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The map label for this gene is rph

Identifier: 78065577

GI number: 78065577

Start: 1034001

End: 1034732

Strand: Reverse

Name: rph

Synonym: Bcep18194_A4105

Alternate gene names: 78065577

Gene position: 1034732-1034001 (Counterclockwise)

Preceding gene: 78065583

Following gene: 78065576

Centisome position: 28.01

GC content: 69.81

Gene sequence:

>732_bases
ATGACGTCCTCCGTTTCCCGCCCGAGCGGCCGCCGCGCCGACGCACTGCGCAAGGTCGCCCTTACGCGCCACTACACGAA
ACATGCCGAAGGCTCGGTGCTGGTCGAATTCGGCGACACCAAGGTGCTCTGCACCGCGAGCGTCTCCGAACGCGTCCCCG
ACTTCCTGCGCGACCGCGGGCAAGGCTGGCTGACCGCCGAATACGGGATGCTGCCGCGCGCGACGCACACGCGCAGCGAC
CGCGAAGCCGCCCGCGGCAAGCAGACCGGCCGCACGCAGGAAATTCAGCGCCTGATCGGCCGCGCGCTGCGCGCGGTGTT
CGACCTCGAGGCGCTCGGCCCGCGCACGATCCACATCGACTGCGACGTGATCCAGGCCGACGGCGGCACGCGCACGGCGA
GCATCACCGGCGCGTTTGTGGCCGCGCACGACGCCGTGTCGAAGCTGATCGCGGCCGGCAAGATCACGCGCTCGCCGATC
ACCGACCACGTCGCCGCGATCTCGGTCGGCGTGTACGAAGGCGCGCCCGTGCTCGACCTCGATTACGCGGAAGATTCGCA
GTGCGACACCGACATGAACGTCGTGATGACCGGCGCCGGCGGCTTCGTCGAAGTCCAGGGCACGGCCGAAGGCGTGCCGT
TCTCGCGCGCCGAGATGAATGCGCTGCTCGACCTCGCGCAAGGCGGGATCGCCGAGCTCGTGCAGCTCCAGAAAGACGTG
CTGGGCGCCTGA

Upstream 100 bases:

>100_bases
GCGGGAAGCGCGCATTATCCCATTTTTGCGGACCATCCCGCCGGGCACACCCGCCGTTCGGCGCTAAAATCGGGGTTTCC
TCTCGTTCCGCGACTCCCAC

Downstream 100 bases:

>100_bases
CATGCCGGACGACCACACCATCGCGCCGCTGTCGCGCATCGTTCTCGCGTCGAACAACGCCGGCAAGCTGCGCGAATTCA
CCGCGCTGTTCTCGACGGTC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MTSSVSRPSGRRADALRKVALTRHYTKHAEGSVLVEFGDTKVLCTASVSERVPDFLRDRGQGWLTAEYGMLPRATHTRSD
REAARGKQTGRTQEIQRLIGRALRAVFDLEALGPRTIHIDCDVIQADGGTRTASITGAFVAAHDAVSKLIAAGKITRSPI
TDHVAAISVGVYEGAPVLDLDYAEDSQCDTDMNVVMTGAGGFVEVQGTAEGVPFSRAEMNALLDLAQGGIAELVQLQKDV
LGA

Sequences:

>Translated_243_residues
MTSSVSRPSGRRADALRKVALTRHYTKHAEGSVLVEFGDTKVLCTASVSERVPDFLRDRGQGWLTAEYGMLPRATHTRSD
REAARGKQTGRTQEIQRLIGRALRAVFDLEALGPRTIHIDCDVIQADGGTRTASITGAFVAAHDAVSKLIAAGKITRSPI
TDHVAAISVGVYEGAPVLDLDYAEDSQCDTDMNVVMTGAGGFVEVQGTAEGVPFSRAEMNALLDLAQGGIAELVQLQKDV
LGA
>Mature_242_residues
TSSVSRPSGRRADALRKVALTRHYTKHAEGSVLVEFGDTKVLCTASVSERVPDFLRDRGQGWLTAEYGMLPRATHTRSDR
EAARGKQTGRTQEIQRLIGRALRAVFDLEALGPRTIHIDCDVIQADGGTRTASITGAFVAAHDAVSKLIAAGKITRSPIT
DHVAAISVGVYEGAPVLDLDYAEDSQCDTDMNVVMTGAGGFVEVQGTAEGVPFSRAEMNALLDLAQGGIAELVQLQKDVL
GA

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=209, Percent_Identity=67.9425837320574, Blast_Score=291, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI71981632, Length=186, Percent_Identity=29.0322580645161, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 25891; Mature: 25759

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSVSRPSGRRADALRKVALTRHYTKHAEGSVLVEFGDTKVLCTASVSERVPDFLRDRG
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCHHHHCHHHHHHCC
QGWLTAEYGMLPRATHTRSDREAARGKQTGRTQEIQRLIGRALRAVFDLEALGPRTIHID
CCEEEECCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
CDVIQADGGTRTASITGAFVAAHDAVSKLIAAGKITRSPITDHVAAISVGVYEGAPVLDL
EEEEECCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHEEEEEECCCCEEEE
DYAEDSQCDTDMNVVMTGAGGFVEVQGTAEGVPFSRAEMNALLDLAQGGIAELVQLQKDV
CCCCCCCCCCCCEEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
LGA
HCC
>Mature Secondary Structure 
TSSVSRPSGRRADALRKVALTRHYTKHAEGSVLVEFGDTKVLCTASVSERVPDFLRDRG
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCHHHHCHHHHHHCC
QGWLTAEYGMLPRATHTRSDREAARGKQTGRTQEIQRLIGRALRAVFDLEALGPRTIHID
CCEEEECCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
CDVIQADGGTRTASITGAFVAAHDAVSKLIAAGKITRSPITDHVAAISVGVYEGAPVLDL
EEEEECCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHEEEEEECCCCEEEE
DYAEDSQCDTDMNVVMTGAGGFVEVQGTAEGVPFSRAEMNALLDLAQGGIAELVQLQKDV
CCCCCCCCCCCCEEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
LGA
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA