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The map label for this gene is kdsA

Identifier: 78222491

GI number: 78222491

Start: 1438750

End: 1439568

Strand: Direct

Name: kdsA

Synonym: Gmet_1277

Alternate gene names: 78222491

Gene position: 1438750-1439568 (Clockwise)

Preceding gene: 78222490

Following gene: 78222492

Centisome position: 35.99

GC content: 60.32

Gene sequence:

>819_bases
ATGACCCGAGAAATCGCCATTGGCAACGTGAAGATGGGGGGCAGCCGTCCCCTGGTGCTCATCGCCGGTCCCTGCGTTAT
TGAAAACGAGACGGCGACGCTCCGCTGCGCTGAACGCCTTATGACCATCGTGAACGGGTTGTCGATCCCGCTCATTTTCA
AGGCTTCCTATGACAAGGCGAACCGCACCTCGGTCACCGCCTTTCGGGGACCGGGGCTTAAAGAGGGGCTGCGCATCCTC
GCCAAGGTCAAGGAGTCTCTGGGCCTTCCGGTTATCTCCGACATCCACTCCATCGAGCAGGTGCAGCCTGCGGCAGAGGT
TCTCGACATTCTTCAGATTCCGGCGTTTCTCTGCCGCCAGACCGATCTCCTGGTGGAAGCAGCCCGTACGGGATGCGTGG
TGAACGTGAAGAAGGGGCAGTTTCTTGCCCCGTGGGACATGGAGAATGTGGTCGGCAAGATCGTTGCCAGTGGGAACGAG
CGGATCATCCTCACGGAGCGCGGTGCCTCCTTCGGCTACAACAACCTGGTGTCCGACATGCGGAGCCTCCCCATCATGCG
GCGGTTCGGTTTTCCGGTGGTTTTCGACGCAACCCACAGCGTCCAGCTCCCGGGGGGGCAGGGTGGTTCGTCCGGCGGAC
AGCGAGAGTTCGTGGAGTATCTTTCCCGTGCCGCGGTGGCAACGGGCATTGACGGGGTATTTCTGGAGGTTCACGAGGAG
CCGGACAAGGCCCTTTGCGACGGCCCCAATTCGGTCCCCCTTGACGACCTTCCCGTTCTGCTGAAAAAACTCAAGGCCAT
AGACGCCATAGTAAAATAG

Upstream 100 bases:

>100_bases
CAATCCCCATCCGCTCTTCAGGACGTTCATTGCCGCTGCCCTGGAACACCGGGGCAAGAGATCACAGGCTTAGTTCATCT
GTCTAGGTACCGAGGTTCCC

Downstream 100 bases:

>100_bases
GAGTCTGGAGCGGGCGCCCTGGAGCAGGTAGATGCATTTACCGGTTTCTGGTCCTCGGTCCTCGATCACGGATTTCAGAT
GATTATCGAAGAAGCACGCA

Product: 2-dehydro-3-deoxyphosphooctonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase; KDO-8-phosphate synthase; KDO 8-P synthase; KDOPS; Phospho-2-dehydro-3-deoxyoctonate aldolase

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MTREIAIGNVKMGGSRPLVLIAGPCVIENETATLRCAERLMTIVNGLSIPLIFKASYDKANRTSVTAFRGPGLKEGLRIL
AKVKESLGLPVISDIHSIEQVQPAAEVLDILQIPAFLCRQTDLLVEAARTGCVVNVKKGQFLAPWDMENVVGKIVASGNE
RIILTERGASFGYNNLVSDMRSLPIMRRFGFPVVFDATHSVQLPGGQGGSSGGQREFVEYLSRAAVATGIDGVFLEVHEE
PDKALCDGPNSVPLDDLPVLLKKLKAIDAIVK

Sequences:

>Translated_272_residues
MTREIAIGNVKMGGSRPLVLIAGPCVIENETATLRCAERLMTIVNGLSIPLIFKASYDKANRTSVTAFRGPGLKEGLRIL
AKVKESLGLPVISDIHSIEQVQPAAEVLDILQIPAFLCRQTDLLVEAARTGCVVNVKKGQFLAPWDMENVVGKIVASGNE
RIILTERGASFGYNNLVSDMRSLPIMRRFGFPVVFDATHSVQLPGGQGGSSGGQREFVEYLSRAAVATGIDGVFLEVHEE
PDKALCDGPNSVPLDDLPVLLKKLKAIDAIVK
>Mature_271_residues
TREIAIGNVKMGGSRPLVLIAGPCVIENETATLRCAERLMTIVNGLSIPLIFKASYDKANRTSVTAFRGPGLKEGLRILA
KVKESLGLPVISDIHSIEQVQPAAEVLDILQIPAFLCRQTDLLVEAARTGCVVNVKKGQFLAPWDMENVVGKIVASGNER
IILTERGASFGYNNLVSDMRSLPIMRRFGFPVVFDATHSVQLPGGQGGSSGGQREFVEYLSRAAVATGIDGVFLEVHEEP
DKALCDGPNSVPLDDLPVLLKKLKAIDAIVK

Specific function: Synthesis Of Kdo 8-P Which Is Required For Lipid A Maturation And Cellular Growth. [C]

COG id: COG2877

COG function: function code M; 3-deoxy-D-manno-octulosonic acid (KDO) 8-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsA family

Homologues:

Organism=Escherichia coli, GI1787466, Length=270, Percent_Identity=46.2962962962963, Blast_Score=271, Evalue=4e-74,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KDSA_GEOMG (Q39W61)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384238.1
- HSSP:   O66496
- ProteinModelPortal:   Q39W61
- SMR:   Q39W61
- STRING:   Q39W61
- GeneID:   3739138
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1277
- NMPDR:   fig|269799.3.peg.20
- eggNOG:   COG2877
- HOGENOM:   HBG575990
- OMA:   RSLPIMA
- PhylomeDB:   Q39W61
- ProtClustDB:   PRK05198
- BioCyc:   GMET269799:GMET_1277-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00056
- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006269
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21057:SF2
- TIGRFAMs:   TIGR01362

Pfam domain/function: PF00793 DAHP_synth_1

EC number: =2.5.1.55

Molecular weight: Translated: 29276; Mature: 29144

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTREIAIGNVKMGGSRPLVLIAGPCVIENETATLRCAERLMTIVNGLSIPLIFKASYDKA
CCCEEEECCEEECCCCCEEEEECCEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCC
NRTSVTAFRGPGLKEGLRILAKVKESLGLPVISDIHSIEQVQPAAEVLDILQIPAFLCRQ
CCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
TDLLVEAARTGCVVNVKKGQFLAPWDMENVVGKIVASGNERIILTERGASFGYNNLVSDM
HHHHHHHHHCCEEEEECCCCEECCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHH
RSLPIMRRFGFPVVFDATHSVQLPGGQGGSSGGQREFVEYLSRAAVATGIDGVFLEVHEE
HHCCHHHHCCCCEEEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECC
PDKALCDGPNSVPLDDLPVLLKKLKAIDAIVK
CCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TREIAIGNVKMGGSRPLVLIAGPCVIENETATLRCAERLMTIVNGLSIPLIFKASYDKA
CCEEEECCEEECCCCCEEEEECCEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCC
NRTSVTAFRGPGLKEGLRILAKVKESLGLPVISDIHSIEQVQPAAEVLDILQIPAFLCRQ
CCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
TDLLVEAARTGCVVNVKKGQFLAPWDMENVVGKIVASGNERIILTERGASFGYNNLVSDM
HHHHHHHHHCCEEEEECCCCEECCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHH
RSLPIMRRFGFPVVFDATHSVQLPGGQGGSSGGQREFVEYLSRAAVATGIDGVFLEVHEE
HHCCHHHHCCCCEEEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECC
PDKALCDGPNSVPLDDLPVLLKKLKAIDAIVK
CCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA