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The map label for this gene is purR [H]

Identifier: 78065815

GI number: 78065815

Start: 1296043

End: 1297068

Strand: Reverse

Name: purR [H]

Synonym: Bcep18194_A4344

Alternate gene names: 78065815

Gene position: 1297068-1296043 (Counterclockwise)

Preceding gene: 78065816

Following gene: 78065814

Centisome position: 35.11

GC content: 69.98

Gene sequence:

>1026_bases
ATGACCTCGACCATCAAGGACGTCGCTGCCCTCGCGGGCTTCTCGATCGCCACTGTCTCGCGTGCGATCAACGCCCCGCA
TACCGTCAGCCCCGCCACGCTGCAAACGATCCGCACCGCGATCGACACGCTGCAATTTCGCCCGAGCCCGCTCGGCCGGC
AATTGCGCGGCGAGCGCACCCGCCTCGTCGGCGTCGTCGTGCCGACGTTGTCGAACCCCGTGTTCGCCGACTGCCTGCAA
GGCATCGACGAACTCGCGACCGCGGCCGGCTTCAAGCTGATCCTGATGACGACCGAATACGACGCGGCGCGCGAGCGTCA
CGCGATCGAGACGCTGCGCGAGCAGCGCGTCGAAGGGCTGATCCTCACCGTCGCCGATGCGGACACGCACCCGCTGCTCG
ACATGCTCGACCACGACGGCCCGCACTACGTGCTGATGCACAACGACACGCAGCGCCGCCCGTCGGTATCGGTCGACAAC
CGCAGCGCCGCGTACGACGGCGTGCGCTTGCTGACCGCGCGTGGCCACCGCCGCGTGCTGATGCTGGCCGGCTCGCTCGC
CGCGTCGGATCGTGCGCGCCAGCGTCATCTCGGCTATGCGCAGGCCCTCGAGGAAAGCGGCGTCGCGACGCTGCCGCCGG
TCGAAGTCGACTTCAACGCGCCCGAGTTGCCCGACGCGGTGCTCGCGCATCTCACCGCGCACGCGACGCGCCCGACCGCC
CTCTTCTGCAGCAACGACCTGCTCGCGATGGTCGTGATGCGCGGCCTGCGCCGCGCGGGTTTCTCGATCCCGGACGACCT
GTCGGTGCTCGGCTTCGACGGAATCGCGATCGGCGAACTGCTCGCGCCGCCGCTCGCGAGCGTCGCGACGCCGAACCGCG
ACATCGGCCGCCACGCATGGCAGCGTCTCGTCGAATGCATCGGCGGCGCAGCGATCGAACGCACGTCGCTGATCCTGCCG
CACGTGGTGCGCGACGGTGCGACGGTCGCCCCGCCGGCCACCGACCTGCAATTGCGCAAGGCTTGA

Upstream 100 bases:

>100_bases
GTGTCGTGCTTTTTGCGAAACAAGCGTCGCTTGCGTCATCGCGAAGTCACAAACGTTTTCGATAATTTTCTGGCCGAAAA
CGTTTACATCGCAAGATTTC

Downstream 100 bases:

>100_bases
ACGCGCGCCGCGCCGCCACACACGTACACAAGCACACAACCACACCACCCCAGCGCCTCACACTCGCCCGGAGACCCACC
GTGTCCTTTCGCCTGACCTC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MTSTIKDVAALAGFSIATVSRAINAPHTVSPATLQTIRTAIDTLQFRPSPLGRQLRGERTRLVGVVVPTLSNPVFADCLQ
GIDELATAAGFKLILMTTEYDAARERHAIETLREQRVEGLILTVADADTHPLLDMLDHDGPHYVLMHNDTQRRPSVSVDN
RSAAYDGVRLLTARGHRRVLMLAGSLAASDRARQRHLGYAQALEESGVATLPPVEVDFNAPELPDAVLAHLTAHATRPTA
LFCSNDLLAMVVMRGLRRAGFSIPDDLSVLGFDGIAIGELLAPPLASVATPNRDIGRHAWQRLVECIGGAAIERTSLILP
HVVRDGATVAPPATDLQLRKA

Sequences:

>Translated_341_residues
MTSTIKDVAALAGFSIATVSRAINAPHTVSPATLQTIRTAIDTLQFRPSPLGRQLRGERTRLVGVVVPTLSNPVFADCLQ
GIDELATAAGFKLILMTTEYDAARERHAIETLREQRVEGLILTVADADTHPLLDMLDHDGPHYVLMHNDTQRRPSVSVDN
RSAAYDGVRLLTARGHRRVLMLAGSLAASDRARQRHLGYAQALEESGVATLPPVEVDFNAPELPDAVLAHLTAHATRPTA
LFCSNDLLAMVVMRGLRRAGFSIPDDLSVLGFDGIAIGELLAPPLASVATPNRDIGRHAWQRLVECIGGAAIERTSLILP
HVVRDGATVAPPATDLQLRKA
>Mature_340_residues
TSTIKDVAALAGFSIATVSRAINAPHTVSPATLQTIRTAIDTLQFRPSPLGRQLRGERTRLVGVVVPTLSNPVFADCLQG
IDELATAAGFKLILMTTEYDAARERHAIETLREQRVEGLILTVADADTHPLLDMLDHDGPHYVLMHNDTQRRPSVSVDNR
SAAYDGVRLLTARGHRRVLMLAGSLAASDRARQRHLGYAQALEESGVATLPPVEVDFNAPELPDAVLAHLTAHATRPTAL
FCSNDLLAMVVMRGLRRAGFSIPDDLSVLGFDGIAIGELLAPPLASVATPNRDIGRHAWQRLVECIGGAAIERTSLILPH
VVRDGATVAPPATDLQLRKA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=342, Percent_Identity=29.2397660818713, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1790194, Length=336, Percent_Identity=30.952380952381, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1789202, Length=297, Percent_Identity=31.6498316498317, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1790715, Length=345, Percent_Identity=26.6666666666667, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI48994940, Length=335, Percent_Identity=27.7611940298507, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1788474, Length=327, Percent_Identity=30.5810397553517, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787948, Length=312, Percent_Identity=28.2051282051282, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1789068, Length=338, Percent_Identity=29.585798816568, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1786540, Length=330, Percent_Identity=27.2727272727273, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787580, Length=321, Percent_Identity=26.1682242990654, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1787906, Length=334, Percent_Identity=25.748502994012, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790689, Length=320, Percent_Identity=23.4375, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786268, Length=325, Percent_Identity=24.3076923076923, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36696; Mature: 36564

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTIKDVAALAGFSIATVSRAINAPHTVSPATLQTIRTAIDTLQFRPSPLGRQLRGERT
CCCHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCE
RLVGVVVPTLSNPVFADCLQGIDELATAAGFKLILMTTEYDAARERHAIETLREQRVEGL
EEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHCEE
ILTVADADTHPLLDMLDHDGPHYVLMHNDTQRRPSVSVDNRSAAYDGVRLLTARGHRRVL
EEEEECCCCCHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHCCEEEEECCCCCEEE
MLAGSLAASDRARQRHLGYAQALEESGVATLPPVEVDFNAPELPDAVLAHLTAHATRPTA
EEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCE
LFCSNDLLAMVVMRGLRRAGFSIPDDLSVLGFDGIAIGELLAPPLASVATPNRDIGRHAW
EEECCHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHCCCHHHHCCCCCHHHHHHH
QRLVECIGGAAIERTSLILPHVVRDGATVAPPATDLQLRKA
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECC
>Mature Secondary Structure 
TSTIKDVAALAGFSIATVSRAINAPHTVSPATLQTIRTAIDTLQFRPSPLGRQLRGERT
CCHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCE
RLVGVVVPTLSNPVFADCLQGIDELATAAGFKLILMTTEYDAARERHAIETLREQRVEGL
EEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHCEE
ILTVADADTHPLLDMLDHDGPHYVLMHNDTQRRPSVSVDNRSAAYDGVRLLTARGHRRVL
EEEEECCCCCHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHCCEEEEECCCCCEEE
MLAGSLAASDRARQRHLGYAQALEESGVATLPPVEVDFNAPELPDAVLAHLTAHATRPTA
EEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCE
LFCSNDLLAMVVMRGLRRAGFSIPDDLSVLGFDGIAIGELLAPPLASVATPNRDIGRHAW
EEECCHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHCCCHHHHCCCCCHHHHHHH
QRLVECIGGAAIERTSLILPHVVRDGATVAPPATDLQLRKA
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA