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The map label for this gene is folE

Identifier: 78045076

GI number: 78045076

Start: 842712

End: 843290

Strand: Direct

Name: folE

Synonym: CHY_0955

Alternate gene names: 78045076

Gene position: 842712-843290 (Clockwise)

Preceding gene: 78043751

Following gene: 78043012

Centisome position: 35.09

GC content: 49.05

Gene sequence:

>579_bases
ATGGTTGATAAAGAAAAAATTGAAATTGCCGTGCGGATGATTTTGGAAGCTATTGGAGAAGATCCGGACCGGGAAGGGCT
TAAGGATACTCCGAAAAGAGTTGCCCGGATGTATGAAGAAGTTTTTGCGGGACTGTCGCAGGACCCATCAGAACACCTGG
AACGGTATTTTACCGAAGAACACGAAGAGATGGTTCTGGTTAAGGACATTCCCCTGTATTCCATGTGTGAACATCACTTG
CTGCCATTTTACGGTAAAGCCCATGTGGCCTATATTCCGCGCAAGGGAAAGGTAACCGGTCTTTCCAAATTAGCCCGGGT
AGTGGAAGGTTTTGCCAAAAGACCGCAGCTTCAGGAAAGGCTTACCAGCCAAATTGCCGATGCTATCATGGAGAAGCTCA
ATCCGCGGGGAGTTTTGGTGGTTATTGAAGCGGAGCACATGTGTATGACCATGCGGGGGGTCAAAAAACCGGGTTCCAAG
ACCATTACTTCCGCGGTGCGGGGTATTTTTGCCACTTCGGTTGCCACCCGGGCGGAAGCAATGGCGTTAATTGGGCATCA
ATCACCCTTAAGGGATTAA

Upstream 100 bases:

>100_bases
CCAAAAGCAAAAAATGGCTTTAAAAAAGCTTTGGGATGTTCGGATAATTCCGCAGGTTCATAAACTTCTGCAGCTAAAAT
AAGGTATTGGAGGCGTTTTC

Downstream 100 bases:

>100_bases
TACGATATAATAAGAAAGAGGTGAGTTTAGTGAGCGAGAAAAAGGAAGGAAAGCTGGAACTCATAGCCCAGAAGCATTTT
ACACCCTGGGATGAAATGTA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MVDKEKIEIAVRMILEAIGEDPDREGLKDTPKRVARMYEEVFAGLSQDPSEHLERYFTEEHEEMVLVKDIPLYSMCEHHL
LPFYGKAHVAYIPRKGKVTGLSKLARVVEGFAKRPQLQERLTSQIADAIMEKLNPRGVLVVIEAEHMCMTMRGVKKPGSK
TITSAVRGIFATSVATRAEAMALIGHQSPLRD

Sequences:

>Translated_192_residues
MVDKEKIEIAVRMILEAIGEDPDREGLKDTPKRVARMYEEVFAGLSQDPSEHLERYFTEEHEEMVLVKDIPLYSMCEHHL
LPFYGKAHVAYIPRKGKVTGLSKLARVVEGFAKRPQLQERLTSQIADAIMEKLNPRGVLVVIEAEHMCMTMRGVKKPGSK
TITSAVRGIFATSVATRAEAMALIGHQSPLRD
>Mature_192_residues
MVDKEKIEIAVRMILEAIGEDPDREGLKDTPKRVARMYEEVFAGLSQDPSEHLERYFTEEHEEMVLVKDIPLYSMCEHHL
LPFYGKAHVAYIPRKGKVTGLSKLARVVEGFAKRPQLQERLTSQIADAIMEKLNPRGVLVVIEAEHMCMTMRGVKKPGSK
TITSAVRGIFATSVATRAEAMALIGHQSPLRD

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=176, Percent_Identity=52.8409090909091, Blast_Score=178, Evalue=3e-45,
Organism=Homo sapiens, GI4503949, Length=176, Percent_Identity=52.8409090909091, Blast_Score=178, Evalue=3e-45,
Organism=Homo sapiens, GI66932970, Length=150, Percent_Identity=48.6666666666667, Blast_Score=137, Evalue=7e-33,
Organism=Homo sapiens, GI66932972, Length=136, Percent_Identity=52.9411764705882, Blast_Score=136, Evalue=1e-32,
Organism=Escherichia coli, GI1788476, Length=179, Percent_Identity=41.8994413407821, Blast_Score=124, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17560486, Length=177, Percent_Identity=51.4124293785311, Blast_Score=181, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6321706, Length=186, Percent_Identity=49.4623655913978, Blast_Score=177, Evalue=7e-46,
Organism=Drosophila melanogaster, GI24656782, Length=177, Percent_Identity=49.7175141242938, Blast_Score=166, Evalue=7e-42,
Organism=Drosophila melanogaster, GI24656796, Length=177, Percent_Identity=49.7175141242938, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24656791, Length=177, Percent_Identity=49.7175141242938, Blast_Score=165, Evalue=2e-41,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_CARHZ (Q3ADI2)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359802.1
- ProteinModelPortal:   Q3ADI2
- SMR:   Q3ADI2
- STRING:   Q3ADI2
- GeneID:   3728202
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_0955
- NMPDR:   fig|246194.3.peg.432
- TIGR:   CHY_0955
- eggNOG:   COG0302
- HOGENOM:   HBG370191
- OMA:   ARIVEMF
- ProtClustDB:   PRK09347
- BioCyc:   CHYD246194:CHY_0955-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 21600; Mature: 21600

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDKEKIEIAVRMILEAIGEDPDREGLKDTPKRVARMYEEVFAGLSQDPSEHLERYFTEE
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
HEEMVLVKDIPLYSMCEHHLLPFYGKAHVAYIPRKGKVTGLSKLARVVEGFAKRPQLQER
HHCEEEEECCCHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHH
LTSQIADAIMEKLNPRGVLVVIEAEHMCMTMRGVKKPGSKTITSAVRGIFATSVATRAEA
HHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
MALIGHQSPLRD
HHHHHCCCCCCC
>Mature Secondary Structure
MVDKEKIEIAVRMILEAIGEDPDREGLKDTPKRVARMYEEVFAGLSQDPSEHLERYFTEE
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
HEEMVLVKDIPLYSMCEHHLLPFYGKAHVAYIPRKGKVTGLSKLARVVEGFAKRPQLQER
HHCEEEEECCCHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHH
LTSQIADAIMEKLNPRGVLVVIEAEHMCMTMRGVKKPGSKTITSAVRGIFATSVATRAEA
HHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
MALIGHQSPLRD
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA