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The map label for this gene is aksA [H]

Identifier: 78044757

GI number: 78044757

Start: 980104

End: 981246

Strand: Direct

Name: aksA [H]

Synonym: CHY_1104

Alternate gene names: 78044757

Gene position: 980104-981246 (Clockwise)

Preceding gene: 78043368

Following gene: 78043811

Centisome position: 40.81

GC content: 46.81

Gene sequence:

>1143_bases
GTGAAATTAAAAGATAAACCAATTTATATCGTTGATACGACCCTAAGAGATGGAGAACAAACCGCAGGAGTAGTTTTTGC
AAATAAGGAAAAAATTCGAATTGCCCAGATGCTGGATGAACTTGGTGTTGACCAGATTGAGGCCGGTATTCCCACGATGG
GTGGCGATGAAAAAGAGGCAATTACCGCCATCTGTAAACTTGGACTAAAAGCCAGTATCATGGGCTGGAACCGTCCGGTA
ATTAAAGATATTGAAGAGTCGCTGCGCTGCGGGGTAGATGCGGTTGCTATATCTATTTCTACCTCGGATATCCATATTGA
ACATAAACTCCGGGCCTCCCGGGAATGGGTTATTGAAAATATGGTTAAAGCCTGCGAGTTTGCTAAAAAAGAAGGTTTGT
ACGTTTCGGTGAACGCTGAGGATGCCAGCCGCTCCGACATGGAATTTTTAGTGCAGTTTGGTTTGGCGGCGAAACAAGCG
GGAGCCGACCGTTTAAGATATTGCGATACCGTGGGTATCCTTGATCCCTTTACTACTTATGAAAGGGTCAAGGAATTGCG
GGAAAGAGTAGGTATTGAAATTGAAATGCATACCCACAACGACTTTGGCATGGCCACGGCCAATGCTTTAGCCGGGGTTA
AAGCCGGAGCTAAGTTTGTAGGGGTTACGGTTATTGGCCTTGGAGAGCGGGCCGGAAACGCTGCTTTAGAAGAAGTGGTA
ATGGCCTTAAAACATATTTACGGTATCGATCTTGGCTTTAAGACCGAGCTTTTCCGGGAAGTTGCTCTTTATGTTTCCAA
GGCTTCCGGCCGGGAACTTCCCGCCTGGAAAGCTATTGTGGGTTCCAACATGTTTGCCCACGAATCGGGAATCCATGCCG
ATGGAGCGTTAAAGAATCCTAAAACCTACGAGGCTTTCCAGCCCGAAGAAGTGGGTCTGGAGCGACAAATTGTTATCGGT
AAGCACTCCGGTTCGGCAGCGTTAAAAGCGAAATTTGCCGAGTACGGAATTCACTTAACCGATGAACAGGCAGCAGAGCT
TCTACCCCATGTCCGGGCCCATGCGGTGGAATTAAAACGGTCTTTGTTTGATAAAGAGCTTGTTTTAATCTACGAAGAAC
ATTTCGGAAAGCGGGGAATGTAG

Upstream 100 bases:

>100_bases
TAAGTATACAGTATAAAAACTTTGAAAATCTTTTAACAATTTTGGGGGTATGTTATCATTAAAAAATAGATTAAGCTACA
AAGTGGGAGGGTGAAATAAC

Downstream 100 bases:

>100_bases
CTTTGGGAAAAACAATCATTGAAAAAATCCTTTCGCAAAAAAGCGGCGTTGATGCTTATGCGGGCCAAATTGTGGTGGCC
GATGTGGATTACGTTATGGG

Product: trans-homoaconitate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2 [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MKLKDKPIYIVDTTLRDGEQTAGVVFANKEKIRIAQMLDELGVDQIEAGIPTMGGDEKEAITAICKLGLKASIMGWNRPV
IKDIEESLRCGVDAVAISISTSDIHIEHKLRASREWVIENMVKACEFAKKEGLYVSVNAEDASRSDMEFLVQFGLAAKQA
GADRLRYCDTVGILDPFTTYERVKELRERVGIEIEMHTHNDFGMATANALAGVKAGAKFVGVTVIGLGERAGNAALEEVV
MALKHIYGIDLGFKTELFREVALYVSKASGRELPAWKAIVGSNMFAHESGIHADGALKNPKTYEAFQPEEVGLERQIVIG
KHSGSAALKAKFAEYGIHLTDEQAAELLPHVRAHAVELKRSLFDKELVLIYEEHFGKRGM

Sequences:

>Translated_380_residues
MKLKDKPIYIVDTTLRDGEQTAGVVFANKEKIRIAQMLDELGVDQIEAGIPTMGGDEKEAITAICKLGLKASIMGWNRPV
IKDIEESLRCGVDAVAISISTSDIHIEHKLRASREWVIENMVKACEFAKKEGLYVSVNAEDASRSDMEFLVQFGLAAKQA
GADRLRYCDTVGILDPFTTYERVKELRERVGIEIEMHTHNDFGMATANALAGVKAGAKFVGVTVIGLGERAGNAALEEVV
MALKHIYGIDLGFKTELFREVALYVSKASGRELPAWKAIVGSNMFAHESGIHADGALKNPKTYEAFQPEEVGLERQIVIG
KHSGSAALKAKFAEYGIHLTDEQAAELLPHVRAHAVELKRSLFDKELVLIYEEHFGKRGM
>Mature_380_residues
MKLKDKPIYIVDTTLRDGEQTAGVVFANKEKIRIAQMLDELGVDQIEAGIPTMGGDEKEAITAICKLGLKASIMGWNRPV
IKDIEESLRCGVDAVAISISTSDIHIEHKLRASREWVIENMVKACEFAKKEGLYVSVNAEDASRSDMEFLVQFGLAAKQA
GADRLRYCDTVGILDPFTTYERVKELRERVGIEIEMHTHNDFGMATANALAGVKAGAKFVGVTVIGLGERAGNAALEEVV
MALKHIYGIDLGFKTELFREVALYVSKASGRELPAWKAIVGSNMFAHESGIHADGALKNPKTYEAFQPEEVGLERQIVIG
KHSGSAALKAKFAEYGIHLTDEQAAELLPHVRAHAVELKRSLFDKELVLIYEEHFGKRGM

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=347, Percent_Identity=38.328530259366, Blast_Score=199, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6320019, Length=349, Percent_Identity=33.5243553008596, Blast_Score=202, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6320071, Length=353, Percent_Identity=33.1444759206799, Blast_Score=202, Evalue=8e-53,
Organism=Drosophila melanogaster, GI24582381, Length=285, Percent_Identity=27.719298245614, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 41799; Mature: 41799

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKDKPIYIVDTTLRDGEQTAGVVFANKEKIRIAQMLDELGVDQIEAGIPTMGGDEKEA
CCCCCCCEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHCHHHHHCCCCCCCCCHHHH
ITAICKLGLKASIMGWNRPVIKDIEESLRCGVDAVAISISTSDIHIEHKLRASREWVIEN
HHHHHHHCCCEEECCCCCHHHHHHHHHHHCCCEEEEEEEECCEEEEHHHHHHHHHHHHHH
MVKACEFAKKEGLYVSVNAEDASRSDMEFLVQFGLAAKQAGADRLRYCDTVGILDPFTTY
HHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHH
ERVKELRERVGIEIEMHTHNDFGMATANALAGVKAGAKFVGVTVIGLGERAGNAALEEVV
HHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHH
MALKHIYGIDLGFKTELFREVALYVSKASGRELPAWKAIVGSNMFAHESGIHADGALKNP
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCEECCCCCCCCCCCCCC
KTYEAFQPEEVGLERQIVIGKHSGSAALKAKFAEYGIHLTDEQAAELLPHVRAHAVELKR
CCCCCCCCHHCCCCEEEEEEECCCCHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHH
SLFDKELVLIYEEHFGKRGM
HHCCCCEEEEEEHHCCCCCC
>Mature Secondary Structure
MKLKDKPIYIVDTTLRDGEQTAGVVFANKEKIRIAQMLDELGVDQIEAGIPTMGGDEKEA
CCCCCCCEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHCHHHHHCCCCCCCCCHHHH
ITAICKLGLKASIMGWNRPVIKDIEESLRCGVDAVAISISTSDIHIEHKLRASREWVIEN
HHHHHHHCCCEEECCCCCHHHHHHHHHHHCCCEEEEEEEECCEEEEHHHHHHHHHHHHHH
MVKACEFAKKEGLYVSVNAEDASRSDMEFLVQFGLAAKQAGADRLRYCDTVGILDPFTTY
HHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHH
ERVKELRERVGIEIEMHTHNDFGMATANALAGVKAGAKFVGVTVIGLGERAGNAALEEVV
HHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHH
MALKHIYGIDLGFKTELFREVALYVSKASGRELPAWKAIVGSNMFAHESGIHADGALKNP
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCEECCCCCCCCCCCCCC
KTYEAFQPEEVGLERQIVIGKHSGSAALKAKFAEYGIHLTDEQAAELLPHVRAHAVELKR
CCCCCCCCHHCCCCEEEEEEECCCCHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHH
SLFDKELVLIYEEHFGKRGM
HHCCCCEEEEEEHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]