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The map label for this gene is pcm [H]

Identifier: 78066592

GI number: 78066592

Start: 2166413

End: 2167345

Strand: Reverse

Name: pcm [H]

Synonym: Bcep18194_A5123

Alternate gene names: 78066592

Gene position: 2167345-2166413 (Counterclockwise)

Preceding gene: 78066593

Following gene: 78066591

Centisome position: 58.67

GC content: 70.1

Gene sequence:

>933_bases
ATGAGCGGCGAGCGCGCGAAGCGGTTCCCGCTCGCGCTCGAAGATCTCAAGCGAGCGCCACGCAAGTCGGAAGGTCGGGC
CGGCGAACGCCATGCGGCGGTCGCGGGTTCGAAGGCCGCCGACAAGCCCGCGGCCGTGCTGAAGCCGGTTGCGGTGAAGC
CTGCTGCGGTGCGTACGGCATTGCCCGGTTCCGCCGCCGCGAAGCCGGCGACCGCACCGAAGCCGACCGCGCTGAAGCCC
GCGATGCCGAAGCCGGCCGCGCCGAGCGTCGCGCCGGCCGGCGCGTTTGCGCTCACGTCGGAACGTGTGCGCGAACGGAT
GGTCGAACGGCTGCGCGCGAACGGTGTGACCGACGCGCGCGTGCTGGAAGCGATGGCCGCGGTGCCGCGACACATGTTCG
TGGATCCGGGGCTCGCGACGCAGGCTTACGAGGATTCGGCGTTGCCGATCGGCCACCAGCAGACCATTTCAAAGCCGTCG
GTCGTCGCGCGCATGATCGAGCTCGCGATGGCCGGCCGCACGCTCGAGCGCGTGCTCGAGATCGGCACGGGCTGCGGCTA
CCAGGCCGCCGTGCTGAGTCACGTGGCACGCGACGTGTATTCGATTGAACGCATCAAGCCGCTCTACGAGCGCGCGAAGC
TGAACCTGCGGCCGCTGCGCGTGCCGAACATCCGTCTGCACTACGGCGACGGGCGTGTCGGCCTGCCGTCCGCGGCCCCG
TTCGACGCGATCGTGATCGCGGCGGCGGGGCTCGACGTGCCGCAGGCGCTGCTCGAGCAGCTTGCGATCGGCGGGCGGCT
CGTCGCGCCGGTCGGCGCGCAGAGCGGGCAGCACCAGGTGCTCACGCTCGTCGAGCGCGTCGCGCACGCGCAATGGCGGG
AGTCACGGCTTGATCGCGTTTTCTTTGTCCCTTTAAAATCCGGAGTAATTTGA

Upstream 100 bases:

>100_bases
CAAACGGTTTCGTGTCGATCACGCCGCTGCAGCTGGACCTCACGCATACGCAGATGCTGCCCGCGACGCGCGAATGGGCG
CGCGCCGGAGGGCGGGCTTC

Downstream 100 bases:

>100_bases
AACCGATGAGTATGTTGCGCGCGATGCAAAACAACCGTTCCAGGGAACCGCTCACGCTCGCCCAGCGCGCGATCTGCGTA
GCTGCGTTCTCCACGCTTCT

Product: protein-L-isoaspartate(D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MSGERAKRFPLALEDLKRAPRKSEGRAGERHAAVAGSKAADKPAAVLKPVAVKPAAVRTALPGSAAAKPATAPKPTALKP
AMPKPAAPSVAPAGAFALTSERVRERMVERLRANGVTDARVLEAMAAVPRHMFVDPGLATQAYEDSALPIGHQQTISKPS
VVARMIELAMAGRTLERVLEIGTGCGYQAAVLSHVARDVYSIERIKPLYERAKLNLRPLRVPNIRLHYGDGRVGLPSAAP
FDAIVIAAAGLDVPQALLEQLAIGGRLVAPVGAQSGQHQVLTLVERVAHAQWRESRLDRVFFVPLKSGVI

Sequences:

>Translated_310_residues
MSGERAKRFPLALEDLKRAPRKSEGRAGERHAAVAGSKAADKPAAVLKPVAVKPAAVRTALPGSAAAKPATAPKPTALKP
AMPKPAAPSVAPAGAFALTSERVRERMVERLRANGVTDARVLEAMAAVPRHMFVDPGLATQAYEDSALPIGHQQTISKPS
VVARMIELAMAGRTLERVLEIGTGCGYQAAVLSHVARDVYSIERIKPLYERAKLNLRPLRVPNIRLHYGDGRVGLPSAAP
FDAIVIAAAGLDVPQALLEQLAIGGRLVAPVGAQSGQHQVLTLVERVAHAQWRESRLDRVFFVPLKSGVI
>Mature_309_residues
SGERAKRFPLALEDLKRAPRKSEGRAGERHAAVAGSKAADKPAAVLKPVAVKPAAVRTALPGSAAAKPATAPKPTALKPA
MPKPAAPSVAPAGAFALTSERVRERMVERLRANGVTDARVLEAMAAVPRHMFVDPGLATQAYEDSALPIGHQQTISKPSV
VARMIELAMAGRTLERVLEIGTGCGYQAAVLSHVARDVYSIERIKPLYERAKLNLRPLRVPNIRLHYGDGRVGLPSAAPF
DAIVIAAAGLDVPQALLEQLAIGGRLVAPVGAQSGQHQVLTLVERVAHAQWRESRLDRVFFVPLKSGVI

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI226530908, Length=214, Percent_Identity=34.1121495327103, Blast_Score=91, Evalue=9e-19,
Organism=Escherichia coli, GI1789100, Length=211, Percent_Identity=45.4976303317536, Blast_Score=162, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI71983477, Length=223, Percent_Identity=31.8385650224215, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI193207222, Length=219, Percent_Identity=31.0502283105023, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17981723, Length=218, Percent_Identity=32.5688073394495, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 32927; Mature: 32796

Theoretical pI: Translated: 11.04; Mature: 11.04

Prosite motif: PS01279 PCMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGERAKRFPLALEDLKRAPRKSEGRAGERHAAVAGSKAADKPAAVLKPVAVKPAAVRTA
CCCCCCHHCCCHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHCCCCCCCHHHHHC
LPGSAAAKPATAPKPTALKPAMPKPAAPSVAPAGAFALTSERVRERMVERLRANGVTDAR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
VLEAMAAVPRHMFVDPGLATQAYEDSALPIGHQQTISKPSVVARMIELAMAGRTLERVLE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHH
IGTGCGYQAAVLSHVARDVYSIERIKPLYERAKLNLRPLRVPNIRLHYGDGRVGLPSAAP
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCEEEEEECCCCCCCCCCCC
FDAIVIAAAGLDVPQALLEQLAIGGRLVAPVGAQSGQHQVLTLVERVAHAQWRESRLDRV
HHHHHHHHCCCCHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCEE
FFVPLKSGVI
EEEECCCCCC
>Mature Secondary Structure 
SGERAKRFPLALEDLKRAPRKSEGRAGERHAAVAGSKAADKPAAVLKPVAVKPAAVRTA
CCCCCHHCCCHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHCCCCCCCHHHHHC
LPGSAAAKPATAPKPTALKPAMPKPAAPSVAPAGAFALTSERVRERMVERLRANGVTDAR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
VLEAMAAVPRHMFVDPGLATQAYEDSALPIGHQQTISKPSVVARMIELAMAGRTLERVLE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHH
IGTGCGYQAAVLSHVARDVYSIERIKPLYERAKLNLRPLRVPNIRLHYGDGRVGLPSAAP
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCEEEEEECCCCCCCCCCCC
FDAIVIAAAGLDVPQALLEQLAIGGRLVAPVGAQSGQHQVLTLVERVAHAQWRESRLDRV
HHHHHHHHCCCCHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCEE
FFVPLKSGVI
EEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA