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The map label for this gene is fusA [H]

Identifier: 78188324

GI number: 78188324

Start: 392155

End: 394230

Strand: Reverse

Name: fusA [H]

Synonym: Cag_0346

Alternate gene names: 78188324

Gene position: 394230-392155 (Counterclockwise)

Preceding gene: 78188337

Following gene: 78188320

Centisome position: 15.33

GC content: 44.46

Gene sequence:

>2076_bases
ATGCAACCCGTACCAACGGAACTACTGCGCAACATTGTACTTACTGGTCATGCCGGTAGCGGCAAAACCATTCTTGCCGA
ATCCTTTGCCCTTAGCATGGGCATTACAACGCGATTAGGTTCTATTGAAGAGGGTTCTACCCTTTCCGACTATTCGGCTG
ATGAAATTGAAAAAAAGCATAGTCTTAACACAACCATTATTCATGGTATGTGGAACAAGTACAAGCTCAATATTCTCGAT
ACTCCAGGCTTGCTTGATTTTCATGGCGACGTTAAAGCAGCCATGCGTGTTGCCGATACAGTTGTGCTGACGGTAAATGC
TTCGTCGGGCGTTGAGGTGGGAACTGATACTATTTGGAACTATACCCAAGAATATTACAAGCCAACAATTTTTGTGCTCA
CAAAACTTGATGCTGATAGAGCACACTTTAAAGAAACCATTGATGAGCTTAAAGAGCACTTTGGGCATTTAGTTACGCCA
CTCCAATTTCCTGCTGATGAAGGGCTTGGGCACCACACTTTGATTGATGTGTTGCTTATGAAGCAACTTGAGTTTAACCC
CGATAAACCGGGAGAAATGAACATTTCCGACATTCCCGATTTGTACGTTAAACAAGCTGAAGAGCTGCATCAGCAATTGG
TAGAAGCGGTGGCTGAAACTGACGAAGAGTTGATGAATAAGTTTTTTGAAGTTGGTAATTTAACTGAGGAAGAGTTGCGA
GATGGCATAAAATCAGCGTTAGTAACCCGCACCTTCTTCCCCGTTTTTTGCACCTCTCCCCTACACCTTATTGGCACAGA
ACGCTTGCTTCAAGCTATTACAAATCTCTGCCCATCACCCATTGAGCGTGGACCTGAAGAGGCAATTTGTACGCTGGATA
CTTCCCGACAACTTATCAACCCCGATCCAGAGGGAGAAACGGTCGCCTTTATTTTTAAGACCATGGCTGAACCCCGTGTT
GGCGAGGTATCGTACTTACGCGTCTATTCAGGACATATTGAAAGTGGGCATGAGTTGATTGACGCACAAACTGGTCAGCT
TGAAAAATTAGGACAAGTGCACGCCATGGTAGGACAAAAGAAAATCCCCGTTGAGAAGTTGCTGGCTGGCGACATTGGCA
TGGTGGTAAAGCTAAAAGATTCGCACACCAACGACACCTTAACCGATAAAGGATGCAGTTGTCGCATTAAGCCAATCAAC
TTTCCAACACCTGTGCTTGCAACAGCTATTTTGCCATTAGCCCAAGGTGATGAAGAAAAAATTTCAGCGGGACTTTATCA
CCTCCACGAAGAGGATCCAAGCTTTGCTATTGAACACAATGTGGAGTTTAACCAAACCATTATTAAAACACTTGGTGAAA
CGCACTTAGAAAGCATTATTAACCGTTTGCAAAGCAAGTTTAATGTAGCCGTTTCGCTCACGCCTATAAAAATTCCTTAT
CGGGAAACCATACGCCTTACAGCTTCAGCACAAGGCAAGTACAAAAAGCAGTCAGGTGGCAAAGGGCAATATGGCGATGT
ATGGGTGCGCATTGAACCACAAGAACGCGATAGTGGCTTTACGTTTGCAAGCGAAGTAGTTGGTGGCGTAGTTCCTACGC
GCTATTTGCCTGCCGTTGAAAAAGGGTTGCGCGAAACCATTGAGGGGGGATTGTTAGCTGGCTGCCCAATTGTGGATGTT
AAAGCGGTAGCATACGATGGCTCCTACCATCCTGTGGATAGTTCTGAATACGCTTTTAAAATTGCTGCAAGTATGGCACT
CAAAAGTGCTTTTGAAAAAGCAAAGCCCTTTTTATTAGAACCTATCTACACATTGGAAGTATATGCACCTGAACAATTTA
CGGGCGAGATTGTTGGAGAGATATCAAGCAAACGAGGAAAAATTCTTGGTATGGAAGCTGAGGTGAAAATGCAAATTATT
CATGCCCTTATTCCACAAGCAGCGCTCACACCATTTCACCATGCCTTAACACGGCTCACCCAAAGCCGTGCTCGTTACTC
GTATCACTTTAGCCATTACGAGGAGATGCCACCCGACTTAGCACAACAAATTATTGCGGAACGCAATCAACCTTAA

