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The map label for this gene is pmbA [H]

Identifier: 78065667

GI number: 78065667

Start: 1128532

End: 1129902

Strand: Direct

Name: pmbA [H]

Synonym: Bcep18194_A4195

Alternate gene names: 78065667

Gene position: 1128532-1129902 (Clockwise)

Preceding gene: 78065664

Following gene: 78065670

Centisome position: 30.55

GC content: 67.32

Gene sequence:

>1371_bases
ATGGCAGCCAATCTCGACGTACAAGCGCGCTATTTCCCGCACACGCAGGACCAGCTCAAAGAAATCGCCTCGGACATCCT
TCGCCATGCGAAGGCACTCGGCGCGACGGACGCCGCGACCGAAATCTCCGAGGGCGACGGCCTGTCGGTGTCGGTGCGCC
GCGGCGAGGTCGAAACGATCGAGCACAACCGCGACAAGATGGTCGGCGTGACCGTGTTCATCGGCAAGAAGCGCGGCAAC
GCGAGCACGTCGGATTTTTCGCCGGGCGCGATCAAGGATACCGTCGCCGCCGCGTACAACATCGCACGCTTCACGGCCGA
GGACGAAGCGGCCGGCCTGGCCGAGGCCGAACTGCTCGAAACCGACCCGCGTGACCTCGACCTGTACCACCCGTGGGCGC
TGACGGCCGACGAGGCGGTCGAGCTCGCCCGCCGCGCGGAAGACGCCGCATTCGCGGTCAGCCCGCAGATCCGCAATTCG
GAAGGCGCAAGCGTGTCGGCCCAGCATTCGCAATTCGTGCTGGCCACGTCGCGCGGCTTCCTGTCCGGCTACCCGTACTC
GCGCCACTACATCGCATGCGCACCGATCGCGGGCAGCGGGCGTCACATGCAGCGCGACGACTGGTATTCGTCGAAGCGCA
GCGCGATCGATCTCGCGGCACCCGAAGCGGTAGGCCGTTACGCGGCCGAGCGTGCGCTGGCGCGGATGGGCGCACGCCGC
CTCGACACCCGCAAGGTGCCCGTGCTGTTCGAGGCGCCGCTCGCGGCCGGCCTGCTCGGCGCATTCGTGCAGGCGGTGAG
CGGCGGCGCGCTGTACCGCAAGACGTCGTTCCTCGTCGACAGCCTCGGCAAGCCGGTGTTCGCACCGCACATCCAGGTCG
TCGAGGATCCGCACGTGCCGCGCGCGATGGGCAGCGCGCCGTTCGACGAGGAAGGCGTGCGCACGCGTGCGCGCAGCGTC
GTCAAGGATGGCGTCGTCGAAGGCTACTTCCTGTCGACCTATTCGGCGCGCAAGCTCGGCACGCAGACCACCGGCAACGC
GGGCGGCTCGCACAACCTCGCGCTGCGCAGCTCGAACACGCAGGCAGGCGACGATTTCGACGCGATGCTGAAGAAGCTCG
GCACGGGCCTGTTGCTGACCGAGCTGATGGGGCAGGGCGTGAACTACGTGACGGGTGACTATTCGCGCGGTGCGGCGGGC
TTCTGGGTCGAGAACGGCGTAATCCAGTATCCGGTCGAGGAAATCACCGTCGCGAGCACGCTGCAGGAAATGTTCCGGCA
TATCGTTGCGATCGGCGCCGATTCGATCGTGCGCGGCACGAAGGAAACGGGCTCGGTGCTGATCGAGCAGATGACGATCG
CCGGGCAGTAA

Upstream 100 bases:

>100_bases
CCCCAAGCTTGTCGGTGCCCGGCCCCGGACCTTGCTATGATCGCGGGATACGCAACATTTTGAACATGCGGGCGCCCGCC
CGCCACCGGATATCTAGACG

Downstream 100 bases:

>100_bases
GCGGCGCGCGGCATCGGCCGCCATCAAACAAAAAGCCCGACCGGCATGAGCCGGTCGGGCTTTTTTCATGGCGCGTGATG
CGTCAGCCGCGCTTGCGCTC

Product: microcin-processing peptidase 1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MAANLDVQARYFPHTQDQLKEIASDILRHAKALGATDAATEISEGDGLSVSVRRGEVETIEHNRDKMVGVTVFIGKKRGN
ASTSDFSPGAIKDTVAAAYNIARFTAEDEAAGLAEAELLETDPRDLDLYHPWALTADEAVELARRAEDAAFAVSPQIRNS
EGASVSAQHSQFVLATSRGFLSGYPYSRHYIACAPIAGSGRHMQRDDWYSSKRSAIDLAAPEAVGRYAAERALARMGARR
LDTRKVPVLFEAPLAAGLLGAFVQAVSGGALYRKTSFLVDSLGKPVFAPHIQVVEDPHVPRAMGSAPFDEEGVRTRARSV
VKDGVVEGYFLSTYSARKLGTQTTGNAGGSHNLALRSSNTQAGDDFDAMLKKLGTGLLLTELMGQGVNYVTGDYSRGAAG
FWVENGVIQYPVEEITVASTLQEMFRHIVAIGADSIVRGTKETGSVLIEQMTIAGQ

Sequences:

>Translated_456_residues
MAANLDVQARYFPHTQDQLKEIASDILRHAKALGATDAATEISEGDGLSVSVRRGEVETIEHNRDKMVGVTVFIGKKRGN
ASTSDFSPGAIKDTVAAAYNIARFTAEDEAAGLAEAELLETDPRDLDLYHPWALTADEAVELARRAEDAAFAVSPQIRNS
EGASVSAQHSQFVLATSRGFLSGYPYSRHYIACAPIAGSGRHMQRDDWYSSKRSAIDLAAPEAVGRYAAERALARMGARR
LDTRKVPVLFEAPLAAGLLGAFVQAVSGGALYRKTSFLVDSLGKPVFAPHIQVVEDPHVPRAMGSAPFDEEGVRTRARSV
VKDGVVEGYFLSTYSARKLGTQTTGNAGGSHNLALRSSNTQAGDDFDAMLKKLGTGLLLTELMGQGVNYVTGDYSRGAAG
FWVENGVIQYPVEEITVASTLQEMFRHIVAIGADSIVRGTKETGSVLIEQMTIAGQ
>Mature_455_residues
AANLDVQARYFPHTQDQLKEIASDILRHAKALGATDAATEISEGDGLSVSVRRGEVETIEHNRDKMVGVTVFIGKKRGNA
STSDFSPGAIKDTVAAAYNIARFTAEDEAAGLAEAELLETDPRDLDLYHPWALTADEAVELARRAEDAAFAVSPQIRNSE
GASVSAQHSQFVLATSRGFLSGYPYSRHYIACAPIAGSGRHMQRDDWYSSKRSAIDLAAPEAVGRYAAERALARMGARRL
DTRKVPVLFEAPLAAGLLGAFVQAVSGGALYRKTSFLVDSLGKPVFAPHIQVVEDPHVPRAMGSAPFDEEGVRTRARSVV
KDGVVEGYFLSTYSARKLGTQTTGNAGGSHNLALRSSNTQAGDDFDAMLKKLGTGLLLTELMGQGVNYVTGDYSRGAAGF
WVENGVIQYPVEEITVASTLQEMFRHIVAIGADSIVRGTKETGSVLIEQMTIAGQ

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=439, Percent_Identity=47.8359908883827, Blast_Score=408, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48867; Mature: 48736

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAANLDVQARYFPHTQDQLKEIASDILRHAKALGATDAATEISEGDGLSVSVRRGEVETI
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEECCCCHHH
EHNRDKMVGVTVFIGKKRGNASTSDFSPGAIKDTVAAAYNIARFTAEDEAAGLAEAELLE
HCCCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHCCCHHHHHHC
TDPRDLDLYHPWALTADEAVELARRAEDAAFAVSPQIRNSEGASVSAQHSQFVLATSRGF
CCCCCCEEECCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCEEECCCCEEEEEECCCC
LSGYPYSRHYIACAPIAGSGRHMQRDDWYSSKRSAIDLAAPEAVGRYAAERALARMGARR
CCCCCCCCCEEEEECCCCCCCCCCCCHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHH
LDTRKVPVLFEAPLAAGLLGAFVQAVSGGALYRKTSFLVDSLGKPVFAPHIQVVEDPHVP
CCCCCCCEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCC
RAMGSAPFDEEGVRTRARSVVKDGVVEGYFLSTYSARKLGTQTTGNAGGSHNLALRSSNT
HHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHCCCCCCCCCCCCCCEEEECCCC
QAGDDFDAMLKKLGTGLLLTELMGQGVNYVTGDYSRGAAGFWVENGVIQYPVEEITVAST
CCCCCHHHHHHHHCCHHHHHHHHHCCCCEEECCCCCCCCCEEEECCEEECCHHHHHHHHH
LQEMFRHIVAIGADSIVRGTKETGSVLIEQMTIAGQ
HHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AANLDVQARYFPHTQDQLKEIASDILRHAKALGATDAATEISEGDGLSVSVRRGEVETI
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEECCCCHHH
EHNRDKMVGVTVFIGKKRGNASTSDFSPGAIKDTVAAAYNIARFTAEDEAAGLAEAELLE
HCCCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHCCCHHHHHHC
TDPRDLDLYHPWALTADEAVELARRAEDAAFAVSPQIRNSEGASVSAQHSQFVLATSRGF
CCCCCCEEECCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCEEECCCCEEEEEECCCC
LSGYPYSRHYIACAPIAGSGRHMQRDDWYSSKRSAIDLAAPEAVGRYAAERALARMGARR
CCCCCCCCCEEEEECCCCCCCCCCCCHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHH
LDTRKVPVLFEAPLAAGLLGAFVQAVSGGALYRKTSFLVDSLGKPVFAPHIQVVEDPHVP
CCCCCCCEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCC
RAMGSAPFDEEGVRTRARSVVKDGVVEGYFLSTYSARKLGTQTTGNAGGSHNLALRSSNT
HHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHCCCCCCCCCCCCCCEEEECCCC
QAGDDFDAMLKKLGTGLLLTELMGQGVNYVTGDYSRGAAGFWVENGVIQYPVEEITVAST
CCCCCHHHHHHHHCCHHHHHHHHHCCCCEEECCCCCCCCCEEEECCEEECCHHHHHHHHH
LQEMFRHIVAIGADSIVRGTKETGSVLIEQMTIAGQ
HHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]