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The map label for this gene is dmlR [H]

Identifier: 78066646

GI number: 78066646

Start: 2222740

End: 2223651

Strand: Reverse

Name: dmlR [H]

Synonym: Bcep18194_A5177

Alternate gene names: 78066646

Gene position: 2223651-2222740 (Counterclockwise)

Preceding gene: 78066651

Following gene: 78066645

Centisome position: 60.19

GC content: 69.52

Gene sequence:

>912_bases
ATGGATCGCTTCAAGCAGATCGAGACGTTCGTGCGGGTGGCCGATGCCGGCAGCCTCGCGGCGGCGGCGCTCGAGGAGGG
CGTGTCGCCGGTGGTGCTCGGGCGGCGCATCGACGCGCTCGAAAAGCGTCTTGGCGTGAAGCTGATGTACCGCTCGACGC
GGCGGCTGGTGGTCAGCGAGGAGGGCGCCGCGTTCCTCGAGCGCTGCCGCGGCCTCCTGTCCGAATGGGACCAGGCCGAG
AACGAGCTGGCCGCCGGGCGGCGGGCGGTCAGCGGGCACCTGATCGTGTCCGCACCGGCTGCGTTCGGGCGCAAGCACGT
CGCGCCGCACGCCCCCGGCTTTCTGGTCGACAAGCCCGAGATGCAGCTGTCGTTCAACCTGACCGACCGCGTCGTCGATC
TCGTGCGCGAGGGCTACGACCTGTCGATCCGGATCGGCGGGGCCGTCGATCCGAACTTCGTCGCGGTGAAGCTCGCGTCG
AACCGGCGAGTCGTATGCGGCACGCCCGAGTATTTCCGCCGGCACGGCCGGCCGAAGTCGCTCGACGATCTGCTCAAGCA
CAACTGCCTGGCGTTCAACCTGCAGGGCGGGCAGAACCGCGGCTGGTATTTCCAGCGCCACGGCAAGATCGCGACGATGC
GCGTGGCCGGCAATCTCGACTGCAACGACGGCGAGCTGCTGCACCGCTGGGTCGCCGAGGGTCTCGGGCTCGGCTGGCGC
TCGACGTGGGAAATCGCCGCGCAGCTCGAGACGGGCGCGCTCGAGACCGTGCTCGACGAGTATGCGCTGCCCGACTACGA
CATCCTCGCGGTCTACCCGCAGCAGCGCTACGTGCCGGCGCGCGTGCGCTACTTCATCGACTACCTGCGCGCCGCTTACG
CGAGCCCCGGCTACTGGAGCACCACGCCGTAA

Upstream 100 bases:

>100_bases
TGATAAGATCAGCCGGAGTCGCTTTTTTCGAAACTAAAAGTATGGAATGACGGCCGGGCGGGGCTTTCCGCCGGTGCCGG
AACAGAGACGGAGACAGGAG

Downstream 100 bases:

>100_bases
CCGGCGCGGACGGCCCGGCCACCGGGCTGTTCGACGATTTATTTACGCTTCGGGCGCGCGGCGCGGGAATAAACTCGACG
CAACGACGGTAAGGTCATCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MDRFKQIETFVRVADAGSLAAAALEEGVSPVVLGRRIDALEKRLGVKLMYRSTRRLVVSEEGAAFLERCRGLLSEWDQAE
NELAAGRRAVSGHLIVSAPAAFGRKHVAPHAPGFLVDKPEMQLSFNLTDRVVDLVREGYDLSIRIGGAVDPNFVAVKLAS
NRRVVCGTPEYFRRHGRPKSLDDLLKHNCLAFNLQGGQNRGWYFQRHGKIATMRVAGNLDCNDGELLHRWVAEGLGLGWR
STWEIAAQLETGALETVLDEYALPDYDILAVYPQQRYVPARVRYFIDYLRAAYASPGYWSTTP

Sequences:

