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The map label for this gene is gatB

Identifier: 78043368

GI number: 78043368

Start: 978478

End: 979923

Strand: Direct

Name: gatB

Synonym: CHY_1103

Alternate gene names: 78043368

Gene position: 978478-979923 (Clockwise)

Preceding gene: 78044168

Following gene: 78044757

Centisome position: 40.74

GC content: 45.64

Gene sequence:

>1446_bases
ATGCGCGAATACGAAGCGGTTATTGGGTTAGAAGTTCATGTGGAGTTAAAAACCAATACTAAAATCTTTTGCAATTGTTC
TACCGAGTTTGGCGGCGATCCGAACTCGCACGTTTGTCCGGTGTGCTTGGGGCTTCCCGGGGTTTTGCCCGTTTTAAATA
AAAAAGTGGTGGATTACGCGATTAAAGCAGCGCTGGCGTTAAACTGCGAAGTGGCCGAATACTGCAAATTTGACCGGAAA
AATTACTACTACCCCGACTTACCCAAGAATTATCAGATATCCCAGTACGATTTGCCGTTAGCGAAAAACGGTTATTTAGA
AATTGAGGTCGATGGGCAGGTAAAAAGAATTGGCATTACCCGGATTCACATGGAAGAAGATGCGGGAAAACTGGTACACC
AGGGAAACCTTGCGGTAACACCTTACTCTTTAGTTGACTACAATCGAACCGGAGTGCCGCTGATTGAAATAGTTTCGGCG
CCGGATATCCGTACTCCCGAGGAAGCAAGACTTTACTTGGAAAAACTTAAAGCAATTATCCAGTATACCGGAATTTCCGA
CTGCCGGATGGAGGAAGGAAGTCTCAGGTGTGATGCGAACGTTTCCGTTCGTCCCAAAGGCCAGAAAGAGTTCGGAACCA
AAACGGAAATAAAAAATATGAACTCTTTTAAAGCTCTGCAAAAAGCTTTGGAATACGAAATTGCCCGGCAAATTGAGATT
TTAGAGGAAGGCGGGCGAATCATCCAGGAAACCCGGATGTGGGATGAGAGCCGTCAGGTAACCCTTTCCATGCGCAGCAA
AGAGGAAGCCCACGATTACCGTTATTTCCCCGAGCCGGATTTACCGCCGGTGGTCATTGACCGGGAGTGGTTAGAAGAGA
TAAGGGCTACATTGCCGGAACTTCCAGATGCTCGCAAGAAACGGTTTGTGGAACAGTATGGCTTAACTCCCTACGATGCC
CATGTCCTGACCTTAACCCGGGAACTGGCGGATTACTACGAGGAAGCGGCGAAGGGTTACAGTAATCCAAAAGCAGTAGC
TAACTGGGTCATAAATGAGCTTTTACGTTTACTAAATGCTGCAGGAAAAGAAATTACCGAATGTCCCATAAAACCTGAAC
AAATTACCAAGATGCTGGAACTTTTAGATAAGGGCACAATTTCCGGTAAAATTGCCAAAACGGTGTTTGAAGAGATGTTT
TCATCCGGTAAAGACCCGGAGGTAATCGTCAAAGAAAAAGGACTGGTGCAAATTACCGATTTGGGTGAATTGGAAGCGGT
GGTGGATAAAGTAATTGCAGCAAATCCCAAAGCGGTGGAAGATTACAAGAACGGAAAGGAAAAGGCATTAGGCTTCTTAG
TTGGTCAGATTATGAAAGAAACCCGCGGACGGGCTAATCCTGAAGCGGTAAACCAGCTCTTACGGGACAAATTAGCAAAG
CTATAA

Upstream 100 bases:

>100_bases
GGCAAGCCTTTTGATGAAGGAACCTTACTGAAAGTTGCCCATGCCTTTGAGCAAAATACCGAATTTCATAAGGCACGGCC
CAAGCTTTAGGAGGTGTATT

Downstream 100 bases:

>100_bases
TATAAAGACCGCTGTCTTACCGGCAGCGGCTTTTTTTTGATTTTCTACTGACCACGGACCACCTACCACTTACCACTGTT
TAAGTATACAGTATAAAAAC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MREYEAVIGLEVHVELKTNTKIFCNCSTEFGGDPNSHVCPVCLGLPGVLPVLNKKVVDYAIKAALALNCEVAEYCKFDRK
NYYYPDLPKNYQISQYDLPLAKNGYLEIEVDGQVKRIGITRIHMEEDAGKLVHQGNLAVTPYSLVDYNRTGVPLIEIVSA
PDIRTPEEARLYLEKLKAIIQYTGISDCRMEEGSLRCDANVSVRPKGQKEFGTKTEIKNMNSFKALQKALEYEIARQIEI
LEEGGRIIQETRMWDESRQVTLSMRSKEEAHDYRYFPEPDLPPVVIDREWLEEIRATLPELPDARKKRFVEQYGLTPYDA
HVLTLTRELADYYEEAAKGYSNPKAVANWVINELLRLLNAAGKEITECPIKPEQITKMLELLDKGTISGKIAKTVFEEMF
SSGKDPEVIVKEKGLVQITDLGELEAVVDKVIAANPKAVEDYKNGKEKALGFLVGQIMKETRGRANPEAVNQLLRDKLAK
L

Sequences:

>Translated_481_residues
MREYEAVIGLEVHVELKTNTKIFCNCSTEFGGDPNSHVCPVCLGLPGVLPVLNKKVVDYAIKAALALNCEVAEYCKFDRK
NYYYPDLPKNYQISQYDLPLAKNGYLEIEVDGQVKRIGITRIHMEEDAGKLVHQGNLAVTPYSLVDYNRTGVPLIEIVSA
PDIRTPEEARLYLEKLKAIIQYTGISDCRMEEGSLRCDANVSVRPKGQKEFGTKTEIKNMNSFKALQKALEYEIARQIEI
LEEGGRIIQETRMWDESRQVTLSMRSKEEAHDYRYFPEPDLPPVVIDREWLEEIRATLPELPDARKKRFVEQYGLTPYDA
HVLTLTRELADYYEEAAKGYSNPKAVANWVINELLRLLNAAGKEITECPIKPEQITKMLELLDKGTISGKIAKTVFEEMF
SSGKDPEVIVKEKGLVQITDLGELEAVVDKVIAANPKAVEDYKNGKEKALGFLVGQIMKETRGRANPEAVNQLLRDKLAK
L
>Mature_481_residues
MREYEAVIGLEVHVELKTNTKIFCNCSTEFGGDPNSHVCPVCLGLPGVLPVLNKKVVDYAIKAALALNCEVAEYCKFDRK
NYYYPDLPKNYQISQYDLPLAKNGYLEIEVDGQVKRIGITRIHMEEDAGKLVHQGNLAVTPYSLVDYNRTGVPLIEIVSA
PDIRTPEEARLYLEKLKAIIQYTGISDCRMEEGSLRCDANVSVRPKGQKEFGTKTEIKNMNSFKALQKALEYEIARQIEI
LEEGGRIIQETRMWDESRQVTLSMRSKEEAHDYRYFPEPDLPPVVIDREWLEEIRATLPELPDARKKRFVEQYGLTPYDA
HVLTLTRELADYYEEAAKGYSNPKAVANWVINELLRLLNAAGKEITECPIKPEQITKMLELLDKGTISGKIAKTVFEEMF
SSGKDPEVIVKEKGLVQITDLGELEAVVDKVIAANPKAVEDYKNGKEKALGFLVGQIMKETRGRANPEAVNQLLRDKLAK
L

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=501, Percent_Identity=40.5189620758483, Blast_Score=347, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI115534094, Length=473, Percent_Identity=32.9809725158562, Blast_Score=239, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6319391, Length=511, Percent_Identity=31.8982387475538, Blast_Score=234, Evalue=2e-62,
Organism=Drosophila melanogaster, GI45550808, Length=497, Percent_Identity=36.6197183098592, Blast_Score=310, Evalue=2e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_CARHZ (Q3AD35)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359949.1
- ProteinModelPortal:   Q3AD35
- SMR:   Q3AD35
- STRING:   Q3AD35
- GeneID:   3728889
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_1103
- NMPDR:   fig|246194.3.peg.1150
- TIGR:   CHY_1103
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   CHYD246194:CHY_1103-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 54405; Mature: 54405

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREYEAVIGLEVHVELKTNTKIFCNCSTEFGGDPNSHVCPVCLGLPGVLPVLNKKVVDYA
CCCCCEEEEEEEEEEEECCCEEEEEECCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHH
IKAALALNCEVAEYCKFDRKNYYYPDLPKNYQISQYDLPLAKNGYLEIEVDGQVKRIGIT
HHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEE
RIHMEEDAGKLVHQGNLAVTPYSLVDYNRTGVPLIEIVSAPDIRTPEEARLYLEKLKAII
EEEECCCCCHHEECCCEEECCHHHCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHH
QYTGISDCRMEEGSLRCDANVSVRPKGQKEFGTKTEIKNMNSFKALQKALEYEIARQIEI
HHCCCCCCCCCCCCEEECCCCEECCCCCHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHH
LEEGGRIIQETRMWDESRQVTLSMRSKEEAHDYRYFPEPDLPPVVIDREWLEEIRATLPE
HHHCCHHHHHHHHCCCCCEEEEEECCCHHHHCCCCCCCCCCCCEEECHHHHHHHHHHCCC
LPDARKKRFVEQYGLTPYDAHVLTLTRELADYYEEAAKGYSNPKAVANWVINELLRLLNA
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
AGKEITECPIKPEQITKMLELLDKGTISGKIAKTVFEEMFSSGKDPEVIVKEKGLVQITD
CCCHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEC
LGELEAVVDKVIAANPKAVEDYKNGKEKALGFLVGQIMKETRGRANPEAVNQLLRDKLAK
CHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHC
L
C
>Mature Secondary Structure
MREYEAVIGLEVHVELKTNTKIFCNCSTEFGGDPNSHVCPVCLGLPGVLPVLNKKVVDYA
CCCCCEEEEEEEEEEEECCCEEEEEECCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHH
IKAALALNCEVAEYCKFDRKNYYYPDLPKNYQISQYDLPLAKNGYLEIEVDGQVKRIGIT
HHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEE
RIHMEEDAGKLVHQGNLAVTPYSLVDYNRTGVPLIEIVSAPDIRTPEEARLYLEKLKAII
EEEECCCCCHHEECCCEEECCHHHCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHH
QYTGISDCRMEEGSLRCDANVSVRPKGQKEFGTKTEIKNMNSFKALQKALEYEIARQIEI
HHCCCCCCCCCCCCEEECCCCEECCCCCHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHH
LEEGGRIIQETRMWDESRQVTLSMRSKEEAHDYRYFPEPDLPPVVIDREWLEEIRATLPE
HHHCCHHHHHHHHCCCCCEEEEEECCCHHHHCCCCCCCCCCCCEEECHHHHHHHHHHCCC
LPDARKKRFVEQYGLTPYDAHVLTLTRELADYYEEAAKGYSNPKAVANWVINELLRLLNA
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
AGKEITECPIKPEQITKMLELLDKGTISGKIAKTVFEEMFSSGKDPEVIVKEKGLVQITD
CCCHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEC
LGELEAVVDKVIAANPKAVEDYKNGKEKALGFLVGQIMKETRGRANPEAVNQLLRDKLAK
CHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA