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The map label for this gene is cysD

Identifier: 78066964

GI number: 78066964

Start: 2582307

End: 2583203

Strand: Direct

Name: cysD

Synonym: Bcep18194_A5495

Alternate gene names: 78066964

Gene position: 2582307-2583203 (Clockwise)

Preceding gene: 78066959

Following gene: 78066965

Centisome position: 69.9

GC content: 64.44

Gene sequence:

>897_bases
ATGTTGACCCACTTGGAGCGGCTGGAGGCCGAAAGCATCCACATCATGCGCGAGGTGGTCGCCGAGAGCGAAAACCCGGT
GATGCTGTATTCGATCGGCAAGGACAGCTCGGTCATGCTGCATCTCGCGATGAAGGCGTTCTACCCGGCGAAGCCGCCCT
TTCCGCTGCTGCACGTCGATACGACCTGGAAATTCCGCGAGATGATCGCGTTTCGCGACGAGACGGCCGCGCGCCTCGGC
CTCGACCTGCGCGTGCACATCAACCCCGACGGCGTCGCGAACGGCATCGATCCGTTCACGCACGGCTCGGCCGTGCACAC
CGACGTGTGGAAGACGCAAGGGCTCAAGCAGGCGCTCGATCACTACGGCTTCGACGCCGCGTTCGGCGGCGCCCGCCGCG
ACGAGGAAAAATCGCGCGCGAAGGAGCGCATCGTGTCGCTGCGCTCGGAACAGCACCGCTGGGACCCGAAGCGCCAGCGC
CCCGAGCTCTGGTCGCTGTACAACGCGCGCAAGCGCAAGGGCGAAAGCCTGCGCGTGTTCCCGATCTCGAACTGGACCGA
GCTCGACATCTGGCAGTACATCCAGCTTCACGACATCCCGATCGTCCCGCTCTACTTCGCGAAGGAACGGCCCGTGGTCG
AACGCGACGGCGCGCTGATCATGGTCGACGACGAGCGCCTGCCGCTGCGCGAAGGCGAAGTGCCGCAGATGCGCAAGGTG
CGCTTTCGCACGCTCGGCTGCTATCCGCTGACGGGTGCGATCGACAGCGACGCGACGTCGCTCGACGACATCCTGCAGGA
GATGCGCGAGACGCGCACGTCCGAACGGCAAGGACGACTGATCGACAGCGATTCGGCCGGTTCGATGGAAAAGAAGAAAC
AGGAGGGATATTTCTGA

Upstream 100 bases:

>100_bases
CCGGACGAAGATCGGCATGCCGCTCGCTCGATACCGTCGGTGTCATGCACGAAACGCCGCACATGTCGCGCGGCACCGGC
ATGAAACAAGGAGACGAACG

Downstream 100 bases:

>100_bases
TGGCACACGTTCTCACCGCGCCCGAGGCGCTCGCGCACCATGCACCCGATTCACACGCTGCCGACGAAGCGGACACGCCA
ACCCAGGACCTGTTGCGCTT

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MLTHLERLEAESIHIMREVVAESENPVMLYSIGKDSSVMLHLAMKAFYPAKPPFPLLHVDTTWKFREMIAFRDETAARLG
LDLRVHINPDGVANGIDPFTHGSAVHTDVWKTQGLKQALDHYGFDAAFGGARRDEEKSRAKERIVSLRSEQHRWDPKRQR
PELWSLYNARKRKGESLRVFPISNWTELDIWQYIQLHDIPIVPLYFAKERPVVERDGALIMVDDERLPLREGEVPQMRKV
RFRTLGCYPLTGAIDSDATSLDDILQEMRETRTSERQGRLIDSDSAGSMEKKKQEGYF

Sequences:

>Translated_298_residues
MLTHLERLEAESIHIMREVVAESENPVMLYSIGKDSSVMLHLAMKAFYPAKPPFPLLHVDTTWKFREMIAFRDETAARLG
LDLRVHINPDGVANGIDPFTHGSAVHTDVWKTQGLKQALDHYGFDAAFGGARRDEEKSRAKERIVSLRSEQHRWDPKRQR
PELWSLYNARKRKGESLRVFPISNWTELDIWQYIQLHDIPIVPLYFAKERPVVERDGALIMVDDERLPLREGEVPQMRKV
RFRTLGCYPLTGAIDSDATSLDDILQEMRETRTSERQGRLIDSDSAGSMEKKKQEGYF
>Mature_298_residues
MLTHLERLEAESIHIMREVVAESENPVMLYSIGKDSSVMLHLAMKAFYPAKPPFPLLHVDTTWKFREMIAFRDETAARLG
LDLRVHINPDGVANGIDPFTHGSAVHTDVWKTQGLKQALDHYGFDAAFGGARRDEEKSRAKERIVSLRSEQHRWDPKRQR
PELWSLYNARKRKGESLRVFPISNWTELDIWQYIQLHDIPIVPLYFAKERPVVERDGALIMVDDERLPLREGEVPQMRKV
RFRTLGCYPLTGAIDSDATSLDDILQEMRETRTSERQGRLIDSDSAGSMEKKKQEGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily

Homologues:

Organism=Escherichia coli, GI1789109, Length=297, Percent_Identity=71.3804713804714, Blast_Score=440, Evalue=1e-125,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSD_BURS3 (Q39EM7)

Other databases:

- EMBL:   CP000151
- RefSeq:   YP_369733.1
- HSSP:   Q87WW0
- ProteinModelPortal:   Q39EM7
- SMR:   Q39EM7
- STRING:   Q39EM7
- GeneID:   3750716
- GenomeReviews:   CP000151_GR
- KEGG:   bur:Bcep18194_A5495
- NMPDR:   fig|269483.3.peg.6711
- eggNOG:   COG0175
- HOGENOM:   HBG758022
- OMA:   HYGFDAA
- ProtClustDB:   PRK12563
- BioCyc:   BSP36773:BCEP18194_A5495-MONOMER
- HAMAP:   MF_00064
- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF002936
- TIGRFAMs:   TIGR02039

Pfam domain/function: PF01507 PAPS_reduct

EC number: =2.7.7.4

Molecular weight: Translated: 34445; Mature: 34445

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTHLERLEAESIHIMREVVAESENPVMLYSIGKDSSVMLHLAMKAFYPAKPPFPLLHVD
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEC
TTWKFREMIAFRDETAARLGLDLRVHINPDGVANGIDPFTHGSAVHTDVWKTQGLKQALD
CCHHHHHHHHHHCCHHHHHCCEEEEEECCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHH
HYGFDAAFGGARRDEEKSRAKERIVSLRSEQHRWDPKRQRPELWSLYNARKRKGESLRVF
HCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEE
PISNWTELDIWQYIQLHDIPIVPLYFAKERPVVERDGALIMVDDERLPLREGEVPQMRKV
ECCCCCCCHHHHHHHHCCCCEEEEEECCCCCCEECCCCEEEECCCCCCCCCCCCCHHHHH
RFRTLGCYPLTGAIDSDATSLDDILQEMRETRTSERQGRLIDSDSAGSMEKKKQEGYF
HHHEECCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHCCCC
>Mature Secondary Structure
MLTHLERLEAESIHIMREVVAESENPVMLYSIGKDSSVMLHLAMKAFYPAKPPFPLLHVD
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEC
TTWKFREMIAFRDETAARLGLDLRVHINPDGVANGIDPFTHGSAVHTDVWKTQGLKQALD
CCHHHHHHHHHHCCHHHHHCCEEEEEECCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHH
HYGFDAAFGGARRDEEKSRAKERIVSLRSEQHRWDPKRQRPELWSLYNARKRKGESLRVF
HCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEE
PISNWTELDIWQYIQLHDIPIVPLYFAKERPVVERDGALIMVDDERLPLREGEVPQMRKV
ECCCCCCCHHHHHHHHCCCCEEEEEECCCCCCEECCCCEEEECCCCCCCCCCCCCHHHHH
RFRTLGCYPLTGAIDSDATSLDDILQEMRETRTSERQGRLIDSDSAGSMEKKKQEGYF
HHHEECCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA