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The map label for this gene is nth [H]

Identifier: 78188394

GI number: 78188394

Start: 487980

End: 488606

Strand: Reverse

Name: nth [H]

Synonym: Cag_0416

Alternate gene names: 78188394

Gene position: 488606-487980 (Counterclockwise)

Preceding gene: 78188395

Following gene: 78188386

Centisome position: 19.0

GC content: 48.01

Gene sequence:

>627_bases
ATGAACCCTCAAGAAAAAATTATAGCCTTGCACGATTTGCTTAGCAAGCAATTTCCCAATCCTAAAAGCGAATTGGAATA
TCTTTCGCCATTTCAATTGCTTATAGCCACCATTCTTGCCGCTCAAGCAACCGATAAACAGGTGAACGTTATTACGCGCG
AACTCTTTAAGCGTGCGCCTGATGCCATCACCATGAGCCGCATGGAGCTTGAAGAAATTACAGGCTATGTTCGTACCATT
AATTACTTCAATAACAAGGCAAAAAATATTCTTGAGGTAAGCCGCCGTCTCGTGGAACATTTTGGTGGAGAGGTGCCTCA
AGAGCGCGAAGCATTGGAAAGCTTGCCGGGTGTGGGGCGCAAAACCGCCAACGTTGTGCTTGCAAACGCCTTTGGAATGC
CCGTCATGGCGGTGGATACGCATGTTCACCGCGTTTCAAACCGCATCGGGTTAGTAAGCACAAAAAAAGTTGAAGCTACC
GAAGAGGCGCTTATGGCAATTATCCCCGAAGCATGGGTTGCCGACTTTCACCACTATTTGCTGTTGCATGGGCGCTACAC
CTGCAAAGCTAAAAAGCCTGCCTGCCCCACCTGCACCGTTGCCCATATTTGCGATTTTGCCGAGTAA

Upstream 100 bases:

>100_bases
GGTAAAGTGCATAAAAATGGACGTGGGTTAATTGATCAATCATTCAGCCGCCCACAAAAGCCTGAGAGTGAAGCAACCGA
GATAGAAGAATAACATTGCT

Downstream 100 bases:

>100_bases
GGTGGGTTCATGATTGTATTAACAACTCATACAAGTTGCCGAAATGAAGAGGCTTTAATTGCTGCACAAAGCTTTGTTCC
AACACTAACTTCTAATTCAT

Product: endonuclease III/Nth

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MNPQEKIIALHDLLSKQFPNPKSELEYLSPFQLLIATILAAQATDKQVNVITRELFKRAPDAITMSRMELEEITGYVRTI
NYFNNKAKNILEVSRRLVEHFGGEVPQEREALESLPGVGRKTANVVLANAFGMPVMAVDTHVHRVSNRIGLVSTKKVEAT
EEALMAIIPEAWVADFHHYLLLHGRYTCKAKKPACPTCTVAHICDFAE

Sequences:

>Translated_208_residues
MNPQEKIIALHDLLSKQFPNPKSELEYLSPFQLLIATILAAQATDKQVNVITRELFKRAPDAITMSRMELEEITGYVRTI
NYFNNKAKNILEVSRRLVEHFGGEVPQEREALESLPGVGRKTANVVLANAFGMPVMAVDTHVHRVSNRIGLVSTKKVEAT
EEALMAIIPEAWVADFHHYLLLHGRYTCKAKKPACPTCTVAHICDFAE
>Mature_208_residues
MNPQEKIIALHDLLSKQFPNPKSELEYLSPFQLLIATILAAQATDKQVNVITRELFKRAPDAITMSRMELEEITGYVRTI
NYFNNKAKNILEVSRRLVEHFGGEVPQEREALESLPGVGRKTANVVLANAFGMPVMAVDTHVHRVSNRIGLVSTKKVEAT
EEALMAIIPEAWVADFHHYLLLHGRYTCKAKKPACPTCTVAHICDFAE

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=181, Percent_Identity=29.2817679558011, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI115298648, Length=156, Percent_Identity=27.5641025641026, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI6912520, Length=156, Percent_Identity=27.5641025641026, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI115298650, Length=156, Percent_Identity=27.5641025641026, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI190358497, Length=156, Percent_Identity=27.5641025641026, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI115298654, Length=156, Percent_Identity=27.5641025641026, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI115298652, Length=156, Percent_Identity=27.5641025641026, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1787920, Length=195, Percent_Identity=48.7179487179487, Blast_Score=203, Evalue=8e-54,
Organism=Escherichia coli, GI1789331, Length=181, Percent_Identity=24.3093922651934, Blast_Score=68, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17554540, Length=175, Percent_Identity=30.8571428571429, Blast_Score=104, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6324530, Length=188, Percent_Identity=26.5957446808511, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45550361, Length=180, Percent_Identity=31.1111111111111, Blast_Score=97, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23322; Mature: 23322

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPQEKIIALHDLLSKQFPNPKSELEYLSPFQLLIATILAAQATDKQVNVITRELFKRAP
CCCHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
DAITMSRMELEEITGYVRTINYFNNKAKNILEVSRRLVEHFGGEVPQEREALESLPGVGR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCC
KTANVVLANAFGMPVMAVDTHVHRVSNRIGLVSTKKVEATEEALMAIIPEAWVADFHHYL
HHHHEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLHGRYTCKAKKPACPTCTVAHICDFAE
HHHCCEEECCCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MNPQEKIIALHDLLSKQFPNPKSELEYLSPFQLLIATILAAQATDKQVNVITRELFKRAP
CCCHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
DAITMSRMELEEITGYVRTINYFNNKAKNILEVSRRLVEHFGGEVPQEREALESLPGVGR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCC
KTANVVLANAFGMPVMAVDTHVHRVSNRIGLVSTKKVEATEEALMAIIPEAWVADFHHYL
HHHHEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLHGRYTCKAKKPACPTCTVAHICDFAE
HHHCCEEECCCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]