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The map label for this gene is speE1 [H]

Identifier: 78045036

GI number: 78045036

Start: 1106673

End: 1107494

Strand: Direct

Name: speE1 [H]

Synonym: CHY_1240

Alternate gene names: 78045036

Gene position: 1106673-1107494 (Clockwise)

Preceding gene: 78043445

Following gene: 78043158

Centisome position: 46.08

GC content: 36.62

Gene sequence:

>822_bases
ATGTGGTATATTGAAAAACACAATGAAAATTATCAAGTTGGTTGGAGGGTTTCTGATATATTATATCAGAAAAAAACCCC
GTATCAAAACCTGGCAATTGTTGAATTTGCTGAATTCGGTAGATCCTTAATTTTAGATGATGCGGTCCAGACCACAGAAA
AAGATGAATACGTTTACCATGAAATGTTGGTTCATCCTGCAGCTTTTACCCATAAGTCTCCCCGTCGGGCCCTGATTATA
GGAGGTGGTGACGGAGGAACTTTAAGAGAAGTATTAAAACATAAAACTATTGAAAAAGTTGATTTAGTTGAAATAGATGA
AGAGGTTATCAAAGCTTCAAAGCAATTCTTACCATCTTTAAGTGAAAGTTTTAATGATCCCCGGGTTGAAGTTATTATTG
ATGATGGTATCCGTTACGTTAAAAAAGTAAAAAATTATTACGACCTGATCTTTGTGGATGCCTCAGACCCTGTGGGACCT
GCAGTGGTTTTATACAGTGAAGAATTTTACCGTTCCCTTTTTGATGCTTTAACTGCTGACGGAATAGCTGCGGTTCAATC
GGAATCGCCCAATTTCTACCCCGATATTTTCGTTAAAATCGTAACTACTTTGCGAGATATTTTTCCCAAGGTAAATGTCG
CTTTAGCTCCCGTTCCGTCATATATTAGCGGTTTCTTTGCTTTTACCGTTGCTTCAAAAGTTTATAATCCTAAAGAAACA
ACTCCCAACATTACGTTTCCTACTAAGTACTACAATGAAGAAATCCATAATGCTTGCTTTGCATTACCAAACTTTATCTT
AGATATGCTAAAAATCCGCTAA

Upstream 100 bases:

>100_bases
ATGACTTTTTCCTGTACCGCTGGGTCCTACAAAGCCTATTGTAGCCATTTTAGATACTCCTTCCTAATTAAAAAATATTT
AATTTGGAGGTGTTTTGCAA

Downstream 100 bases:

>100_bases
TTTTTAGCGGATTTTTTTATAAAAAAAGGAGTTTTAATTTTTAAACCGAATAAATTAAATAATGAGGGAAGGAAATGGAA
AGCAAAGTACTGGAAATGCT

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase 1; PAPT 1; SPDSY 1 [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MWYIEKHNENYQVGWRVSDILYQKKTPYQNLAIVEFAEFGRSLILDDAVQTTEKDEYVYHEMLVHPAAFTHKSPRRALII
GGGDGGTLREVLKHKTIEKVDLVEIDEEVIKASKQFLPSLSESFNDPRVEVIIDDGIRYVKKVKNYYDLIFVDASDPVGP
AVVLYSEEFYRSLFDALTADGIAAVQSESPNFYPDIFVKIVTTLRDIFPKVNVALAPVPSYISGFFAFTVASKVYNPKET
TPNITFPTKYYNEEIHNACFALPNFILDMLKIR

Sequences:

>Translated_273_residues
MWYIEKHNENYQVGWRVSDILYQKKTPYQNLAIVEFAEFGRSLILDDAVQTTEKDEYVYHEMLVHPAAFTHKSPRRALII
GGGDGGTLREVLKHKTIEKVDLVEIDEEVIKASKQFLPSLSESFNDPRVEVIIDDGIRYVKKVKNYYDLIFVDASDPVGP
AVVLYSEEFYRSLFDALTADGIAAVQSESPNFYPDIFVKIVTTLRDIFPKVNVALAPVPSYISGFFAFTVASKVYNPKET
TPNITFPTKYYNEEIHNACFALPNFILDMLKIR
>Mature_273_residues
MWYIEKHNENYQVGWRVSDILYQKKTPYQNLAIVEFAEFGRSLILDDAVQTTEKDEYVYHEMLVHPAAFTHKSPRRALII
GGGDGGTLREVLKHKTIEKVDLVEIDEEVIKASKQFLPSLSESFNDPRVEVIIDDGIRYVKKVKNYYDLIFVDASDPVGP
AVVLYSEEFYRSLFDALTADGIAAVQSESPNFYPDIFVKIVTTLRDIFPKVNVALAPVPSYISGFFAFTVASKVYNPKET
TPNITFPTKYYNEEIHNACFALPNFILDMLKIR

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=260, Percent_Identity=36.1538461538462, Blast_Score=193, Evalue=1e-49,
Organism=Escherichia coli, GI1786312, Length=278, Percent_Identity=35.6115107913669, Blast_Score=191, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI25154106, Length=264, Percent_Identity=42.0454545454545, Blast_Score=201, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI212645616, Length=218, Percent_Identity=43.5779816513761, Blast_Score=186, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6325326, Length=262, Percent_Identity=36.2595419847328, Blast_Score=199, Evalue=5e-52,
Organism=Saccharomyces cerevisiae, GI6323175, Length=263, Percent_Identity=38.0228136882129, Blast_Score=186, Evalue=3e-48,
Organism=Drosophila melanogaster, GI24645443, Length=257, Percent_Identity=38.9105058365759, Blast_Score=204, Evalue=6e-53,
Organism=Drosophila melanogaster, GI21355941, Length=203, Percent_Identity=39.9014778325123, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24663238, Length=235, Percent_Identity=25.1063829787234, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21358269, Length=235, Percent_Identity=25.1063829787234, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 31196; Mature: 31196

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWYIEKHNENYQVGWRVSDILYQKKTPYQNLAIVEFAEFGRSLILDDAVQTTEKDEYVYH
CCEEEECCCCEEEEEEHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCHHHHHH
EMLVHPAAFTHKSPRRALIIGGGDGGTLREVLKHKTIEKVDLVEIDEEVIKASKQFLPSL
HHHHCCHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
SESFNDPRVEVIIDDGIRYVKKVKNYYDLIFVDASDPVGPAVVLYSEEFYRSLFDALTAD
HHHCCCCEEEEEECCHHHHHHHHHHHHEEEEEECCCCCCCEEEEECHHHHHHHHHHHHHC
GIAAVQSESPNFYPDIFVKIVTTLRDIFPKVNVALAPVPSYISGFFAFTVASKVYNPKET
CHHEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCC
TPNITFPTKYYNEEIHNACFALPNFILDMLKIR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MWYIEKHNENYQVGWRVSDILYQKKTPYQNLAIVEFAEFGRSLILDDAVQTTEKDEYVYH
CCEEEECCCCEEEEEEHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCHHHHHH
EMLVHPAAFTHKSPRRALIIGGGDGGTLREVLKHKTIEKVDLVEIDEEVIKASKQFLPSL
HHHHCCHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
SESFNDPRVEVIIDDGIRYVKKVKNYYDLIFVDASDPVGPAVVLYSEEFYRSLFDALTAD
HHHCCCCEEEEEECCHHHHHHHHHHHHEEEEEECCCCCCCEEEEECHHHHHHHHHHHHHC
GIAAVQSESPNFYPDIFVKIVTTLRDIFPKVNVALAPVPSYISGFFAFTVASKVYNPKET
CHHEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCC
TPNITFPTKYYNEEIHNACFALPNFILDMLKIR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]