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The map label for this gene is dctA2 [H]

Identifier: 78065208

GI number: 78065208

Start: 622491

End: 623813

Strand: Direct

Name: dctA2 [H]

Synonym: Bcep18194_A3732

Alternate gene names: 78065208

Gene position: 622491-623813 (Clockwise)

Preceding gene: 161702967

Following gene: 78065209

Centisome position: 16.85

GC content: 65.38

Gene sequence:

>1323_bases
GTGTCGAAATTCCTCAATTCGCTGTTTGGCCGAGTAGTCGTCGCATTGATTCTAGGGATCGCGCTCGGCGCGTTCTTCCC
GCATTTCGCCGAGTCGTTGCGACCGCTCGGCGACGGCTTCCTGAAGCTCATCAAGATGGTGATCGGGCCGATCGTGTTCT
GCGTCGTGGTCAGCGGGATGGCCAACGCGGGCGACCTGAAGAAGGTCGGCCGGGTCGGCCTGAAGGCCGTCATCTACTTC
GAGATCATGACGACGCTCGCGCTCGGCATCGGCCTCATCCTCGCGTGGCTCACGCGGCCGGGCGTCGGGATGAACATCGA
CCTGCGCTCGCTCGACGCGTCGTCGCTCGCGTCGTACGCGAAGAACGCCGAAAGCCTGAAGGACACGGCCGGCTACCTGA
TGAAGATCATCCCCGAGACCGCGATCGACGCGTTCGCGAAGGGCGACATCCTGCAGATCCTCGTGTTCGCGGTGCTGTTC
GGCTCCGCGCTGTCGCTGCTCGGCGACAAGGCGCAGCGCGTCAACTCGCTGATCGAGGAACTGTCGCATGTGTTCTTCCG
GATCATCGGCTTCATCATCAAGCTCGCCCCGCTCGGCGTGCTCGGCGCGATCGCGTTCACGACCGGCAAGTACGGCGTCG
CGTCGCTCAAGCAGCTCGGCTACCTGGTCGCGGTGTTCTACCTGAGCTGCTTCGTGTTCGTCACGGTCGTGCTCGGCACG
GTGATGCGGCTCGCGGGCTTCTCGGTGTTCAAGCTGATCCGCTACCTGCGCGAGGAACTGTCGATCGTGCTCGGCACGGC
GTCGTCGGACGCGGTGCTGCCGCAGGTGATGCGCAAGCTCGAATACATGGGCGTGAAGGATTCGACGGTCGGCCTCGTGA
TCCCGACCGGCTATTCGTTCAACCTCGACGGCTTCTCGATCTACCTGACGCTCGCCGTGCTGTTCATCGCGCAGGCCACC
AACACGCCGCTGTCGACGCATGACCTGATCGTCGTGCTGCTCGTGTCGCTCGTCACGTCGAAGGGCGCGCACGGGATCCC
GGGCTCGGCGATCGTGATCCTCGCGGCGACGCTGTCGGCGATCCCCGCGATTCCGGTGCTCGGCCTCGTGCTGATCCTGC
CGGTCGACTGGTTCGTCGGCATCGCCCGCGCGGTGACCAACCTGATCGGCAACTGCGTCGCGACGGTGGTCGTCGCGGTG
TGGGAGAACGACATCGATCGTGCCCGCGCGAAACGCGTGCTGAACCGCGAGCTGCGCTACGTGCCGGCCGAAGAGGAAGA
CCGCGCGGCGCCGGTCGCCGGCGACCAGGCTCACGCGCTCTGA

Upstream 100 bases:

>100_bases
CCTTGACTCCACTATATCGGCTTCCTACTATCCACCATAACATCACTGGTCTTACCAGTATGAACAGACGAAACTGCAAC
CGTTGGATCGGGAGAGCGCC

Downstream 100 bases:

>100_bases
GCGCACCCCCCGCGCGGGGCGGCTTCCCCTGGTCGTCCCGCGCAACCGAATTCATGTGGCCGGCGCCTCGAAGCGCCGGC
ACGTCATCGAACCTCAGGAG

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MSKFLNSLFGRVVVALILGIALGAFFPHFAESLRPLGDGFLKLIKMVIGPIVFCVVVSGMANAGDLKKVGRVGLKAVIYF
EIMTTLALGIGLILAWLTRPGVGMNIDLRSLDASSLASYAKNAESLKDTAGYLMKIIPETAIDAFAKGDILQILVFAVLF
GSALSLLGDKAQRVNSLIEELSHVFFRIIGFIIKLAPLGVLGAIAFTTGKYGVASLKQLGYLVAVFYLSCFVFVTVVLGT
VMRLAGFSVFKLIRYLREELSIVLGTASSDAVLPQVMRKLEYMGVKDSTVGLVIPTGYSFNLDGFSIYLTLAVLFIAQAT
NTPLSTHDLIVVLLVSLVTSKGAHGIPGSAIVILAATLSAIPAIPVLGLVLILPVDWFVGIARAVTNLIGNCVATVVVAV
WENDIDRARAKRVLNRELRYVPAEEEDRAAPVAGDQAHAL

Sequences:

>Translated_440_residues
MSKFLNSLFGRVVVALILGIALGAFFPHFAESLRPLGDGFLKLIKMVIGPIVFCVVVSGMANAGDLKKVGRVGLKAVIYF
EIMTTLALGIGLILAWLTRPGVGMNIDLRSLDASSLASYAKNAESLKDTAGYLMKIIPETAIDAFAKGDILQILVFAVLF
GSALSLLGDKAQRVNSLIEELSHVFFRIIGFIIKLAPLGVLGAIAFTTGKYGVASLKQLGYLVAVFYLSCFVFVTVVLGT
VMRLAGFSVFKLIRYLREELSIVLGTASSDAVLPQVMRKLEYMGVKDSTVGLVIPTGYSFNLDGFSIYLTLAVLFIAQAT
NTPLSTHDLIVVLLVSLVTSKGAHGIPGSAIVILAATLSAIPAIPVLGLVLILPVDWFVGIARAVTNLIGNCVATVVVAV
WENDIDRARAKRVLNRELRYVPAEEEDRAAPVAGDQAHAL
>Mature_439_residues
SKFLNSLFGRVVVALILGIALGAFFPHFAESLRPLGDGFLKLIKMVIGPIVFCVVVSGMANAGDLKKVGRVGLKAVIYFE
IMTTLALGIGLILAWLTRPGVGMNIDLRSLDASSLASYAKNAESLKDTAGYLMKIIPETAIDAFAKGDILQILVFAVLFG
SALSLLGDKAQRVNSLIEELSHVFFRIIGFIIKLAPLGVLGAIAFTTGKYGVASLKQLGYLVAVFYLSCFVFVTVVLGTV
MRLAGFSVFKLIRYLREELSIVLGTASSDAVLPQVMRKLEYMGVKDSTVGLVIPTGYSFNLDGFSIYLTLAVLFIAQATN
TPLSTHDLIVVLLVSLVTSKGAHGIPGSAIVILAATLSAIPAIPVLGLVLILPVDWFVGIARAVTNLIGNCVATVVVAVW
ENDIDRARAKRVLNRELRYVPAEEEDRAAPVAGDQAHAL

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=456, Percent_Identity=25.219298245614, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI21314632, Length=450, Percent_Identity=25.5555555555556, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI4827012, Length=300, Percent_Identity=28.3333333333333, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI5032093, Length=426, Percent_Identity=26.5258215962441, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI40254478, Length=485, Percent_Identity=24.3298969072165, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI223468564, Length=301, Percent_Identity=28.2392026578073, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI223468566, Length=301, Percent_Identity=28.2392026578073, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI66773030, Length=393, Percent_Identity=25.1908396946565, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI194239697, Length=295, Percent_Identity=27.4576271186441, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI301601644, Length=214, Percent_Identity=26.6355140186916, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI262359914, Length=390, Percent_Identity=24.3589743589744, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1789947, Length=416, Percent_Identity=52.4038461538462, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI1790514, Length=417, Percent_Identity=38.3693045563549, Blast_Score=284, Evalue=6e-78,
Organism=Escherichia coli, GI1788024, Length=401, Percent_Identity=22.9426433915212, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789473, Length=333, Percent_Identity=24.3243243243243, Blast_Score=68, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17537407, Length=393, Percent_Identity=26.7175572519084, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71996953, Length=455, Percent_Identity=25.7142857142857, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI193206654, Length=471, Percent_Identity=25.2653927813163, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI193206505, Length=471, Percent_Identity=25.4777070063694, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17541374, Length=406, Percent_Identity=25.615763546798, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71983106, Length=452, Percent_Identity=22.787610619469, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71983099, Length=453, Percent_Identity=22.7373068432671, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24583025, Length=419, Percent_Identity=25.0596658711217, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI17137668, Length=419, Percent_Identity=25.0596658711217, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24583023, Length=419, Percent_Identity=25.0596658711217, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI281360481, Length=451, Percent_Identity=26.8292682926829, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI281360483, Length=451, Percent_Identity=26.8292682926829, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17137666, Length=451, Percent_Identity=26.8292682926829, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 46968; Mature: 46836

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKFLNSLFGRVVVALILGIALGAFFPHFAESLRPLGDGFLKLIKMVIGPIVFCVVVSGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ANAGDLKKVGRVGLKAVIYFEIMTTLALGIGLILAWLTRPGVGMNIDLRSLDASSLASYA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHH
KNAESLKDTAGYLMKIIPETAIDAFAKGDILQILVFAVLFGSALSLLGDKAQRVNSLIEE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSHVFFRIIGFIIKLAPLGVLGAIAFTTGKYGVASLKQLGYLVAVFYLSCFVFVTVVLGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMRLAGFSVFKLIRYLREELSIVLGTASSDAVLPQVMRKLEYMGVKDSTVGLVIPTGYSF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCEE
NLDGFSIYLTLAVLFIAQATNTPLSTHDLIVVLLVSLVTSKGAHGIPGSAIVILAATLSA
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
IPAIPVLGLVLILPVDWFVGIARAVTNLIGNCVATVVVAVWENDIDRARAKRVLNRELRY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHC
VPAEEEDRAAPVAGDQAHAL
CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]