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The map label for this gene is rnfB [H]

Identifier: 78067137

GI number: 78067137

Start: 2775767

End: 2776771

Strand: Direct

Name: rnfB [H]

Synonym: Bcep18194_A5668

Alternate gene names: 78067137

Gene position: 2775767-2776771 (Clockwise)

Preceding gene: 78067135

Following gene: 78067138

Centisome position: 75.14

GC content: 70.15

Gene sequence:

>1005_bases
ATGCGGGAAATCGGCGATAATCGAGCTTTGGCAAACCTTTCCGACCGCGTCCGACGCGGCCGAGAGCCATTCGGCGCCGC
TGCGCCCGTTGTCACCGTGACCGATTCCAAAACACTCGCAGATCGCATCGAAGATCTGCTTCCCCAGACGCAATGCACGA
AGTGCGGCTATAACGGCTGCCGCCCGTACGCCGAGGCAATCGCCGCCGGCGACGCGAACTACAACCAGTGCCCGCCCGGC
GGCGCCGAAGGCATCGCGCGCCTGTCGAGCCTGCTCGGCAAACCGGTGATTCCGCTGAACCCCGTCAACGGCAGCGAGCA
TCCGCGCGCGGTCGCCTTCATCGACGAAAGCCTGTGCATCGGCTGCACGCTGTGCATGCAGGCGTGCCCGGTCGACGCGA
TCGTCGGCGCGCCGAAGCAGATGCACACGATCATCGAATCGCTGTGCACCGGTTGCGACCTGTGCGTGCCGCCCTGCCCG
GTCGACTGCATCGCGATGCTGCCGGTGACCGGCGAACGCACCGGCTGGGACGCGTGGTCGCAGGAACAGGCCGATGCCGC
GCGCGAACGCCACGACCTGCGGCTCGCGCGCCAGCGCCGCGAACGCGAGGCCGCCGAAGCGCGCGCGGCCGCACGCCGCG
CGGCCAGTGCCGCGAAGCCGGCTGCGGCCCAGGCGGAAACGCAATCCGCCGCGCCGGCCGCCGCACCGGCCGCAGACGAC
GCCGAAGCGAAGAAGCGCGCGATCATCGCCGCCGCGCTCGAGCGGGCGCGCAAGAAGAAGGAAGAGCTGTCCGGGCAAGA
CGCCGGGCCGAAGAACACCGAAGGCGTGAGCGCAGCCGTCCAGGCGCAGATCGATGCGGCCGAGGCGCGCCGCAAACGGC
TCGCCGAACAGCAGGCCCAACGCGACGCCGAAGCGGCGGCCGCGAACGGCAATGACAAAGAGAACGACGCAGGCAACGAC
GGCCCCGGTGGCCCGTCCGCACCGCCCGACCAGAACGCTCCATGA

Upstream 100 bases:

>100_bases
CGCCGGCCGCCCTGGAACGGGCGCGCCGCGGGCGGCCGCCCTCCTGCTGAGCCCCTGCAAAAAAGCGGTTCGATTATGCG
CACAGACGGCGTCCAGCGCA

Downstream 100 bases:

>100_bases
ACGCCAGCAAACGACGCGCGATCTACGAAACGCTGCAGAGCCTCAATCCGCATCCGACCACCGAGCTCGAATACTCGACG
CCGTTCGAGTTGCTGATCGC

Product: ferredoxin

Products: NA

Alternate protein names: Nitrogen fixation protein rnfB [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MREIGDNRALANLSDRVRRGREPFGAAAPVVTVTDSKTLADRIEDLLPQTQCTKCGYNGCRPYAEAIAAGDANYNQCPPG
GAEGIARLSSLLGKPVIPLNPVNGSEHPRAVAFIDESLCIGCTLCMQACPVDAIVGAPKQMHTIIESLCTGCDLCVPPCP
VDCIAMLPVTGERTGWDAWSQEQADAARERHDLRLARQRREREAAEARAAARRAASAAKPAAAQAETQSAAPAAAPAADD
AEAKKRAIIAAALERARKKKEELSGQDAGPKNTEGVSAAVQAQIDAAEARRKRLAEQQAQRDAEAAAANGNDKENDAGND
GPGGPSAPPDQNAP

Sequences:

>Translated_334_residues
MREIGDNRALANLSDRVRRGREPFGAAAPVVTVTDSKTLADRIEDLLPQTQCTKCGYNGCRPYAEAIAAGDANYNQCPPG
GAEGIARLSSLLGKPVIPLNPVNGSEHPRAVAFIDESLCIGCTLCMQACPVDAIVGAPKQMHTIIESLCTGCDLCVPPCP
VDCIAMLPVTGERTGWDAWSQEQADAARERHDLRLARQRREREAAEARAAARRAASAAKPAAAQAETQSAAPAAAPAADD
AEAKKRAIIAAALERARKKKEELSGQDAGPKNTEGVSAAVQAQIDAAEARRKRLAEQQAQRDAEAAAANGNDKENDAGND
GPGGPSAPPDQNAP
>Mature_334_residues
MREIGDNRALANLSDRVRRGREPFGAAAPVVTVTDSKTLADRIEDLLPQTQCTKCGYNGCRPYAEAIAAGDANYNQCPPG
GAEGIARLSSLLGKPVIPLNPVNGSEHPRAVAFIDESLCIGCTLCMQACPVDAIVGAPKQMHTIIESLCTGCDLCVPPCP
VDCIAMLPVTGERTGWDAWSQEQADAARERHDLRLARQRREREAAEARAAARRAASAAKPAAAQAETQSAAPAAAPAADD
AEAKKRAIIAAALERARKKKEELSGQDAGPKNTEGVSAAVQAQIDAAEARRKRLAEQQAQRDAEAAAANGNDKENDAGND
GPGGPSAPPDQNAP

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase [H]

COG id: COG2878

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfB

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787915, Length=142, Percent_Identity=50.7042253521127, Blast_Score=144, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR010207
- InterPro:   IPR016463
- InterPro:   IPR007202
- InterPro:   IPR012285 [H]

Pfam domain/function: PF00037 Fer4; PF04060 FeS [H]

EC number: NA

Molecular weight: Translated: 34952; Mature: 34952

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00198 4FE4S_FERREDOXIN ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREIGDNRALANLSDRVRRGREPFGAAAPVVTVTDSKTLADRIEDLLPQTQCTKCGYNGC
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCC
RPYAEAIAAGDANYNQCPPGGAEGIARLSSLLGKPVIPLNPVNGSEHPRAVAFIDESLCI
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCHHHH
GCTLCMQACPVDAIVGAPKQMHTIIESLCTGCDLCVPPCPVDCIAMLPVTGERTGWDAWS
HHHHHHHHCCHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCC
QEQADAARERHDLRLARQRREREAAEARAAARRAASAAKPAAAQAETQSAAPAAAPAADD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCC
AEAKKRAIIAAALERARKKKEELSGQDAGPKNTEGVSAAVQAQIDAAEARRKRLAEQQAQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RDAEAAAANGNDKENDAGNDGPGGPSAPPDQNAP
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MREIGDNRALANLSDRVRRGREPFGAAAPVVTVTDSKTLADRIEDLLPQTQCTKCGYNGC
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCC
RPYAEAIAAGDANYNQCPPGGAEGIARLSSLLGKPVIPLNPVNGSEHPRAVAFIDESLCI
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCHHHH
GCTLCMQACPVDAIVGAPKQMHTIIESLCTGCDLCVPPCPVDCIAMLPVTGERTGWDAWS
HHHHHHHHCCHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCC
QEQADAARERHDLRLARQRREREAAEARAAARRAASAAKPAAAQAETQSAAPAAAPAADD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCC
AEAKKRAIIAAALERARKKKEELSGQDAGPKNTEGVSAAVQAQIDAAEARRKRLAEQQAQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RDAEAAAANGNDKENDAGNDGPGGPSAPPDQNAP
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA