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The map label for this gene is citH [H]

Identifier: 78065281

GI number: 78065281

Start: 699159

End: 700436

Strand: Direct

Name: citH [H]

Synonym: Bcep18194_A3805

Alternate gene names: 78065281

Gene position: 699159-700436 (Clockwise)

Preceding gene: 78065280

Following gene: 78065288

Centisome position: 18.93

GC content: 67.84

Gene sequence:

>1278_bases
ATGCATGCCGTCACGCAGTCCCGTTCCATTGCCTCGTCGCCGCGCGTGTGGCGCGCGGTGGTCGCCGCATCGATCGGCAA
CGCGCTCGAGTGGTTCGATCTCGTCGTCTACGGCTTCTTCGCGGTGACGATTTCCAAGCTGTTCTTCCCGGCCGGCAACG
ACACCGTGTCGCTGCTGCTCACGCTCGGCACGTTCGGCGTGTCGTTCTTCATGCGTCCGCTCGGCGCGATCGTGCTCGGT
GCATATGCCGACCGCGAGGGCCGCAAGGCCGCGCTCACGCTGTCGATCCTGCTGATGATGGGCGGCACGCTGATCATCGC
GGTGCTGCCGACCTACGAGACGATCGGCGTCGCGGCGCCGGTGATCCTCGTCGCCGCACGGCTGATGCAGGGCTTCTCGG
CCGGCGGCGAGTTCGGCAGCGCGACCGCGTTCCTCGCCGAGCACGTGCCGGGCCGGCGCGGCTTCTTCGCGAGCTGGCAG
GTCGCGAGCCAGGGGCTCACGACGCTGCTCGCCGCCGGCTTCGGCACCGTGCTGAACGCGCAGCTCACGGCCGCGCAGAT
GGCGTCATGGGGCTGGCGCGTGCCGTTCTTCTTCGGCCTGCTGCTCGGGCCGGTCGCGTACTACATCCGCACGAAGGTCG
ACGAGACCCCCGAATTCCTCGCGGCGGAAGGCACCGCGAACCCGCTGCGCGACACGTTCGCGTCGCACAAGGCGCGCCTC
GTTGCTGCAATGGGCGTGGTCGTGCTCGGCACCGTCGCGACCTATCTCGTGCTGTTCATGCCGACCTACGGCGTGAAGCA
GCTTGGCCTCGCGCCGTCGGCTGCGTTCGCGGCGATCCTCGTCGTCGGCGTGATCCAGATGGCGTTCGCGCCGCTCGTCG
GCCACTGGTCGGACACTTACGGCCGCGTGCGCGTGATGATCGCGCCGGCCATCGGCATCCTCGTGCTGATCTATCCGGCA
TTCGCGTACCTGGTCGCCCATCCGGGCTTCGGCACGCTGATCGCGCTGCAGGTGCTGCTCGCGTTCCTGATGACGGGCTA
TTTCGCGGCGCTGCCGGGTTTGCTGTCCGAAGTGTTTCCGGTGCAGACGCGCACGACCGGGATGTCGCTCGCCTATAACG
TCGCGGTGACGATCTTCGGCGGCTTCGGGCCGTTCATCATCGCGTGGCTGATCAAGGCGACCGGGATGAAGACAGCGCCG
AGCTTCTACCTGATGTTCGCGGCCGTACTGAGCCTCGTGGCGCTGGTCGTGCTGCGCAAGCGGTTCGGGTTCCGGTAA

Upstream 100 bases:

>100_bases
CGCGCCTATAATCGCCCGAGCGCCGTACCCGGCGCCGGGCAGGCCGACGGCGCGCGAGCGGCCGCCTCGAACAGAACGTA
CAACGACAAGGAGATCGCCG

Downstream 100 bases:

>100_bases
CCGGGCGGTGCGGCGCGGGGCGCGTCAGTTGCCGGCGCGCTCGCATACGGGCCGTGCCGGCATCAGTTGCGGTTTGGCTT
CGATGGTCCGCACCGGGCGC

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MHAVTQSRSIASSPRVWRAVVAASIGNALEWFDLVVYGFFAVTISKLFFPAGNDTVSLLLTLGTFGVSFFMRPLGAIVLG
AYADREGRKAALTLSILLMMGGTLIIAVLPTYETIGVAAPVILVAARLMQGFSAGGEFGSATAFLAEHVPGRRGFFASWQ
VASQGLTTLLAAGFGTVLNAQLTAAQMASWGWRVPFFFGLLLGPVAYYIRTKVDETPEFLAAEGTANPLRDTFASHKARL
VAAMGVVVLGTVATYLVLFMPTYGVKQLGLAPSAAFAAILVVGVIQMAFAPLVGHWSDTYGRVRVMIAPAIGILVLIYPA
FAYLVAHPGFGTLIALQVLLAFLMTGYFAALPGLLSEVFPVQTRTTGMSLAYNVAVTIFGGFGPFIIAWLIKATGMKTAP
SFYLMFAAVLSLVALVVLRKRFGFR

Sequences:

>Translated_425_residues
MHAVTQSRSIASSPRVWRAVVAASIGNALEWFDLVVYGFFAVTISKLFFPAGNDTVSLLLTLGTFGVSFFMRPLGAIVLG
AYADREGRKAALTLSILLMMGGTLIIAVLPTYETIGVAAPVILVAARLMQGFSAGGEFGSATAFLAEHVPGRRGFFASWQ
VASQGLTTLLAAGFGTVLNAQLTAAQMASWGWRVPFFFGLLLGPVAYYIRTKVDETPEFLAAEGTANPLRDTFASHKARL
VAAMGVVVLGTVATYLVLFMPTYGVKQLGLAPSAAFAAILVVGVIQMAFAPLVGHWSDTYGRVRVMIAPAIGILVLIYPA
FAYLVAHPGFGTLIALQVLLAFLMTGYFAALPGLLSEVFPVQTRTTGMSLAYNVAVTIFGGFGPFIIAWLIKATGMKTAP
SFYLMFAAVLSLVALVVLRKRFGFR
>Mature_425_residues
MHAVTQSRSIASSPRVWRAVVAASIGNALEWFDLVVYGFFAVTISKLFFPAGNDTVSLLLTLGTFGVSFFMRPLGAIVLG
AYADREGRKAALTLSILLMMGGTLIIAVLPTYETIGVAAPVILVAARLMQGFSAGGEFGSATAFLAEHVPGRRGFFASWQ
VASQGLTTLLAAGFGTVLNAQLTAAQMASWGWRVPFFFGLLLGPVAYYIRTKVDETPEFLAAEGTANPLRDTFASHKARL
VAAMGVVVLGTVATYLVLFMPTYGVKQLGLAPSAAFAAILVVGVIQMAFAPLVGHWSDTYGRVRVMIAPAIGILVLIYPA
FAYLVAHPGFGTLIALQVLLAFLMTGYFAALPGLLSEVFPVQTRTTGMSLAYNVAVTIFGGFGPFIIAWLIKATGMKTAP
SFYLMFAAVLSLVALVVLRKRFGFR

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=427, Percent_Identity=32.0843091334895, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1788942, Length=434, Percent_Identity=36.6359447004608, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI1788292, Length=421, Percent_Identity=29.9287410926366, Blast_Score=174, Evalue=9e-45,
Organism=Escherichia coli, GI1789941, Length=427, Percent_Identity=34.192037470726, Blast_Score=161, Evalue=7e-41,
Organism=Escherichia coli, GI87082231, Length=459, Percent_Identity=25.2723311546841, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI87082404, Length=382, Percent_Identity=24.3455497382199, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45194; Mature: 45194

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCC
>Mature Secondary Structure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HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]