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The map label for this gene is dadA [H]

Identifier: 78065650

GI number: 78065650

Start: 1114550

End: 1115836

Strand: Reverse

Name: dadA [H]

Synonym: Bcep18194_A4178

Alternate gene names: 78065650

Gene position: 1115836-1114550 (Counterclockwise)

Preceding gene: 78065653

Following gene: 78065643

Centisome position: 30.21

GC content: 66.59

Gene sequence:

>1287_bases
ATGCGAGTCGTCATTCTGGGCAGCGGTGTGGTGGGCGTGGCAAGCGCGTATTACCTCGCGCGCGCCGGTCATGAAGTCAC
GGTGATCGACCGGGAGGCCGGCCCGGCGCTCGAGACGAGCTTCGCGAACGCAGGCCAGATCTCCCCCGGCTATGCCGCGC
CGTGGGCCGCACCCGGCGTGCCGCTGAAGGCCGTCAAGTGGATGTTCGAAAAACACGCGCCGCTCGCGATCCGCCTCGAC
GGCACGCGCTTCCAGCTCCAGTGGATGTACCAGATGCTGCGCAACTGCACGGCCGAGCGCTATGCGGTCAACAAGGGCCG
CATGGTCCGCCTCGCCGAATACAGCCGCGACTGCCTGCAGGCACTGCGCGCCGATACCGGCATCCAGTACGAAGGCCGCA
CGGGCGGCACGCTGCAACTGTTCCGCACGCAGCAGCAACTCGACGGCGCGGCGAAGGACATCGCCGTCCTGCAGGAAGCG
AACGTGCCGTTCGAACTGCTGTCGCCGGCCGACCTGAAGAAAGCCGAACCGGCGCTTGCCGCCGTCTCGCACAAGCTGAC
GGGCGGCCTGCGCCTGCCGGGTGACGAAACGGGCGACTGCCAGCTGTTCACGACGCGCCTTGCAGCGCTCGCCGAATCGC
TTGGCGTGAAGTTCCGCTACAACACGCCGATCGATGCACTCGCAATCGCGGGCGGCAAGATCGCCGGCGTGCAATGCGGC
AGCGAGACGGTGCGCGCGGACGCTTACGTCGTCGCGCTCGGCTCGTACTCGACCAGCTTCATCTCGAACCTGATGAAGAT
CCCCGTCTATCCGCTGAAGGGCTATTCGATCACCGCGCCGATCGTCAACGAAGCGGCCGCGCCCGTGTCGACCGTGCTCG
ACGAAACCTACAAGATCGCAATCACGCGTTTCGACCAGCGCATCCGCGTCGGCGGCATGGCGGAAATCGTCGGCTTCGAC
AAGAAGCTACGTGCCGCGCGCCGCGAAACGCTCGAAATGTGCGTGAACGACCTGTTCCCGGGCGGCGGCGACACGTCGAA
GGCAACGTTCTGGACGGGCCTGCGCCCGATGACGCCGGACGGTACGCCGATCGTTGGCCGCACGCCGGTGTCGAACCTGT
TCCTGAACACCGGTCACGGCACGCTTGGCTGGACGATGTCGTGCGGCTCGGGCCAGCTGCTCGCCGACCTGATCTCGGGC
AAGATGCCCGCGATCCAGGCCGACGACCTGTCGGTGCATCGTTACCTGAAGGACGTGGCCGGCCAGACGCGTCCGGCATA
CGCATAA

Upstream 100 bases:

>100_bases
ATTCGACAATACCATAGCGCAAAAAACTAGATCAGCTAGAGGTTACACGGAGACGCAGGTGCGCCCGGCCTCGATCTGGT
CCCTTCCATCTGGAGCAGCT

Downstream 100 bases:

>100_bases
GCCGCGGGTGCCTGCAGCAATGCGGGCCAGCATGAAAAAGGCGCCCCTCGGGGCGCCTTTTTCGTTTCACCGGATGCGTG
GCGGAATCAGAACTGGTCTT

Product: D-amino acid dehydrogenase small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MRVVILGSGVVGVASAYYLARAGHEVTVIDREAGPALETSFANAGQISPGYAAPWAAPGVPLKAVKWMFEKHAPLAIRLD
GTRFQLQWMYQMLRNCTAERYAVNKGRMVRLAEYSRDCLQALRADTGIQYEGRTGGTLQLFRTQQQLDGAAKDIAVLQEA
NVPFELLSPADLKKAEPALAAVSHKLTGGLRLPGDETGDCQLFTTRLAALAESLGVKFRYNTPIDALAIAGGKIAGVQCG
SETVRADAYVVALGSYSTSFISNLMKIPVYPLKGYSITAPIVNEAAAPVSTVLDETYKIAITRFDQRIRVGGMAEIVGFD
KKLRAARRETLEMCVNDLFPGGGDTSKATFWTGLRPMTPDGTPIVGRTPVSNLFLNTGHGTLGWTMSCGSGQLLADLISG
KMPAIQADDLSVHRYLKDVAGQTRPAYA

Sequences:

>Translated_428_residues
MRVVILGSGVVGVASAYYLARAGHEVTVIDREAGPALETSFANAGQISPGYAAPWAAPGVPLKAVKWMFEKHAPLAIRLD
GTRFQLQWMYQMLRNCTAERYAVNKGRMVRLAEYSRDCLQALRADTGIQYEGRTGGTLQLFRTQQQLDGAAKDIAVLQEA
NVPFELLSPADLKKAEPALAAVSHKLTGGLRLPGDETGDCQLFTTRLAALAESLGVKFRYNTPIDALAIAGGKIAGVQCG
SETVRADAYVVALGSYSTSFISNLMKIPVYPLKGYSITAPIVNEAAAPVSTVLDETYKIAITRFDQRIRVGGMAEIVGFD
KKLRAARRETLEMCVNDLFPGGGDTSKATFWTGLRPMTPDGTPIVGRTPVSNLFLNTGHGTLGWTMSCGSGQLLADLISG
KMPAIQADDLSVHRYLKDVAGQTRPAYA
>Mature_428_residues
MRVVILGSGVVGVASAYYLARAGHEVTVIDREAGPALETSFANAGQISPGYAAPWAAPGVPLKAVKWMFEKHAPLAIRLD
GTRFQLQWMYQMLRNCTAERYAVNKGRMVRLAEYSRDCLQALRADTGIQYEGRTGGTLQLFRTQQQLDGAAKDIAVLQEA
NVPFELLSPADLKKAEPALAAVSHKLTGGLRLPGDETGDCQLFTTRLAALAESLGVKFRYNTPIDALAIAGGKIAGVQCG
SETVRADAYVVALGSYSTSFISNLMKIPVYPLKGYSITAPIVNEAAAPVSTVLDETYKIAITRFDQRIRVGGMAEIVGFD
KKLRAARRETLEMCVNDLFPGGGDTSKATFWTGLRPMTPDGTPIVGRTPVSNLFLNTGHGTLGWTMSCGSGQLLADLISG
KMPAIQADDLSVHRYLKDVAGQTRPAYA

Specific function: Oxidative deamination of D-amino acids [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=427, Percent_Identity=71.6627634660422, Blast_Score=645, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023080
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.4.99.1 [H]

Molecular weight: Translated: 45978; Mature: 45978

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVVILGSGVVGVASAYYLARAGHEVTVIDREAGPALETSFANAGQISPGYAAPWAAPGV
CEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHCCCCCCCCCCCCCCCCCCCCC
PLKAVKWMFEKHAPLAIRLDGTRFQLQWMYQMLRNCTAERYAVNKGRMVRLAEYSRDCLQ
CHHHHHHHHHCCCCEEEEECCCEEHHHHHHHHHHHCCHHHEECCCCCEEEHHHHHHHHHH
ALRADTGIQYEGRTGGTLQLFRTQQQLDGAAKDIAVLQEANVPFELLSPADLKKAEPALA
HHHHCCCCEECCCCCCEEEEEECHHHHCCHHHHHHHHHHCCCCCEECCCCCCCCCCHHHH
AVSHKLTGGLRLPGDETGDCQLFTTRLAALAESLGVKFRYNTPIDALAIAGGKIAGVQCG
HHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCHHHEEECCCEEEEEECC
SETVRADAYVVALGSYSTSFISNLMKIPVYPLKGYSITAPIVNEAAAPVSTVLDETYKIA
CCCEECCEEEEEECCCCHHHHHHHHHCCCEECCCCEEECCHHHHHCCHHHHHHCCHHEEE
ITRFDQRIRVGGMAEIVGFDKKLRAARRETLEMCVNDLFPGGGDTSKATFWTGLRPMTPD
EEECCCHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCC
GTPIVGRTPVSNLFLNTGHGTLGWTMSCGSGQLLADLISGKMPAIQADDLSVHRYLKDVA
CCEEECCCCCCCEEEECCCCCEEEEEECCCCHHHHHHHCCCCCCEECCCHHHHHHHHHHC
GQTRPAYA
CCCCCCCC
>Mature Secondary Structure
MRVVILGSGVVGVASAYYLARAGHEVTVIDREAGPALETSFANAGQISPGYAAPWAAPGV
CEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHCCCCCCCCCCCCCCCCCCCCC
PLKAVKWMFEKHAPLAIRLDGTRFQLQWMYQMLRNCTAERYAVNKGRMVRLAEYSRDCLQ
CHHHHHHHHHCCCCEEEEECCCEEHHHHHHHHHHHCCHHHEECCCCCEEEHHHHHHHHHH
ALRADTGIQYEGRTGGTLQLFRTQQQLDGAAKDIAVLQEANVPFELLSPADLKKAEPALA
HHHHCCCCEECCCCCCEEEEEECHHHHCCHHHHHHHHHHCCCCCEECCCCCCCCCCHHHH
AVSHKLTGGLRLPGDETGDCQLFTTRLAALAESLGVKFRYNTPIDALAIAGGKIAGVQCG
HHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCHHHEEECCCEEEEEECC
SETVRADAYVVALGSYSTSFISNLMKIPVYPLKGYSITAPIVNEAAAPVSTVLDETYKIA
CCCEECCEEEEEECCCCHHHHHHHHHCCCEECCCCEEECCHHHHHCCHHHHHHCCHHEEE
ITRFDQRIRVGGMAEIVGFDKKLRAARRETLEMCVNDLFPGGGDTSKATFWTGLRPMTPD
EEECCCHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCC
GTPIVGRTPVSNLFLNTGHGTLGWTMSCGSGQLLADLISGKMPAIQADDLSVHRYLKDVA
CCEEECCCCCCCEEEECCCCCEEEEEECCCCHHHHHHHCCCCCCEECCCHHHHHHHHHHC
GQTRPAYA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA