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The map label for this gene is qor [C]

Identifier: 78066544

GI number: 78066544

Start: 2113207

End: 2114223

Strand: Reverse

Name: qor [C]

Synonym: Bcep18194_A5075

Alternate gene names: 78066544

Gene position: 2114223-2113207 (Counterclockwise)

Preceding gene: 78066547

Following gene: 219380069

Centisome position: 57.23

GC content: 68.53

Gene sequence:

>1017_bases
ATGAAAGCCGTTGGACTGACCCGTTATCTGCCGATCGACGACCCGCAAGCGCTGCTCGACGTGGAGCTGCCGCAGCCTGT
GCCGGGCCCGCGCGACCTGCTCGTGAAGGTCGAGGCGATTTCCGTGAACCCGGTGGACTTCAAGGTGCGCGCGCCGAAGC
CGCAGGTCGAGGACACGCCGCGCGTGCTCGGCTGGGATGCGGCCGGCACGGTGGTCGCGGTCGGCGCCGACGTGACGCTG
TTCCGGCCCGGCGACGAGGTGTTCTACGCGGGCAGCATCACGCGGCCGGGTGCGAACAGCGAATTTCATACGGTCGACGA
GCGGATCGCCGCGCTGAAGCCGCGCACGCTCGATTTCGCCGCCGCCGCCGCGCTGCCGCTCACGGCGCTGACCGCGTGGG
AAGCGCTGTTCGACCGGCTGCACGTGTCGCCGCAGGGCGCGGACGCCGGCAAGTCGGTGCTGATCATCGGCGGCGCGGGT
GGCGTGGGCTCGATCGCGATCCAGCTCGCGAAGACGCTCGGCAAGCTGCACGTGATCGCCACCGCGTCGCGGCCGGCGTC
GGCCGACTGGGTGCGCTCGCTGGGTGCCGACGCGGTGGTCGACCATTTCGGCGACCTGCCTGCGCAGTTGCGCGAGGCCG
GGCATCCGAACGTCGACTACGTGCTGATTTTCAACGACACGGATCGTCACTTTCCTGCCGCAGCGGAGGTAATCCGGCCG
CAGGGCGGCATTTGCACGATCGTCGAGAACGACAAGCCGGTGCCGGTCGAACTGCTGAAGGCGAAGAGTGCCGCGTTTCA
CTGGGAATTCATGTTTACGCGTGCAATGTTCGAGACGCCGGACATGATCGAGCAGCACCGGATCCTCGGCGAAGTCGCGC
GGCTCGTCGATGGCGGCACGCTGCGCACCACGCTCGGCGAGCAGCTCGGCACGATCAATGCCGCGAACGTGCGGCGCGCG
CACCAGTTGCTCGAGGCCGGGCGTTCGATCGGCAAGCTCGTGCTGAGCGGCTTCTGA

Upstream 100 bases:

>100_bases
TTTCAGGAAATATAGCGGTTTTCTCGAATGATCGGGTTGCTTACGATGACGTTCATGGGGCTGCGCCGCGGCCCACAGGA
TTTCCTGAAGGAGTGTCGTG

Downstream 100 bases:

>100_bases
GCCGGCATCGCGCGTAAGGTTGCGGTGCCGCGAACGCAAGCGGGGTAACACCCGATGCGTTTGCGGCACATTGCATGCCG
CTTGGCGGTAGACTGGCAGC

Product: zinc-containing alcohol dehydrogenase superfamily protein

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MKAVGLTRYLPIDDPQALLDVELPQPVPGPRDLLVKVEAISVNPVDFKVRAPKPQVEDTPRVLGWDAAGTVVAVGADVTL
FRPGDEVFYAGSITRPGANSEFHTVDERIAALKPRTLDFAAAAALPLTALTAWEALFDRLHVSPQGADAGKSVLIIGGAG
GVGSIAIQLAKTLGKLHVIATASRPASADWVRSLGADAVVDHFGDLPAQLREAGHPNVDYVLIFNDTDRHFPAAAEVIRP
QGGICTIVENDKPVPVELLKAKSAAFHWEFMFTRAMFETPDMIEQHRILGEVARLVDGGTLRTTLGEQLGTINAANVRRA
HQLLEAGRSIGKLVLSGF

Sequences:

>Translated_338_residues
MKAVGLTRYLPIDDPQALLDVELPQPVPGPRDLLVKVEAISVNPVDFKVRAPKPQVEDTPRVLGWDAAGTVVAVGADVTL
FRPGDEVFYAGSITRPGANSEFHTVDERIAALKPRTLDFAAAAALPLTALTAWEALFDRLHVSPQGADAGKSVLIIGGAG
GVGSIAIQLAKTLGKLHVIATASRPASADWVRSLGADAVVDHFGDLPAQLREAGHPNVDYVLIFNDTDRHFPAAAEVIRP
QGGICTIVENDKPVPVELLKAKSAAFHWEFMFTRAMFETPDMIEQHRILGEVARLVDGGTLRTTLGEQLGTINAANVRRA
HQLLEAGRSIGKLVLSGF
>Mature_338_residues
MKAVGLTRYLPIDDPQALLDVELPQPVPGPRDLLVKVEAISVNPVDFKVRAPKPQVEDTPRVLGWDAAGTVVAVGADVTL
FRPGDEVFYAGSITRPGANSEFHTVDERIAALKPRTLDFAAAAALPLTALTAWEALFDRLHVSPQGADAGKSVLIIGGAG
GVGSIAIQLAKTLGKLHVIATASRPASADWVRSLGADAVVDHFGDLPAQLREAGHPNVDYVLIFNDTDRHFPAAAEVIRP
QGGICTIVENDKPVPVELLKAKSAAFHWEFMFTRAMFETPDMIEQHRILGEVARLVDGGTLRTTLGEQLGTINAANVRRA
HQLLEAGRSIGKLVLSGF

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI47519420, Length=199, Percent_Identity=39.1959798994975, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI194239674, Length=207, Percent_Identity=33.8164251207729, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI13236495, Length=207, Percent_Identity=33.8164251207729, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI194239676, Length=207, Percent_Identity=33.8164251207729, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI28557745, Length=209, Percent_Identity=31.1004784688995, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI18379349, Length=219, Percent_Identity=29.6803652968037, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790485, Length=202, Percent_Identity=33.6633663366337, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71987554, Length=218, Percent_Identity=36.2385321100917, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17507255, Length=193, Percent_Identity=34.1968911917098, Blast_Score=77, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR014182
- InterPro:   IPR011032
- InterPro:   IPR016040
- InterPro:   IPR002364 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 36124; Mature: 36124

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS01162 QOR_ZETA_CRYSTAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVGLTRYLPIDDPQALLDVELPQPVPGPRDLLVKVEAISVNPVDFKVRAPKPQVEDTP
CCCCCCEEECCCCCCCCEEEEECCCCCCCCHHEEEEEEEEEECCCEEEEECCCCCCCCCC
RVLGWDAAGTVVAVGADVTLFRPGDEVFYAGSITRPGANSEFHTVDERIAALKPRTLDFA
CEEEECCCCCEEEECCCEEEECCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCHHHH
AAAALPLTALTAWEALFDRLHVSPQGADAGKSVLIIGGAGGVGSIAIQLAKTLGKLHVIA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEE
TASRPASADWVRSLGADAVVDHFGDLPAQLREAGHPNVDYVLIFNDTDRHFPAAAEVIRP
ECCCCCCHHHHHHCCCHHHHHHHCCCCHHHHHCCCCCCEEEEEECCCCCCCCCHHHHCCC
QGGICTIVENDKPVPVELLKAKSAAFHWEFMFTRAMFETPDMIEQHRILGEVARLVDGGT
CCCEEEEEECCCCCCHHHHHHCCHHEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCC
LRTTLGEQLGTINAANVRRAHQLLEAGRSIGKLVLSGF
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKAVGLTRYLPIDDPQALLDVELPQPVPGPRDLLVKVEAISVNPVDFKVRAPKPQVEDTP
CCCCCCEEECCCCCCCCEEEEECCCCCCCCHHEEEEEEEEEECCCEEEEECCCCCCCCCC
RVLGWDAAGTVVAVGADVTLFRPGDEVFYAGSITRPGANSEFHTVDERIAALKPRTLDFA
CEEEECCCCCEEEECCCEEEECCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCHHHH
AAAALPLTALTAWEALFDRLHVSPQGADAGKSVLIIGGAGGVGSIAIQLAKTLGKLHVIA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEE
TASRPASADWVRSLGADAVVDHFGDLPAQLREAGHPNVDYVLIFNDTDRHFPAAAEVIRP
ECCCCCCHHHHHHCCCHHHHHHHCCCCHHHHHCCCCCCEEEEEECCCCCCCCCHHHHCCC
QGGICTIVENDKPVPVELLKAKSAAFHWEFMFTRAMFETPDMIEQHRILGEVARLVDGGT
CCCEEEEEECCCCCCHHHHHHCCHHEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCC
LRTTLGEQLGTINAANVRRAHQLLEAGRSIGKLVLSGF
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]