Click here to switch to the map view.

The map label for this gene is ycjG [H]

Identifier: 78065822

GI number: 78065822

Start: 1306579

End: 1307628

Strand: Reverse

Name: ycjG [H]

Synonym: Bcep18194_A4351

Alternate gene names: 78065822

Gene position: 1307628-1306579 (Counterclockwise)

Preceding gene: 78065823

Following gene: 78065821

Centisome position: 35.4

GC content: 65.33

Gene sequence:

>1050_bases
ATGCGTAATTCCCTTCTTCCTCACTCTTCCATGTCACCCCGCACGCTCTCACTCTCGCTGCGTATCGAACGCTGGCCGTT
GGCCGCTCCGTTCCATATCACCGGCCACGTCATCGACGCCGCTCGCCTCGTGTGCGTGACCGTCGACGACGGGCACCACG
CCGGCAACGGCGAAGCATCCGGGGTCTTCTACCTGGGTGAGACACCCGAATCCATCGTCGCGCAAATCGACCGCGCCCGA
CCATCGATAGAAGCGGGGGTCACGCGCGCGCAGTTGCTCGACGTATTGCCACCCGGCGGCGCGCGCAATGCGGTCGATGC
GGCGTTGTGGGCACTCGAATCACGACAGCAGGCCATGCCCGTCTGGCACATCGCGGGCCTCTCCGAGCCGCGCCCGTTGC
GGACCACGATGACGATCGGCGTGGGTACGCCGAACGCAATGGCCGGGGCCGCACGAAGACTGCCCCACGCCAGGGCGCTG
AAGGTCAAGCTGGACGGCAGCGCCGCCGACGCCGCACGCCTCTTCGAAATCCGCGAGGTATGCCCGGAAATACCACTCTC
TGTCGACGCGAATCAGGGCTGGTCACGCGCGCACTTCGACGCGATGCTCCCCGTGCTGCAACAGCTCGATGTCTGCATGC
TCGAACAGCCGTTTGCGATCGGTGCGGATGCCTGTCTCGAACATATCGACTGTCCGATCCCGCTCGCGGCCGACGAATCG
TTTCAGGATCTCGGCGATCTGCCCGCCGTCACGCGCCGCTACGACATCGTCAATCTCAAGCTCGACAAATGCGGTGGCCT
GACGCGCGCGTTCGATCTTGCCACCCGTGTACGCGAAGCCGGCTTGTCCGTGATGGTCGGTTGCATGGCAGGCACCTCGC
TGGCGATCGCTCCCGCGTTTCTGCTCGGGCAACAGTGCGATCACGTCGATCTCGACGGACCGATCTTCCTAGCACGCGAT
CGTGAGCCGGGCGTCGCCTACATCGACGGCCTGCTGCACTGCCCGCCCGACCTCTGGGACCACGCGGGCTTCCCTGCCTT
CTCTCGCTGA

Upstream 100 bases:

>100_bases
TTGCTGTGGTCGATCGTCTGCGTGGCGGCGTTCGGCTTCTTTGCCGCCTGCCTGCTGATGCGCGCGGCGGGCCGTGGTGT
CCGCGAGGCAATCGAACGCT

Downstream 100 bases:

>100_bases
GTTATCGACATGACATTCGACTCATCCGCCACGACGACGCAGCTCGACGCGCTGTTCGCGCGTTATCACCACGCCGATGC
CCCCGGATTGATCGCCGGCA

Product: mandelate racemase/muconate lactonizing protein

Products: 3-chloro-cis,cis-muconate

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MRNSLLPHSSMSPRTLSLSLRIERWPLAAPFHITGHVIDAARLVCVTVDDGHHAGNGEASGVFYLGETPESIVAQIDRAR
PSIEAGVTRAQLLDVLPPGGARNAVDAALWALESRQQAMPVWHIAGLSEPRPLRTTMTIGVGTPNAMAGAARRLPHARAL
KVKLDGSAADAARLFEIREVCPEIPLSVDANQGWSRAHFDAMLPVLQQLDVCMLEQPFAIGADACLEHIDCPIPLAADES
FQDLGDLPAVTRRYDIVNLKLDKCGGLTRAFDLATRVREAGLSVMVGCMAGTSLAIAPAFLLGQQCDHVDLDGPIFLARD
REPGVAYIDGLLHCPPDLWDHAGFPAFSR

Sequences:

