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The map label for this gene is gltA [H]

Identifier: 78222340

GI number: 78222340

Start: 1244476

End: 1245792

Strand: Direct

Name: gltA [H]

Synonym: Gmet_1124

Alternate gene names: 78222340

Gene position: 1244476-1245792 (Clockwise)

Preceding gene: 78222339

Following gene: 78222341

Centisome position: 31.13

GC content: 59.98

Gene sequence:

>1317_bases
ATGGGATTAAAAGAAACGCTTAAGCAGAAAATCGAGGAATTTCGCCCCCGCACCACCAGGCTTGTCAAAGAGTTCGGCAA
GGTCAAGATCGATGAGGTCACCATCGAGCAGTGTATCGGCGGCGCCCGCGACATTCGCAGTCTCGTGACCGATATCTCCT
ACCTGGACCCCCAGGAGGGAATCCGGTTCCGCGGCAAGACCATTCCCGAGACCTTTGCCGCCCTCCCCAAGGCGCCGGGT
TCTGATTACCCGACAGTCGAATCCTTCTGGTATTTCCTGCTGACGGGGGAGGTTCCCACCCAGGCCCAGGTGGATGAAGT
GCTGACCGAATGGAAGGTTCGCCAGGAAGTTCCTCAGTATGTTTTCGATACCATCCGGACCCTGCCCCGGGACAGCCATC
CCATGGCAATGCTCTCGGTGGGCATCACCGCCATGCAGCGGGACTCGAAGTTCGCCGCCCTCTACAATGCCGGCAAATTC
AACAAACTCTCTGCGTGGGAATCCGTGTACGAGGACGCCTGCGACATCGTCGCCCGCATTCCGGTCATCGCCGCATTCAT
CTATAACCTGAAGTACAAGGGCGACAAGCAGATCGCCATCGATCCCGCGCTGGACATGGGGGCAAATTTCGCCCACATGA
TCGACCAGGGCGATGCGTACAAGGATGTGGCACGGATGTACTTTATCCTCCACTCCGACCACGAATCCGGCAACGTCTCC
GCCCACACCACCCACCTGGTGCATTCCGCCCTGTCCGACCCCTACTATGCCTATGCCGCCGGGTTGAACGGCCTGGCCGG
TCCGCTCCACGGCCGCGCCAACCAGGAAGTGCTCGACTGGACCATCAAGTTCCAGGAGAAGTACTGCAAGGACCAGGAAC
CGACCAAGGAGCTCATCACCAGGGCGCTGTGGGATACCCTCAACTCCGGTCAGGTCATCCCCGGCTACGGCCATGCCGTC
CTGCGCAAAACCGATCCCCGCTACATTTCCCAGCGGGAGTTCTGCCAGAAGCACCTCCCGGAGGATCCCCTCTTCAAACT
GGTTTCCATGATCTTCGAGGTGGCTCCGGGCGTCCTCACCGAGCACGGCAAGACCAAGAATCCCTGGCCCAACGTCGATG
CCCAGTCCGGCGTCATCCAGTGGCACTACGGGGTCAAGGAATGGGATTTCTACACCGTTCTCTTCGGTGTCGGCCGGGCT
CTCGGCTGCATGGCAAACATCACCTGGGACCGGGGACTCGGCTATGCCATCGAGCGCCCGAAATCGGTAACCACCCCCAT
GCTCGAAGAGTGGGCCGCTAAAGGTGGCCGTCTGTAG

Upstream 100 bases:

>100_bases
TCATGGAATTGTTTCTTATCTGATCATCGTCGAAAGGAGGTTGTGAAGTTGTGTAGCGTTGGTTGATTTTAATGCAATTA
AGGAAAGGAGAGTTAGTACG

Downstream 100 bases:

>100_bases
TATCCGCGGTAAATGCGTAGCTTTACCGTATACATAGAAAACAAACAGGAGTTTACAATGGCAAAATACGGAACACTGCA
GGAGCGCGTGCGCTGCACGT

Product: type I citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MGLKETLKQKIEEFRPRTTRLVKEFGKVKIDEVTIEQCIGGARDIRSLVTDISYLDPQEGIRFRGKTIPETFAALPKAPG
SDYPTVESFWYFLLTGEVPTQAQVDEVLTEWKVRQEVPQYVFDTIRTLPRDSHPMAMLSVGITAMQRDSKFAALYNAGKF
NKLSAWESVYEDACDIVARIPVIAAFIYNLKYKGDKQIAIDPALDMGANFAHMIDQGDAYKDVARMYFILHSDHESGNVS
AHTTHLVHSALSDPYYAYAAGLNGLAGPLHGRANQEVLDWTIKFQEKYCKDQEPTKELITRALWDTLNSGQVIPGYGHAV
LRKTDPRYISQREFCQKHLPEDPLFKLVSMIFEVAPGVLTEHGKTKNPWPNVDAQSGVIQWHYGVKEWDFYTVLFGVGRA
LGCMANITWDRGLGYAIERPKSVTTPMLEEWAAKGGRL

Sequences:

>Translated_438_residues
MGLKETLKQKIEEFRPRTTRLVKEFGKVKIDEVTIEQCIGGARDIRSLVTDISYLDPQEGIRFRGKTIPETFAALPKAPG
SDYPTVESFWYFLLTGEVPTQAQVDEVLTEWKVRQEVPQYVFDTIRTLPRDSHPMAMLSVGITAMQRDSKFAALYNAGKF
NKLSAWESVYEDACDIVARIPVIAAFIYNLKYKGDKQIAIDPALDMGANFAHMIDQGDAYKDVARMYFILHSDHESGNVS
AHTTHLVHSALSDPYYAYAAGLNGLAGPLHGRANQEVLDWTIKFQEKYCKDQEPTKELITRALWDTLNSGQVIPGYGHAV
LRKTDPRYISQREFCQKHLPEDPLFKLVSMIFEVAPGVLTEHGKTKNPWPNVDAQSGVIQWHYGVKEWDFYTVLFGVGRA
LGCMANITWDRGLGYAIERPKSVTTPMLEEWAAKGGRL
>Mature_437_residues
GLKETLKQKIEEFRPRTTRLVKEFGKVKIDEVTIEQCIGGARDIRSLVTDISYLDPQEGIRFRGKTIPETFAALPKAPGS
DYPTVESFWYFLLTGEVPTQAQVDEVLTEWKVRQEVPQYVFDTIRTLPRDSHPMAMLSVGITAMQRDSKFAALYNAGKFN
KLSAWESVYEDACDIVARIPVIAAFIYNLKYKGDKQIAIDPALDMGANFAHMIDQGDAYKDVARMYFILHSDHESGNVSA
HTTHLVHSALSDPYYAYAAGLNGLAGPLHGRANQEVLDWTIKFQEKYCKDQEPTKELITRALWDTLNSGQVIPGYGHAVL
RKTDPRYISQREFCQKHLPEDPLFKLVSMIFEVAPGVLTEHGKTKNPWPNVDAQSGVIQWHYGVKEWDFYTVLFGVGRAL
GCMANITWDRGLGYAIERPKSVTTPMLEEWAAKGGRL

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=425, Percent_Identity=48.9411764705882, Blast_Score=431, Evalue=1e-121,
Organism=Escherichia coli, GI1786939, Length=443, Percent_Identity=27.9909706546275, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1786527, Length=331, Percent_Identity=23.5649546827795, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17555174, Length=425, Percent_Identity=48.9411764705882, Blast_Score=424, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6324328, Length=436, Percent_Identity=52.7522935779816, Blast_Score=465, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6319850, Length=432, Percent_Identity=53.0092592592593, Blast_Score=459, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6325257, Length=454, Percent_Identity=39.2070484581498, Blast_Score=323, Evalue=3e-89,
Organism=Drosophila melanogaster, GI24640126, Length=434, Percent_Identity=52.073732718894, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24640124, Length=434, Percent_Identity=52.073732718894, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI21356863, Length=427, Percent_Identity=42.3887587822014, Blast_Score=346, Evalue=2e-95,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 49460; Mature: 49329

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLKETLKQKIEEFRPRTTRLVKEFGKVKIDEVTIEQCIGGARDIRSLVTDISYLDPQEG
CCHHHHHHHHHHHHCCHHHHHHHHHCCCEEHHHHHHHHCCCHHHHHHHHHHHHHCCHHHC
IRFRGKTIPETFAALPKAPGSDYPTVESFWYFLLTGEVPTQAQVDEVLTEWKVRQEVPQY
CEECCCCCHHHHHHCCCCCCCCCCCHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
VFDTIRTLPRDSHPMAMLSVGITAMQRDSKFAALYNAGKFNKLSAWESVYEDACDIVARI
HHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
PVIAAFIYNLKYKGDKQIAIDPALDMGANFAHMIDQGDAYKDVARMYFILHSDHESGNVS
HHHHHHHHHHEECCCCEEEECCCHHCCCCHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCC
AHTTHLVHSALSDPYYAYAAGLNGLAGPLHGRANQEVLDWTIKFQEKYCKDQEPTKELIT
HHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
RALWDTLNSGQVIPGYGHAVLRKTDPRYISQREFCQKHLPEDPLFKLVSMIFEVAPGVLT
HHHHHHCCCCCCCCCCCCHHEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHH
EHGKTKNPWPNVDAQSGVIQWHYGVKEWDFYTVLFGVGRALGCMANITWDRGLGYAIERP
CCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCC
KSVTTPMLEEWAAKGGRL
CCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
GLKETLKQKIEEFRPRTTRLVKEFGKVKIDEVTIEQCIGGARDIRSLVTDISYLDPQEG
CHHHHHHHHHHHHCCHHHHHHHHHCCCEEHHHHHHHHCCCHHHHHHHHHHHHHCCHHHC
IRFRGKTIPETFAALPKAPGSDYPTVESFWYFLLTGEVPTQAQVDEVLTEWKVRQEVPQY
CEECCCCCHHHHHHCCCCCCCCCCCHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
VFDTIRTLPRDSHPMAMLSVGITAMQRDSKFAALYNAGKFNKLSAWESVYEDACDIVARI
HHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
PVIAAFIYNLKYKGDKQIAIDPALDMGANFAHMIDQGDAYKDVARMYFILHSDHESGNVS
HHHHHHHHHHEECCCCEEEECCCHHCCCCHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCC
AHTTHLVHSALSDPYYAYAAGLNGLAGPLHGRANQEVLDWTIKFQEKYCKDQEPTKELIT
HHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
RALWDTLNSGQVIPGYGHAVLRKTDPRYISQREFCQKHLPEDPLFKLVSMIFEVAPGVLT
HHHHHHCCCCCCCCCCCCHHEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHH
EHGKTKNPWPNVDAQSGVIQWHYGVKEWDFYTVLFGVGRALGCMANITWDRGLGYAIERP
CCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCC
KSVTTPMLEEWAAKGGRL
CCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7522844; 12948626 [H]