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The map label for this gene is potI [H]

Identifier: 78066516

GI number: 78066516

Start: 2084568

End: 2085389

Strand: Reverse

Name: potI [H]

Synonym: Bcep18194_A5047

Alternate gene names: 78066516

Gene position: 2085389-2084568 (Counterclockwise)

Preceding gene: 78066517

Following gene: 78066511

Centisome position: 56.45

GC content: 64.36

Gene sequence:

>822_bases
ATGAAGCCGAATCGTTACCTGCAGTTCGCGGCGCTGTTTGCCGGCTTCGCGTTCCTGTACATCCCGATCATCAGCCTGAT
CGTCTATTCGTTCAACGAGTCGAAGCTCGTCACCGTGTGGTCGGGCTTCTCGTTCCGCTGGTACTCGGCGCTCGTGGAAG
ACGACGAGTTGCTGACGGCCGCGTGGCTGTCGCTGAAGATCGGCGTGCTGACCGCGTTCGCGTCGGTGATCATCGGCACG
TGGGCCGGCTTCGTGCTCGCGCGGATGGGACGCTTTCGCGGTTTCGCGCTGTTCAGCGGGATGATCAACGCGCCGCTCGT
GATTCCCGAGGTGATCCAGGGGATCTCGCTGCTGCTGCTGTTCATCGAGCTGGCGAAGTGGATCGGCTGGCCGGCCGAGC
GTGGCGTGTTCACGATCTGGCTGGGTCACGTGATGCTGTGCATCTCGTATGTCGCGATCATCGTGCAGTCGCGCGTGCGC
GAGCTGAACCCGTCGCTGGAAGAAGCCGCGCTCGATCTCGGCGCGACGCCGTTGAAGGTGTTCTTCACGATCACGCTGCC
GCTGATCTCGCAGGCGTTGATCGCGGGCTGGCTGCTGTCGTTCACGCTGTCGATCGACGACCTCGTGCTGTCGGCGTTCC
TGTCGGGCCCCGGCTCGACGACGCTGCCGCTCGTGGTGTTCTCGCGCGTGCGGCTGGGGCTGAACCCGGAGATGAACGCG
CTCGCGACGCTGTTCATCGTCGCGGTGACGGCCGGTGTCGTGATCGCGAACTTCGTGATGCTGCGGCAGGAACGCAAGCG
GATGGCGGGGTTCGCGACCTGA

Upstream 100 bases:

>100_bases
GGCCGATGGCGTCGGCGGTGACCTGCGCGATGGTGCTGCTGCTGCTCGTGCCGATGGCGATGTTCCAGCACTTCCAGGCG
AAAGAGCAGGGAGGCCGTCG

Downstream 100 bases:

>100_bases
CGTTCGGGTTGTGATCGCGGCTGTTAAAAAACGCCCGGTGCCGACGACGAGTCGGGCCGGGCGTTTTGTTTGGCGAGATG
GGCTCGCGAAAGTGCCTGTC

Product: ABC spermidine/putrescine transporter, inner membrane subunit

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MKPNRYLQFAALFAGFAFLYIPIISLIVYSFNESKLVTVWSGFSFRWYSALVEDDELLTAAWLSLKIGVLTAFASVIIGT
WAGFVLARMGRFRGFALFSGMINAPLVIPEVIQGISLLLLFIELAKWIGWPAERGVFTIWLGHVMLCISYVAIIVQSRVR
ELNPSLEEAALDLGATPLKVFFTITLPLISQALIAGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
LATLFIVAVTAGVVIANFVMLRQERKRMAGFAT

Sequences:

>Translated_273_residues
MKPNRYLQFAALFAGFAFLYIPIISLIVYSFNESKLVTVWSGFSFRWYSALVEDDELLTAAWLSLKIGVLTAFASVIIGT
WAGFVLARMGRFRGFALFSGMINAPLVIPEVIQGISLLLLFIELAKWIGWPAERGVFTIWLGHVMLCISYVAIIVQSRVR
ELNPSLEEAALDLGATPLKVFFTITLPLISQALIAGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
LATLFIVAVTAGVVIANFVMLRQERKRMAGFAT
>Mature_273_residues
MKPNRYLQFAALFAGFAFLYIPIISLIVYSFNESKLVTVWSGFSFRWYSALVEDDELLTAAWLSLKIGVLTAFASVIIGT
WAGFVLARMGRFRGFALFSGMINAPLVIPEVIQGISLLLLFIELAKWIGWPAERGVFTIWLGHVMLCISYVAIIVQSRVR
ELNPSLEEAALDLGATPLKVFFTITLPLISQALIAGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
LATLFIVAVTAGVVIANFVMLRQERKRMAGFAT

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=257, Percent_Identity=59.9221789883268, Blast_Score=285, Evalue=2e-78,
Organism=Escherichia coli, GI1787368, Length=243, Percent_Identity=39.0946502057613, Blast_Score=167, Evalue=8e-43,
Organism=Escherichia coli, GI1787714, Length=248, Percent_Identity=32.6612903225806, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788764, Length=213, Percent_Identity=29.5774647887324, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30060; Mature: 30060

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPNRYLQFAALFAGFAFLYIPIISLIVYSFNESKLVTVWSGFSFRWYSALVEDDELLTA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCHHHHH
AWLSLKIGVLTAFASVIIGTWAGFVLARMGRFRGFALFSGMINAPLVIPEVIQGISLLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FIELAKWIGWPAERGVFTIWLGHVMLCISYVAIIVQSRVRELNPSLEEAALDLGATPLKV
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHH
FFTITLPLISQALIAGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHH
LATLFIVAVTAGVVIANFVMLRQERKRMAGFAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKPNRYLQFAALFAGFAFLYIPIISLIVYSFNESKLVTVWSGFSFRWYSALVEDDELLTA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCHHHHH
AWLSLKIGVLTAFASVIIGTWAGFVLARMGRFRGFALFSGMINAPLVIPEVIQGISLLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FIELAKWIGWPAERGVFTIWLGHVMLCISYVAIIVQSRVRELNPSLEEAALDLGATPLKV
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHH
FFTITLPLISQALIAGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHH
LATLFIVAVTAGVVIANFVMLRQERKRMAGFAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]