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The map label for this gene is insK [C]

Identifier: 78048328

GI number: 78048328

Start: 3151891

End: 3152724

Strand: Reverse

Name: insK [C]

Synonym: XCV2772

Alternate gene names: 78048328

Gene position: 3152724-3151891 (Counterclockwise)

Preceding gene: 78048329

Following gene: 78048327

Centisome position: 60.88

GC content: 62.95

Gene sequence:

>834_bases
ATGCTCGAACACACCCCGTTGAGCGAGCGCCGGGCTTGCCGCCTTGCGGGGCTGTCCCGCGATGCTTTCCGCCACGCACC
CGTGCCGACGCCAGCCACGCAGGCGCTGTCGGCGCGGCTGGTCGAGCTGGCCCAGACCCACCGCCGCTTCGGCTATCGCC
GACTGCATGATTTGTTGCGCCCGGAGTTTCCATCCGTGAATCACAAAAAGATTTATCGCCTGTACGAGGAAGCCGAGCTG
AAGGTGCGCAAACGGCGCAAGGCCAAGCGTCCGGTGGGTGAGCGGCAGAAACTGCTGGCGTCCTCCATGCCGAACGACAC
CTGGAGCATGGACTTCGTGTTCGATGCGCTGGCCAACGCACGCCGCATCAAGTGCCTGACCGTGGTCGACGACTTCACTC
GCGAGAGCGTGGACATTGCCGTGGATCACGGCATCAGCGGGGCTTACGTAGTGCGCCTGCTCGATCAGGCGGCGTGTTTC
CGTGGCTATCCACGCGCGGTCCGCACCGACAATGGCCCCGAGTTCACCAGTCGCGCCTTCATTGCCTGGACGCAGCAACA
TGGCATCGAGCACATCCTGATCGAGCCGGGTGCCCCCACGCAGAACGCCTACATCGAAAGCTTCAACGGCAAGTTCCGCG
ACGAGTGCCTCAACGAGCACTGGTTCACCTCGTTGGCACAAGCCCGGGACGTGATCGCAGACTGGCGACGCCACTACAAC
CAGATCAGGCCGCACAGCAGTTGTGGTCGCATCCCGCCGGCACAGTTCGCCGCCAACTACCGTACACAACAAGCCAACAA
CGCAGTACCCTTCAACCCCGGGCTTTATCAGTAA

Upstream 100 bases:

>100_bases
AGAAGTTGCTGGCCGAGGCGCACCTGGACATCGAAGCGCTGAAGGTTGGCTTCGGGGTAAAACGCTAGCCCCGCAACGCA
AGCGCGAGGCAATCCGACGA

Downstream 100 bases:

>100_bases
TCACTGGTACGGACGATGGGGGCAGGTCAGAGCGTGTCCAAGGCGCGCTATCTCGGCAGCGACCGCGGCGTTGAGCGCAA
ACGCCCGATGCTGGCTGCCG

Product: IS1477 transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MLEHTPLSERRACRLAGLSRDAFRHAPVPTPATQALSARLVELAQTHRRFGYRRLHDLLRPEFPSVNHKKIYRLYEEAEL
KVRKRRKAKRPVGERQKLLASSMPNDTWSMDFVFDALANARRIKCLTVVDDFTRESVDIAVDHGISGAYVVRLLDQAACF
RGYPRAVRTDNGPEFTSRAFIAWTQQHGIEHILIEPGAPTQNAYIESFNGKFRDECLNEHWFTSLAQARDVIADWRRHYN
QIRPHSSCGRIPPAQFAANYRTQQANNAVPFNPGLYQ

Sequences:

>Translated_277_residues
MLEHTPLSERRACRLAGLSRDAFRHAPVPTPATQALSARLVELAQTHRRFGYRRLHDLLRPEFPSVNHKKIYRLYEEAEL
KVRKRRKAKRPVGERQKLLASSMPNDTWSMDFVFDALANARRIKCLTVVDDFTRESVDIAVDHGISGAYVVRLLDQAACF
RGYPRAVRTDNGPEFTSRAFIAWTQQHGIEHILIEPGAPTQNAYIESFNGKFRDECLNEHWFTSLAQARDVIADWRRHYN
QIRPHSSCGRIPPAQFAANYRTQQANNAVPFNPGLYQ
>Mature_277_residues
MLEHTPLSERRACRLAGLSRDAFRHAPVPTPATQALSARLVELAQTHRRFGYRRLHDLLRPEFPSVNHKKIYRLYEEAEL
KVRKRRKAKRPVGERQKLLASSMPNDTWSMDFVFDALANARRIKCLTVVDDFTRESVDIAVDHGISGAYVVRLLDQAACF
RGYPRAVRTDNGPEFTSRAFIAWTQQHGIEHILIEPGAPTQNAYIESFNGKFRDECLNEHWFTSLAQARDVIADWRRHYN
QIRPHSSCGRIPPAQFAANYRTQQANNAVPFNPGLYQ

Specific function: Involved in the transposition of the insertion sequence is150 [C]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain

Homologues:

Organism=Escherichia coli, GI1789981, Length=269, Percent_Identity=25.6505576208178, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788405, Length=233, Percent_Identity=24.8927038626609, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787263, Length=233, Percent_Identity=24.8927038626609, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786753, Length=233, Percent_Identity=24.8927038626609, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786570, Length=233, Percent_Identity=24.8927038626609, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786490, Length=233, Percent_Identity=24.8927038626609, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YI61_XANEU (P25438)

Other databases:

- EMBL:   M28557
- ProteinModelPortal:   P25438
- SMR:   P25438
- InterPro:   IPR001584
- InterPro:   IPR012337

Pfam domain/function: PF00665 rve; SSF53098 RNaseH_fold

EC number: NA

Molecular weight: Translated: 31851; Mature: 31851

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEHTPLSERRACRLAGLSRDAFRHAPVPTPATQALSARLVELAQTHRRFGYRRLHDLLR
CCCCCCCHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PEFPSVNHKKIYRLYEEAELKVRKRRKAKRPVGERQKLLASSMPNDTWSMDFVFDALANA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCC
RRIKCLTVVDDFTRESVDIAVDHGISGAYVVRLLDQAACFRGYPRAVRTDNGPEFTSRAF
CCEEEEEHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
IAWTQQHGIEHILIEPGAPTQNAYIESFNGKFRDECLNEHWFTSLAQARDVIADWRRHYN
EEEHHHCCCEEEEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QIRPHSSCGRIPPAQFAANYRTQQANNAVPFNPGLYQ
HCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MLEHTPLSERRACRLAGLSRDAFRHAPVPTPATQALSARLVELAQTHRRFGYRRLHDLLR
CCCCCCCHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PEFPSVNHKKIYRLYEEAELKVRKRRKAKRPVGERQKLLASSMPNDTWSMDFVFDALANA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCC
RRIKCLTVVDDFTRESVDIAVDHGISGAYVVRLLDQAACFRGYPRAVRTDNGPEFTSRAF
CCEEEEEHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
IAWTQQHGIEHILIEPGAPTQNAYIESFNGKFRDECLNEHWFTSLAQARDVIADWRRHYN
EEEHHHCCCEEEEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QIRPHSSCGRIPPAQFAANYRTQQANNAVPFNPGLYQ
HCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2152895