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The map label for this gene is exoA [H]

Identifier: 78049571

GI number: 78049571

Start: 4613083

End: 4613883

Strand: Reverse

Name: exoA [H]

Synonym: XCV4015

Alternate gene names: 78049571

Gene position: 4613883-4613083 (Counterclockwise)

Preceding gene: 78049581

Following gene: 78049570

Centisome position: 89.1

GC content: 65.29

Gene sequence:

>801_bases
ATGCGCATCATCAGTTTCAACGCCAACGGCCTGCGCTCGGCCGCCAGCAAGGGTTTCTTCGCGTGGTTCGCCGCCCAGGA
CGCCGACGTGCTGTGCGTGCAGGAAACCAAGGCCCAGGAGCATCAGCTGGCCGGGCCAGACTTCCTGCCCACCGGCTACA
AGGCCTGGTTTCGCGACGCCAGCACCAAGAAGGGCTATAGCGGCATGGCGATCTACAGCCGGCACGAGCCCGACGAGGTG
CGCACCGCGCTGGGCTGGCCGGAGTTCGACGAAGAGGGGCGCTATATCGAAGCGCGCTTCGGCAACCTCAGCGTGGTGTC
CTTCTACATCCCCTCCGGTTCCTCGGGCGAGTTGCGCCAGGACTACAAGTTCCAGGTGATGCAATGGCTACGGCCGATCC
TGGAGCAGTGGCTGGCCAGTGGCCGCCAGTACGTGCTGTGCGGCGACTGGAACATCGTGCGCACGGCGCTGGACATCAAG
AACTGGAAATCCAACCAGAAGAACTCTGGCTGCCTGCCGCCCGAGCGCGATTGGCTCAACGGCCTGTGCGCCGACGCGCC
AGAAGACGCCAACCCCGCCGAAGGCCGCGGCTGGGTCGACAGCTACCGCGTACTGCATCCGCAGGGCCAGGACTACACCT
GGTGGAGCAACCGTGGCGCGGCGCGCGCCAACAATGTCGGTTGGCGCATCGACTACCAACTGGTCACCCCCGGCCTGCGC
GACACCTTGAAGGCCTGCTCGATCTACCGCGAAGAGCGCTTCTCCGACCACGCGCCGTACATCGTGGATTACGCGCAGTG
A

Upstream 100 bases:

>100_bases
GGGGAATCGGGAATGGTAAACGCATCATGCGCCGGCAGCCTTCCGCATGCGCCTGGGGTAGGCTTTACGATTCCCCATTC
CCGTTTCCCATTCCCGGCCT

Downstream 100 bases:

>100_bases
GGCAGGCCAAGGCTATGGTTTCGGTCATAGCGCGCCGGCATGTTGCAGAACGGGGCCAAGACTTCGCCGGCAACATCGCC
ATCCGGCGGCAGCTCGGCCA

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MRIISFNANGLRSAASKGFFAWFAAQDADVLCVQETKAQEHQLAGPDFLPTGYKAWFRDASTKKGYSGMAIYSRHEPDEV
RTALGWPEFDEEGRYIEARFGNLSVVSFYIPSGSSGELRQDYKFQVMQWLRPILEQWLASGRQYVLCGDWNIVRTALDIK
NWKSNQKNSGCLPPERDWLNGLCADAPEDANPAEGRGWVDSYRVLHPQGQDYTWWSNRGAARANNVGWRIDYQLVTPGLR
DTLKACSIYREERFSDHAPYIVDYAQ

Sequences:

>Translated_266_residues
MRIISFNANGLRSAASKGFFAWFAAQDADVLCVQETKAQEHQLAGPDFLPTGYKAWFRDASTKKGYSGMAIYSRHEPDEV
RTALGWPEFDEEGRYIEARFGNLSVVSFYIPSGSSGELRQDYKFQVMQWLRPILEQWLASGRQYVLCGDWNIVRTALDIK
NWKSNQKNSGCLPPERDWLNGLCADAPEDANPAEGRGWVDSYRVLHPQGQDYTWWSNRGAARANNVGWRIDYQLVTPGLR
DTLKACSIYREERFSDHAPYIVDYAQ
>Mature_266_residues
MRIISFNANGLRSAASKGFFAWFAAQDADVLCVQETKAQEHQLAGPDFLPTGYKAWFRDASTKKGYSGMAIYSRHEPDEV
RTALGWPEFDEEGRYIEARFGNLSVVSFYIPSGSSGELRQDYKFQVMQWLRPILEQWLASGRQYVLCGDWNIVRTALDIK
NWKSNQKNSGCLPPERDWLNGLCADAPEDANPAEGRGWVDSYRVLHPQGQDYTWWSNRGAARANNVGWRIDYQLVTPGLR
DTLKACSIYREERFSDHAPYIVDYAQ

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=264, Percent_Identity=35.2272727272727, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI18375503, Length=264, Percent_Identity=35.2272727272727, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI18375501, Length=264, Percent_Identity=35.2272727272727, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI18375507, Length=310, Percent_Identity=26.4516129032258, Blast_Score=89, Evalue=5e-18,
Organism=Escherichia coli, GI1788046, Length=278, Percent_Identity=30.2158273381295, Blast_Score=102, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71989536, Length=262, Percent_Identity=25.9541984732824, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI221330655, Length=266, Percent_Identity=30.8270676691729, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17136678, Length=266, Percent_Identity=30.8270676691729, Blast_Score=122, Evalue=3e-28,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30396; Mature: 30396

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIISFNANGLRSAASKGFFAWFAAQDADVLCVQETKAQEHQLAGPDFLPTGYKAWFRDA
CEEEEECCCCHHHHHCCCEEEEEECCCCCEEEEECCCCHHHHCCCCCCCCCCHHHHHCCC
STKKGYSGMAIYSRHEPDEVRTALGWPEFDEEGRYIEARFGNLSVVSFYIPSGSSGELRQ
CCCCCCCCEEEEECCCHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEEECCCCCCCCCHH
DYKFQVMQWLRPILEQWLASGRQYVLCGDWNIVRTALDIKNWKSNQKNSGCLPPERDWLN
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEHHHHHHHHCCCCCCCCCCCCCCHHHHC
GLCADAPEDANPAEGRGWVDSYRVLHPQGQDYTWWSNRGAARANNVGWRIDYQLVTPGLR
CCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEECCCCCCCCCCCCEEEEEEEECCCHH
DTLKACSIYREERFSDHAPYIVDYAQ
HHHHHHHHHHHHHCCCCCCEEEEECC
>Mature Secondary Structure
MRIISFNANGLRSAASKGFFAWFAAQDADVLCVQETKAQEHQLAGPDFLPTGYKAWFRDA
CEEEEECCCCHHHHHCCCEEEEEECCCCCEEEEECCCCHHHHCCCCCCCCCCHHHHHCCC
STKKGYSGMAIYSRHEPDEVRTALGWPEFDEEGRYIEARFGNLSVVSFYIPSGSSGELRQ
CCCCCCCCEEEEECCCHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEEECCCCCCCCCHH
DYKFQVMQWLRPILEQWLASGRQYVLCGDWNIVRTALDIKNWKSNQKNSGCLPPERDWLN
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEHHHHHHHHCCCCCCCCCCCCCCHHHHC
GLCADAPEDANPAEGRGWVDSYRVLHPQGQDYTWWSNRGAARANNVGWRIDYQLVTPGLR
CCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEECCCCCCCCCCCCEEEEEEEECCCHH
DTLKACSIYREERFSDHAPYIVDYAQ
HHHHHHHHHHHHHCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]