>Translated_349_residues
MRNSLLPHSSMSPRTLSLSLRIERWPLAAPFHITGHVIDAARLVCVTVDDGHHAGNGEASGVFYLGETPESIVAQIDRAR
PSIEAGVTRAQLLDVLPPGGARNAVDAALWALESRQQAMPVWHIAGLSEPRPLRTTMTIGVGTPNAMAGAARRLPHARAL
KVKLDGSAADAARLFEIREVCPEIPLSVDANQGWSRAHFDAMLPVLQQLDVCMLEQPFAIGADACLEHIDCPIPLAADES
FQDLGDLPAVTRRYDIVNLKLDKCGGLTRAFDLATRVREAGLSVMVGCMAGTSLAIAPAFLLGQQCDHVDLDGPIFLARD
REPGVAYIDGLLHCPPDLWDHAGFPAFSR
>Mature_349_residues
MRNSLLPHSSMSPRTLSLSLRIERWPLAAPFHITGHVIDAARLVCVTVDDGHHAGNGEASGVFYLGETPESIVAQIDRAR
PSIEAGVTRAQLLDVLPPGGARNAVDAALWALESRQQAMPVWHIAGLSEPRPLRTTMTIGVGTPNAMAGAARRLPHARAL
KVKLDGSAADAARLFEIREVCPEIPLSVDANQGWSRAHFDAMLPVLQQLDVCMLEQPFAIGADACLEHIDCPIPLAADES
FQDLGDLPAVTRRYDIVNLKLDKCGGLTRAFDLATRVREAGLSVMVGCMAGTSLAIAPAFLLGQQCDHVDLDGPIFLARD
REPGVAYIDGLLHCPPDLWDHAGFPAFSR

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has a role in the recycling of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of all the L-Ala-X dipeptides, excep

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=313, Percent_Identity=42.1725239616613, Blast_Score=216, Evalue=1e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: 5.5.1.7

Molecular weight: Translated: 37540; Mature: 37540

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNSLLPHSSMSPRTLSLSLRIERWPLAAPFHITGHVIDAARLVCVTVDDGHHAGNGEAS
CCCCCCCCCCCCCEEEEEEEEEEECCCCCCCEEEHEEECCEEEEEEEECCCCCCCCCCCC
GVFYLGETPESIVAQIDRARPSIEAGVTRAQLLDVLPPGGARNAVDAALWALESRQQAMP
EEEEECCCHHHHHHHHHHCCCCHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
VWHIAGLSEPRPLRTTMTIGVGTPNAMAGAARRLPHARALKVKLDGSAADAARLFEIREV
EEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHH
CPEIPLSVDANQGWSRAHFDAMLPVLQQLDVCMLEQPFAIGADACLEHIDCPIPLAADES
CCCCCCEEECCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHCCCCCCCCCCCC
FQDLGDLPAVTRRYDIVNLKLDKCGGLTRAFDLATRVREAGLSVMVGCMAGTSLAIAPAF
HHHHCCCCHHHHEEEEEEEEECCCCCHHHHHHHHHHHHHCCHHEEEHHHCCCHHHHHHHH
LLGQQCDHVDLDGPIFLARDREPGVAYIDGLLHCPPDLWDHAGFPAFSR
HHCCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCHHHHHCCCCCCCCC
>Mature Secondary Structure
MRNSLLPHSSMSPRTLSLSLRIERWPLAAPFHITGHVIDAARLVCVTVDDGHHAGNGEAS
CCCCCCCCCCCCCEEEEEEEEEEECCCCCCCEEEHEEECCEEEEEEEECCCCCCCCCCCC
GVFYLGETPESIVAQIDRARPSIEAGVTRAQLLDVLPPGGARNAVDAALWALESRQQAMP
EEEEECCCHHHHHHHHHHCCCCHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
VWHIAGLSEPRPLRTTMTIGVGTPNAMAGAARRLPHARALKVKLDGSAADAARLFEIREV
EEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHH
CPEIPLSVDANQGWSRAHFDAMLPVLQQLDVCMLEQPFAIGADACLEHIDCPIPLAADES
CCCCCCEEECCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHCCCCCCCCCCCC
FQDLGDLPAVTRRYDIVNLKLDKCGGLTRAFDLATRVREAGLSVMVGCMAGTSLAIAPAF
HHHHCCCCHHHHEEEEEEEEECCCCCHHHHHHHHHHHHHCCHHEEEHHHCCCHHHHHHHH
LLGQQCDHVDLDGPIFLARDREPGVAYIDGLLHCPPDLWDHAGFPAFSR
HHCCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-chloro-2,5-dihydro-5-oxofuran-2-acetate

Specific reaction: 2-chloro-2,5-dihydro-5-oxofuran-2-acetate = 3-chloro-cis,cis-muconate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7499381; 9097039; 9278503 [H]