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The map label for this gene is pleD [H]

Identifier: 78066673

GI number: 78066673

Start: 2250104

End: 2251504

Strand: Reverse

Name: pleD [H]

Synonym: Bcep18194_A5204

Alternate gene names: 78066673

Gene position: 2251504-2250104 (Counterclockwise)

Preceding gene: 78066674

Following gene: 78066671

Centisome position: 60.95

GC content: 65.1

Gene sequence:

>1401_bases
ATGAGTACGACCTATGCAGCCGGGGAACCGCCGCGCGTCGTCAGCGCGGCCGCGAGGCTCTACGCGAAATGCCTGCTGAT
TGGATTCGCGTTGCTGCCAGCCTATCTGATCGCCTATCTGTGGTTCTTCAGCGACCCGCACGTCGTGTTCGAGAGCCACG
CGTTCCATGAAATCGCAATCGCTGCCGCGACCCTCGAAGGCGCGTTCGTCACCTACGTGACGTGGGTGTGCTACCGCTCA
TCTGGCGAGCCGCTGCTGCGCTGGCTGACACTTGGCTTTCTCGGCTTCGCGATGATCTACGCGCTGCACGGTGCGTTCAC
CGGAATGGCGCATCACAACATCTGGCTGTTCCTGCTGTACGGCCCCGCGTCGCGGCTCGTGATGTCGATCCTGCTGCTCA
CGGGCCTGCTGTCGTATTCGCGTCCGCCCGATCGGATCGAGAGGCGCACCAGCGTGCGCACGTGGCTGCCGTGGATCGTG
TTTTTCGTGATCGTGAACGTGGTCGTCGCGTACATCGCGTATTCACCGGTCGCGGGCGCGCTGGGCACACGGCTGTCGAT
GGAGGGCGGTGCGCTCGTGTTCTCGCTGCTGAACGTCGCCGTGCTGCTCGCGCGGCGGATCCGCTCGCCGCTGATGGTGA
TCTACGGCGTGTCGATCATGGCGTTCGCGCTGTCGTCGCTCGCGTTCATCCTCGGCAAGCCGTGGAATCACATGTGGTGG
CTCGCGCATGCGATTTTCGCCGGCGGCTTCTTCCTGCTCAGCTACGGTGTCGTGCAGGCGCTGCAGACGACGCGCTCATT
CTCGGCGATCTACAGCCAGGAAGACCTGATGAGCCGGCTGTCGGAATCGATGGCGCGTACCGAGAGCGCGCTGCAGGAGT
TGCGGCGCACGAACCAGAAGCTCGAATACATGGCCGCGACCGACCCGATGACGGGTGCGTCGAACCGCCGGCAGTTCATC
GCGCAGGTCGAGCGCGAGATGGTGCGGGCCGAGCGTGACGGCACACCGTTCTGCCTGCTCGCGCTCGATCTCGACAATTT
CAAGAACATCAACGATGCGTACGGGCACCAGATCGGCGACGAGGTGCTGCGCGGCGTCGTACGCCAGTGCCTCGATGCGA
TCCGGCCGGCGGGCGGGCTGGCACGCGTCGGCGGCGAGGAGTTCATGGCGTTGCTGCCGGATATGCCGCTCGAAGGCGCG
CGGATGACGGCCGAGCGCGTGCGCTCGTCGATCGCGAGCTCGCCATTCGGGCTCGATTTCAAGCGCGTACAGGTGACGGT
CAGCGTGGGTGTCGCGCAATACGGGGCCGACGGCAGCACGGTGGATGCGTTGCTGCGCGTGGTCGACGAACGCTTGTATC
AGGCGAAACGTGAAGGGCGGAACCGGGTCGTGTCGCGGTGA

Upstream 100 bases:

>100_bases
TGCTCTGACATTTGCCTTTCGGCACGATGGGCCGGATCATGCGTCGCGCGATCCGTGCCGCGAAGGTTCGAACCAACGCA
GCAGACCGACCGGGGACCGA

Downstream 100 bases:

>100_bases
CAGCGGTTTGCGCGGCACGCAGGTACCGGTGACCGGGCGCGGGACATTCGGCAACTCGGGCGTACAGCGGCGGTGTCGCG
CGCCTCGATCTCTAACGAAG

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MSTTYAAGEPPRVVSAAARLYAKCLLIGFALLPAYLIAYLWFFSDPHVVFESHAFHEIAIAAATLEGAFVTYVTWVCYRS
SGEPLLRWLTLGFLGFAMIYALHGAFTGMAHHNIWLFLLYGPASRLVMSILLLTGLLSYSRPPDRIERRTSVRTWLPWIV
FFVIVNVVVAYIAYSPVAGALGTRLSMEGGALVFSLLNVAVLLARRIRSPLMVIYGVSIMAFALSSLAFILGKPWNHMWW
LAHAIFAGGFFLLSYGVVQALQTTRSFSAIYSQEDLMSRLSESMARTESALQELRRTNQKLEYMAATDPMTGASNRRQFI
AQVEREMVRAERDGTPFCLLALDLDNFKNINDAYGHQIGDEVLRGVVRQCLDAIRPAGGLARVGGEEFMALLPDMPLEGA
RMTAERVRSSIASSPFGLDFKRVQVTVSVGVAQYGADGSTVDALLRVVDERLYQAKREGRNRVVSR

Sequences:

>Translated_466_residues
MSTTYAAGEPPRVVSAAARLYAKCLLIGFALLPAYLIAYLWFFSDPHVVFESHAFHEIAIAAATLEGAFVTYVTWVCYRS
SGEPLLRWLTLGFLGFAMIYALHGAFTGMAHHNIWLFLLYGPASRLVMSILLLTGLLSYSRPPDRIERRTSVRTWLPWIV
FFVIVNVVVAYIAYSPVAGALGTRLSMEGGALVFSLLNVAVLLARRIRSPLMVIYGVSIMAFALSSLAFILGKPWNHMWW
LAHAIFAGGFFLLSYGVVQALQTTRSFSAIYSQEDLMSRLSESMARTESALQELRRTNQKLEYMAATDPMTGASNRRQFI
AQVEREMVRAERDGTPFCLLALDLDNFKNINDAYGHQIGDEVLRGVVRQCLDAIRPAGGLARVGGEEFMALLPDMPLEGA
RMTAERVRSSIASSPFGLDFKRVQVTVSVGVAQYGADGSTVDALLRVVDERLYQAKREGRNRVVSR
>Mature_465_residues
STTYAAGEPPRVVSAAARLYAKCLLIGFALLPAYLIAYLWFFSDPHVVFESHAFHEIAIAAATLEGAFVTYVTWVCYRSS
GEPLLRWLTLGFLGFAMIYALHGAFTGMAHHNIWLFLLYGPASRLVMSILLLTGLLSYSRPPDRIERRTSVRTWLPWIVF
FVIVNVVVAYIAYSPVAGALGTRLSMEGGALVFSLLNVAVLLARRIRSPLMVIYGVSIMAFALSSLAFILGKPWNHMWWL
AHAIFAGGFFLLSYGVVQALQTTRSFSAIYSQEDLMSRLSESMARTESALQELRRTNQKLEYMAATDPMTGASNRRQFIA
QVEREMVRAERDGTPFCLLALDLDNFKNINDAYGHQIGDEVLRGVVRQCLDAIRPAGGLARVGGEEFMALLPDMPLEGAR
MTAERVRSSIASSPFGLDFKRVQVTVSVGVAQYGADGSTVDALLRVVDERLYQAKREGRNRVVSR

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=168, Percent_Identity=36.9047619047619, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI87081881, Length=176, Percent_Identity=42.0454545454545, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI145693134, Length=206, Percent_Identity=31.5533980582524, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1786584, Length=178, Percent_Identity=33.1460674157303, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1787541, Length=172, Percent_Identity=34.3023255813954, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1788085, Length=182, Percent_Identity=31.3186813186813, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788381, Length=172, Percent_Identity=31.9767441860465, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787802, Length=129, Percent_Identity=38.7596899224806, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87082007, Length=168, Percent_Identity=35.1190476190476, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788956, Length=264, Percent_Identity=28.7878787878788, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787056, Length=158, Percent_Identity=32.9113924050633, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI87081974, Length=168, Percent_Identity=27.3809523809524, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787816, Length=157, Percent_Identity=29.2993630573248, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87081977, Length=178, Percent_Identity=28.0898876404494, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 51706; Mature: 51575

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTYAAGEPPRVVSAAARLYAKCLLIGFALLPAYLIAYLWFFSDPHVVFESHAFHEIAI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHH
AAATLEGAFVTYVTWVCYRSSGEPLLRWLTLGFLGFAMIYALHGAFTGMAHHNIWLFLLY
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
GPASRLVMSILLLTGLLSYSRPPDRIERRTSVRTWLPWIVFFVIVNVVVAYIAYSPVAGA
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGTRLSMEGGALVFSLLNVAVLLARRIRSPLMVIYGVSIMAFALSSLAFILGKPWNHMWW
HHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LAHAIFAGGFFLLSYGVVQALQTTRSFSAIYSQEDLMSRLSESMARTESALQELRRTNQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEYMAATDPMTGASNRRQFIAQVEREMVRAERDGTPFCLLALDLDNFKNINDAYGHQIGD
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHH
EVLRGVVRQCLDAIRPAGGLARVGGEEFMALLPDMPLEGARMTAERVRSSIASSPFGLDF
HHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
KRVQVTVSVGVAQYGADGSTVDALLRVVDERLYQAKREGRNRVVSR
EEEEEEEEECHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
STTYAAGEPPRVVSAAARLYAKCLLIGFALLPAYLIAYLWFFSDPHVVFESHAFHEIAI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHH
AAATLEGAFVTYVTWVCYRSSGEPLLRWLTLGFLGFAMIYALHGAFTGMAHHNIWLFLLY
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
GPASRLVMSILLLTGLLSYSRPPDRIERRTSVRTWLPWIVFFVIVNVVVAYIAYSPVAGA
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGTRLSMEGGALVFSLLNVAVLLARRIRSPLMVIYGVSIMAFALSSLAFILGKPWNHMWW
HHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LAHAIFAGGFFLLSYGVVQALQTTRSFSAIYSQEDLMSRLSESMARTESALQELRRTNQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEYMAATDPMTGASNRRQFIAQVEREMVRAERDGTPFCLLALDLDNFKNINDAYGHQIGD
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHH
EVLRGVVRQCLDAIRPAGGLARVGGEEFMALLPDMPLEGARMTAERVRSSIASSPFGLDF
HHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
KRVQVTVSVGVAQYGADGSTVDALLRVVDERLYQAKREGRNRVVSR
EEEEEEEEECHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA