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The map label for this gene is ansA [H]

Identifier: 78066651

GI number: 78066651

Start: 2228426

End: 2229448

Strand: Reverse

Name: ansA [H]

Synonym: Bcep18194_A5182

Alternate gene names: 78066651

Gene position: 2229448-2228426 (Counterclockwise)

Preceding gene: 78066655

Following gene: 78066646

Centisome position: 60.35

GC content: 70.77

Gene sequence:

>1023_bases
ATGAATACTCCGAATTCCGCATCTCCTTCGTCCGCGGCGGCCCTGCCGCGCATCGCCGTGCTCGCTACCGGCGGCACGAT
CGCCGGCGCCGCACCCGACGCGGCCAGCACGGCCGGCTACCAGGCCGGTGCGCTCGGCGTCAATTTCCTGGTCGACGCGG
TGCCGGCGCTCGCGTCCGTCGCGCGCATCGACGCCGAGCAGATCGCCAGCATCGACAGCAAGGATCTCGCGCTGCCGCTG
TGGAATGCGCTCGCCGCGCGGATCGACGCGCTGATGGCCGATCCTGCGGTCGACGGCATCGTGATCACCCACGGCACCGA
CACGCTCGAGGAAACCGCGTATGCGCTGCATCTGGTCGTTAATGGCGACAAGCCGGTCGTGCTGACGGCCGCGATGCGCC
CGGCGACCGCGCTGTCGTCCGACGGCCCGCTGAACCTGCTGAACGCGGTGACGGTGGCTGCGCATCCGTCCGCGCGCGGG
CAGGGCGTGCTGGTCGCGTTCAACAACCGGATCCACGGCGCGCGCGACGTCGTGAAGACGAGCACCTACGCGGTCGATGC
ATTCCAGTCGCCGGAGCTCGGCGCGCTCGGCTGGGTGCAGGACGGCCGGGTCGAATTCGCACGCCGCGTGACGCGTTCGC
GCGACACGCAACTGGCGATCGCCGCCGTGTGGCCGCCCGTCGAAGTCGTCGCGAGCTACGCGGGCGTGACGCGTACGGCC
GTCGATGCGCTCGTTGCAGCCGGCGTGCGCGGTCTCGTCGTCGCGGGCACCGGCAACGGCTCGATCCATGCGACGCTGCA
GGCGGCGCTGGCCGATGCGGTAAACGCAGGCGTTGCGGTGGTTCGCGCGTCGCGCGTCGGGTCGGGGCACGTGATGCGCA
ACGGCGCCGCGAGCGACGATGCGCTCGGCTTCGTGAGCGCGGGGTCGCTGAACCCGTACAAGGCGCGCGTGCTGCTGATG
CTCGCGCTCGCGAACGGCATCGACGGTCGCGACGCGCTGCAACGCATGTTCGATACGCTGTAA

Upstream 100 bases:

>100_bases
CGGCCGAACGATCGGGACAAACCATGATGTGCGGCGGCCAGCCCCGATCGCCGCGCCGGAACCTGCCGGATCCCGGCCGC
AAGCCTTTACAATCGCGGCC

Downstream 100 bases:

>100_bases
CCGGCTGACCGATACGTGCGGCGCGGGCCGCGCGCGTCACCATGAAAAAAAGGCAGGCCATCGGCCTGCCTTTTTTGCGT
CCGTCAGTCCGGCAACAATG

Product: L-asparaginase, type II

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MNTPNSASPSSAAALPRIAVLATGGTIAGAAPDAASTAGYQAGALGVNFLVDAVPALASVARIDAEQIASIDSKDLALPL
WNALAARIDALMADPAVDGIVITHGTDTLEETAYALHLVVNGDKPVVLTAAMRPATALSSDGPLNLLNAVTVAAHPSARG
QGVLVAFNNRIHGARDVVKTSTYAVDAFQSPELGALGWVQDGRVEFARRVTRSRDTQLAIAAVWPPVEVVASYAGVTRTA
VDALVAAGVRGLVVAGTGNGSIHATLQAALADAVNAGVAVVRASRVGSGHVMRNGAASDDALGFVSAGSLNPYKARVLLM
LALANGIDGRDALQRMFDTL

Sequences:

>Translated_340_residues
MNTPNSASPSSAAALPRIAVLATGGTIAGAAPDAASTAGYQAGALGVNFLVDAVPALASVARIDAEQIASIDSKDLALPL
WNALAARIDALMADPAVDGIVITHGTDTLEETAYALHLVVNGDKPVVLTAAMRPATALSSDGPLNLLNAVTVAAHPSARG
QGVLVAFNNRIHGARDVVKTSTYAVDAFQSPELGALGWVQDGRVEFARRVTRSRDTQLAIAAVWPPVEVVASYAGVTRTA
VDALVAAGVRGLVVAGTGNGSIHATLQAALADAVNAGVAVVRASRVGSGHVMRNGAASDDALGFVSAGSLNPYKARVLLM
LALANGIDGRDALQRMFDTL
>Mature_340_residues
MNTPNSASPSSAAALPRIAVLATGGTIAGAAPDAASTAGYQAGALGVNFLVDAVPALASVARIDAEQIASIDSKDLALPL
WNALAARIDALMADPAVDGIVITHGTDTLEETAYALHLVVNGDKPVVLTAAMRPATALSSDGPLNLLNAVTVAAHPSARG
QGVLVAFNNRIHGARDVVKTSTYAVDAFQSPELGALGWVQDGRVEFARRVTRSRDTQLAIAAVWPPVEVVASYAGVTRTA
VDALVAAGVRGLVVAGTGNGSIHATLQAALADAVNAGVAVVRASRVGSGHVMRNGAASDDALGFVSAGSLNPYKARVLLM
LALANGIDGRDALQRMFDTL

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=230, Percent_Identity=28.695652173913, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789327, Length=331, Percent_Identity=44.1087613293051, Blast_Score=245, Evalue=3e-66,
Organism=Escherichia coli, GI1788065, Length=245, Percent_Identity=33.0612244897959, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17558280, Length=234, Percent_Identity=30.7692307692308, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17558278, Length=234, Percent_Identity=30.7692307692308, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI193207372, Length=234, Percent_Identity=30.7692307692308, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI193207374, Length=234, Percent_Identity=30.7692307692308, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323189, Length=340, Percent_Identity=37.0588235294118, Blast_Score=192, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6323187, Length=340, Percent_Identity=37.0588235294118, Blast_Score=192, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6323186, Length=340, Percent_Identity=37.0588235294118, Blast_Score=192, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6323184, Length=340, Percent_Identity=37.0588235294118, Blast_Score=192, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6320527, Length=323, Percent_Identity=33.1269349845201, Blast_Score=175, Evalue=8e-45,
Organism=Drosophila melanogaster, GI24645530, Length=228, Percent_Identity=28.9473684210526, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24639609, Length=247, Percent_Identity=26.3157894736842, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 34446; Mature: 34446

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTPNSASPSSAAALPRIAVLATGGTIAGAAPDAASTAGYQAGALGVNFLVDAVPALASV
CCCCCCCCCCHHHHCCEEEEEEECCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ARIDAEQIASIDSKDLALPLWNALAARIDALMADPAVDGIVITHGTDTLEETAYALHLVV
HHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHCEEEEEEEE
NGDKPVVLTAAMRPATALSSDGPLNLLNAVTVAAHPSARGQGVLVAFNNRIHGARDVVKT
CCCCCEEEEECCCCCHHCCCCCCHHHHHHEEEEECCCCCCCEEEEEECCCCCCHHHHHHH
STYAVDAFQSPELGALGWVQDGRVEFARRVTRSRDTQLAIAAVWPPVEVVASYAGVTRTA
CCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCHHHHH
VDALVAAGVRGLVVAGTGNGSIHATLQAALADAVNAGVAVVRASRVGSGHVMRNGAASDD
HHHHHHCCCCEEEEEECCCCCEEHHHHHHHHHHHHCCEEEEEECCCCCCCEEECCCCCCC
ALGFVSAGSLNPYKARVLLMLALANGIDGRDALQRMFDTL
CCEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNTPNSASPSSAAALPRIAVLATGGTIAGAAPDAASTAGYQAGALGVNFLVDAVPALASV
CCCCCCCCCCHHHHCCEEEEEEECCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ARIDAEQIASIDSKDLALPLWNALAARIDALMADPAVDGIVITHGTDTLEETAYALHLVV
HHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHCEEEEEEEE
NGDKPVVLTAAMRPATALSSDGPLNLLNAVTVAAHPSARGQGVLVAFNNRIHGARDVVKT
CCCCCEEEEECCCCCHHCCCCCCHHHHHHEEEEECCCCCCCEEEEEECCCCCCHHHHHHH
STYAVDAFQSPELGALGWVQDGRVEFARRVTRSRDTQLAIAAVWPPVEVVASYAGVTRTA
CCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCHHHHH
VDALVAAGVRGLVVAGTGNGSIHATLQAALADAVNAGVAVVRASRVGSGHVMRNGAASDD
HHHHHHCCCCEEEEEECCCCCEEHHHHHHHHHHHHCCEEEEEECCCCCCCEEECCCCCCC
ALGFVSAGSLNPYKARVLLMLALANGIDGRDALQRMFDTL
CCEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8898907; 14500908 [H]