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The map label for this gene is livK [C]

Identifier: 78065749

GI number: 78065749

Start: 1211871

End: 1213418

Strand: Reverse

Name: livK [C]

Synonym: Bcep18194_A4277

Alternate gene names: 78065749

Gene position: 1213418-1211871 (Counterclockwise)

Preceding gene: 78065750

Following gene: 78065747

Centisome position: 32.85

GC content: 67.05

Gene sequence:

>1548_bases
ATGCCGAAGCCGCATCGGACGCCCTGTCCGACTGACGGCGTCCCGTGCGCGGCGCGGCCCCGGCGGGCCGCGCCGCCTCC
CTGCACCTCCTCCCTCTCCCCCCGTTCCGTTCGCGACGGGAAACTCCCGATACCGGCTGCCGCCGCACGCGCGGAAGCTA
TGCGCAAATCCGCACCGAATGCGCGGCGAATTGCCAAGACCCGGGCATATCGGCGACCTATATATCGAGGCGATCGGGGC
TCTTCTTCGCGGAGCCTGACGGGAGATTCTTCCGAGGATAACGATGCCGACCTCCCCGCCCGATTCAGAGAAGCGGTCGA
ACCGCCCGCCCGTCGCACGCGTTCGGCCCACCCGAGCGCATGCGGTACTTACTCGCAGGTGTCGAACAAGAGGAACAACA
TGAAATTCCGTCATTCCCTGCTGTCGGTGTCGATTGCATCCGCGCTTGCCCTCCTCTCGCAACAGGCCGCCCAGGCCGCC
GATTCGACCGACGTGAAGGTCGGCTTCGCCGCGCCGCTCACGGGCGTGAACGCCGGTTACGGCAAGGACCTGCAGAACGG
CGTGCAGCTCGCGCTCGACGATGCCGCCGCGCAGAAGGTGCAGATCGCCGGCAAGCCCGCGCACTTCAACCTCATCGTGC
AGGACGACCAGGCCGACCCGCGCATCGGCGTGCAGGCCGCGCAGGCGCTCGTCGACCAGAACGTGTCGGTCGTGGTCGGC
CACTTCAACTCGGGGACGACGATTCCGGCTTCCGTCGTCTACGACAAGGCCGGCATCCCGGTGATCGATCCGGCCGCGAC
CAACCCGACGCTCACCGCGCGCGGGCTCGCGAACATGTTCATGGTGATCGCGACCGACGGCCAGAACGCCGGCAACGCGG
GCAAGTACGCGGTCGACGTGACGAAGGCGAAGCGCATCGCGATCATCGACGACCGCACCGCGTTCGGCCAGGGCGAGGCC
GACGAGTTCGAGAAGGCCGTGAAGGCCGCGGGCGGGAACCTCGTCGGCCGCGAATTCACCAGCAACCAGGCGGTCGACTT
CCGCGCGCAGATCACGAGCCTGAAGGCGAAGAACCCCGACCTGATCTTCTTCGGCGGCCTCGACTCGCTCGCCGCGAACT
TCATCAAGCAGATGCGCCAGCTCGGGCTGAACGCGCAATTCGTCGGCGGCGGCGGCGTGAAGGACAACGAGTTCATCAAG
ATCGCGGGCCCGGCCGCCGAAGGCGCGATGGCGTGGGAATACGGCCGGCCGCTCGACGAGCTGCCGCAAGGGAAGGATTT
CGAGCAGCGCTTCAAGAAGCGCTTCGGCGTCGATGTGCTGTCGTACGCGCAATTCGGCTACGACGCGGCGTGGGCGGCGA
TCAAGGCGATGCAGGCGGCCGGCTCGACCGACCCGAAGGTCTATCGCCCGGCGCTGAAGAAGATCGACTTCGAAGGGGTC
ACCGGCCGCATCTCGTTCGCGAACGACGGCTCGCTGAAGAGCGGGATGTCGACGCTGTACCAGGTGAAGAGCGGCGCGTG
GAAGACGATCACGACGAAGGGAGGCTGA

Upstream 100 bases:

>100_bases
GGCCGGCTGCTCGGCTTCCTGCGCCGCTTCGCGGGCCACGTCGAGACGGGCCGCCCGAGCATCAGCGATCCGCTGATCCG
CACGCACGCATCGCACGACG

Downstream 100 bases:

>100_bases
TCGGCGGTGCCGGCGGCCGGCGGCGGAAAAGCCGCCCGGCCCCGGTGATCGATGAAGGGATATCGATCTCGTATGACGAC
TGATGTTGAAGCGATTCGAC

Product: branched chain amino acid ABC transporter periplasmic ligand-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MPKPHRTPCPTDGVPCAARPRRAAPPPCTSSLSPRSVRDGKLPIPAAAARAEAMRKSAPNARRIAKTRAYRRPIYRGDRG
SSSRSLTGDSSEDNDADLPARFREAVEPPARRTRSAHPSACGTYSQVSNKRNNMKFRHSLLSVSIASALALLSQQAAQAA
DSTDVKVGFAAPLTGVNAGYGKDLQNGVQLALDDAAAQKVQIAGKPAHFNLIVQDDQADPRIGVQAAQALVDQNVSVVVG
HFNSGTTIPASVVYDKAGIPVIDPAATNPTLTARGLANMFMVIATDGQNAGNAGKYAVDVTKAKRIAIIDDRTAFGQGEA
DEFEKAVKAAGGNLVGREFTSNQAVDFRAQITSLKAKNPDLIFFGGLDSLAANFIKQMRQLGLNAQFVGGGGVKDNEFIK
IAGPAAEGAMAWEYGRPLDELPQGKDFEQRFKKRFGVDVLSYAQFGYDAAWAAIKAMQAAGSTDPKVYRPALKKIDFEGV
TGRISFANDGSLKSGMSTLYQVKSGAWKTITTKGG

Sequences:

>Translated_515_residues
MPKPHRTPCPTDGVPCAARPRRAAPPPCTSSLSPRSVRDGKLPIPAAAARAEAMRKSAPNARRIAKTRAYRRPIYRGDRG
SSSRSLTGDSSEDNDADLPARFREAVEPPARRTRSAHPSACGTYSQVSNKRNNMKFRHSLLSVSIASALALLSQQAAQAA
DSTDVKVGFAAPLTGVNAGYGKDLQNGVQLALDDAAAQKVQIAGKPAHFNLIVQDDQADPRIGVQAAQALVDQNVSVVVG
HFNSGTTIPASVVYDKAGIPVIDPAATNPTLTARGLANMFMVIATDGQNAGNAGKYAVDVTKAKRIAIIDDRTAFGQGEA
DEFEKAVKAAGGNLVGREFTSNQAVDFRAQITSLKAKNPDLIFFGGLDSLAANFIKQMRQLGLNAQFVGGGGVKDNEFIK
IAGPAAEGAMAWEYGRPLDELPQGKDFEQRFKKRFGVDVLSYAQFGYDAAWAAIKAMQAAGSTDPKVYRPALKKIDFEGV
TGRISFANDGSLKSGMSTLYQVKSGAWKTITTKGG
>Mature_514_residues
PKPHRTPCPTDGVPCAARPRRAAPPPCTSSLSPRSVRDGKLPIPAAAARAEAMRKSAPNARRIAKTRAYRRPIYRGDRGS
SSRSLTGDSSEDNDADLPARFREAVEPPARRTRSAHPSACGTYSQVSNKRNNMKFRHSLLSVSIASALALLSQQAAQAAD
STDVKVGFAAPLTGVNAGYGKDLQNGVQLALDDAAAQKVQIAGKPAHFNLIVQDDQADPRIGVQAAQALVDQNVSVVVGH
FNSGTTIPASVVYDKAGIPVIDPAATNPTLTARGLANMFMVIATDGQNAGNAGKYAVDVTKAKRIAIIDDRTAFGQGEAD
EFEKAVKAAGGNLVGREFTSNQAVDFRAQITSLKAKNPDLIFFGGLDSLAANFIKQMRQLGLNAQFVGGGGVKDNEFIKI
AGPAAEGAMAWEYGRPLDELPQGKDFEQRFKKRFGVDVLSYAQFGYDAAWAAIKAMQAAGSTDPKVYRPALKKIDFEGVT
GRISFANDGSLKSGMSTLYQVKSGAWKTITTKGG

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=358, Percent_Identity=31.5642458100559, Blast_Score=145, Evalue=7e-36,
Organism=Escherichia coli, GI48994941, Length=332, Percent_Identity=30.421686746988, Blast_Score=135, Evalue=6e-33,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 54637; Mature: 54506

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKPHRTPCPTDGVPCAARPRRAAPPPCTSSLSPRSVRDGKLPIPAAAARAEAMRKSAPN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
ARRIAKTRAYRRPIYRGDRGSSSRSLTGDSSEDNDADLPARFREAVEPPARRTRSAHPSA
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHCCCCCCC
CGTYSQVSNKRNNMKFRHSLLSVSIASALALLSQQAAQAADSTDVKVGFAAPLTGVNAGY
CCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
GKDLQNGVQLALDDAAAQKVQIAGKPAHFNLIVQDDQADPRIGVQAAQALVDQNVSVVVG
CCCHHHCCEEEECHHHHHEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEE
HFNSGTTIPASVVYDKAGIPVIDPAATNPTLTARGLANMFMVIATDGQNAGNAGKYAVDV
ECCCCCCCCHHEEECCCCCCEECCCCCCCCEEHHHHCCEEEEEEECCCCCCCCCCEEEEE
TKAKRIAIIDDRTAFGQGEADEFEKAVKAAGGNLVGREFTSNQAVDFRAQITSLKAKNPD
ECCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCEECCCCCCCCCEEHHHHHHHHCCCCCC
LIFFGGLDSLAANFIKQMRQLGLNAQFVGGGGVKDNEFIKIAGPAAEGAMAWEYGRPLDE
EEEECCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEECCCCCCCEEECCCCCHHH
LPQGKDFEQRFKKRFGVDVLSYAQFGYDAAWAAIKAMQAAGSTDPKVYRPALKKIDFEGV
CCCCCCHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCC
TGRISFANDGSLKSGMSTLYQVKSGAWKTITTKGG
EEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCC
>Mature Secondary Structure 
PKPHRTPCPTDGVPCAARPRRAAPPPCTSSLSPRSVRDGKLPIPAAAARAEAMRKSAPN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
ARRIAKTRAYRRPIYRGDRGSSSRSLTGDSSEDNDADLPARFREAVEPPARRTRSAHPSA
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHCCCCCCC
CGTYSQVSNKRNNMKFRHSLLSVSIASALALLSQQAAQAADSTDVKVGFAAPLTGVNAGY
CCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
GKDLQNGVQLALDDAAAQKVQIAGKPAHFNLIVQDDQADPRIGVQAAQALVDQNVSVVVG
CCCHHHCCEEEECHHHHHEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEE
HFNSGTTIPASVVYDKAGIPVIDPAATNPTLTARGLANMFMVIATDGQNAGNAGKYAVDV
ECCCCCCCCHHEEECCCCCCEECCCCCCCCEEHHHHCCEEEEEEECCCCCCCCCCEEEEE
TKAKRIAIIDDRTAFGQGEADEFEKAVKAAGGNLVGREFTSNQAVDFRAQITSLKAKNPD
ECCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCEECCCCCCCCCEEHHHHHHHHCCCCCC
LIFFGGLDSLAANFIKQMRQLGLNAQFVGGGGVKDNEFIKIAGPAAEGAMAWEYGRPLDE
EEEECCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEECCCCCCCEEECCCCCHHH
LPQGKDFEQRFKKRFGVDVLSYAQFGYDAAWAAIKAMQAAGSTDPKVYRPALKKIDFEGV
CCCCCCHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCC
TGRISFANDGSLKSGMSTLYQVKSGAWKTITTKGG
EEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA