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The map label for this gene is potF [H]

Identifier: 78066519

GI number: 78066519

Start: 2087555

End: 2088649

Strand: Reverse

Name: potF [H]

Synonym: Bcep18194_A5050

Alternate gene names: 78066519

Gene position: 2088649-2087555 (Counterclockwise)

Preceding gene: 78066521

Following gene: 78066518

Centisome position: 56.54

GC content: 62.92

Gene sequence:

>1095_bases
ATGAAAGCAAATGTAGTGGGCCGGTTCGCAGCGCTCGCGCTGTGCGTGGGTGCATCGGCGGCAGCGGCGAAGGATACGCA
GCTCAATGTCTATAACTGGTCGGACTACATTGCGAAGGACACGATCCCGAACTTCGAGAAGCAGACGGGCGTCAAGGTCC
GCTACGACAACTACGACAGCGACGACACGCTGCAGGCGAAGCTGCTGACGGGCAGCTCGGGCTACGACATCGTCGTGCCG
ACCAGCAACTACGCGGGCAAGCAGATCGCCGCCGGCATCTTCGCGCCGCTCGACAAGTCGAAGCTGCCGAACCTCAAGTA
CCTCGATCCGCAACTGATGGCGCTCGTCGCGGGTGCCGATCCGGGCAACAAGTTCTCGGTGCCCTGGGCATACGGCACGA
CCGGTCTCGCATACAACCTGACGAAGGCGCAGCAGGCGCTCGGCAAGGTGCCGCTCGACAACTGGGACATCCTGTTCAAG
CCGGAAAACCTCTCGAAGCTGAAGACCTGCGGCGTGTCGGTGCTCGACGCACCCGACCAGATGTTCGCGGCGACGCTGCA
CTACATCGGCAAGGATCCGATGAGCACGAACCCGGCCGACTACAAGGCCGCGATGGAAGTGCTGAAGAAGATCCGCCCGT
ACATCACGCAATTCAACTCGTCGGGCTACATCAACGACCTGGTCGGCGGCGACATCTGCTTCGCATTCGGCTGGTCGGGC
GACGTCGTGATCGCGAAGCATCGCGCGATGGAAGCGAAGAAGCCGTACAAGGTCGAGTACTACATCCCGAAGGGCGGCGC
ACCGGTGTGGTTCGACGTGATGGCGATCCCGAAGGACGCGAAGAACAAGGATGCCGCGCTGCAGTGGATCAACTACATCG
AGGATCCGAAGGTGCACGCGGCGATCACGAACGCGGTGTACTACCCGAGCGCCAACGCCGAGGCCCGCAAGTACGTGCGG
CCCGACGTCGCGAACGACCCGGCCGTCTATCCGCCGGCCGACGTCGTGAAGACGCTGTTCCTGCTCAAGCCGCTGCCGCC
TGAAATCCAGCGCCTGCAGACGCGTCTGTGGACCGAGCTGAAGTCGGGCCGCTGA

Upstream 100 bases:

>100_bases
TGGTAGTTTTCGGGGTTTTCGAGGGGCGGCGTGCCAGAATGCGCCGCCATGACGAGGCAGACGGCAGCCGGACACACGGT
CCGGTCGGAGGGAGAACGGG

Downstream 100 bases:

>100_bases
CACGCGCCGATGTTCCCAGTAGTCATGAAGCCCCTGGTGCCCCCGGGGGCTTTGTTCGTTCAACGCAGGAGAGAAGCAGC
ACATCATGAATAGCCAGTCG

Product: ABC spermidine/putrescine transporter, periplasmic ligand binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MKANVVGRFAALALCVGASAAAAKDTQLNVYNWSDYIAKDTIPNFEKQTGVKVRYDNYDSDDTLQAKLLTGSSGYDIVVP
TSNYAGKQIAAGIFAPLDKSKLPNLKYLDPQLMALVAGADPGNKFSVPWAYGTTGLAYNLTKAQQALGKVPLDNWDILFK
PENLSKLKTCGVSVLDAPDQMFAATLHYIGKDPMSTNPADYKAAMEVLKKIRPYITQFNSSGYINDLVGGDICFAFGWSG
DVVIAKHRAMEAKKPYKVEYYIPKGGAPVWFDVMAIPKDAKNKDAALQWINYIEDPKVHAAITNAVYYPSANAEARKYVR
PDVANDPAVYPPADVVKTLFLLKPLPPEIQRLQTRLWTELKSGR

Sequences:

