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The map label for this gene is kdsB

Identifier: 78222489

GI number: 78222489

Start: 1436258

End: 1437022

Strand: Direct

Name: kdsB

Synonym: Gmet_1275

Alternate gene names: 78222489

Gene position: 1436258-1437022 (Clockwise)

Preceding gene: 78222487

Following gene: 78222490

Centisome position: 35.93

GC content: 56.86

Gene sequence:

>765_bases
ATGAAAATAACAGCAATCATTCCTGCCAGATACGCCTCGACCCGTTTCCCCGGCAAGGCTCTCGCCGAGATCATTGGCAA
GCCAATGGTTCAGCATGTCTACGAGCGGACAGCCAAGGCCGGTCTGGTGTCCGAGGTTATTGTCGCTACCGATGACGAGC
GGGTTGCCGAAGCCGTTCGTGCTTTCGGAGGCCGGGTGGAAATGACTTCCGCGACCCACGAAACAGGTACCGACCGTCTT
GCCGAGGTTGCTGCCCGCATCGATGCAGATATCATTGTGAACGTTCAGGGTGATGAGCCCCTTATCGAACCTGCCATGAT
CGACGAGGCAATTGCGCCTCTGGTTGCCGACGCATCGATCCGGATGGGGACCCTCAAGAGCCGTATCAAGTCGCTGCACG
ATTTCCTGAGTCCCAATGTGGTTAAGGTCGTAACCGATCGCGAGGGAGATGCCCTGTACTTCTCCCGTTCGCCTCTCCCC
AACTTCCGCGACAAGTGGAACGACCTGAAGGACGAGGCGTTCGTCACCGGACGGCTTTTGTGTTACAAACACGTGGGGCT
CTATGTCTATCGACGCGACTTCCTCCTGGCGTTCGCCCGGATGGCGCCCACTCCCCTTGAGCTTGCGGAGAAACTGGAGC
AGTTGAGGGTTTTGGAGAACGGCTTCCGCATAAGGGTGGTGGAAACGTCCTTCGAGTCGATCGGTGTCGATACGCCGAGC
GATCTGGATAAGGTCGTCGAAAAGTTGTCGAGATTCCAAATCTGA

Upstream 100 bases:

>100_bases
TGTCGGTTGTCTTATTCAGCGCTCAGTGCCGGGCATCATCGATGCGTGGCGTTTATGCGTGCCGTCGTGTTTTGTGTGGG
GTGGAGCCGGAACTATTGTT

Downstream 100 bases:

>100_bases
AGGTTCGCGGGCACGAGGGGACGGTTAAAGCAGGCAATCCCCTTTCTGATGTCCCTTACCATTGGCTTGTTTTAGGAGCA
TTCATGAAAACCAAGTTTAT

Product: 3-deoxy-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase; CKS; CMP-KDO synthase

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MKITAIIPARYASTRFPGKALAEIIGKPMVQHVYERTAKAGLVSEVIVATDDERVAEAVRAFGGRVEMTSATHETGTDRL
AEVAARIDADIIVNVQGDEPLIEPAMIDEAIAPLVADASIRMGTLKSRIKSLHDFLSPNVVKVVTDREGDALYFSRSPLP
NFRDKWNDLKDEAFVTGRLLCYKHVGLYVYRRDFLLAFARMAPTPLELAEKLEQLRVLENGFRIRVVETSFESIGVDTPS
DLDKVVEKLSRFQI

Sequences:

>Translated_254_residues
MKITAIIPARYASTRFPGKALAEIIGKPMVQHVYERTAKAGLVSEVIVATDDERVAEAVRAFGGRVEMTSATHETGTDRL
AEVAARIDADIIVNVQGDEPLIEPAMIDEAIAPLVADASIRMGTLKSRIKSLHDFLSPNVVKVVTDREGDALYFSRSPLP
NFRDKWNDLKDEAFVTGRLLCYKHVGLYVYRRDFLLAFARMAPTPLELAEKLEQLRVLENGFRIRVVETSFESIGVDTPS
DLDKVVEKLSRFQI
>Mature_254_residues
MKITAIIPARYASTRFPGKALAEIIGKPMVQHVYERTAKAGLVSEVIVATDDERVAEAVRAFGGRVEMTSATHETGTDRL
AEVAARIDADIIVNVQGDEPLIEPAMIDEAIAPLVADASIRMGTLKSRIKSLHDFLSPNVVKVVTDREGDALYFSRSPLP
NFRDKWNDLKDEAFVTGRLLCYKHVGLYVYRRDFLLAFARMAPTPLELAEKLEQLRVLENGFRIRVVETSFESIGVDTPS
DLDKVVEKLSRFQI

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family

Homologues:

Organism=Escherichia coli, GI1787147, Length=249, Percent_Identity=48.1927710843374, Blast_Score=198, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KDSB_GEOMG (Q39W63)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384236.1
- ProteinModelPortal:   Q39W63
- SMR:   Q39W63
- STRING:   Q39W63
- GeneID:   3739774
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1275
- NMPDR:   fig|269799.3.peg.18
- eggNOG:   COG1212
- HOGENOM:   HBG637773
- OMA:   IIPARLK
- PhylomeDB:   Q39W63
- ProtClustDB:   PRK05450
- BioCyc:   GMET269799:GMET_1275-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00057
- InterPro:   IPR003329
- InterPro:   IPR004528
- TIGRFAMs:   TIGR00466

Pfam domain/function: PF02348 CTP_transf_3

EC number: =2.7.7.38

Molecular weight: Translated: 28303; Mature: 28303

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITAIIPARYASTRFPGKALAEIIGKPMVQHVYERTAKAGLVSEVIVATDDERVAEAVR
CEEEEEECCHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHH
AFGGRVEMTSATHETGTDRLAEVAARIDADIIVNVQGDEPLIEPAMIDEAIAPLVADASI
HCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCHH
RMGTLKSRIKSLHDFLSPNVVKVVTDREGDALYFSRSPLPNFRDKWNDLKDEAFVTGRLL
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCHHHHHCCHHHHHHHHHHHH
CYKHVGLYVYRRDFLLAFARMAPTPLELAEKLEQLRVLENGFRIRVVETSFESIGVDTPS
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCHHHCCCCCCH
DLDKVVEKLSRFQI
HHHHHHHHHHHCCC
>Mature Secondary Structure
MKITAIIPARYASTRFPGKALAEIIGKPMVQHVYERTAKAGLVSEVIVATDDERVAEAVR
CEEEEEECCHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHH
AFGGRVEMTSATHETGTDRLAEVAARIDADIIVNVQGDEPLIEPAMIDEAIAPLVADASI
HCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCHH
RMGTLKSRIKSLHDFLSPNVVKVVTDREGDALYFSRSPLPNFRDKWNDLKDEAFVTGRLL
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCHHHHHCCHHHHHHHHHHHH
CYKHVGLYVYRRDFLLAFARMAPTPLELAEKLEQLRVLENGFRIRVVETSFESIGVDTPS
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCHHHCCCCCCH
DLDKVVEKLSRFQI
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA