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The map label for this gene is potH [H]

Identifier: 78066517

GI number: 78066517

Start: 2085386

End: 2086312

Strand: Reverse

Name: potH [H]

Synonym: Bcep18194_A5048

Alternate gene names: 78066517

Gene position: 2086312-2085386 (Counterclockwise)

Preceding gene: 78066518

Following gene: 78066516

Centisome position: 56.48

GC content: 62.68

Gene sequence:

>927_bases
ATGACCACGTTCAAGTCCCTGCTCGAGTGGCCGGTGCGGCGCTTCAACCTGACGGGCGCCACGGCGGTCGTCGCCGGGCC
GTTCACGTGGCTCGTGCTGTTCTTCCTCGTGCCGTTCGTGCTGGTCGTCAAGATCAGTTTCGCGGAGCTGCAGCTCGGCA
TCCCGCCGTACACGGAACTCGCGTCGTACACCGACGGCGTCGTGCACGTCGCGCTGAACCTGTCGCACTACGCGTTCCTG
TTTACGGACAGCCTGTATTTCGCGACCTACGTGAACTCGGTGTGGGTGGCCGCGATCACGACGCTGCTGTGTTTGCTGAT
CGGCTATCCGATGGCGTACTACATCGCGCGCTCGAACCCGGCGACCCGCAACCTGCTGATGATGGGCGTGATGTTGCCGT
TCTGGACGTCGTTCCTGATCCGCGTGTACGCGTGGATCGGCATCCTGAAGAACAACGGGCTGCTGAACAACTTCCTGATG
TGGATCGGCCTCACGCATACGCCGATCGAGCTGTATCGCACCAACTACGCGGTGTACATCGGGATGGTGTATTCGTACCT
GCCGTTCCTCGTGATGCCGCTGTACGCGCACCTCGTGAAGATGGACCTGCGCCTGCTCGAAGCCGCGTACGACCTCGGCG
CCAAGCCGTGGAAGGCGTTCGTGCAGATCACGCTGCCGCTGTCGAAGAACGGGATCATCGCGGGCTGCCTGCTCGTGTTC
ATCCCGGCGGTCGGCGAGTACGTGATCCCCGAGCTGCTCGGCGGCGCGAACACGCTGATGATCGGCCGCGTGATGTGGAA
TGAATTCTTCAACAACGCAGACTGGCCGATGGCGTCGGCGGTGACCTGCGCGATGGTGCTGCTGCTGCTCGTGCCGATGG
CGATGTTCCAGCACTTCCAGGCGAAAGAGCAGGGAGGCCGTCGATGA

Upstream 100 bases:

>100_bases
CGAACGTATCGAGCCTTGCGATTTCGGAGCTCGACACGCCGGCGCTCGGCGACGAGATCTACGTGCGCTGGAGCGCGACC
GCGGGCGTGGTGCTGACGTC

Downstream 100 bases:

>100_bases
AGCCGAATCGTTACCTGCAGTTCGCGGCGCTGTTTGCCGGCTTCGCGTTCCTGTACATCCCGATCATCAGCCTGATCGTC
TATTCGTTCAACGAGTCGAA

Product: ABC spermidine/putrescine transporter, inner membrane subunit

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MTTFKSLLEWPVRRFNLTGATAVVAGPFTWLVLFFLVPFVLVVKISFAELQLGIPPYTELASYTDGVVHVALNLSHYAFL
FTDSLYFATYVNSVWVAAITTLLCLLIGYPMAYYIARSNPATRNLLMMGVMLPFWTSFLIRVYAWIGILKNNGLLNNFLM
WIGLTHTPIELYRTNYAVYIGMVYSYLPFLVMPLYAHLVKMDLRLLEAAYDLGAKPWKAFVQITLPLSKNGIIAGCLLVF
IPAVGEYVIPELLGGANTLMIGRVMWNEFFNNADWPMASAVTCAMVLLLLVPMAMFQHFQAKEQGGRR

Sequences:

>Translated_308_residues
MTTFKSLLEWPVRRFNLTGATAVVAGPFTWLVLFFLVPFVLVVKISFAELQLGIPPYTELASYTDGVVHVALNLSHYAFL
FTDSLYFATYVNSVWVAAITTLLCLLIGYPMAYYIARSNPATRNLLMMGVMLPFWTSFLIRVYAWIGILKNNGLLNNFLM
WIGLTHTPIELYRTNYAVYIGMVYSYLPFLVMPLYAHLVKMDLRLLEAAYDLGAKPWKAFVQITLPLSKNGIIAGCLLVF
IPAVGEYVIPELLGGANTLMIGRVMWNEFFNNADWPMASAVTCAMVLLLLVPMAMFQHFQAKEQGGRR
>Mature_307_residues
TTFKSLLEWPVRRFNLTGATAVVAGPFTWLVLFFLVPFVLVVKISFAELQLGIPPYTELASYTDGVVHVALNLSHYAFLF
TDSLYFATYVNSVWVAAITTLLCLLIGYPMAYYIARSNPATRNLLMMGVMLPFWTSFLIRVYAWIGILKNNGLLNNFLMW
IGLTHTPIELYRTNYAVYIGMVYSYLPFLVMPLYAHLVKMDLRLLEAAYDLGAKPWKAFVQITLPLSKNGIIAGCLLVFI
PAVGEYVIPELLGGANTLMIGRVMWNEFFNNADWPMASAVTCAMVLLLLVPMAMFQHFQAKEQGGRR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=287, Percent_Identity=57.8397212543554, Blast_Score=328, Evalue=4e-91,
Organism=Escherichia coli, GI1787369, Length=266, Percent_Identity=42.4812030075188, Blast_Score=196, Evalue=1e-51,
Organism=Escherichia coli, GI1787713, Length=222, Percent_Identity=28.3783783783784, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34745; Mature: 34614

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFKSLLEWPVRRFNLTGATAVVAGPFTWLVLFFLVPFVLVVKISFAELQLGIPPYTEL
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHH
ASYTDGVVHVALNLSHYAFLFTDSLYFATYVNSVWVAAITTLLCLLIGYPMAYYIARSNP
HHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEECCCC
ATRNLLMMGVMLPFWTSFLIRVYAWIGILKNNGLLNNFLMWIGLTHTPIELYRTNYAVYI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHCCHHHHH
GMVYSYLPFLVMPLYAHLVKMDLRLLEAAYDLGAKPWKAFVQITLPLSKNGIIAGCLLVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHH
IPAVGEYVIPELLGGANTLMIGRVMWNEFFNNADWPMASAVTCAMVLLLLVPMAMFQHFQ
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AKEQGGRR
HHHCCCCC
>Mature Secondary Structure 
TTFKSLLEWPVRRFNLTGATAVVAGPFTWLVLFFLVPFVLVVKISFAELQLGIPPYTEL
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHH
ASYTDGVVHVALNLSHYAFLFTDSLYFATYVNSVWVAAITTLLCLLIGYPMAYYIARSNP
HHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEECCCC
ATRNLLMMGVMLPFWTSFLIRVYAWIGILKNNGLLNNFLMWIGLTHTPIELYRTNYAVYI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHCCHHHHH
GMVYSYLPFLVMPLYAHLVKMDLRLLEAAYDLGAKPWKAFVQITLPLSKNGIIAGCLLVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHH
IPAVGEYVIPELLGGANTLMIGRVMWNEFFNNADWPMASAVTCAMVLLLLVPMAMFQHFQ
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AKEQGGRR
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]