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The map label for this gene is potD [C]

Identifier: 78065814

GI number: 78065814

Start: 1294934

End: 1296046

Strand: Reverse

Name: potD [C]

Synonym: Bcep18194_A4343

Alternate gene names: 78065814

Gene position: 1296046-1294934 (Counterclockwise)

Preceding gene: 78065815

Following gene: 78065813

Centisome position: 35.08

GC content: 65.14

Gene sequence:

>1113_bases
TTGAACGCGCGCCGCGCCGCCACACACGTACACAAGCACACAACCACACCACCCCAGCGCCTCACACTCGCCCGGAGACC
CACCGTGTCCTTTCGCCTGACCTCGCTGCTGCGCGTGCTTGCAGCTCCCGTAGCCTGCGCCGCGCTCATCGCCGGCGCGC
CCGCCGCCCACGCCGACGAAACGGCGATCTGCTACAACTGCCCGCCCGAATGGGCCGACTGGGCCGCGCAGATTGCGGCG
ATCAAGCAGAAGACCGGCATCCGCGTGCCGTTCGACAACAAGAACTCGGGCCAGTCGATCGCGCAGTTGATCGCCGAGCA
GAAGAGCCCGGTCGCCGACGTCGTCTACCTCGGCGTGTCGTCGGCGTTCCAGGCGAAGGACAAGGGTGTGATCGCGCCGT
ACAAGCCCGCGCACTGGAACGACATCCCCGCGAACCTGAAGGACCCGCAGGGCTACTGGTTTGCGATCCACTCGGGCACG
CTCGGCTTCTTCGTGAACAAGGACGCGCTCGACGGCAAGCCGGTGCCGCGCTCGTGGGCCGATCTGCTGAAGCCGGAATA
CAAGGGCATGGTCGGCTATCTCGATCCATCGAGTGCGTTCGTCGGCTATGCGGGCGCGGTCGCCGTGAACCAGGCACTTG
GCGGCAGTCTCGACAACTTCAAGCCGGCGCTCGACTGGTTCAGGAAGCTGAAGGCGAACGCGCCGATCGTGCCGAAGCAG
ACCGCGTATGCGCGCGTGCTGTCGGGCGAGATTCCGATCCTGCTCGACTACGACTTCGACGCGTATCGCGCGAAGTACAA
GGACAGCGCGAACGTCGAGTTCGTGATTCCGAAGGAAGGCACGATCGCGGTGCCGTACGTGATGAGTCTCGTGAAGGGCG
CGCCGCACGACGCGAACGGCAAGAAGGTGCTCGACTTCGTGCTGTCGGACGAAGGCCAGAAGTTGTGGGCCAATGCGTAC
CTGCGACCGGTGCGCGCGCAGACGCTCAGCGCCGACATCGCATCGAAGTTCCTGCCGGCGAGCGAATATGCCCGCGCGAA
ATCGGTCGACTTCGGCAAGATGGCGGCCGGCCAGCAGGCATTCGGCCAGCAATACCTGCAGGTGATGCAGTAA

Upstream 100 bases:

>100_bases
GCGGCGCAGCGATCGAACGCACGTCGCTGATCCTGCCGCACGTGGTGCGCGACGGTGCGACGGTCGCCCCGCCGGCCACC
GACCTGCAATTGCGCAAGGC

Downstream 100 bases:

>100_bases
GCAGCCGGACGCCATCGATGCTCGACCTGACTTTCCCGCTGCGCTGGCGCGTCGCGCTCGTCGCGCCGGCGCTTGCGGTG
TTCGCCGCGTTCTGGCTGCT

Product: ABC transporter, periplasmic ligand binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MNARRAATHVHKHTTTPPQRLTLARRPTVSFRLTSLLRVLAAPVACAALIAGAPAAHADETAICYNCPPEWADWAAQIAA
IKQKTGIRVPFDNKNSGQSIAQLIAEQKSPVADVVYLGVSSAFQAKDKGVIAPYKPAHWNDIPANLKDPQGYWFAIHSGT
LGFFVNKDALDGKPVPRSWADLLKPEYKGMVGYLDPSSAFVGYAGAVAVNQALGGSLDNFKPALDWFRKLKANAPIVPKQ
TAYARVLSGEIPILLDYDFDAYRAKYKDSANVEFVIPKEGTIAVPYVMSLVKGAPHDANGKKVLDFVLSDEGQKLWANAY
LRPVRAQTLSADIASKFLPASEYARAKSVDFGKMAAGQQAFGQQYLQVMQ

Sequences:

>Translated_370_residues
MNARRAATHVHKHTTTPPQRLTLARRPTVSFRLTSLLRVLAAPVACAALIAGAPAAHADETAICYNCPPEWADWAAQIAA
IKQKTGIRVPFDNKNSGQSIAQLIAEQKSPVADVVYLGVSSAFQAKDKGVIAPYKPAHWNDIPANLKDPQGYWFAIHSGT
LGFFVNKDALDGKPVPRSWADLLKPEYKGMVGYLDPSSAFVGYAGAVAVNQALGGSLDNFKPALDWFRKLKANAPIVPKQ
TAYARVLSGEIPILLDYDFDAYRAKYKDSANVEFVIPKEGTIAVPYVMSLVKGAPHDANGKKVLDFVLSDEGQKLWANAY
LRPVRAQTLSADIASKFLPASEYARAKSVDFGKMAAGQQAFGQQYLQVMQ
>Mature_370_residues
MNARRAATHVHKHTTTPPQRLTLARRPTVSFRLTSLLRVLAAPVACAALIAGAPAAHADETAICYNCPPEWADWAAQIAA
IKQKTGIRVPFDNKNSGQSIAQLIAEQKSPVADVVYLGVSSAFQAKDKGVIAPYKPAHWNDIPANLKDPQGYWFAIHSGT
LGFFVNKDALDGKPVPRSWADLLKPEYKGMVGYLDPSSAFVGYAGAVAVNQALGGSLDNFKPALDWFRKLKANAPIVPKQ
TAYARVLSGEIPILLDYDFDAYRAKYKDSANVEFVIPKEGTIAVPYVMSLVKGAPHDANGKKVLDFVLSDEGQKLWANAY
LRPVRAQTLSADIASKFLPASEYARAKSVDFGKMAAGQQAFGQQYLQVMQ

Specific function: Required For The Activity Of The Bacterial Periplasmic Transport System Of Putrescine And Spermidine. Polyamine Binding Protein. [C]

COG id: COG1840

COG function: function code P; ABC-type Fe3+ transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40186; Mature: 40186

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNARRAATHVHKHTTTPPQRLTLARRPTVSFRLTSLLRVLAAPVACAALIAGAPAAHADE
CCCHHHHHHHHHCCCCCHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TAICYNCPPEWADWAAQIAAIKQKTGIRVPFDNKNSGQSIAQLIAEQKSPVADVVYLGVS
CEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHEEEECCC
SAFQAKDKGVIAPYKPAHWNDIPANLKDPQGYWFAIHSGTLGFFVNKDALDGKPVPRSWA
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEEEEEECCCCCCCCCCHHHH
DLLKPEYKGMVGYLDPSSAFVGYAGAVAVNQALGGSLDNFKPALDWFRKLKANAPIVPKQ
HHHCHHHCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCH
TAYARVLSGEIPILLDYDFDAYRAKYKDSANVEFVIPKEGTIAVPYVMSLVKGAPHDANG
HHHHHHHCCCCCEEEECCCHHHHHHCCCCCCEEEEECCCCCEEHHHHHHHHHCCCCCCCC
KKVLDFVLSDEGQKLWANAYLRPVRAQTLSADIASKFLPASEYARAKSVDFGKMAAGQQA
CEEEHEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHCCHHH
FGQQYLQVMQ
HHHHHHHHHC
>Mature Secondary Structure
MNARRAATHVHKHTTTPPQRLTLARRPTVSFRLTSLLRVLAAPVACAALIAGAPAAHADE
CCCHHHHHHHHHCCCCCHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TAICYNCPPEWADWAAQIAAIKQKTGIRVPFDNKNSGQSIAQLIAEQKSPVADVVYLGVS
CEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHEEEECCC
SAFQAKDKGVIAPYKPAHWNDIPANLKDPQGYWFAIHSGTLGFFVNKDALDGKPVPRSWA
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEEEEEECCCCCCCCCCHHHH
DLLKPEYKGMVGYLDPSSAFVGYAGAVAVNQALGGSLDNFKPALDWFRKLKANAPIVPKQ
HHHCHHHCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCH
TAYARVLSGEIPILLDYDFDAYRAKYKDSANVEFVIPKEGTIAVPYVMSLVKGAPHDANG
HHHHHHHCCCCCEEEECCCHHHHHHCCCCCCEEEEECCCCCEEHHHHHHHHHCCCCCCCC
KKVLDFVLSDEGQKLWANAYLRPVRAQTLSADIASKFLPASEYARAKSVDFGKMAAGQQA
CEEEHEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHCCHHH
FGQQYLQVMQ
HHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]