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The map label for this gene is prpD

Identifier: 78222339

GI number: 78222339

Start: 1242953

End: 1244398

Strand: Direct

Name: prpD

Synonym: Gmet_1123

Alternate gene names: 78222339

Gene position: 1242953-1244398 (Clockwise)

Preceding gene: 78222338

Following gene: 78222340

Centisome position: 31.09

GC content: 63.28

Gene sequence:

>1446_bases
GTGAGCACGACTGCCGACGTGAATGTCCGGCCAGCCCCGGACCAGGTGCTGGTCGACATAGCCGAGTACGTCACGGGCTA
CCGGGTGACGAGCCACGAGGCCCGCGAAACTGCCCGCTACTCCCTGATAGATGCCCTCGGGTGCGGCATGCTGGCCCTGA
CGTTTCCCGAGTGTACCAAGCTCCTGGGGCCGATCGTCCCCGGAATCGTCGTCCCTCTCGGCGCCCGGGTGCCGGGCACC
CGTTACGAGCTTGACCCGGTCAAGGCGGCCTTTGACATTGGCTGCATCATCCGGTGGCTCGACTTCAACGACACCTGGCT
GGCCGCCGAATGGGGACACCCGTCGGACAACCTCGGGGCGATCCTGGCTGTTGCCGACCACCTGAGCCGGCGCAACGTTG
CCGAAGGAAACGCTCCCGTGACCATGGGCGAGGTGTTGACCTGCATGGTCATGGCCCATGAAATCCAGGGGTGCCTGGCC
CTGGAGAACAGCTTCAACCGGGTGGGGCTCGACCACGTCCTGCTGGTCAAAGTCGCCTCGACCGCGGTTGTCATGAGACT
GCTCGGCGGGACAAAAGAGCAGATAATCGATGCCCTGTCCCAGGCGTGGGTCGACGGCCAGAGCCTCCGCACCTATCGCC
ATGCGCCCAATGCCGGCAGTCGCAAATCCTGGGCCGCGGGGGATGCCGTCAGCCGGGCCGTTCGGCTGGCCCTCATGACC
CTCCAGGGGGAGAAGGGGTATCCGAGCGCGCTCACCGCCCCCACGTGGGGATTCTATGACGTATCGTTCAAGGGAAGACC
GTTCCGCTTCCAGCGCCCCTTTGGCACCTACGTCATGGAAAACGTCCTCTTCAAGATCTCCTACCCTGCGGAATTCCATG
CCCAGACCGCCGTTGAGGCCGCAGTGAAGCTCCATCCCCAAGTGCGGGAGCGACTCTCGGACATCGAAAAGATCGTCATC
ACCACCCACGAATCCGCCATCCGCATCATCAGCAAGACCGGCAAACTCCATAACCCGGCGGACCGGGACCACTGCCTCCA
GTATATGACCGCCATCGGGCTCATTTTCGGGAACCTGACCGCCGACCATTACGAAGAGAAGGCTGCGGCCGATCCCCGCA
TCGATCCCCTGCGGGAGCGGATGGAGGTGGTGGAAGAGCCGCGTTACAGCCGCGAGTATCTTGAGCCGGACAAACGTTCC
ATCGCCAATGCCGTCCAGGTGTTCTTCCGTGACGGCTCCTGCACCGAGCGCGTGGAGGTGGAATATCCCCTGGGGCATCG
CCGCCGCCGTGCCGAGGGGATCCCACTCCTGCTGGAGAAATTCCGGGCGAACCTCTCTGCGCGTTTCCCGGAAGAACGGC
TCCGGCGGATAGTCGCCTGCTGCCAGGATCAGCAGCGGCTGGAGAATACTCCCGTGAATGAATTCATGGAATTGTTTCTT
ATCTGA

Upstream 100 bases:

>100_bases
AGGAACTTTACGAACTGCTCCACTATTACGACTATGAGCGGAAGCTCGACCGGCTGTCTTCCCGTGACGGCGTTGAAAGC
GGCGAGGGGAGGGAGAAACG

Downstream 100 bases:

>100_bases
TCATCGTCGAAAGGAGGTTGTGAAGTTGTGTAGCGTTGGTTGATTTTAATGCAATTAAGGAAAGGAGAGTTAGTACGATG
GGATTAAAAGAAACGCTTAA

Product: 2-methylcitrate dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MSTTADVNVRPAPDQVLVDIAEYVTGYRVTSHEARETARYSLIDALGCGMLALTFPECTKLLGPIVPGIVVPLGARVPGT
RYELDPVKAAFDIGCIIRWLDFNDTWLAAEWGHPSDNLGAILAVADHLSRRNVAEGNAPVTMGEVLTCMVMAHEIQGCLA
LENSFNRVGLDHVLLVKVASTAVVMRLLGGTKEQIIDALSQAWVDGQSLRTYRHAPNAGSRKSWAAGDAVSRAVRLALMT
LQGEKGYPSALTAPTWGFYDVSFKGRPFRFQRPFGTYVMENVLFKISYPAEFHAQTAVEAAVKLHPQVRERLSDIEKIVI
TTHESAIRIISKTGKLHNPADRDHCLQYMTAIGLIFGNLTADHYEEKAAADPRIDPLRERMEVVEEPRYSREYLEPDKRS
IANAVQVFFRDGSCTERVEVEYPLGHRRRRAEGIPLLLEKFRANLSARFPEERLRRIVACCQDQQRLENTPVNEFMELFL
I

Sequences:

>Translated_481_residues
MSTTADVNVRPAPDQVLVDIAEYVTGYRVTSHEARETARYSLIDALGCGMLALTFPECTKLLGPIVPGIVVPLGARVPGT
RYELDPVKAAFDIGCIIRWLDFNDTWLAAEWGHPSDNLGAILAVADHLSRRNVAEGNAPVTMGEVLTCMVMAHEIQGCLA
LENSFNRVGLDHVLLVKVASTAVVMRLLGGTKEQIIDALSQAWVDGQSLRTYRHAPNAGSRKSWAAGDAVSRAVRLALMT
LQGEKGYPSALTAPTWGFYDVSFKGRPFRFQRPFGTYVMENVLFKISYPAEFHAQTAVEAAVKLHPQVRERLSDIEKIVI
TTHESAIRIISKTGKLHNPADRDHCLQYMTAIGLIFGNLTADHYEEKAAADPRIDPLRERMEVVEEPRYSREYLEPDKRS
IANAVQVFFRDGSCTERVEVEYPLGHRRRRAEGIPLLLEKFRANLSARFPEERLRRIVACCQDQQRLENTPVNEFMELFL
I
>Mature_480_residues
STTADVNVRPAPDQVLVDIAEYVTGYRVTSHEARETARYSLIDALGCGMLALTFPECTKLLGPIVPGIVVPLGARVPGTR
YELDPVKAAFDIGCIIRWLDFNDTWLAAEWGHPSDNLGAILAVADHLSRRNVAEGNAPVTMGEVLTCMVMAHEIQGCLAL
ENSFNRVGLDHVLLVKVASTAVVMRLLGGTKEQIIDALSQAWVDGQSLRTYRHAPNAGSRKSWAAGDAVSRAVRLALMTL
QGEKGYPSALTAPTWGFYDVSFKGRPFRFQRPFGTYVMENVLFKISYPAEFHAQTAVEAAVKLHPQVRERLSDIEKIVIT
THESAIRIISKTGKLHNPADRDHCLQYMTAIGLIFGNLTADHYEEKAAADPRIDPLRERMEVVEEPRYSREYLEPDKRSI
ANAVQVFFRDGSCTERVEVEYPLGHRRRRAEGIPLLLEKFRANLSARFPEERLRRIVACCQDQQRLENTPVNEFMELFLI

Specific function: Catalyzes the dehydration of 2-methylcitrate to 2- methyl-cis-aconitate [H]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family [H]

Homologues:

Organism=Escherichia coli, GI1786528, Length=477, Percent_Identity=65.1991614255765, Blast_Score=663, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6325258, Length=488, Percent_Identity=54.9180327868852, Blast_Score=540, Evalue=1e-154,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012705
- InterPro:   IPR005656 [H]

Pfam domain/function: PF03972 MmgE_PrpD [H]

EC number: =4.2.1.79 [H]

Molecular weight: Translated: 53800; Mature: 53669

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTADVNVRPAPDQVLVDIAEYVTGYRVTSHEARETARYSLIDALGCGMLALTFPECTK
CCCCCCCEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LLGPIVPGIVVPLGARVPGTRYELDPVKAAFDIGCIIRWLDFNDTWLAAEWGHPSDNLGA
HHHHHHCHHHHCCCCCCCCCCEECCHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCHHH
ILAVADHLSRRNVAEGNAPVTMGEVLTCMVMAHEIQGCLALENSFNRVGLDHVLLVKVAS
HHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
TAVVMRLLGGTKEQIIDALSQAWVDGQSLRTYRHAPNAGSRKSWAAGDAVSRAVRLALMT
HHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
LQGEKGYPSALTAPTWGFYDVSFKGRPFRFQRPFGTYVMENVLFKISYPAEFHAQTAVEA
CCCCCCCCCCCCCCCCCEEEEEECCCCEEECCCHHHHHHHHHHEEECCCHHHHHHHHHHH
AVKLHPQVRERLSDIEKIVITTHESAIRIISKTGKLHNPADRDHCLQYMTAIGLIFGNLT
HHHCCHHHHHHHHHHHHEEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCC
ADHYEEKAAADPRIDPLRERMEVVEEPRYSREYLEPDKRSIANAVQVFFRDGSCTERVEV
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHCCCCCCCEEEE
EYPLGHRRRRAEGIPLLLEKFRANLSARFPEERLRRIVACCQDQQRLENTPVNEFMELFL
ECCCCHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
I
C
>Mature Secondary Structure 
STTADVNVRPAPDQVLVDIAEYVTGYRVTSHEARETARYSLIDALGCGMLALTFPECTK
CCCCCCEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LLGPIVPGIVVPLGARVPGTRYELDPVKAAFDIGCIIRWLDFNDTWLAAEWGHPSDNLGA
HHHHHHCHHHHCCCCCCCCCCEECCHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCHHH
ILAVADHLSRRNVAEGNAPVTMGEVLTCMVMAHEIQGCLALENSFNRVGLDHVLLVKVAS
HHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
TAVVMRLLGGTKEQIIDALSQAWVDGQSLRTYRHAPNAGSRKSWAAGDAVSRAVRLALMT
HHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
LQGEKGYPSALTAPTWGFYDVSFKGRPFRFQRPFGTYVMENVLFKISYPAEFHAQTAVEA
CCCCCCCCCCCCCCCCCEEEEEECCCCEEECCCHHHHHHHHHHEEECCCHHHHHHHHHHH
AVKLHPQVRERLSDIEKIVITTHESAIRIISKTGKLHNPADRDHCLQYMTAIGLIFGNLT
HHHCCHHHHHHHHHHHHEEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCC
ADHYEEKAAADPRIDPLRERMEVVEEPRYSREYLEPDKRSIANAVQVFFRDGSCTERVEV
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHCCCCCCCEEEE
EYPLGHRRRRAEGIPLLLEKFRANLSARFPEERLRRIVACCQDQQRLENTPVNEFMELFL
ECCCCHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
I
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]