Click here to switch to the map view.

The map label for this gene is cspR [H]

Identifier: 78044914

GI number: 78044914

Start: 996081

End: 996542

Strand: Direct

Name: cspR [H]

Synonym: CHY_1122

Alternate gene names: 78044914

Gene position: 996081-996542 (Clockwise)

Preceding gene: 78043065

Following gene: 78043377

Centisome position: 41.48

GC content: 40.91

Gene sequence:

>462_bases
ATGTTTAACATTGTTTTGGTGGAACCGGAAATACCTTATAATACCGGAAATATTGCTCGCACCTGTGCTGCTACCGGTTC
AACCCTCCATCTGGTTAAACCCCTGGGTTTTTCCACTGAAGATAAATACTTAAAAAGAGCAGGGCTGGATTACTGGGACA
AAGTAAGGGTAATTTACCACGAAAACTTTCAGGAACTGAGGGAAAAATACCCGGAGGGTCGTTTTTTCTACCTTTCGACT
AAAGGCAAACGTTATTACCATGAGGTAAGCTATAAGCCTGGGGATTTTTTAGTGTTTGGGAAAGAGACACAGGGACTGCC
GCAGGAGCTTTTAGAAGCCAATAAAGAATTTACCTTTCGTATACCAATGTTGCCCCAAATAAGGTCGTTAAATTTATCAA
ATACCGTTGCCATTGTGGTTTACGAAGCTTTGCGTCAATTGCAGTTTCCAGATTTTGTTTAA

Upstream 100 bases:

>100_bases
CACCATCGCTTAATTTTTTTCGGAAGAAGAATATGTAAGGCCCAAAAGCCAAGCTGCAATATCTGTCCTTTTCCGGAATT
TTGCCAAAAGGAGGAGTCCG

Downstream 100 bases:

>100_bases
GGTGAATTTTATGGTGAAAAAAGCTAAAATGCGCCTTTGGCTGATGTTGGGCTTAACTTTTTGGGTATTGGCCTCCTGGT
TTATTTTAAATATCATCCAT

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 153; Mature: 153

Protein sequence:

>153_residues
MFNIVLVEPEIPYNTGNIARTCAATGSTLHLVKPLGFSTEDKYLKRAGLDYWDKVRVIYHENFQELREKYPEGRFFYLST
KGKRYYHEVSYKPGDFLVFGKETQGLPQELLEANKEFTFRIPMLPQIRSLNLSNTVAIVVYEALRQLQFPDFV

Sequences:

>Translated_153_residues
MFNIVLVEPEIPYNTGNIARTCAATGSTLHLVKPLGFSTEDKYLKRAGLDYWDKVRVIYHENFQELREKYPEGRFFYLST
KGKRYYHEVSYKPGDFLVFGKETQGLPQELLEANKEFTFRIPMLPQIRSLNLSNTVAIVVYEALRQLQFPDFV
>Mature_153_residues
MFNIVLVEPEIPYNTGNIARTCAATGSTLHLVKPLGFSTEDKYLKRAGLDYWDKVRVIYHENFQELREKYPEGRFFYLST
KGKRYYHEVSYKPGDFLVFGKETQGLPQELLEANKEFTFRIPMLPQIRSLNLSNTVAIVVYEALRQLQFPDFV

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=154, Percent_Identity=52.5974025974026, Blast_Score=162, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17865; Mature: 17865

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNIVLVEPEIPYNTGNIARTCAATGSTLHLVKPLGFSTEDKYLKRAGLDYWDKVRVIYH
CEEEEEECCCCCCCCCCHHHHHHCCCCEEEEEECCCCCCHHHHHHHCCCCHHHHEEEEEC
ENFQELREKYPEGRFFYLSTKGKRYYHEVSYKPGDFLVFGKETQGLPQELLEANKEFTFR
CCHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCEEEECCCCCCCCHHHHHCCCCEEEE
IPMLPQIRSLNLSNTVAIVVYEALRQLQFPDFV
CCCCCCHHCCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFNIVLVEPEIPYNTGNIARTCAATGSTLHLVKPLGFSTEDKYLKRAGLDYWDKVRVIYH
CEEEEEECCCCCCCCCCHHHHHHCCCCEEEEEECCCCCCHHHHHHHCCCCHHHHEEEEEC
ENFQELREKYPEGRFFYLSTKGKRYYHEVSYKPGDFLVFGKETQGLPQELLEANKEFTFR
CCHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCEEEECCCCCCCCHHHHHCCCCEEEE
IPMLPQIRSLNLSNTVAIVVYEALRQLQFPDFV
CCCCCCHHCCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]