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The map label for this gene is gumJ [H]

Identifier: 78048335

GI number: 78048335

Start: 3156992

End: 3158503

Strand: Reverse

Name: gumJ [H]

Synonym: XCV2779

Alternate gene names: 78048335

Gene position: 3158503-3156992 (Counterclockwise)

Preceding gene: 78048336

Following gene: 78048334

Centisome position: 60.99

GC content: 64.55

Gene sequence:

>1512_bases
GTGACCGCAGAGACACCTACGATGACATCTTCCACACCGCCGCCACCGCCACAGCGCAGCCTGGGGTCGCGCGCTGCCGG
CGGCGCAGCGGTGACCATGCTGGGGCAGTCGGCCAAGATGGTGGTGCAGTTCGGCGGCATCATCCTGTTGGCGCGCTTGC
TGACGCCCTACGACTACGGTCTGATGGCGATGGTGACCGCCATCGTCGGCGCCGCCGAGATCCTGCGCGATTTCGGTCTG
TCGGCCGCGGCTGTGCAAGCCAAGCAAGTGAGTCGCGAGCAGCGCGACAACCTGTTCTGGATCAACAGCGGCATTGGCCT
GCTGCTGTCGCTGGTGGTGTTCGCCTCGGCGCATCTGATTGCGAACTTCTACAAGGAGCCGGCCCTGGTGACGATTTCCC
AGGCGCTGGCGGTCACCTTCCTGATCAACGGCATGACCACGCAGTACCGCGCGCATCTGAGCCGCGGGCTGCGCTTCGGG
CAGGTGGCGCTGAGCGATGTGGGCTCGCAGGTGCTCGGTCTGGGCGCAGCGGTTGCTGCTGCGCTGCTGGGCTGGGGCTA
CTGGGCGCTGGTGGTACAGCAGGTGGTGCAGGCCGGCGTCAATTTCATCATCGCCGCCAGCTGCGCGCGCTGGTTGCCGG
GTGGCTACCAGCGTTCTGCACCGATGCGCGATTTCATGAGTTTCGGCTGGAACCTGATGGCGGCGCAGTTGCTGGGGTAT
GCCAGTCGCAACGTGGGCCAGGTCATCATCGGTTGGCGGACCGGACCGGACGCGCTGGGCTTGTACAACCGCGCATTCCA
GTTGTTGATGATGCCGTTGAACCAGATCAATGCGCCTGCCACCAGCGTGGCGCTGCCGGTGTTGTCGCAGCTGCAGGACG
ACCGCGAGCGCTTCAGTGCCTTCCTGTTGCGCGGGCAGACCGTGATGGTGCACCTGATCTTTGCGCTGTTTGCGTTCGCC
TGCGCGCTGGCGATGCCGCTGATTGTGCTGGTGCTGGGCGAGCAGTGGCGTGAGGCGGTGCCGCTGTTCCAGGTGCTGAC
ACTGGGCGGTATCTTCCAGACGGCCGCTTATGCAACGTATTGGGTGTTCCTTGCGCGGGGACTGATGCGCGCGCAGCTGG
TGTATTCGCTGGTCAGTCGCGTCCTGCTGATTGCCTGCATCTTCATCGGCTCGCGCTGGGGCGCAATGGGGGTGGCGATC
GGCTATTCGTTCGGTCTGTTGTTGACCTGGCCGCTGTCGCTGCTGTGGATCGGCAAGATTTCAGACGCGCCGGTCGGAGC
CCTGTTCACCAATGCACTGCGCGCCATGGTCGCCTACGGCGTGGCAGGTGGCTGTGCGTATTACGCCTCCATGACGGTTG
GCGGCCCGCTGTGGGAGCAACTGGCTGTCGGCGCAGTGACGATGCTTGCCGTTTGCCTGCTTGCCTTGGCGATCTGGCCG
GCATTCCGTCGCGATGTGATTGCCATCATCAACATCCGCAAGCTGCTGACGCAGGCCAAGGCACGTCGATGA

Upstream 100 bases:

>100_bases
GCCCGGCACCGGATCTGTCGCAGCGCGACTGGCCGCGGATCGGGCAGTTGCATTACCGCACCTATCTGGAAGCGTTGGGC
AAGGATGGAGACGCCGCGCT

Downstream 100 bases:

>100_bases
GTCGACATTGTCTTCGCCTGCAGTCGGTCATGCTTTCTCTCACTCATTCATAGGACACGGCCCATGAGCGACACACCCGC
CGCCGCTTCCGGCATCCGCC

Product: xanthan biosynthesis oligosaccharidyl-lipid flippase GumJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MTAETPTMTSSTPPPPPQRSLGSRAAGGAAVTMLGQSAKMVVQFGGIILLARLLTPYDYGLMAMVTAIVGAAEILRDFGL
SAAAVQAKQVSREQRDNLFWINSGIGLLLSLVVFASAHLIANFYKEPALVTISQALAVTFLINGMTTQYRAHLSRGLRFG
QVALSDVGSQVLGLGAAVAAALLGWGYWALVVQQVVQAGVNFIIAASCARWLPGGYQRSAPMRDFMSFGWNLMAAQLLGY
ASRNVGQVIIGWRTGPDALGLYNRAFQLLMMPLNQINAPATSVALPVLSQLQDDRERFSAFLLRGQTVMVHLIFALFAFA
CALAMPLIVLVLGEQWREAVPLFQVLTLGGIFQTAAYATYWVFLARGLMRAQLVYSLVSRVLLIACIFIGSRWGAMGVAI
GYSFGLLLTWPLSLLWIGKISDAPVGALFTNALRAMVAYGVAGGCAYYASMTVGGPLWEQLAVGAVTMLAVCLLALAIWP
AFRRDVIAIINIRKLLTQAKARR

Sequences:

>Translated_503_residues
MTAETPTMTSSTPPPPPQRSLGSRAAGGAAVTMLGQSAKMVVQFGGIILLARLLTPYDYGLMAMVTAIVGAAEILRDFGL
SAAAVQAKQVSREQRDNLFWINSGIGLLLSLVVFASAHLIANFYKEPALVTISQALAVTFLINGMTTQYRAHLSRGLRFG
QVALSDVGSQVLGLGAAVAAALLGWGYWALVVQQVVQAGVNFIIAASCARWLPGGYQRSAPMRDFMSFGWNLMAAQLLGY
ASRNVGQVIIGWRTGPDALGLYNRAFQLLMMPLNQINAPATSVALPVLSQLQDDRERFSAFLLRGQTVMVHLIFALFAFA
CALAMPLIVLVLGEQWREAVPLFQVLTLGGIFQTAAYATYWVFLARGLMRAQLVYSLVSRVLLIACIFIGSRWGAMGVAI
GYSFGLLLTWPLSLLWIGKISDAPVGALFTNALRAMVAYGVAGGCAYYASMTVGGPLWEQLAVGAVTMLAVCLLALAIWP
AFRRDVIAIINIRKLLTQAKARR
>Mature_502_residues
TAETPTMTSSTPPPPPQRSLGSRAAGGAAVTMLGQSAKMVVQFGGIILLARLLTPYDYGLMAMVTAIVGAAEILRDFGLS
AAAVQAKQVSREQRDNLFWINSGIGLLLSLVVFASAHLIANFYKEPALVTISQALAVTFLINGMTTQYRAHLSRGLRFGQ
VALSDVGSQVLGLGAAVAAALLGWGYWALVVQQVVQAGVNFIIAASCARWLPGGYQRSAPMRDFMSFGWNLMAAQLLGYA
SRNVGQVIIGWRTGPDALGLYNRAFQLLMMPLNQINAPATSVALPVLSQLQDDRERFSAFLLRGQTVMVHLIFALFAFAC
ALAMPLIVLVLGEQWREAVPLFQVLTLGGIFQTAAYATYWVFLARGLMRAQLVYSLVSRVLLIACIFIGSRWGAMGVAIG
YSFGLLLTWPLSLLWIGKISDAPVGALFTNALRAMVAYGVAGGCAYYASMTVGGPLWEQLAVGAVTMLAVCLLALAIWPA
FRRDVIAIINIRKLLTQAKARR

Specific function: Might be involved in the translocation of teichuronic acid repeating units from the inner to the outer surface of the membrane [H]

COG id: COG2244

COG function: function code R; Membrane protein involved in the export of O-antigen and teichoic acid

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788359, Length=361, Percent_Identity=23.5457063711911, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002797 [H]

Pfam domain/function: PF01943 Polysacc_synt [H]

EC number: NA

Molecular weight: Translated: 54059; Mature: 53928

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TAETPTMTSSTPPPPPQRSLGSRAAGGAAVTMLGQSAKMVVQFGGIILLARLLTPYDYG
CCCCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCH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HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10048024; 9384377 [H]