Upstream 100 bases:

>100_bases
GTGATAGTTCATGCCACAAAAAAAGCCTTTTGGCTATTGCGGTGCATGGAAAAATGCAATGGATATTCATTTTTACAACA
AGCAAAAAAGGAGTTACACC

Downstream 100 bases:

>100_bases
GAACAACTCACGTAATCAACTGATGCCCATCTAACCACCATAAGTTGCGTTGGATGGGCATGGGTTACAAGAGAGAATGA
ACACAACACGGCTCACTCAC

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 691; Mature: 691

Protein sequence:

>691_residues
MQPVPTELLRNIVLTGHAGSGKTILAESFALSMGITTRLGSIEEGSTLSDYSADEIEKKHSLNTTIIHGMWNKYKLNILD
TPGLLDFHGDVKAAMRVADTVVLTVNASSGVEVGTDTIWNYTQEYYKPTIFVLTKLDADRAHFKETIDELKEHFGHLVTP
LQFPADEGLGHHTLIDVLLMKQLEFNPDKPGEMNISDIPDLYVKQAEELHQQLVEAVAETDEELMNKFFEVGNLTEEELR
DGIKSALVTRTFFPVFCTSPLHLIGTERLLQAITNLCPSPIERGPEEAICTLDTSRQLINPDPEGETVAFIFKTMAEPRV
GEVSYLRVYSGHIESGHELIDAQTGQLEKLGQVHAMVGQKKIPVEKLLAGDIGMVVKLKDSHTNDTLTDKGCSCRIKPIN
FPTPVLATAILPLAQGDEEKISAGLYHLHEEDPSFAIEHNVEFNQTIIKTLGETHLESIINRLQSKFNVAVSLTPIKIPY
RETIRLTASAQGKYKKQSGGKGQYGDVWVRIEPQERDSGFTFASEVVGGVVPTRYLPAVEKGLRETIEGGLLAGCPIVDV
KAVAYDGSYHPVDSSEYAFKIAASMALKSAFEKAKPFLLEPIYTLEVYAPEQFTGEIVGEISSKRGKILGMEAEVKMQII
HALIPQAALTPFHHALTRLTQSRARYSYHFSHYEEMPPDLAQQIIAERNQP

Sequences:

>Translated_691_residues
MQPVPTELLRNIVLTGHAGSGKTILAESFALSMGITTRLGSIEEGSTLSDYSADEIEKKHSLNTTIIHGMWNKYKLNILD
TPGLLDFHGDVKAAMRVADTVVLTVNASSGVEVGTDTIWNYTQEYYKPTIFVLTKLDADRAHFKETIDELKEHFGHLVTP
LQFPADEGLGHHTLIDVLLMKQLEFNPDKPGEMNISDIPDLYVKQAEELHQQLVEAVAETDEELMNKFFEVGNLTEEELR
DGIKSALVTRTFFPVFCTSPLHLIGTERLLQAITNLCPSPIERGPEEAICTLDTSRQLINPDPEGETVAFIFKTMAEPRV
GEVSYLRVYSGHIESGHELIDAQTGQLEKLGQVHAMVGQKKIPVEKLLAGDIGMVVKLKDSHTNDTLTDKGCSCRIKPIN
FPTPVLATAILPLAQGDEEKISAGLYHLHEEDPSFAIEHNVEFNQTIIKTLGETHLESIINRLQSKFNVAVSLTPIKIPY
RETIRLTASAQGKYKKQSGGKGQYGDVWVRIEPQERDSGFTFASEVVGGVVPTRYLPAVEKGLRETIEGGLLAGCPIVDV
KAVAYDGSYHPVDSSEYAFKIAASMALKSAFEKAKPFLLEPIYTLEVYAPEQFTGEIVGEISSKRGKILGMEAEVKMQII
HALIPQAALTPFHHALTRLTQSRARYSYHFSHYEEMPPDLAQQIIAERNQP
>Mature_691_residues
MQPVPTELLRNIVLTGHAGSGKTILAESFALSMGITTRLGSIEEGSTLSDYSADEIEKKHSLNTTIIHGMWNKYKLNILD
TPGLLDFHGDVKAAMRVADTVVLTVNASSGVEVGTDTIWNYTQEYYKPTIFVLTKLDADRAHFKETIDELKEHFGHLVTP
LQFPADEGLGHHTLIDVLLMKQLEFNPDKPGEMNISDIPDLYVKQAEELHQQLVEAVAETDEELMNKFFEVGNLTEEELR
DGIKSALVTRTFFPVFCTSPLHLIGTERLLQAITNLCPSPIERGPEEAICTLDTSRQLINPDPEGETVAFIFKTMAEPRV
GEVSYLRVYSGHIESGHELIDAQTGQLEKLGQVHAMVGQKKIPVEKLLAGDIGMVVKLKDSHTNDTLTDKGCSCRIKPIN
FPTPVLATAILPLAQGDEEKISAGLYHLHEEDPSFAIEHNVEFNQTIIKTLGETHLESIINRLQSKFNVAVSLTPIKIPY
RETIRLTASAQGKYKKQSGGKGQYGDVWVRIEPQERDSGFTFASEVVGGVVPTRYLPAVEKGLRETIEGGLLAGCPIVDV
KAVAYDGSYHPVDSSEYAFKIAASMALKSAFEKAKPFLLEPIYTLEVYAPEQFTGEIVGEISSKRGKILGMEAEVKMQII
HALIPQAALTPFHHALTRLTQSRARYSYHFSHYEEMPPDLAQQIIAERNQP

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=697, Percent_Identity=29.8421807747489, Blast_Score=312, Evalue=6e-85,
Organism=Homo sapiens, GI19923640, Length=725, Percent_Identity=25.5172413793103, Blast_Score=244, Evalue=2e-64,
Organism=Homo sapiens, GI25306287, Length=725, Percent_Identity=24, Blast_Score=210, Evalue=4e-54,
Organism=Homo sapiens, GI25306283, Length=394, Percent_Identity=28.1725888324873, Blast_Score=152, Evalue=9e-37,
Organism=Escherichia coli, GI1789738, Length=700, Percent_Identity=35.1428571428571, Blast_Score=448, Evalue=1e-127,
Organism=Escherichia coli, GI1790835, Length=516, Percent_Identity=23.4496124031008, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI48994988, Length=166, Percent_Identity=30.1204819277108, Blast_Score=66, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17533571, Length=701, Percent_Identity=26.8188302425107, Blast_Score=277, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17556745, Length=717, Percent_Identity=22.8730822873082, Blast_Score=164, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6323098, Length=691, Percent_Identity=29.3777134587554, Blast_Score=306, Evalue=1e-83,
Organism=Saccharomyces cerevisiae, GI6322359, Length=815, Percent_Identity=24.5398773006135, Blast_Score=229, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24582462, Length=697, Percent_Identity=28.8378766140603, Blast_Score=313, Evalue=3e-85,
Organism=Drosophila melanogaster, GI221458488, Length=724, Percent_Identity=23.3425414364641, Blast_Score=197, Evalue=2e-50,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 76581; Mature: 76581

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPVPTELLRNIVLTGHAGSGKTILAESFALSMGITTRLGSIEEGSTLSDYSADEIEKKH
CCCCHHHHHHHHEEECCCCCCCEEEHHHHHHHHCHHHHCCCCCCCCCCCCCCHHHHHHHH
SLNTTIIHGMWNKYKLNILDTPGLLDFHGDVKAAMRVADTVVLTVNASSGVEVGTDTIWN
CCCCEEEECCCCEEEEEEECCCCCEECCCHHHHHHHHHCEEEEEEECCCCCCCCCHHHHH
YTQEYYKPTIFVLTKLDADRAHFKETIDELKEHFGHLVTPLQFPADEGLGHHTLIDVLLM
HHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
KQLEFNPDKPGEMNISDIPDLYVKQAEELHQQLVEAVAETDEELMNKFFEVGNLTEEELR
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHH
DGIKSALVTRTFFPVFCTSPLHLIGTERLLQAITNLCPSPIERGPEEAICTLDTSRQLIN
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEEECCCCCCCC
PDPEGETVAFIFKTMAEPRVGEVSYLRVYSGHIESGHELIDAQTGQLEKLGQVHAMVGQK
CCCCCCEEHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHCCC
KIPVEKLLAGDIGMVVKLKDSHTNDTLTDKGCSCRIKPINFPTPVLATAILPLAQGDEEK
CCCHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHH
ISAGLYHLHEEDPSFAIEHNVEFNQTIIKTLGETHLESIINRLQSKFNVAVSLTPIKIPY
HHHHHHHEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEEECCH
RETIRLTASAQGKYKKQSGGKGQYGDVWVRIEPQERDSGFTFASEVVGGVVPTRYLPAVE
HHEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEHHHHHCCCCCHHHHHHHH
KGLRETIEGGLLAGCPIVDVKAVAYDGSYHPVDSSEYAFKIAASMALKSAFEKAKPFLLE
HHHHHHHCCCCEECCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEC
PIYTLEVYAPEQFTGEIVGEISSKRGKILGMEAEVKMQIIHALIPQAALTPFHHALTRLT
CEEEEEEECCCHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSRARYSYHFSHYEEMPPDLAQQIIAERNQP
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MQPVPTELLRNIVLTGHAGSGKTILAESFALSMGITTRLGSIEEGSTLSDYSADEIEKKH
CCCCHHHHHHHHEEECCCCCCCEEEHHHHHHHHCHHHHCCCCCCCCCCCCCCHHHHHHHH
SLNTTIIHGMWNKYKLNILDTPGLLDFHGDVKAAMRVADTVVLTVNASSGVEVGTDTIWN
CCCCEEEECCCCEEEEEEECCCCCEECCCHHHHHHHHHCEEEEEEECCCCCCCCCHHHHH
YTQEYYKPTIFVLTKLDADRAHFKETIDELKEHFGHLVTPLQFPADEGLGHHTLIDVLLM
HHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
KQLEFNPDKPGEMNISDIPDLYVKQAEELHQQLVEAVAETDEELMNKFFEVGNLTEEELR
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHH
DGIKSALVTRTFFPVFCTSPLHLIGTERLLQAITNLCPSPIERGPEEAICTLDTSRQLIN
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEEECCCCCCCC
PDPEGETVAFIFKTMAEPRVGEVSYLRVYSGHIESGHELIDAQTGQLEKLGQVHAMVGQK
CCCCCCEEHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHCCC
KIPVEKLLAGDIGMVVKLKDSHTNDTLTDKGCSCRIKPINFPTPVLATAILPLAQGDEEK
CCCHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHH
ISAGLYHLHEEDPSFAIEHNVEFNQTIIKTLGETHLESIINRLQSKFNVAVSLTPIKIPY
HHHHHHHEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEEECCH
RETIRLTASAQGKYKKQSGGKGQYGDVWVRIEPQERDSGFTFASEVVGGVVPTRYLPAVE
HHEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEHHHHHCCCCCHHHHHHHH
KGLRETIEGGLLAGCPIVDVKAVAYDGSYHPVDSSEYAFKIAASMALKSAFEKAKPFLLE
HHHHHHHCCCCEECCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEC
PIYTLEVYAPEQFTGEIVGEISSKRGKILGMEAEVKMQIIHALIPQAALTPFHHALTRLT
CEEEEEEECCCHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSRARYSYHFSHYEEMPPDLAQQIIAERNQP
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]