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The map label for this gene is pleD [H]

Identifier: 78047102

GI number: 78047102

Start: 1745757

End: 1747550

Strand: Reverse

Name: pleD [H]

Synonym: XCV1546

Alternate gene names: 78047102

Gene position: 1747550-1745757 (Counterclockwise)

Preceding gene: 78047105

Following gene: 78047100

Centisome position: 33.75

GC content: 66.16

Gene sequence:

>1794_bases
ATGTGCCGTCTGCCAGCTGACTCGGCAAGCGAAGTGGAGCGTAGGCACGCCGCTTGCATCGGCCTCGACCAGACAATCGT
GCCGGGGAACGCGGTGTTGACCAAACGAACCAAACGTCGCGCTGCCGGGGCCTCGCTGGTGGGCGGGCTGATCTTTGTCC
TGATCGGCGTGGGCGGATATCTCACGACGCAGCGTTCGCTGTCGGACGCCGGCTGGGTGACGCACACGCAGGAAGTGATT
GCCAGCATCGACGAAATCCAGGCGGGCATGCTGTCGGCGGAATCGTCGGCGCGCGGCTACGTCCTGACCGACAACGAGGC
CTTTCTGGGCGTGTATGCCGATGCGATTGGCCGCCTGCCCGAGCGCATCGTGCGGCTGGATGCCCTGGTGCAGGACAACC
CGACGCAGCGGCGCAATGTGGTCGTCCTGAGCCGTTTGGTGGACGCGCGCCTGGCGCAGATCGACGAGTTGTTGCATTGC
TATCGCAAGCAAGGCCTGGATGGCGCGCGTGCGGCCATCGCGCGCGGGGTGTTCCAGACCGCTTCATCGCTGCGCCGCCA
GGTGCAGGCCATGACGGAGCTGGAGCGGCAGCAACTGGTCAGGCGCAACGCGGCCACCACGCGCAGCGCACAGTGGGCGC
TGTGGGTGACGGTGATCGGCATTGTCAGTGGCTTGCTGGTGATGCTGCTGGCCTACCGCTTGTTGTCGCGCGAGCTGGAA
CGGCGTACGCGTGCCGAAGACGACGCCAGCCGGACCAGCGAGCGCCTGATCGAGTCGTTTGCCGTACTCGAACGCACCTC
CGCTGGTCTGGAGGCACTGGCACGCTATTCCGGGCTGCTGCAGAACTGCCGCGACGCCGAAGAAGCGCTGGAAATCACTG
CACGCACGATTGCACCGCTGATTCCCGGTGCCGCCGGCGCGGTCTACCTGCTGCGCGCCTCGCGCGACCGCGCCGAACAG
GTGGTTGCCTGGGGCGAGATGGCCGACGACGCCAGTACCAGCATCGCACCGGACAACTGCTGGGCGCTGCGCCGCGACCG
CACCCATGTGGTGATGGACGCCAGCGCCGGGATGCTATGCCAGCATGCCGGCGTCAAGCTCCCGGCGAATGCGGCCACTG
CCTGCATTCCGCTATCGGCGCAGGGCACGCAGTTGGGAATGCTGGCATTGCGTAGCGAAGACCCTGACGATCTTGCCTCG
TTGACCGTGGCCGAGGCGGCAGCGGAACAACTGTCCTTGGCGCTGCACAACCTGCGCTTGCGCGAAACGCTGCGCCAGCA
ATCGATCCGCGACCCGTTGACAGGCCTGTACAACCGCCGCTATCTGGAAGAAACCCTCAATCACGAGTTGGCGCGCTGCA
CGCGCCGGGCGCTGCCGTTGTCGGTACTGATGCTGGATGTGGATCACTTCAAGCGGTTCAACGACCTGCATGGGCACAGC
GGCGGCGACCGCGTACTGGCGGCCATTGGCGAATTGCTGCTGGCGCAGACGCGTGGCGAAGACATCTGCTGCCGGTACGG
CGGCGAAGAATTGACGATCATCCTGCCTGAGGTCGATCTGCCAACCGCCTGCATGATGGCGGAGAAACTGCGCGCCGGCA
TCGAGGCCCTGCAGGTCATGGGCGACGGCGTCTGCCTGCCGAAGGTCACCGCCTCGTTCGGCGCAGCCAGCTTTCCCGAT
CACGCCGGCACCGTGGCCTCGTTGTTGCAACGGGCGGACGCGGCGCTGTATCGCGCCAAGCAGGGTGGCCGCAATCAGGT
CGTGGGCGCGGATGTCCTTGCATTCGTGGCCTGA

Upstream 100 bases:

>100_bases
CATGACAGGTGAGTTGGTAATTGCCTAGAAAAATGACCAACGGGCGCCCTTTTCGGTGACTGCTTGCCAGCATACCGCCA
CGACGCTGCTTCCGGGGGCA

Downstream 100 bases:

>100_bases
ACGCAGGCAGCCGGGCGTGCTTTGTGCGGTCTGCCGTCAAGACTTGGGTTGCGCGGTCGACGCTCAGATCCGCATCGCGC
GGGCTTGGCGAACCCGGGTG

Product: putative sensor protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 597; Mature: 597

Protein sequence:

>597_residues
MCRLPADSASEVERRHAACIGLDQTIVPGNAVLTKRTKRRAAGASLVGGLIFVLIGVGGYLTTQRSLSDAGWVTHTQEVI
ASIDEIQAGMLSAESSARGYVLTDNEAFLGVYADAIGRLPERIVRLDALVQDNPTQRRNVVVLSRLVDARLAQIDELLHC
YRKQGLDGARAAIARGVFQTASSLRRQVQAMTELERQQLVRRNAATTRSAQWALWVTVIGIVSGLLVMLLAYRLLSRELE
RRTRAEDDASRTSERLIESFAVLERTSAGLEALARYSGLLQNCRDAEEALEITARTIAPLIPGAAGAVYLLRASRDRAEQ
VVAWGEMADDASTSIAPDNCWALRRDRTHVVMDASAGMLCQHAGVKLPANAATACIPLSAQGTQLGMLALRSEDPDDLAS
LTVAEAAAEQLSLALHNLRLRETLRQQSIRDPLTGLYNRRYLEETLNHELARCTRRALPLSVLMLDVDHFKRFNDLHGHS
GGDRVLAAIGELLLAQTRGEDICCRYGGEELTIILPEVDLPTACMMAEKLRAGIEALQVMGDGVCLPKVTASFGAASFPD
HAGTVASLLQRADAALYRAKQGGRNQVVGADVLAFVA

Sequences:

>Translated_597_residues
MCRLPADSASEVERRHAACIGLDQTIVPGNAVLTKRTKRRAAGASLVGGLIFVLIGVGGYLTTQRSLSDAGWVTHTQEVI
ASIDEIQAGMLSAESSARGYVLTDNEAFLGVYADAIGRLPERIVRLDALVQDNPTQRRNVVVLSRLVDARLAQIDELLHC
YRKQGLDGARAAIARGVFQTASSLRRQVQAMTELERQQLVRRNAATTRSAQWALWVTVIGIVSGLLVMLLAYRLLSRELE
RRTRAEDDASRTSERLIESFAVLERTSAGLEALARYSGLLQNCRDAEEALEITARTIAPLIPGAAGAVYLLRASRDRAEQ
VVAWGEMADDASTSIAPDNCWALRRDRTHVVMDASAGMLCQHAGVKLPANAATACIPLSAQGTQLGMLALRSEDPDDLAS
LTVAEAAAEQLSLALHNLRLRETLRQQSIRDPLTGLYNRRYLEETLNHELARCTRRALPLSVLMLDVDHFKRFNDLHGHS
GGDRVLAAIGELLLAQTRGEDICCRYGGEELTIILPEVDLPTACMMAEKLRAGIEALQVMGDGVCLPKVTASFGAASFPD
HAGTVASLLQRADAALYRAKQGGRNQVVGADVLAFVA
>Mature_597_residues
MCRLPADSASEVERRHAACIGLDQTIVPGNAVLTKRTKRRAAGASLVGGLIFVLIGVGGYLTTQRSLSDAGWVTHTQEVI
ASIDEIQAGMLSAESSARGYVLTDNEAFLGVYADAIGRLPERIVRLDALVQDNPTQRRNVVVLSRLVDARLAQIDELLHC
YRKQGLDGARAAIARGVFQTASSLRRQVQAMTELERQQLVRRNAATTRSAQWALWVTVIGIVSGLLVMLLAYRLLSRELE
RRTRAEDDASRTSERLIESFAVLERTSAGLEALARYSGLLQNCRDAEEALEITARTIAPLIPGAAGAVYLLRASRDRAEQ
VVAWGEMADDASTSIAPDNCWALRRDRTHVVMDASAGMLCQHAGVKLPANAATACIPLSAQGTQLGMLALRSEDPDDLAS
LTVAEAAAEQLSLALHNLRLRETLRQQSIRDPLTGLYNRRYLEETLNHELARCTRRALPLSVLMLDVDHFKRFNDLHGHS
GGDRVLAAIGELLLAQTRGEDICCRYGGEELTIILPEVDLPTACMMAEKLRAGIEALQVMGDGVCLPKVTASFGAASFPD
HAGTVASLLQRADAALYRAKQGGRNQVVGADVLAFVA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=167, Percent_Identity=37.125748502994, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI87081881, Length=174, Percent_Identity=39.0804597701149, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI145693134, Length=171, Percent_Identity=38.0116959064327, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI87082007, Length=173, Percent_Identity=36.9942196531792, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786584, Length=168, Percent_Identity=33.9285714285714, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1787816, Length=173, Percent_Identity=35.8381502890173, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1787802, Length=195, Percent_Identity=30.7692307692308, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1788085, Length=162, Percent_Identity=30.2469135802469, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788381, Length=170, Percent_Identity=31.1764705882353, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1787541, Length=169, Percent_Identity=27.810650887574, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI87081974, Length=173, Percent_Identity=24.2774566473988, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87081977, Length=184, Percent_Identity=29.3478260869565, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 64696; Mature: 64696

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCRLPADSASEVERRHAACIGLDQTIVPGNAVLTKRTKRRAAGASLVGGLIFVLIGVGGY
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
LTTQRSLSDAGWVTHTQEVIASIDEIQAGMLSAESSARGYVLTDNEAFLGVYADAIGRLP
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHH
ERIVRLDALVQDNPTQRRNVVVLSRLVDARLAQIDELLHCYRKQGLDGARAAIARGVFQT
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ASSLRRQVQAMTELERQQLVRRNAATTRSAQWALWVTVIGIVSGLLVMLLAYRLLSRELE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRTRAEDDASRTSERLIESFAVLERTSAGLEALARYSGLLQNCRDAEEALEITARTIAPL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPGAAGAVYLLRASRDRAEQVVAWGEMADDASTSIAPDNCWALRRDRTHVVMDASAGMLC
CCCCCCHHEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCEEEEEECCCCHHH
QHAGVKLPANAATACIPLSAQGTQLGMLALRSEDPDDLASLTVAEAAAEQLSLALHNLRL
HHCCCCCCCCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RETLRQQSIRDPLTGLYNRRYLEETLNHELARCTRRALPLSVLMLDVDHFKRFNDLHGHS
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
GGDRVLAAIGELLLAQTRGEDICCRYGGEELTIILPEVDLPTACMMAEKLRAGIEALQVM
CHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
GDGVCLPKVTASFGAASFPDHAGTVASLLQRADAALYRAKQGGRNQVVGADVLAFVA
CCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MCRLPADSASEVERRHAACIGLDQTIVPGNAVLTKRTKRRAAGASLVGGLIFVLIGVGGY
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
LTTQRSLSDAGWVTHTQEVIASIDEIQAGMLSAESSARGYVLTDNEAFLGVYADAIGRLP
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHH
ERIVRLDALVQDNPTQRRNVVVLSRLVDARLAQIDELLHCYRKQGLDGARAAIARGVFQT
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ASSLRRQVQAMTELERQQLVRRNAATTRSAQWALWVTVIGIVSGLLVMLLAYRLLSRELE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRTRAEDDASRTSERLIESFAVLERTSAGLEALARYSGLLQNCRDAEEALEITARTIAPL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPGAAGAVYLLRASRDRAEQVVAWGEMADDASTSIAPDNCWALRRDRTHVVMDASAGMLC
CCCCCCHHEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCEEEEEECCCCHHH
QHAGVKLPANAATACIPLSAQGTQLGMLALRSEDPDDLASLTVAEAAAEQLSLALHNLRL
HHCCCCCCCCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RETLRQQSIRDPLTGLYNRRYLEETLNHELARCTRRALPLSVLMLDVDHFKRFNDLHGHS
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
GGDRVLAAIGELLLAQTRGEDICCRYGGEELTIILPEVDLPTACMMAEKLRAGIEALQVM
CHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
GDGVCLPKVTASFGAASFPDHAGTVASLLQRADAALYRAKQGGRNQVVGADVLAFVA
CCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA