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The map label for this gene is tig

Identifier: 78188219

GI number: 78188219

Start: 268432

End: 269754

Strand: Reverse

Name: tig

Synonym: Cag_0238

Alternate gene names: 78188219

Gene position: 269754-268432 (Counterclockwise)

Preceding gene: 78188220

Following gene: 78188218

Centisome position: 10.49

GC content: 44.22

Gene sequence:

>1323_bases
TTGCAAAAGACAATTACCCCCCTCAGCCCAACCGAACAGGAATTAGAAATTATTCTTTCCGCCGAAGAGTTCGGTCCCGA
ATACAATAAAGAGCTTGACGAAGCAAAACGCACCGTCCACATTAAAGGCTTCCGCAAAGGGCATGCGCCAATGGGCTTGC
TTAAAAAACTTGCTGGACCTTCTATTGAAATTGCTGTGGCTGAAAAACTTGGAGGCAAATATTTCACCGATATTATTACA
GCCGAAAACATTAAGCCTGCCAATCGTGCGCAAATTGCTGATTTCGCCTTTACGGATGACACCTTAACGCTCAAGTTGGT
CTATGAAATTCATCCTGAATTTGAGTTGCAAGATTTTAGCGGCTACACCTTTACCAAAGCAAACTACGTTGTAGGCGATA
AAGAGGTTGAACGCGAAATTGAGCTGATCCTTAAAAGCCATGGAACTATGGTAACAAGCGATGAGCCAGCAAGTGAAAAA
GATACCGTGATTGGCGATGCGCTGCGCTTTAACGACGAAGGCGAGCTTGATGAAGCCTCAAAAGTTGCCAACCACCACTT
TAGCATGGAATACCTTCCTGCTGAAAATCCCTTCCGCATTGCCCTTCTTGGCAAAAAAGCAGGCGATGTGGTTGAAGTAG
TAAACACGCCAATAAAAGAGGATGAAGAGGGCGAAGAGGCAACGGCAGAAGATGCTGAAGCCGTAAAGCCAACGCGCTTT
CAAGTAACCGTAACTGAGGTAAAGCGCCTTGAATTGCCTGAATTGGATGACGAGTTAGTCAAAGAAATTTCACAAGAACG
TTTTGATAACGTTGCTGACTTTAAAGCCGATGTTCGCTTGCAGCTTGAGCAGCACTTTACCATGAAATCCGACAACGACT
TGCTTGAAGCTATTTCAGGCAAAATGGTTGAAGAAAATCCCGTTCCCGTACCAAACTCGATGGTTGAATCCTTCCAAGAT
ATGTTGCTTGAAAATGCTAAGCGCCAACTTAGTGGCAACTTCCCAAAAGGTTTTGATGTTACGGGATTCCGTGCATCTAT
GCGCGACAACGCTGAAAAACATGCACGCTGGATGCTTATTACCCAAAAAGTAGCGGAAATGAATAAGCTAACCGTTACCG
ATGAGGATATTGTAGCGTTTGCTGAAAAAGAGGCTGGCAAAAATGAAGCGCTCGACATTAAGCAAATCATTGAAACCTAC
AAATCACCAGATTTCCACGATTACATTGCCGACTCTATTATGAAGGAAAAGGTTTACAATGCTATCACAGAAAAGGTAAC
CGTTACGGAAGAAGAGACGCCTGTTCCAGAGCACCGCATGTAA

Upstream 100 bases:

>100_bases
AAGAACGCAGATTTTGAAAAATGCGCTTTACTAAAAAAGAGGAATAATTCGTATCTTCACCATCCAAAATTTTCATTGAC
CAATCTTAACAACAACTGCC

Downstream 100 bases:

>100_bases
GAAACGCACACGCGCTCACCGTTTTTCCCCAACAAAAAAGCAGGAGTTTTAAAGCTCCTGCTTTTTTGTTGGGATCTATT
TGCTGAAAATGTATATTTTT

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MQKTITPLSPTEQELEIILSAEEFGPEYNKELDEAKRTVHIKGFRKGHAPMGLLKKLAGPSIEIAVAEKLGGKYFTDIIT
AENIKPANRAQIADFAFTDDTLTLKLVYEIHPEFELQDFSGYTFTKANYVVGDKEVEREIELILKSHGTMVTSDEPASEK
DTVIGDALRFNDEGELDEASKVANHHFSMEYLPAENPFRIALLGKKAGDVVEVVNTPIKEDEEGEEATAEDAEAVKPTRF
QVTVTEVKRLELPELDDELVKEISQERFDNVADFKADVRLQLEQHFTMKSDNDLLEAISGKMVEENPVPVPNSMVESFQD
MLLENAKRQLSGNFPKGFDVTGFRASMRDNAEKHARWMLITQKVAEMNKLTVTDEDIVAFAEKEAGKNEALDIKQIIETY
KSPDFHDYIADSIMKEKVYNAITEKVTVTEEETPVPEHRM

Sequences:

>Translated_440_residues
MQKTITPLSPTEQELEIILSAEEFGPEYNKELDEAKRTVHIKGFRKGHAPMGLLKKLAGPSIEIAVAEKLGGKYFTDIIT
AENIKPANRAQIADFAFTDDTLTLKLVYEIHPEFELQDFSGYTFTKANYVVGDKEVEREIELILKSHGTMVTSDEPASEK
DTVIGDALRFNDEGELDEASKVANHHFSMEYLPAENPFRIALLGKKAGDVVEVVNTPIKEDEEGEEATAEDAEAVKPTRF
QVTVTEVKRLELPELDDELVKEISQERFDNVADFKADVRLQLEQHFTMKSDNDLLEAISGKMVEENPVPVPNSMVESFQD
MLLENAKRQLSGNFPKGFDVTGFRASMRDNAEKHARWMLITQKVAEMNKLTVTDEDIVAFAEKEAGKNEALDIKQIIETY
KSPDFHDYIADSIMKEKVYNAITEKVTVTEEETPVPEHRM
>Mature_440_residues
MQKTITPLSPTEQELEIILSAEEFGPEYNKELDEAKRTVHIKGFRKGHAPMGLLKKLAGPSIEIAVAEKLGGKYFTDIIT
AENIKPANRAQIADFAFTDDTLTLKLVYEIHPEFELQDFSGYTFTKANYVVGDKEVEREIELILKSHGTMVTSDEPASEK
DTVIGDALRFNDEGELDEASKVANHHFSMEYLPAENPFRIALLGKKAGDVVEVVNTPIKEDEEGEEATAEDAEAVKPTRF
QVTVTEVKRLELPELDDELVKEISQERFDNVADFKADVRLQLEQHFTMKSDNDLLEAISGKMVEENPVPVPNSMVESFQD
MLLENAKRQLSGNFPKGFDVTGFRASMRDNAEKHARWMLITQKVAEMNKLTVTDEDIVAFAEKEAGKNEALDIKQIIETY
KSPDFHDYIADSIMKEKVYNAITEKVTVTEEETPVPEHRM

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=442, Percent_Identity=26.4705882352941, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_CHLCH (Q3AU11)

Other databases:

- EMBL:   CP000108
- RefSeq:   YP_378557.1
- ProteinModelPortal:   Q3AU11
- STRING:   Q3AU11
- GeneID:   3747898
- GenomeReviews:   CP000108_GR
- KEGG:   cch:Cag_0238
- eggNOG:   COG0544
- HOGENOM:   HBG405050
- OMA:   ETAINDG
- ProtClustDB:   CLSK638004
- BioCyc:   CCHL340177:CAG_0238-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 49635; Mature: 49635

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKTITPLSPTEQELEIILSAEEFGPEYNKELDEAKRTVHIKGFRKGHAPMGLLKKLAGP
CCCCCCCCCCCHHHHHEEEEHHHHCCHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHCCC
SIEIAVAEKLGGKYFTDIITAENIKPANRAQIADFAFTDDTLTLKLVYEIHPEFELQDFS
CEEEEEHHHHCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEEEEEEEECCCEEEECCC
GYTFTKANYVVGDKEVEREIELILKSHGTMVTSDEPASEKDTVIGDALRFNDEGELDEAS
CEEEECCEEEECCHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEHHEEECCCCCHHHHH
KVANHHFSMEYLPAENPFRIALLGKKAGDVVEVVNTPIKEDEEGEEATAEDAEAVKPTRF
HHHHHCCCEEEECCCCCEEEEEECCCCCCHHHHHCCCCCCCCCCCCCCCCHHHCCCCCEE
QVTVTEVKRLELPELDDELVKEISQERFDNVADFKADVRLQLEQHFTMKSDNDLLEAISG
EEEHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHCCHHEEEEEECCCCCCCCCHHHHHHCC
KMVEENPVPVPNSMVESFQDMLLENAKRQLSGNFPKGFDVTGFRASMRDNAEKHARWMLI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCHHHHHHHHHH
TQKVAEMNKLTVTDEDIVAFAEKEAGKNEALDIKQIIETYKSPDFHDYIADSIMKEKVYN
HHHHHHHHCEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AITEKVTVTEEETPVPEHRM
HHHHCEEECCCCCCCCCCCC
>Mature Secondary Structure
MQKTITPLSPTEQELEIILSAEEFGPEYNKELDEAKRTVHIKGFRKGHAPMGLLKKLAGP
CCCCCCCCCCCHHHHHEEEEHHHHCCHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHCCC
SIEIAVAEKLGGKYFTDIITAENIKPANRAQIADFAFTDDTLTLKLVYEIHPEFELQDFS
CEEEEEHHHHCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEEEEEEEECCCEEEECCC
GYTFTKANYVVGDKEVEREIELILKSHGTMVTSDEPASEKDTVIGDALRFNDEGELDEAS
CEEEECCEEEECCHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEHHEEECCCCCHHHHH
KVANHHFSMEYLPAENPFRIALLGKKAGDVVEVVNTPIKEDEEGEEATAEDAEAVKPTRF
HHHHHCCCEEEECCCCCEEEEEECCCCCCHHHHHCCCCCCCCCCCCCCCCHHHCCCCCEE
QVTVTEVKRLELPELDDELVKEISQERFDNVADFKADVRLQLEQHFTMKSDNDLLEAISG
EEEHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHCCHHEEEEEECCCCCCCCCHHHHHHCC
KMVEENPVPVPNSMVESFQDMLLENAKRQLSGNFPKGFDVTGFRASMRDNAEKHARWMLI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCHHHHHHHHHH
TQKVAEMNKLTVTDEDIVAFAEKEAGKNEALDIKQIIETYKSPDFHDYIADSIMKEKVYN
HHHHHHHHCEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AITEKVTVTEEETPVPEHRM
HHHHCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA