| Definition | Burkholderia sp. 383 chromosome 1, complete genome. |
|---|---|
| Accession | NC_007510 |
| Length | 3,694,126 |
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The map label for this gene is epsC [H]
Identifier: 78065458
GI number: 78065458
Start: 909269
End: 911152
Strand: Direct
Name: epsC [H]
Synonym: Bcep18194_A3984
Alternate gene names: 78065458
Gene position: 909269-911152 (Clockwise)
Preceding gene: 78065457
Following gene: 78065464
Centisome position: 24.61
GC content: 64.65
Gene sequence:
>1884_bases ATGTTGCGATCCAAAGCATCATGGCTGTCACTCAGTGCTTTCCTGTTCGATCTGCTGGCGGTTGTCGTCGCGTGGTTGTT CGCCTATCTTGTTCGCTTCAACGGTAGCGTTCCGCCCGATTTCCTGAGGGGTTCGCTGACTGCGCTCGCCTGGGTGCTTC CCGTCTATGCGCTGACGTTCCACCTGTTCGGCCTGTATCGCGGACTGTGGGTGTTCGCGAGCCTGCCGGACCTGGTGCGG ATTTCGAAGGCGCTTGCGGGCGGCGGCGTGATTGTGATGATCGGCGCCGTGATGTTCCAGCCGTCGCCGATCATCCCGCG TTCCGTGCTGCTGGTCTCCCCGCTGATGCTGTTCCTGATGATGGGCGGCGCTCGTGCGCTGTATCGCGCGATGAAGGAGT TTTACCTGTACGGCGGCCTCGTCGGGCAGGGCAAGCCGGTGCTCGTGATCGGCGCGGGCACGGCAGGCGCGAACCTGGCG CGCGAATTGTCGCGCTCGGGCGAATGGCGTCTCGTCGGCCTGCTGGATGACGACCAGACGAAGCAGGGCCGCGAAATCTA CGGCTACAAGGTGCTCGGCTCGCTCGACGACGTCGCGCACTGGACCGAGGCACTGAAGATCGAATACGCGATCGTGGCGA TTCCGTCCGCGTCGGTCGAGGTGCAGCGCCGGACGGCGACCCTCTGCGTGCGCGCCGGCTTGAAAGTGATGGTGCTGCCG TCGCTGACCGCACTGATGCCGGGGCAGGGCTTCCTGTCCCAGATCCGTGAGATCGACCTCGAGGATCTGCTCGGCCGCGA CGCCGTGACGATCGACACGCCGCACGTCGAGGCGCTGCTTCGCGGTCGTGTCGTGATGGTGACGGGCGCTGGCGGCTCGA TCGGTTCCGAGCTATGCCGGCAGATCCTGCGTTTTGCGCCAGCGCAACTCGTCGCGTTCGACCTGTCCGAGTACGCGATG TACCGCCTGACCGAGGAACTGCGCGAGCGCTTCCCCGATCAGCCGGTCGTGCCGATCATCGGCGACGCGAAGGATTCTCT GCTGCTCGATCAGGTGATGTCCCGCCACGTGCCGCATATCGTGTTTCATGCGGCGGCATACAAGCACGTGCCGCTGATGG AGGAGCACAACGCATGGCAGGCGCTGCGCAACAACGTGCTCGGCACGTATCGCGTGGCCCGTGCGGCGATCCGCCACGAC GTGCGTCACTTCGTGCTGATCTCGACCGACAAGGCGGTCAATCCGACCAACGTGATGGGCGCGAGCAAGCGCCTCGCGGA AATGGCCTGCCAGGCGTTGCAGCAGACGAGCGGGCGCACTCAATTCGAAACCGTGCGTTTCGGCAACGTGCTGGGCAGCG CGGGCAGCGTGATTCCGAAGTTCCAGCAGCAGATCGCGAAGGGCGGCCCGGTGACGGTCACGCATCCGCAGATCACGCGC TTCTTCATGACGATCCCGGAGGCGTCGCAACTCGTGCTGCAGGCGTCGAGCATGGGGCACGGCGGAGAGATCTTCATTCT CGACATGGGCGAGCCGGTGAAGATCGTCGATCTCGCATGCGACCTGATCCGCCTGTACGGTTTCTCGGAAGATCAGATCC AGATCGAGTTCACCGGGCTGCGGCCCGGCGAGAAGCTCTACGAGGAGCTGCTCGCGGACGACGAGACAACGACGCGCACC CCGCATCCGAAACTGCGGATCGCGCGTGCGCGGGAAGTACCGGACAATTTCCTCGACGAGCTGCTGCCGTGGCTGATGCA GCATCGTGTCCTGCCCGATGACGAAGTGCGGCGAGACCTGCGGCGCTGGGTGCCGGAATACCAAACGGCCGTGCCCCCGA CGCTGCAGAGCGTGCCATCGGTGCGCGTCGTATCGAACGGGTAA
Upstream 100 bases:
>100_bases GGCGTGGTATGGCGTGCTGATGTGTATCGGCGTGGCGATCGACATGCGCTGGCGCCGGCTGCGGACGGTCATTGATAACA ATTCGTGAGGTCTCTCGCCG
Downstream 100 bases:
>100_bases CTTGGCCGGGCGGCGGTTCGAACCGCATGGCAATGACGAAAAAAAACGCCCTACGGGGCGTTTTTTTGATGATGCCGGCG GGCCGTTCAGTGGCTGCGCC
Product: polysaccharide biosynthesis protein CapD
Products: UDPglucoseal [C]
Alternate protein names: NA
Number of amino acids: Translated: 627; Mature: 627
Protein sequence:
>627_residues MLRSKASWLSLSAFLFDLLAVVVAWLFAYLVRFNGSVPPDFLRGSLTALAWVLPVYALTFHLFGLYRGLWVFASLPDLVR ISKALAGGGVIVMIGAVMFQPSPIIPRSVLLVSPLMLFLMMGGARALYRAMKEFYLYGGLVGQGKPVLVIGAGTAGANLA RELSRSGEWRLVGLLDDDQTKQGREIYGYKVLGSLDDVAHWTEALKIEYAIVAIPSASVEVQRRTATLCVRAGLKVMVLP SLTALMPGQGFLSQIREIDLEDLLGRDAVTIDTPHVEALLRGRVVMVTGAGGSIGSELCRQILRFAPAQLVAFDLSEYAM YRLTEELRERFPDQPVVPIIGDAKDSLLLDQVMSRHVPHIVFHAAAYKHVPLMEEHNAWQALRNNVLGTYRVARAAIRHD VRHFVLISTDKAVNPTNVMGASKRLAEMACQALQQTSGRTQFETVRFGNVLGSAGSVIPKFQQQIAKGGPVTVTHPQITR FFMTIPEASQLVLQASSMGHGGEIFILDMGEPVKIVDLACDLIRLYGFSEDQIQIEFTGLRPGEKLYEELLADDETTTRT PHPKLRIARAREVPDNFLDELLPWLMQHRVLPDDEVRRDLRRWVPEYQTAVPPTLQSVPSVRVVSNG
Sequences:
>Translated_627_residues MLRSKASWLSLSAFLFDLLAVVVAWLFAYLVRFNGSVPPDFLRGSLTALAWVLPVYALTFHLFGLYRGLWVFASLPDLVR ISKALAGGGVIVMIGAVMFQPSPIIPRSVLLVSPLMLFLMMGGARALYRAMKEFYLYGGLVGQGKPVLVIGAGTAGANLA RELSRSGEWRLVGLLDDDQTKQGREIYGYKVLGSLDDVAHWTEALKIEYAIVAIPSASVEVQRRTATLCVRAGLKVMVLP SLTALMPGQGFLSQIREIDLEDLLGRDAVTIDTPHVEALLRGRVVMVTGAGGSIGSELCRQILRFAPAQLVAFDLSEYAM YRLTEELRERFPDQPVVPIIGDAKDSLLLDQVMSRHVPHIVFHAAAYKHVPLMEEHNAWQALRNNVLGTYRVARAAIRHD VRHFVLISTDKAVNPTNVMGASKRLAEMACQALQQTSGRTQFETVRFGNVLGSAGSVIPKFQQQIAKGGPVTVTHPQITR FFMTIPEASQLVLQASSMGHGGEIFILDMGEPVKIVDLACDLIRLYGFSEDQIQIEFTGLRPGEKLYEELLADDETTTRT PHPKLRIARAREVPDNFLDELLPWLMQHRVLPDDEVRRDLRRWVPEYQTAVPPTLQSVPSVRVVSNG >Mature_627_residues MLRSKASWLSLSAFLFDLLAVVVAWLFAYLVRFNGSVPPDFLRGSLTALAWVLPVYALTFHLFGLYRGLWVFASLPDLVR ISKALAGGGVIVMIGAVMFQPSPIIPRSVLLVSPLMLFLMMGGARALYRAMKEFYLYGGLVGQGKPVLVIGAGTAGANLA RELSRSGEWRLVGLLDDDQTKQGREIYGYKVLGSLDDVAHWTEALKIEYAIVAIPSASVEVQRRTATLCVRAGLKVMVLP SLTALMPGQGFLSQIREIDLEDLLGRDAVTIDTPHVEALLRGRVVMVTGAGGSIGSELCRQILRFAPAQLVAFDLSEYAM YRLTEELRERFPDQPVVPIIGDAKDSLLLDQVMSRHVPHIVFHAAAYKHVPLMEEHNAWQALRNNVLGTYRVARAAIRHD VRHFVLISTDKAVNPTNVMGASKRLAEMACQALQQTSGRTQFETVRFGNVLGSAGSVIPKFQQQIAKGGPVTVTHPQITR FFMTIPEASQLVLQASSMGHGGEIFILDMGEPVKIVDLACDLIRLYGFSEDQIQIEFTGLRPGEKLYEELLADDETTTRT PHPKLRIARAREVPDNFLDELLPWLMQHRVLPDDEVRRDLRRWVPEYQTAVPPTLQSVPSVRVVSNG
Specific function: Involved in biofilm formation [H]
COG id: COG1086
COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases
Gene ontology:
Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the polysaccharide synthase family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR016040 - InterPro: IPR003869 [H]
Pfam domain/function: PF02719 Polysacc_synt_2 [H]
EC number: 5.1.3.2 [C]
Molecular weight: Translated: 69396; Mature: 69396
Theoretical pI: Translated: 8.03; Mature: 8.03
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.6 %Cys (Translated Protein) 3.0 %Met (Translated Protein) 3.7 %Cys+Met (Translated Protein) 0.6 %Cys (Mature Protein) 3.0 %Met (Mature Protein) 3.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MLRSKASWLSLSAFLFDLLAVVVAWLFAYLVRFNGSVPPDFLRGSLTALAWVLPVYALTF CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH HLFGLYRGLWVFASLPDLVRISKALAGGGVIVMIGAVMFQPSPIIPRSVLLVSPLMLFLM HHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHEEHHHHHCCCCCCCCHHHHHHHHHHHHHH MGGARALYRAMKEFYLYGGLVGQGKPVLVIGAGTAGANLARELSRSGEWRLVGLLDDDQT HCCHHHHHHHHHHHHHHCCEECCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEEECCCCC KQGREIYGYKVLGSLDDVAHWTEALKIEYAIVAIPSASVEVQRRTATLCVRAGLKVMVLP CCCCEEECEEEECCHHHHHHHHHHHEEEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEC SLTALMPGQGFLSQIREIDLEDLLGRDAVTIDTPHVEALLRGRVVMVTGAGGSIGSELCR CHHHHCCCHHHHHHHHHCCHHHHCCCCCEEECCHHHHHHHCCCEEEEECCCCCHHHHHHH QILRFAPAQLVAFDLSEYAMYRLTEELRERFPDQPVVPIIGDAKDSLLLDQVMSRHVPHI HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHCCCCHH VFHAAAYKHVPLMEEHNAWQALRNNVLGTYRVARAAIRHDVRHFVLISTDKAVNPTNVMG HHHHHHHHCCCCHHHCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHH ASKRLAEMACQALQQTSGRTQFETVRFGNVLGSAGSVIPKFQQQIAKGGPVTVTHPQITR HHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEECHHHHH FFMTIPEASQLVLQASSMGHGGEIFILDMGEPVKIVDLACDLIRLYGFSEDQIQIEFTGL HHHCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEECC RPGEKLYEELLADDETTTRTPHPKLRIARAREVPDNFLDELLPWLMQHRVLPDDEVRRDL CCHHHHHHHHHCCCCCCCCCCCCCEEEHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHH RRWVPEYQTAVPPTLQSVPSVRVVSNG HHHCCHHHHCCCCHHHHCCCEEEECCC >Mature Secondary Structure MLRSKASWLSLSAFLFDLLAVVVAWLFAYLVRFNGSVPPDFLRGSLTALAWVLPVYALTF CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH HLFGLYRGLWVFASLPDLVRISKALAGGGVIVMIGAVMFQPSPIIPRSVLLVSPLMLFLM HHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHEEHHHHHCCCCCCCCHHHHHHHHHHHHHH MGGARALYRAMKEFYLYGGLVGQGKPVLVIGAGTAGANLARELSRSGEWRLVGLLDDDQT HCCHHHHHHHHHHHHHHCCEECCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEEECCCCC KQGREIYGYKVLGSLDDVAHWTEALKIEYAIVAIPSASVEVQRRTATLCVRAGLKVMVLP CCCCEEECEEEECCHHHHHHHHHHHEEEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEC SLTALMPGQGFLSQIREIDLEDLLGRDAVTIDTPHVEALLRGRVVMVTGAGGSIGSELCR CHHHHCCCHHHHHHHHHCCHHHHCCCCCEEECCHHHHHHHCCCEEEEECCCCCHHHHHHH QILRFAPAQLVAFDLSEYAMYRLTEELRERFPDQPVVPIIGDAKDSLLLDQVMSRHVPHI HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHCCCCHH VFHAAAYKHVPLMEEHNAWQALRNNVLGTYRVARAAIRHDVRHFVLISTDKAVNPTNVMG HHHHHHHHCCCCHHHCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHH ASKRLAEMACQALQQTSGRTQFETVRFGNVLGSAGSVIPKFQQQIAKGGPVTVTHPQITR HHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEECHHHHH FFMTIPEASQLVLQASSMGHGGEIFILDMGEPVKIVDLACDLIRLYGFSEDQIQIEFTGL HHHCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEECC RPGEKLYEELLADDETTTRTPHPKLRIARAREVPDNFLDELLPWLMQHRVLPDDEVRRDL CCHHHHHHHHHCCCCCCCCCCCCCEEEHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHH RRWVPEYQTAVPPTLQSVPSVRVVSNG HHHCCHHHHCCCCHHHHCCCEEEECCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NAD+ [C]
Metal ions: NA
Kcat value (1/min): 57600 [C]
Specific activity: 233.3
Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]
Substrates: UDPglucose [C]
Specific reaction: UDPglucose <==> UDPglucoseal [C]
General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 8969506; 9384377 [H]