>Translated_364_residues
MKANVVGRFAALALCVGASAAAAKDTQLNVYNWSDYIAKDTIPNFEKQTGVKVRYDNYDSDDTLQAKLLTGSSGYDIVVP
TSNYAGKQIAAGIFAPLDKSKLPNLKYLDPQLMALVAGADPGNKFSVPWAYGTTGLAYNLTKAQQALGKVPLDNWDILFK
PENLSKLKTCGVSVLDAPDQMFAATLHYIGKDPMSTNPADYKAAMEVLKKIRPYITQFNSSGYINDLVGGDICFAFGWSG
DVVIAKHRAMEAKKPYKVEYYIPKGGAPVWFDVMAIPKDAKNKDAALQWINYIEDPKVHAAITNAVYYPSANAEARKYVR
PDVANDPAVYPPADVVKTLFLLKPLPPEIQRLQTRLWTELKSGR
>Mature_364_residues
MKANVVGRFAALALCVGASAAAAKDTQLNVYNWSDYIAKDTIPNFEKQTGVKVRYDNYDSDDTLQAKLLTGSSGYDIVVP
TSNYAGKQIAAGIFAPLDKSKLPNLKYLDPQLMALVAGADPGNKFSVPWAYGTTGLAYNLTKAQQALGKVPLDNWDILFK
PENLSKLKTCGVSVLDAPDQMFAATLHYIGKDPMSTNPADYKAAMEVLKKIRPYITQFNSSGYINDLVGGDICFAFGWSG
DVVIAKHRAMEAKKPYKVEYYIPKGGAPVWFDVMAIPKDAKNKDAALQWINYIEDPKVHAAITNAVYYPSANAEARKYVR
PDVANDPAVYPPADVVKTLFLLKPLPPEIQRLQTRLWTELKSGR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=360, Percent_Identity=51.9444444444444, Blast_Score=383, Evalue=1e-108,
Organism=Escherichia coli, GI1787367, Length=321, Percent_Identity=35.5140186915888, Blast_Score=181, Evalue=5e-47,
Organism=Escherichia coli, GI1787711, Length=319, Percent_Identity=23.1974921630094, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40000; Mature: 40000

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKANVVGRFAALALCVGASAAAAKDTQLNVYNWSDYIAKDTIPNFEKQTGVKVRYDNYDS
CCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHCCCCCCHHHCCCEEEECCCCC
DDTLQAKLLTGSSGYDIVVPTSNYAGKQIAAGIFAPLDKSKLPNLKYLDPQLMALVAGAD
CCCEEEEEEECCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEECCC
PGNKFSVPWAYGTTGLAYNLTKAQQALGKVPLDNWDILFKPENLSKLKTCGVSVLDAPDQ
CCCEEECCEECCCCCEEEEHHHHHHHHCCCCCCCCEEEECCCCHHHHHHCCCEEECCCHH
MFAATLHYIGKDPMSTNPADYKAAMEVLKKIRPYITQFNSSGYINDLVGGDICFAFGWSG
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCEEEEECCCC
DVVIAKHRAMEAKKPYKVEYYIPKGGAPVWFDVMAIPKDAKNKDAALQWINYIEDPKVHA
CEEEEECHHHCCCCCEEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEE
AITNAVYYPSANAEARKYVRPDVANDPAVYPPADVVKTLFLLKPLPPEIQRLQTRLWTEL
EEEEEEECCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
KSGR
HCCC
>Mature Secondary Structure
MKANVVGRFAALALCVGASAAAAKDTQLNVYNWSDYIAKDTIPNFEKQTGVKVRYDNYDS
CCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHCCCCCCHHHCCCEEEECCCCC
DDTLQAKLLTGSSGYDIVVPTSNYAGKQIAAGIFAPLDKSKLPNLKYLDPQLMALVAGAD
CCCEEEEEEECCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEECCC
PGNKFSVPWAYGTTGLAYNLTKAQQALGKVPLDNWDILFKPENLSKLKTCGVSVLDAPDQ
CCCEEECCEECCCCCEEEEHHHHHHHHCCCCCCCCEEEECCCCHHHHHHCCCEEECCCHH
MFAATLHYIGKDPMSTNPADYKAAMEVLKKIRPYITQFNSSGYINDLVGGDICFAFGWSG
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCEEEEECCCC
DVVIAKHRAMEAKKPYKVEYYIPKGGAPVWFDVMAIPKDAKNKDAALQWINYIEDPKVHA
CEEEEECHHHCCCCCEEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEE
AITNAVYYPSANAEARKYVRPDVANDPAVYPPADVVKTLFLLKPLPPEIQRLQTRLWTEL
EEEEEEECCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
KSGR
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]