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The map label for this gene is ubiE

Identifier: 78067528

GI number: 78067528

Start: 3200102

End: 3200833

Strand: Reverse

Name: ubiE

Synonym: Bcep18194_A6059

Alternate gene names: 78067528

Gene position: 3200833-3200102 (Counterclockwise)

Preceding gene: 78067529

Following gene: 78067527

Centisome position: 86.65

GC content: 58.2

Gene sequence:

>732_bases
ATGAGCAAAACCCACTTCGGCTTCGAAAGCGTCGAGGAAAACGAAAAAGCGAAGAAAGTGGCGGGTGTGTTCCATTCGGT
CGCGAGCAACTACGATCTGATGAACGACCTGATGTCGGCGGGCATGCACCGCGCGTGGAAGGCGTTCACGATCGCGCAGG
CGAACGTGCGCCCCGGCTTCAAGGTACTCGACCTCGCGGCAGGCACCGGCGACCTGACCAAGGCGTTCGCGAAGGCGGCC
GGGCCGACGGGCGAGGTCTGGCACACGGACATCAACGAATCGATGCTGCGCGTCGGCCGCGACCGGCTGCTCGACAAGGG
TGTCGTGACGCCGTCGCTGCTGTGCGACGCGGAGAAAATTCCCTTTCCGGACAACTACTTCGATCTGGTCACGGTCGCGT
TCGGGCTGCGCAACATGACGCATAAGGATGCCGCTCTGGCCGAGATGCGCCGTGTGACGAAGCCCGGCGGCCGCGTGATG
GTGCTGGAATTCTCGAAAGTCTGGGATCCGCTGAAAAAGGCGTACGATCTGTATTCTTTCAAAGTATTACCGTGGCTTGG
CGACAAGTTCGCGAAAGATGCTGAAAGTTATCGGTATCTTGCTGAATCTATCCGGATGCACCCCGATCAGGACACGCTGA
AGACGATGATGGAACAAGCGGGCCTCGATGCCGTCAAATATTACAATTTGTCAGGTGGCGTGGTAGCTTTACACATGGGA
GCCAAGTACTAA

Upstream 100 bases:

>100_bases
ACGCCCCGATGCAGGCGGCGAGCGCGTCGCACGGCCACTGCCACTGAAATTCGACGGCGCCCGCTCGGCGCCGTCTTGCC
ATTTACTGAGGATCAACGCG

Downstream 100 bases:

>100_bases
GGGGCTCTTTCCATACATTTGTCTTACATCTGGAGAAACTGATGTTCGAATCGCGTTCGTTGTTCAACCGTAGCAAGCCG
TCGAAGCCGTGGGCTCGACG

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MSKTHFGFESVEENEKAKKVAGVFHSVASNYDLMNDLMSAGMHRAWKAFTIAQANVRPGFKVLDLAAGTGDLTKAFAKAA
GPTGEVWHTDINESMLRVGRDRLLDKGVVTPSLLCDAEKIPFPDNYFDLVTVAFGLRNMTHKDAALAEMRRVTKPGGRVM
VLEFSKVWDPLKKAYDLYSFKVLPWLGDKFAKDAESYRYLAESIRMHPDQDTLKTMMEQAGLDAVKYYNLSGGVVALHMG
AKY

Sequences:

>Translated_243_residues
MSKTHFGFESVEENEKAKKVAGVFHSVASNYDLMNDLMSAGMHRAWKAFTIAQANVRPGFKVLDLAAGTGDLTKAFAKAA
GPTGEVWHTDINESMLRVGRDRLLDKGVVTPSLLCDAEKIPFPDNYFDLVTVAFGLRNMTHKDAALAEMRRVTKPGGRVM
VLEFSKVWDPLKKAYDLYSFKVLPWLGDKFAKDAESYRYLAESIRMHPDQDTLKTMMEQAGLDAVKYYNLSGGVVALHMG
AKY
>Mature_242_residues
SKTHFGFESVEENEKAKKVAGVFHSVASNYDLMNDLMSAGMHRAWKAFTIAQANVRPGFKVLDLAAGTGDLTKAFAKAAG
PTGEVWHTDINESMLRVGRDRLLDKGVVTPSLLCDAEKIPFPDNYFDLVTVAFGLRNMTHKDAALAEMRRVTKPGGRVMV
LEFSKVWDPLKKAYDLYSFKVLPWLGDKFAKDAESYRYLAESIRMHPDQDTLKTMMEQAGLDAVKYYNLSGGVVALHMGA
KY

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Homo sapiens, GI116063536, Length=277, Percent_Identity=43.3212996389892, Blast_Score=220, Evalue=1e-57,
Organism=Escherichia coli, GI48994983, Length=243, Percent_Identity=61.7283950617284, Blast_Score=305, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI17557039, Length=244, Percent_Identity=45.4918032786885, Blast_Score=216, Evalue=6e-57,
Organism=Saccharomyces cerevisiae, GI6323526, Length=257, Percent_Identity=40.4669260700389, Blast_Score=162, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24641702, Length=255, Percent_Identity=45.0980392156863, Blast_Score=219, Evalue=1e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27079; Mature: 26948

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS01183 UBIE_1 ; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTHFGFESVEENEKAKKVAGVFHSVASNYDLMNDLMSAGMHRAWKAFTIAQANVRPGF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
KVLDLAAGTGDLTKAFAKAAGPTGEVWHTDINESMLRVGRDRLLDKGVVTPSLLCDAEKI
EEEEECCCCCHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCC
PFPDNYFDLVTVAFGLRNMTHKDAALAEMRRVTKPGGRVMVLEFSKVWDPLKKAYDLYSF
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHE
KVLPWLGDKFAKDAESYRYLAESIRMHPDQDTLKTMMEQAGLDAVKYYNLSGGVVALHMG
EECHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEECCCCCEEEEECC
AKY
CCC
>Mature Secondary Structure 
SKTHFGFESVEENEKAKKVAGVFHSVASNYDLMNDLMSAGMHRAWKAFTIAQANVRPGF
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
KVLDLAAGTGDLTKAFAKAAGPTGEVWHTDINESMLRVGRDRLLDKGVVTPSLLCDAEKI
EEEEECCCCCHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCC
PFPDNYFDLVTVAFGLRNMTHKDAALAEMRRVTKPGGRVMVLEFSKVWDPLKKAYDLYSF
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHE
KVLPWLGDKFAKDAESYRYLAESIRMHPDQDTLKTMMEQAGLDAVKYYNLSGGVVALHMG
EECHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEECCCCCEEEEECC
AKY
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA