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The map label for this gene is dapF

Identifier: 78188320

GI number: 78188320

Start: 386816

End: 387628

Strand: Reverse

Name: dapF

Synonym: Cag_0342

Alternate gene names: 78188320

Gene position: 387628-386816 (Counterclockwise)

Preceding gene: 78188324

Following gene: 78188319

Centisome position: 15.07

GC content: 39.98

Gene sequence:

>813_bases
ATGAATATACCGTTCAAAAAACTATCAGGTGCAGGTAACGACTTTATTGTGATTGATAACCGTCATAATTCTATAGAGTT
CTCCACTGAAACAATTAAAGCTCTCTGTACTCGACGCACTGGTATTGGTGCTGATGGATTGATTTTAATTGAAGCCTCGC
TATCGGCTGACTTTACCATGAAGTATTACAATTCCGACGGACTGCTTGGCTCCATGTGTGGGAATGGTGGACGTTGCGCT
GCTTATTTTGCTTATAACAGCGGGGTGCCAACCCAAAAATCAAATGAGTACCTCTTTGAAGCCAACGGTAATTATTACCA
CGCATGGATAGTTGACAAAGAGCAAGTAAAATTACAAATGAACCCACCACATGATTTTAAAGAGAATTTACAAGTAGAGG
GATTGTTGTGTTATTTTTTAAATACAGGCTCTCCCCATGTTGTTACCTATGTTGATGATGTAAATATAATTAATGTTTAT
GAGAAAGGAGATGCTATACGCCACCGTACCGATATATTTAACGGTGGCACAAATGTGAATTTTGTACAACAAACCTCAGC
TAACGAGCTCATTATTCGCACCTTTGAACGAGGAGTAGAGGATGAAACACTTGCTTGCGGTACAGGAGCTGTTGCCTCTG
CATTAATCTCGCACATGCTCGGCAAAACCAACAGCACCTCTCTTAATGTTACCGTAAGAAGCGGTGATACCCTCCAAATA
TCATTTACCAGCGCTATGGAAAATATTTATCTTAGTGGACCCGCAAAAATTATTTTCAGCGGGACATTCGAAAAAACAAG
CAATTACGCTTAA

Upstream 100 bases:

>100_bases
GCAAAAAGATCAAACTTGAGATTAAGGAGAAAGTGCGTTTAAACACAATACTTGCATGTTAATTTAAAACAATAAACACA
ACAATAATACATTTTTTCTT

Downstream 100 bases:

>100_bases
TTATTACAAATAAATAAGTTAGACAAAACAAGAGATGCGATGTGGTACGTAAAAAAATCAAGCCCACAATATACCATGAA
TACATAAAGCAAGCTCTATT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MNIPFKKLSGAGNDFIVIDNRHNSIEFSTETIKALCTRRTGIGADGLILIEASLSADFTMKYYNSDGLLGSMCGNGGRCA
AYFAYNSGVPTQKSNEYLFEANGNYYHAWIVDKEQVKLQMNPPHDFKENLQVEGLLCYFLNTGSPHVVTYVDDVNIINVY
EKGDAIRHRTDIFNGGTNVNFVQQTSANELIIRTFERGVEDETLACGTGAVASALISHMLGKTNSTSLNVTVRSGDTLQI
SFTSAMENIYLSGPAKIIFSGTFEKTSNYA

Sequences:

>Translated_270_residues
MNIPFKKLSGAGNDFIVIDNRHNSIEFSTETIKALCTRRTGIGADGLILIEASLSADFTMKYYNSDGLLGSMCGNGGRCA
AYFAYNSGVPTQKSNEYLFEANGNYYHAWIVDKEQVKLQMNPPHDFKENLQVEGLLCYFLNTGSPHVVTYVDDVNIINVY
EKGDAIRHRTDIFNGGTNVNFVQQTSANELIIRTFERGVEDETLACGTGAVASALISHMLGKTNSTSLNVTVRSGDTLQI
SFTSAMENIYLSGPAKIIFSGTFEKTSNYA
>Mature_270_residues
MNIPFKKLSGAGNDFIVIDNRHNSIEFSTETIKALCTRRTGIGADGLILIEASLSADFTMKYYNSDGLLGSMCGNGGRCA
AYFAYNSGVPTQKSNEYLFEANGNYYHAWIVDKEQVKLQMNPPHDFKENLQVEGLLCYFLNTGSPHVVTYVDDVNIINVY
EKGDAIRHRTDIFNGGTNVNFVQQTSANELIIRTFERGVEDETLACGTGAVASALISHMLGKTNSTSLNVTVRSGDTLQI
SFTSAMENIYLSGPAKIIFSGTFEKTSNYA

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=274, Percent_Identity=30.2919708029197, Blast_Score=119, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 29614; Mature: 29614

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIPFKKLSGAGNDFIVIDNRHNSIEFSTETIKALCTRRTGIGADGLILIEASLSADFTM
CCCCCHHHCCCCCCEEEEECCCCCEEECHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEE
KYYNSDGLLGSMCGNGGRCAAYFAYNSGVPTQKSNEYLFEANGNYYHAWIVDKEQVKLQM
EEECCCCCEEECCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCEEEEEEEEHHHEEEEE
NPPHDFKENLQVEGLLCYFLNTGSPHVVTYVDDVNIINVYEKGDAIRHRTDIFNGGTNVN
CCCCCHHHCCEEEEEEEEEEECCCCEEEEEECCCEEEEEEECCCCCEEHHHEECCCCCEE
FVQQTSANELIIRTFERGVEDETLACGTGAVASALISHMLGKTNSTSLNVTVRSGDTLQI
EEEECCCCEEEEEEHHCCCCCCEEECCCCHHHHHHHHHHHCCCCCCEEEEEEECCCEEEE
SFTSAMENIYLSGPAKIIFSGTFEKTSNYA
EEECCCCEEEEECCEEEEEEECCCCCCCCC
>Mature Secondary Structure
MNIPFKKLSGAGNDFIVIDNRHNSIEFSTETIKALCTRRTGIGADGLILIEASLSADFTM
CCCCCHHHCCCCCCEEEEECCCCCEEECHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEE
KYYNSDGLLGSMCGNGGRCAAYFAYNSGVPTQKSNEYLFEANGNYYHAWIVDKEQVKLQM
EEECCCCCEEECCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCEEEEEEEEHHHEEEEE
NPPHDFKENLQVEGLLCYFLNTGSPHVVTYVDDVNIINVYEKGDAIRHRTDIFNGGTNVN
CCCCCHHHCCEEEEEEEEEEECCCCEEEEEECCCEEEEEEECCCCCEEHHHEECCCCCEE
FVQQTSANELIIRTFERGVEDETLACGTGAVASALISHMLGKTNSTSLNVTVRSGDTLQI
EEEECCCCEEEEEEHHCCCCCCEEECCCCHHHHHHHHHHHCCCCCCEEEEEEECCCEEEE
SFTSAMENIYLSGPAKIIFSGTFEKTSNYA
EEECCCCEEEEECCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]