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The map label for this gene is gudD [H]

Identifier: 78065747

GI number: 78065747

Start: 1209261

End: 1210613

Strand: Reverse

Name: gudD [H]

Synonym: Bcep18194_A4275

Alternate gene names: 78065747

Gene position: 1210613-1209261 (Counterclockwise)

Preceding gene: 78065749

Following gene: 78065743

Centisome position: 32.77

GC content: 69.55

Gene sequence:

>1353_bases
ATGAACGCCGTCACTGCCTCGCCCTCCCACGACACGCCGCGTATCGTCGACCTGCAAGCGATTCCGGTCGCCGGCCACGA
CAGCATGCTGCTCAACCTGAGCGGCGCACACGGCCCGTTCTTCACCCGCAACCTCGTGATCCTGAAGGACAGCGCGGGCC
GGACCGGCGTCGGTGAAGTACCGGGCGGCGAAAGCATCCGCCGCACGCTCGACGATGCGCGCGCGCTCGTCGTCGGCCAG
CCGGTCGGCAATTACCACGCGGTGCTCAACGACGTGCGGCGCACCTTCGCCGACCGCGACGCGAGCGGCCGCGGGCTGCA
GACCTTCGACCTGCGCACGACGATCCACGCGGTCACCGCACTCGAAGCCGCGCTGCTCGACCTGCTCGGCCAGCATCTCG
GCGTGCCGGTCGCCGCGTTGCTCGGCGAGGGCCAGCAGCGCGAGCGCGTCGAAATGCTCGGCTACCTGTTCTACATCGGC
GACCGCACCAGGACCGCGCTCGCCTACCGCGACGGCAGCGGCGCGACCGACGACTGGACGCGCGTGCGCGACGAAGCCGC
ACTGACACCCGAGGCCGTCGTACGGCTCGCCGAGGCCGCGCATGCGCGCTACGGCTTCAACGACTTCAAGCTGAAGGGCG
GCGTGTTCGAAGGCGCGAGCGAGATCGAGGCCGTGACGGCACTCGCCGAGCGCTTCCCCGACGCGCGCGTGACGCTCGAC
CCGAACGGCGCGTGGTCGCTCGACGAGGCCGTGCGGCTGTGCCGTGATCAGCATCACGTGCTCGCCTACGCGGAAGATCC
GTGCGGCGCGGAGAACGGCTACTCGGGCCGCGAGGTGATGGCCGAATTCCGCCGCGCGACGGGGCTGCCGACGGCCACCA
ACATGATTGCGACAGACTGGCGGCAAATGGGTCACGCGGTGCAGCTGCAGGCCGTCGACATCCCGCTCGCCGATCCGCAC
TTCTGGACGATGCAGGGTTCGGTGCGCGTCGCGCAGATGTGCCGCGACTGGGGCCTCACGTGGGGCTCGCACTCGAACAA
CCACTTCGACGTGTCGCTCGCGATGTTCACGCACGTCGCGGCCGCCGCGCCGGGCCAGGTCACCGCGATCGACACGCACT
GGATCTGGCAGGACGGCGAGCGGCTGACGCGCGAACCGCTGAAGATCGAGAACGGGCTGGTAGAGGTGCCGAAGCGGCCG
GGGCTCGGCATCGATATCGACATGGATGCCGTCGCGGTCGCGCACGAACTGTACAAGCAGCACGGGCTCGGTGCCCGCGA
CGACGCGGCGGCGATGCAGTACCTGATTCCGGGTTGGACCTTCAACAACAAGCGCCCTTGCCTCGTGCGCTGA

Upstream 100 bases:

>100_bases
TGCGCGCCGCGCACGTCGGTTGTCGAATGATCGCACCTCCCGCCGCGCGCGGCAAAGCTTTTGCCGCACGGCATTGCCCA
CCCTTTCGCCATCGCCGCCC

Downstream 100 bases:

>100_bases
GCACGGGACGGACCGGGCGGCAGGATCGCCCGGTCGAGCCGGAATCGAAAGAAGCGATGCGGCCTGCGGCCGGGCTGGTG
GGTATCGGGCGGAAACGCTG

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: GDH; GlucD [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MNAVTASPSHDTPRIVDLQAIPVAGHDSMLLNLSGAHGPFFTRNLVILKDSAGRTGVGEVPGGESIRRTLDDARALVVGQ
PVGNYHAVLNDVRRTFADRDASGRGLQTFDLRTTIHAVTALEAALLDLLGQHLGVPVAALLGEGQQRERVEMLGYLFYIG
DRTRTALAYRDGSGATDDWTRVRDEAALTPEAVVRLAEAAHARYGFNDFKLKGGVFEGASEIEAVTALAERFPDARVTLD
PNGAWSLDEAVRLCRDQHHVLAYAEDPCGAENGYSGREVMAEFRRATGLPTATNMIATDWRQMGHAVQLQAVDIPLADPH
FWTMQGSVRVAQMCRDWGLTWGSHSNNHFDVSLAMFTHVAAAAPGQVTAIDTHWIWQDGERLTREPLKIENGLVEVPKRP
GLGIDIDMDAVAVAHELYKQHGLGARDDAAAMQYLIPGWTFNNKRPCLVR

Sequences:

>Translated_450_residues
MNAVTASPSHDTPRIVDLQAIPVAGHDSMLLNLSGAHGPFFTRNLVILKDSAGRTGVGEVPGGESIRRTLDDARALVVGQ
PVGNYHAVLNDVRRTFADRDASGRGLQTFDLRTTIHAVTALEAALLDLLGQHLGVPVAALLGEGQQRERVEMLGYLFYIG
DRTRTALAYRDGSGATDDWTRVRDEAALTPEAVVRLAEAAHARYGFNDFKLKGGVFEGASEIEAVTALAERFPDARVTLD
PNGAWSLDEAVRLCRDQHHVLAYAEDPCGAENGYSGREVMAEFRRATGLPTATNMIATDWRQMGHAVQLQAVDIPLADPH
FWTMQGSVRVAQMCRDWGLTWGSHSNNHFDVSLAMFTHVAAAAPGQVTAIDTHWIWQDGERLTREPLKIENGLVEVPKRP
GLGIDIDMDAVAVAHELYKQHGLGARDDAAAMQYLIPGWTFNNKRPCLVR
>Mature_450_residues
MNAVTASPSHDTPRIVDLQAIPVAGHDSMLLNLSGAHGPFFTRNLVILKDSAGRTGVGEVPGGESIRRTLDDARALVVGQ
PVGNYHAVLNDVRRTFADRDASGRGLQTFDLRTTIHAVTALEAALLDLLGQHLGVPVAALLGEGQQRERVEMLGYLFYIG
DRTRTALAYRDGSGATDDWTRVRDEAALTPEAVVRLAEAAHARYGFNDFKLKGGVFEGASEIEAVTALAERFPDARVTLD
PNGAWSLDEAVRLCRDQHHVLAYAEDPCGAENGYSGREVMAEFRRATGLPTATNMIATDWRQMGHAVQLQAVDIPLADPH
FWTMQGSVRVAQMCRDWGLTWGSHSNNHFDVSLAMFTHVAAAAPGQVTAIDTHWIWQDGERLTREPLKIENGLVEVPKRP
GLGIDIDMDAVAVAHELYKQHGLGARDDAAAMQYLIPGWTFNNKRPCLVR

Specific function: Catalyzes the dehydration of glucarate to 5-keto-4- deoxy-D-glucarate (5-kdGluc) [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789150, Length=445, Percent_Identity=72.5842696629214, Blast_Score=696, Evalue=0.0,
Organism=Escherichia coli, GI1789151, Length=445, Percent_Identity=63.3707865168539, Blast_Score=558, Evalue=1e-160,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017653
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.40 [H]

Molecular weight: Translated: 49134; Mature: 49134

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAVTASPSHDTPRIVDLQAIPVAGHDSMLLNLSGAHGPFFTRNLVILKDSAGRTGVGEV
CCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEECCCCCCCEECEEEEEECCCCCCCCCCC
PGGESIRRTLDDARALVVGQPVGNYHAVLNDVRRTFADRDASGRGLQTFDLRTTIHAVTA
CCCHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHH
LEAALLDLLGQHLGVPVAALLGEGQQRERVEMLGYLFYIGDRTRTALAYRDGSGATDDWT
HHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHEEEEEECCCCEEEEEEECCCCCCCHHH
RVRDEAALTPEAVVRLAEAAHARYGFNDFKLKGGVFEGASEIEAVTALAERFPDARVTLD
HHHHHHCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEC
PNGAWSLDEAVRLCRDQHHVLAYAEDPCGAENGYSGREVMAEFRRATGLPTATNMIATDW
CCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
RQMGHAVQLQAVDIPLADPHFWTMQGSVRVAQMCRDWGLTWGSHSNNHFDVSLAMFTHVA
HHCCCEEEEEEEECCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHH
AAAPGQVTAIDTHWIWQDGERLTREPLKIENGLVEVPKRPGLGIDIDMDAVAVAHELYKQ
HCCCCCEEEEEEEEEECCCHHHHCCCHHHCCCEEECCCCCCCCEEECHHHHHHHHHHHHH
HGLGARDDAAAMQYLIPGWTFNNKRPCLVR
HCCCCCCHHHHHHHHCCCEECCCCCCCCCC
>Mature Secondary Structure
MNAVTASPSHDTPRIVDLQAIPVAGHDSMLLNLSGAHGPFFTRNLVILKDSAGRTGVGEV
CCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEECCCCCCCEECEEEEEECCCCCCCCCCC
PGGESIRRTLDDARALVVGQPVGNYHAVLNDVRRTFADRDASGRGLQTFDLRTTIHAVTA
CCCHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHH
LEAALLDLLGQHLGVPVAALLGEGQQRERVEMLGYLFYIGDRTRTALAYRDGSGATDDWT
HHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHEEEEEECCCCEEEEEEECCCCCCCHHH
RVRDEAALTPEAVVRLAEAAHARYGFNDFKLKGGVFEGASEIEAVTALAERFPDARVTLD
HHHHHHCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEC
PNGAWSLDEAVRLCRDQHHVLAYAEDPCGAENGYSGREVMAEFRRATGLPTATNMIATDW
CCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
RQMGHAVQLQAVDIPLADPHFWTMQGSVRVAQMCRDWGLTWGSHSNNHFDVSLAMFTHVA
HHCCCEEEEEEEECCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHH
AAAPGQVTAIDTHWIWQDGERLTREPLKIENGLVEVPKRPGLGIDIDMDAVAVAHELYKQ
HCCCCCEEEEEEEEEECCCHHHHCCCHHHCCCEEECCCCCCCCEEECHHHHHHHHHHHHH
HGLGARDDAAAMQYLIPGWTFNNKRPCLVR
HCCCCCCHHHHHHHHCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9772161 [H]