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The map label for this gene is glk [H]

Identifier: 78065540

GI number: 78065540

Start: 993582

End: 995510

Strand: Reverse

Name: glk [H]

Synonym: Bcep18194_A4068

Alternate gene names: 78065540

Gene position: 995510-993582 (Counterclockwise)

Preceding gene: 78065541

Following gene: 78065538

Centisome position: 26.95

GC content: 68.33

Gene sequence:

>1929_bases
ATGTCTACTGGTGCGCAAAGTAAGGCGGCCGTCGCAGGCCAGCATGCCGACGGCCCGCGCCTGCTCGCGGACGTCGGCGG
CACCAACGCGCGCTTCGCGCTGGAAACCGGCCCGGGCGACATCACGCAGATCCGCGTGTATCCCGGCGCCGACTATCCGA
CGCTCACCGACGCGATCCGCAAATACCTGAAGGACGTGAAGATCACCCGCGTGAACCACGCGGCGATCGCGATCGCCAAT
CCGGTCGACGGCGACCAGGTCACGATGACCAACCACGACTGGAGCTTCTCGATCGAGGCGACGCGCCGCGCGCTCGGCTT
CGACACGCTGCTCGTCGTCAACGATTTCACCGCGCTCGCGATGGCGCTGCCGGGCCTGACCGACGCGCAGCGCGTGCAGA
TCGGCGGCGGCGCGCGCCGCCAGAACAGCGTGATCGGGCTGCTCGGGCCCGGCACCGGGCTCGGCGTGTCGGGGCTGATT
CCGGCCGACGACCGCTGGATCGCGCTCGGCAGCGAAGGCGGCCACGCATCGTTCGCACCGCAGGACGAGCGCGAGGACCT
GGTGCTGCAGTACGCGCGCAAGAAGTTTCCGCACGTGTCGTTCGAACGCGTGTGCGCGGGCCCCGGCATGGAGATCATCT
ATCGCGCGCTCGCCGCGCGCGACAAGAAGCGCGTGGCCGCGACCGTCGACACGGTCGAGATCGTCGAACGCGCGCATGCG
GGCGACGCACTCGCGCTCGAGACGGTTGAATGCTTCTGCGGAATTCTCGGCGCGTTCGCGGGCAGCGTCGCGCTGACGCT
CGGCGCGCTCGGCGGCGTGTACATCGGCGGCGGTGTCGCGCTGAAGCTCGGCGAACTGTTCACGCGCTCGTCGTTCCGCG
CGCGCTTCGAGGCAAAGGGCCGCTTCACGCACTACCTCGAGAACATCCCGACCTACCTGATCACCGCCGAATACCCGGCG
TTCCTCGGCGTATCGGCGATCCTCGCGGAGCAGCTGTCGAACCGCTCGGGCGGTGCGTCGTCGGCCGTGTTCGAGCGGAT
CCGCCAGATGCGCGACGCACTGACGCCGGCCGAGCGCCGCGTCGCCGATCTCGCACTGAACCACCCGCGCTCGATCATCA
ACGATCCGATCGTCGACATCGCACGCAAGGCCGACGTGAGCCAGCCGACCGTGATCCGCTTCTGCCGCTCGCTCGGCTGC
CAGGGGCTGTCGGACTTCAAGCTGAAGCTCGCCACCGGCCTCACCGGCACGATCCCGATGAGCCACAGCCAGGTGCATCT
CGGCGACACGGCCACCGACTTCGGCGCGAAGGTGCTCGACAACACGGTGTCCGCGATCCTGCAGTTGCGCGAGCACCTGA
ATTTCGAGCACGTCGAGAACGCGATCGAGATCCTGAACGGCGCGCGGCGCATCGAGTTCTACGGGCTCGGCAACTCGAAC
ATCGTCGCGCAGGACGCGCATTACAAGTTCTTCCGCTTCGGGATCCCGACGATCGCGTACGGCGACCTGTACATGCAGGC
CGCATCGGCCGCGCTGCTCGGCAAGGGCGACGTGATCGTCGCGGTGTCGAAGTCGGGGCGCGCGCCCGAGCTGCTGCGCG
TGCTCGACGTCGCGATGCAGGCCGGCGCGAAGGTGATCGCGATCACGTCGAGCAACACGCCGCTCGCAAAGCGCGCGACC
GTCGCGCTCGAAACCGACCACATCGAGATGCGCGAGTCGCAGCTGTCGATGATCTCGCGGATCCTGCATCTGCTGATGAT
CGACATCCTCGCGGTGGGCGTCGCGATCCGTCGTGCATCGACGAACGGCGAACTGCCCGAGGCCGTCGCGCAGGCGAAGG
CCCGTGCGAGCGACGACGAAACGGCCGACGTGCTCGACTGGCTGAGCCACGGTGCATCGCCGGCGGCGAAGGACGTCGCG
CGGGATTGA

Upstream 100 bases:

>100_bases
ATCGCGGGCAACGCGAAGCGCGCGGTGCTCGACGCCGCAGCCGCATCGTTGCAGAAGAACGCGATTTCCCAACTGGCCAA
CGACAAGGGGACCCAGCTCG

Downstream 100 bases:

>100_bases
CGCGGTGCGGCGGCAGCCGCTTCCCGCGTTCGAAACAAAAACGCCACCGGCGCAGCGCGCGCGGTGGCGTTTTTCATTTT
GCCGTCAGGCCGCGGTTCAG

Product: bifunctional glucokinase/RpiR family transcriptional regulator

Products: NA

Alternate protein names: Glucokinase; Glucose kinase; Putative HTH-type transcriptional regulator [H]

Number of amino acids: Translated: 642; Mature: 641

Protein sequence:

>642_residues
MSTGAQSKAAVAGQHADGPRLLADVGGTNARFALETGPGDITQIRVYPGADYPTLTDAIRKYLKDVKITRVNHAAIAIAN
PVDGDQVTMTNHDWSFSIEATRRALGFDTLLVVNDFTALAMALPGLTDAQRVQIGGGARRQNSVIGLLGPGTGLGVSGLI
PADDRWIALGSEGGHASFAPQDEREDLVLQYARKKFPHVSFERVCAGPGMEIIYRALAARDKKRVAATVDTVEIVERAHA
GDALALETVECFCGILGAFAGSVALTLGALGGVYIGGGVALKLGELFTRSSFRARFEAKGRFTHYLENIPTYLITAEYPA
FLGVSAILAEQLSNRSGGASSAVFERIRQMRDALTPAERRVADLALNHPRSIINDPIVDIARKADVSQPTVIRFCRSLGC
QGLSDFKLKLATGLTGTIPMSHSQVHLGDTATDFGAKVLDNTVSAILQLREHLNFEHVENAIEILNGARRIEFYGLGNSN
IVAQDAHYKFFRFGIPTIAYGDLYMQAASAALLGKGDVIVAVSKSGRAPELLRVLDVAMQAGAKVIAITSSNTPLAKRAT
VALETDHIEMRESQLSMISRILHLLMIDILAVGVAIRRASTNGELPEAVAQAKARASDDETADVLDWLSHGASPAAKDVA
RD

Sequences:

>Translated_642_residues
MSTGAQSKAAVAGQHADGPRLLADVGGTNARFALETGPGDITQIRVYPGADYPTLTDAIRKYLKDVKITRVNHAAIAIAN
PVDGDQVTMTNHDWSFSIEATRRALGFDTLLVVNDFTALAMALPGLTDAQRVQIGGGARRQNSVIGLLGPGTGLGVSGLI
PADDRWIALGSEGGHASFAPQDEREDLVLQYARKKFPHVSFERVCAGPGMEIIYRALAARDKKRVAATVDTVEIVERAHA
GDALALETVECFCGILGAFAGSVALTLGALGGVYIGGGVALKLGELFTRSSFRARFEAKGRFTHYLENIPTYLITAEYPA
FLGVSAILAEQLSNRSGGASSAVFERIRQMRDALTPAERRVADLALNHPRSIINDPIVDIARKADVSQPTVIRFCRSLGC
QGLSDFKLKLATGLTGTIPMSHSQVHLGDTATDFGAKVLDNTVSAILQLREHLNFEHVENAIEILNGARRIEFYGLGNSN
IVAQDAHYKFFRFGIPTIAYGDLYMQAASAALLGKGDVIVAVSKSGRAPELLRVLDVAMQAGAKVIAITSSNTPLAKRAT
VALETDHIEMRESQLSMISRILHLLMIDILAVGVAIRRASTNGELPEAVAQAKARASDDETADVLDWLSHGASPAAKDVA
RD
>Mature_641_residues
STGAQSKAAVAGQHADGPRLLADVGGTNARFALETGPGDITQIRVYPGADYPTLTDAIRKYLKDVKITRVNHAAIAIANP
VDGDQVTMTNHDWSFSIEATRRALGFDTLLVVNDFTALAMALPGLTDAQRVQIGGGARRQNSVIGLLGPGTGLGVSGLIP
ADDRWIALGSEGGHASFAPQDEREDLVLQYARKKFPHVSFERVCAGPGMEIIYRALAARDKKRVAATVDTVEIVERAHAG
DALALETVECFCGILGAFAGSVALTLGALGGVYIGGGVALKLGELFTRSSFRARFEAKGRFTHYLENIPTYLITAEYPAF
LGVSAILAEQLSNRSGGASSAVFERIRQMRDALTPAERRVADLALNHPRSIINDPIVDIARKADVSQPTVIRFCRSLGCQ
GLSDFKLKLATGLTGTIPMSHSQVHLGDTATDFGAKVLDNTVSAILQLREHLNFEHVENAIEILNGARRIEFYGLGNSNI
VAQDAHYKFFRFGIPTIAYGDLYMQAASAALLGKGDVIVAVSKSGRAPELLRVLDVAMQAGAKVIAITSSNTPLAKRATV
ALETDHIEMRESQLSMISRILHLLMIDILAVGVAIRRASTNGELPEAVAQAKARASDDETADVLDWLSHGASPAAKDVAR
D

Specific function: Not Highly Important In E.Coli As Glucose Is Transported Into The Cell By The Pts System Already As Glucose 6-Phosphate. [C]

COG id: COG0837

COG function: function code G; Glucokinase

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SIS domain [H]

Homologues:

Organism=Escherichia coli, GI1788732, Length=321, Percent_Identity=44.5482866043614, Blast_Score=269, Evalue=3e-73,
Organism=Escherichia coli, GI1788159, Length=256, Percent_Identity=36.71875, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI87082369, Length=291, Percent_Identity=27.1477663230241, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788767, Length=249, Percent_Identity=26.9076305220884, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI87082130, Length=226, Percent_Identity=28.7610619469027, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003836
- InterPro:   IPR000843
- InterPro:   IPR000281
- InterPro:   IPR001347
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02685 Glucokinase; PF01418 HTH_6; PF01380 SIS [H]

EC number: =2.7.1.2 [H]

Molecular weight: Translated: 68508; Mature: 68377

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00356 HTH_LACI_1 ; PS51071 HTH_RPIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTGAQSKAAVAGQHADGPRLLADVGGTNARFALETGPGDITQIRVYPGADYPTLTDAIR
CCCCCCCCHHHCCCCCCCCEEEEECCCCCCEEEEECCCCCEEEEEEECCCCCCHHHHHHH
KYLKDVKITRVNHAAIAIANPVDGDQVTMTNHDWSFSIEATRRALGFDTLLVVNDFTALA
HHHHCCEEEEECCEEEEEECCCCCCEEEEECCCEEEEHHHHHHHHCCCEEEEHHHHHHHH
MALPGLTDAQRVQIGGGARRQNSVIGLLGPGTGLGVSGLIPADDRWIALGSEGGHASFAP
HHCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCCCCC
QDEREDLVLQYARKKFPHVSFERVCAGPGMEIIYRALAARDKKRVAATVDTVEIVERAHA
CCCHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
GDALALETVECFCGILGAFAGSVALTLGALGGVYIGGGVALKLGELFTRSSFRARFEAKG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCEEHHHHHHHHHHHHHHHHCCC
RFTHYLENIPTYLITAEYPAFLGVSAILAEQLSNRSGGASSAVFERIRQMRDALTPAERR
HHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHH
VADLALNHPRSIINDPIVDIARKADVSQPTVIRFCRSLGCQGLSDFKLKLATGLTGTIPM
HHHHHHCCCHHHHCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCC
SHSQVHLGDTATDFGAKVLDNTVSAILQLREHLNFEHVENAIEILNGARRIEFYGLGNSN
CCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEECCCCC
IVAQDAHYKFFRFGIPTIAYGDLYMQAASAALLGKGDVIVAVSKSGRAPELLRVLDVAMQ
EEEECCCCEEEEECCCCEEEHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHH
AGAKVIAITSSNTPLAKRATVALETDHIEMRESQLSMISRILHLLMIDILAVGVAIRRAS
CCCEEEEEECCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
TNGELPEAVAQAKARASDDETADVLDWLSHGASPAAKDVARD
CCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHCCC
>Mature Secondary Structure 
STGAQSKAAVAGQHADGPRLLADVGGTNARFALETGPGDITQIRVYPGADYPTLTDAIR
CCCCCCCHHHCCCCCCCCEEEEECCCCCCEEEEECCCCCEEEEEEECCCCCCHHHHHHH
KYLKDVKITRVNHAAIAIANPVDGDQVTMTNHDWSFSIEATRRALGFDTLLVVNDFTALA
HHHHCCEEEEECCEEEEEECCCCCCEEEEECCCEEEEHHHHHHHHCCCEEEEHHHHHHHH
MALPGLTDAQRVQIGGGARRQNSVIGLLGPGTGLGVSGLIPADDRWIALGSEGGHASFAP
HHCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCCCCC
QDEREDLVLQYARKKFPHVSFERVCAGPGMEIIYRALAARDKKRVAATVDTVEIVERAHA
CCCHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
GDALALETVECFCGILGAFAGSVALTLGALGGVYIGGGVALKLGELFTRSSFRARFEAKG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCEEHHHHHHHHHHHHHHHHCCC
RFTHYLENIPTYLITAEYPAFLGVSAILAEQLSNRSGGASSAVFERIRQMRDALTPAERR
HHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHH
VADLALNHPRSIINDPIVDIARKADVSQPTVIRFCRSLGCQGLSDFKLKLATGLTGTIPM
HHHHHHCCCHHHHCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCC
SHSQVHLGDTATDFGAKVLDNTVSAILQLREHLNFEHVENAIEILNGARRIEFYGLGNSN
CCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEECCCCC
IVAQDAHYKFFRFGIPTIAYGDLYMQAASAALLGKGDVIVAVSKSGRAPELLRVLDVAMQ
EEEECCCCEEEEECCCCEEEHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHH
AGAKVIAITSSNTPLAKRATVALETDHIEMRESQLSMISRILHLLMIDILAVGVAIRRAS
CCCEEEEEECCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
TNGELPEAVAQAKARASDDETADVLDWLSHGASPAAKDVARD
CCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA