| Definition | Xanthomonas campestris pv. vesicatoria str. 85-10 chromosome, complete genome. |
|---|---|
| Accession | NC_007508 |
| Length | 5,178,466 |
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The map label for this gene is dadA [C]
Identifier: 78048303
GI number: 78048303
Start: 3127043
End: 3128320
Strand: Reverse
Name: dadA [C]
Synonym: XCV2747
Alternate gene names: 78048303
Gene position: 3128320-3127043 (Counterclockwise)
Preceding gene: 78048304
Following gene: 78048302
Centisome position: 60.41
GC content: 71.91
Gene sequence:
>1278_bases ATGGAACCCCAGCGGCCCGCACCGGTGCTTGCGTCGGTCACGCCGTCTGGCGCAGCACTGTCTGCTGCGCTGGCAGGAAC TGCTGCTGCAACGCCTGCGTCGGCAGCGGCGCTTGGATTGGGTGCGGTTGCCGAATCCGGGTCCGCCGCGTCTGCCGCGT ACACGCGACGCAGTTACGACCTGATCGTGATCGGCGCCGGCATTGTGGGCGCCGCTTGCGCGGAAGCGGCGGCGGGCGAG GGCCTTCGCGTGGCGATCGTCGAGCCGGGACCGATCGGCGGCGGCGCCACGGCCGCGGCGATGGGGCATTTGGTGGCGAT GGACGACGACCCCGCCGAGCTGGCGTTGTCGGCGTATTCGCTGCGGCTGTGGGAACGCTTCGCCACGCTCAGCGACGCCG AATTCAGCCGCTGCGGCACGCTGTGGGTGGCCCGCGACGCGCGCGAACTGGCGGCGGTGCCAGCCAAGATCGCGCGCCTG GCAACGGCCGGCCTGCATGCCGAAGCGATCGATGCCGCGCAGCTGTATGCGCTGGAGCCAGAGCTGGTGCCCGGGCTGGC TGGCGGCATGCGCGTGCCCGATGAAGCTGTGGTGTATCCGCCGCGGGTCGCGCGGCATTTGGTGGGCGTGGCCTGCACGT CCGGTGCACGCTTGTTTGCCGGCCGCCGCGTGGTGCAGCTGCAGGCGCATGCGGTGCGTCTGGACGATGGGCAGACGCTC GCCGGGCCGGTGCTGGTGGCCAGCGGTGTGGCCTTGCCGCAGTTGCTGCCCGAGCTGGCACTGCGCCCGCGCAAGGGCCA TTTGGTGATCACCGACCGGCACCCGGGGCTGATCCGGCATCAGCTGCTGGAACTGGGCTATGCGGATTCGGCGCACGGGG CCGACGACACCAGCGTGGCCTTCAATGTGCAGCCGCGGCCCACCGGGCAGATTCTGATCGGCTCCTCGCGCCAGTTCGGC GAACACGACCGCGCCTTGTCGATGCCGGTGCTGCAGCAGATGCTGCAGCGCGCCTTTGCGTACCTGCCGGTCCTGCGCGA GCTGCAGGCGATCCGCGTGTGGACCGGCCTGCGTCCGGCCACGCCGGACGGGCGCCCGTATTTGGGGGTGGTGCCCGGCC GACGCGATGTGTGGGTGGCCGCCGGTCACGAGGGGTTGGGGGTCACCACGGCGCTGGGGAGCGCACGGGTGATCGTGGAC AGCCTGCTCGGGCGCACCCCGGCCATCGACCCGGCGCCCTACGCACCGGCGCGTGCCGTGCAAGGAGCGCCCGCATGA
Upstream 100 bases:
>100_bases GTGGCATCGCACCGCGCATCAGTGGGCGGGCGTACATCACCGCGCGCGCGCAACTGGTGATCGACCCGGCGGACCCGTTC GCCTGGGGCATCGTGGCCTG
Downstream 100 bases:
>100_bases GCGCGACGGTGCGTCTGCAGGTGGACGGGCGCATGGTCGAGGTGCCGGCCGGTGCCAGCGTGGCGGCGGCCGTGGCGCAG GCGACGTTGCAGTTTCGCCA
Product: putative D-amino acid oxidase
Products: FADH2; ammonia; Pyruvate [C]
Alternate protein names: NA
Number of amino acids: Translated: 425; Mature: 425
Protein sequence:
>425_residues MEPQRPAPVLASVTPSGAALSAALAGTAAATPASAAALGLGAVAESGSAASAAYTRRSYDLIVIGAGIVGAACAEAAAGE GLRVAIVEPGPIGGGATAAAMGHLVAMDDDPAELALSAYSLRLWERFATLSDAEFSRCGTLWVARDARELAAVPAKIARL ATAGLHAEAIDAAQLYALEPELVPGLAGGMRVPDEAVVYPPRVARHLVGVACTSGARLFAGRRVVQLQAHAVRLDDGQTL AGPVLVASGVALPQLLPELALRPRKGHLVITDRHPGLIRHQLLELGYADSAHGADDTSVAFNVQPRPTGQILIGSSRQFG EHDRALSMPVLQQMLQRAFAYLPVLRELQAIRVWTGLRPATPDGRPYLGVVPGRRDVWVAAGHEGLGVTTALGSARVIVD SLLGRTPAIDPAPYAPARAVQGAPA
Sequences:
>Translated_425_residues MEPQRPAPVLASVTPSGAALSAALAGTAAATPASAAALGLGAVAESGSAASAAYTRRSYDLIVIGAGIVGAACAEAAAGE GLRVAIVEPGPIGGGATAAAMGHLVAMDDDPAELALSAYSLRLWERFATLSDAEFSRCGTLWVARDARELAAVPAKIARL ATAGLHAEAIDAAQLYALEPELVPGLAGGMRVPDEAVVYPPRVARHLVGVACTSGARLFAGRRVVQLQAHAVRLDDGQTL AGPVLVASGVALPQLLPELALRPRKGHLVITDRHPGLIRHQLLELGYADSAHGADDTSVAFNVQPRPTGQILIGSSRQFG EHDRALSMPVLQQMLQRAFAYLPVLRELQAIRVWTGLRPATPDGRPYLGVVPGRRDVWVAAGHEGLGVTTALGSARVIVD SLLGRTPAIDPAPYAPARAVQGAPA >Mature_425_residues MEPQRPAPVLASVTPSGAALSAALAGTAAATPASAAALGLGAVAESGSAASAAYTRRSYDLIVIGAGIVGAACAEAAAGE GLRVAIVEPGPIGGGATAAAMGHLVAMDDDPAELALSAYSLRLWERFATLSDAEFSRCGTLWVARDARELAAVPAKIARL ATAGLHAEAIDAAQLYALEPELVPGLAGGMRVPDEAVVYPPRVARHLVGVACTSGARLFAGRRVVQLQAHAVRLDDGQTL AGPVLVASGVALPQLLPELALRPRKGHLVITDRHPGLIRHQLLELGYADSAHGADDTSVAFNVQPRPTGQILIGSSRQFG EHDRALSMPVLQQMLQRAFAYLPVLRELQAIRVWTGLRPATPDGRPYLGVVPGRRDVWVAAGHEGLGVTTALGSARVIVD SLLGRTPAIDPAPYAPARAVQGAPA
Specific function: Oxidative Deamination Of D-Amino Acids. [C]
COG id: COG0665
COG function: function code E; Glycine/D-amino acid oxidases (deaminating)
Gene ontology:
Cell location: Inner Membrane-Bound [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the DadA oxidoreductase family [H]
Homologues:
Organism=Escherichia coli, GI1787438, Length=296, Percent_Identity=24.6621621621622, Blast_Score=71, Evalue=1e-13,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR006076 - InterPro: IPR012727 [H]
Pfam domain/function: PF01266 DAO [H]
EC number: 1.4.99.1 [C]
Molecular weight: Translated: 43716; Mature: 43716
Theoretical pI: Translated: 7.35; Mature: 7.35
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 1.4 %Met (Translated Protein) 2.1 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 1.4 %Met (Mature Protein) 2.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MEPQRPAPVLASVTPSGAALSAALAGTAAATPASAAALGLGAVAESGSAASAAYTRRSYD CCCCCCCCEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHCCCCE LIVIGAGIVGAACAEAAAGEGLRVAIVEPGPIGGGATAAAMGHLVAMDDDPAELALSAYS EEEEECCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHCEEEEECCCHHHHHHHHHH LRLWERFATLSDAEFSRCGTLWVARDARELAAVPAKIARLATAGLHAEAIDAAQLYALEP HHHHHHHHHCCCCCHHHCCEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHCHHHEEEECC ELVPGLAGGMRVPDEAVVYPPRVARHLVGVACTSGARLFAGRRVVQLQAHAVRLDDGQTL HHCCCCCCCCCCCCCCEECCHHHHHHHHHHHCCCCCEECCCCEEEEEEEEEEEECCCCCC AGPVLVASGVALPQLLPELALRPRKGHLVITDRHPGLIRHQLLELGYADSAHGADDTSVA CCHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCEEE FNVQPRPTGQILIGSSRQFGEHDRALSMPVLQQMLQRAFAYLPVLRELQAIRVWTGLRPA EEECCCCCCEEEECCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC TPDGRPYLGVVPGRRDVWVAAGHEGLGVTTALGSARVIVDSLLGRTPAIDPAPYAPARAV CCCCCEEEEECCCCCEEEEEECCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCHHCC QGAPA CCCCC >Mature Secondary Structure MEPQRPAPVLASVTPSGAALSAALAGTAAATPASAAALGLGAVAESGSAASAAYTRRSYD CCCCCCCCEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHCCCCE LIVIGAGIVGAACAEAAAGEGLRVAIVEPGPIGGGATAAAMGHLVAMDDDPAELALSAYS EEEEECCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHCEEEEECCCHHHHHHHHHH LRLWERFATLSDAEFSRCGTLWVARDARELAAVPAKIARLATAGLHAEAIDAAQLYALEP HHHHHHHHHCCCCCHHHCCEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHCHHHEEEECC ELVPGLAGGMRVPDEAVVYPPRVARHLVGVACTSGARLFAGRRVVQLQAHAVRLDDGQTL HHCCCCCCCCCCCCCCEECCHHHHHHHHHHHCCCCCEECCCCEEEEEEEEEEEECCCCCC AGPVLVASGVALPQLLPELALRPRKGHLVITDRHPGLIRHQLLELGYADSAHGADDTSVA CCHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCEEE FNVQPRPTGQILIGSSRQFGEHDRALSMPVLQQMLQRAFAYLPVLRELQAIRVWTGLRPA EEECCCCCCEEEECCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC TPDGRPYLGVVPGRRDVWVAAGHEGLGVTTALGSARVIVDSLLGRTPAIDPAPYAPARAV CCCCCEEEEECCCCCEEEEEECCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCHHCC QGAPA CCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: Iron [C]
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 11 {D-methionine}} 6 {D-phenylalanine}} 12 {D-alpha-aminobutyrate}} 0.15 {cytochrome} 19 {D-serine}} 29 {D-alanine}} 6.4 {D-alanine}} 5.3 {D
Substrates: D-Alanine; FAD; H2O [C]
Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]
General reaction: Oxidative deamination; Redox reaction [C]
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 8682785; 10984043; 8097014 [H]