>Translated_303_residues
MDRFKQIETFVRVADAGSLAAAALEEGVSPVVLGRRIDALEKRLGVKLMYRSTRRLVVSEEGAAFLERCRGLLSEWDQAE
NELAAGRRAVSGHLIVSAPAAFGRKHVAPHAPGFLVDKPEMQLSFNLTDRVVDLVREGYDLSIRIGGAVDPNFVAVKLAS
NRRVVCGTPEYFRRHGRPKSLDDLLKHNCLAFNLQGGQNRGWYFQRHGKIATMRVAGNLDCNDGELLHRWVAEGLGLGWR
STWEIAAQLETGALETVLDEYALPDYDILAVYPQQRYVPARVRYFIDYLRAAYASPGYWSTTP
>Mature_303_residues
MDRFKQIETFVRVADAGSLAAAALEEGVSPVVLGRRIDALEKRLGVKLMYRSTRRLVVSEEGAAFLERCRGLLSEWDQAE
NELAAGRRAVSGHLIVSAPAAFGRKHVAPHAPGFLVDKPEMQLSFNLTDRVVDLVREGYDLSIRIGGAVDPNFVAVKLAS
NRRVVCGTPEYFRRHGRPKSLDDLLKHNCLAFNLQGGQNRGWYFQRHGKIATMRVAGNLDCNDGELLHRWVAEGLGLGWR
STWEIAAQLETGALETVLDEYALPDYDILAVYPQQRYVPARVRYFIDYLRAAYASPGYWSTTP

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=291, Percent_Identity=36.426116838488, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1789440, Length=287, Percent_Identity=31.3588850174216, Blast_Score=174, Evalue=5e-45,
Organism=Escherichia coli, GI1787128, Length=284, Percent_Identity=30.6338028169014, Blast_Score=147, Evalue=8e-37,
Organism=Escherichia coli, GI1786401, Length=286, Percent_Identity=29.7202797202797, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=29.0102389078498, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1787589, Length=286, Percent_Identity=27.2727272727273, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1789639, Length=297, Percent_Identity=25.2525252525253, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1790011, Length=296, Percent_Identity=20.6081081081081, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33875; Mature: 33875

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRFKQIETFVRVADAGSLAAAALEEGVSPVVLGRRIDALEKRLGVKLMYRSTRRLVVSE
CCHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHCEEEEEECCCEEEEEC
EGAAFLERCRGLLSEWDQAENELAAGRRAVSGHLIVSAPAAFGRKHVAPHAPGFLVDKPE
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEECCHHHCCCCCCCCCCCCEECCCC
MQLSFNLTDRVVDLVREGYDLSIRIGGAVDPNFVAVKLASNRRVVCGTPEYFRRHGRPKS
CEEEECHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCCEEEECCHHHHHHCCCCCC
LDDLLKHNCLAFNLQGGQNRGWYFQRHGKIATMRVAGNLDCNDGELLHRWVAEGLGLGWR
HHHHHHCCEEEEEECCCCCCCEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCC
STWEIAAQLETGALETVLDEYALPDYDILAVYPQQRYVPARVRYFIDYLRAAYASPGYWS
CHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCC
TTP
CCC
>Mature Secondary Structure
MDRFKQIETFVRVADAGSLAAAALEEGVSPVVLGRRIDALEKRLGVKLMYRSTRRLVVSE
CCHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHCEEEEEECCCEEEEEC
EGAAFLERCRGLLSEWDQAENELAAGRRAVSGHLIVSAPAAFGRKHVAPHAPGFLVDKPE
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEECCHHHCCCCCCCCCCCCEECCCC
MQLSFNLTDRVVDLVREGYDLSIRIGGAVDPNFVAVKLASNRRVVCGTPEYFRRHGRPKS
CEEEECHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCCEEEECCHHHHHHCCCCCC
LDDLLKHNCLAFNLQGGQNRGWYFQRHGKIATMRVAGNLDCNDGELLHRWVAEGLGLGWR
HHHHHHCCEEEEEECCCCCCCEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCC
STWEIAAQLETGALETVLDEYALPDYDILAVYPQQRYVPARVRYFIDYLRAAYASPGYWS
CHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCC
TTP
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]