTCACCGCCTGCGGCCCAGCCGCGGTGCGAATGA
TCCAGAAGTTCGGCGGCGAGGTCTTTCTCGACCTCAAGTTCCACGACATCCCCAATACCGTTGCCATGGCCTCGGTGGAG
GCGGCCCGCATGGGAGTTAAACTTTTCAATCTCCATGCCCTGGGTGGCTATGAGATGATGGCGAAAACAGTGGAGGCCCT
CGACAAGGAATTCAAGGGAGGGGATAGGGCAAAGGTCCTGGCGGTCACTATTCTCACCTCTTCCACCGAGGAGACCCTCA
AGGATTTGGGGATTGAACACACCGTCCCCGACATGGTGGTGCGGCTCGCGACCCTTGCCAGGAAAGCCGGCATCGATGGC
GTCGTGGCTTCGCCCCGGGAGATTCCCCTCATCCGCGAAGCATGCGGGAGCGACTTCCTCATTGTCACTCCTGGCGTTCG
CCCGTCCTTCGCGGCCCTCAATGATCAGAAGCGGGTCATGACGCCGGCGGAGGCGGTGAAGGCGGGGAGTGATTATCTCG
TCATCGGCCGCCCCATTGGTGATGCGCCGGACCCGGCCGCTGCCGCCGAGCTGATTCTCGGCGAGATTGTTGCCGGCTGA

Upstream 100 bases:

>100_bases
GGTTTCTCTCCTCCCCCTGGCCGAGGCAGTGGAGACGGTCAAGGGGCTCGTCGTTGCAGCTCTCAATCAGTAATATCCAT
TCAAGGAGACGATACGCACA

Downstream 100 bases:

>100_bases
GCCGGATGAAAGGCTACGAGTGAAAGAAGAGATACTTTACGGGATCAACAGTGCCATGGAGGCCCTGCGCGGCAAGCGGA
GGGCCTTTGAGCTTTTCGTC

Product: orotidine-5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MTRDQAREKVIFALDTGEFAHVQYWAETLSDKVGMYKIGKQLFTACGPAAVRMIQKFGGEVFLDLKFHDIPNTVAMASVE
AARMGVKLFNLHALGGYEMMAKTVEALDKEFKGGDRAKVLAVTILTSSTEETLKDLGIEHTVPDMVVRLATLARKAGIDG
VVASPREIPLIREACGSDFLIVTPGVRPSFAALNDQKRVMTPAEAVKAGSDYLVIGRPIGDAPDPAAAAELILGEIVAG

Sequences:

>Translated_239_residues
MTRDQAREKVIFALDTGEFAHVQYWAETLSDKVGMYKIGKQLFTACGPAAVRMIQKFGGEVFLDLKFHDIPNTVAMASVE
AARMGVKLFNLHALGGYEMMAKTVEALDKEFKGGDRAKVLAVTILTSSTEETLKDLGIEHTVPDMVVRLATLARKAGIDG
VVASPREIPLIREACGSDFLIVTPGVRPSFAALNDQKRVMTPAEAVKAGSDYLVIGRPIGDAPDPAAAAELILGEIVAG
>Mature_238_residues
TRDQAREKVIFALDTGEFAHVQYWAETLSDKVGMYKIGKQLFTACGPAAVRMIQKFGGEVFLDLKFHDIPNTVAMASVEA
ARMGVKLFNLHALGGYEMMAKTVEALDKEFKGGDRAKVLAVTILTSSTEETLKDLGIEHTVPDMVVRLATLARKAGIDGV
VASPREIPLIREACGSDFLIVTPGVRPSFAALNDQKRVMTPAEAVKAGSDYLVIGRPIGDAPDPAAAAELILGEIVAG

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=231, Percent_Identity=42.4242424242424, Blast_Score=168, Evalue=3e-43,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_GEOMG (Q39VY5)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384314.1
- ProteinModelPortal:   Q39VY5
- STRING:   Q39VY5
- GeneID:   3738689
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1355
- NMPDR:   fig|269799.3.peg.1196
- eggNOG:   COG0284
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- PhylomeDB:   Q39VY5
- ProtClustDB:   CLSK828364
- BioCyc:   GMET269799:GMET_1355-MONOMER
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 25631; Mature: 25500

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 66-66 BINDING 15-15 BINDING 37-37 BINDING 126-126 BINDING 187-187 BINDING 196-196 BINDING 216-216 BINDING 217-217

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRDQAREKVIFALDTGEFAHVQYWAETLSDKVGMYKIGKQLFTACGPAAVRMIQKFGGE
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHCCCE
VFLDLKFHDIPNTVAMASVEAARMGVKLFNLHALGGYEMMAKTVEALDKEFKGGDRAKVL
EEEEEEECCCCCHHHHHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEE
AVTILTSSTEETLKDLGIEHTVPDMVVRLATLARKAGIDGVVASPREIPLIREACGSDFL
EEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCEE
IVTPGVRPSFAALNDQKRVMTPAEAVKAGSDYLVIGRPIGDAPDPAAAAELILGEIVAG
EECCCCCCCHHHCCCCCHHCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRDQAREKVIFALDTGEFAHVQYWAETLSDKVGMYKIGKQLFTACGPAAVRMIQKFGGE
CCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHCCCE
VFLDLKFHDIPNTVAMASVEAARMGVKLFNLHALGGYEMMAKTVEALDKEFKGGDRAKVL
EEEEEEECCCCCHHHHHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEE
AVTILTSSTEETLKDLGIEHTVPDMVVRLATLARKAGIDGVVASPREIPLIREACGSDFL
EEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCEE
IVTPGVRPSFAALNDQKRVMTPAEAVKAGSDYLVIGRPIGDAPDPAAAAELILGEIVAG
EECCCCCCCHHHCCCCCHHCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA