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The map label for this gene is rumA [C]

Identifier: 78044405

GI number: 78044405

Start: 987260

End: 988585

Strand: Direct

Name: rumA [C]

Synonym: CHY_1112

Alternate gene names: 78044405

Gene position: 987260-988585 (Clockwise)

Preceding gene: 78044064

Following gene: 78045161

Centisome position: 41.11

GC content: 40.87

Gene sequence:

>1326_bases
ATGGAAGAAATTTTAGAAATAATTGACCTTACCGAAGAAGGACTGGGTGTTGGGAAAAAAGAAAAGCGGGTTTACTTTGT
TTCGGGGGATGTCACCGTTGGTGATGTGGTCAAAGTAAAAGTTACCAGCGAAAAAAGAGGCGTTATTCTGGGAGAGGCTC
TGGAAATTTTAACTAATTCTCCTTACCGGGATGTTCCCAGGTGTCCCCATTTTGGTTACTGCGGAGGTTGCAGTTTACAA
AACCTTGATTACGAGCAGCAGTTAGTACTTAAAAAAACAAAAGTTTATAATATTATGAAAAAAATAGGCCGGATAGATGC
TCCTATCAACTCCGTTCTACCTAATTTTGCTTTTTACCGCTACCGTCAAAAAGTGCATTTCACTTTTGGAAGTAGCCAGA
ATAAACTGGCTATAGGTTATTTTGACCGAAAAAATAACTGGTTTCCTGTAACCGATTGTCTGATTGCCGATAAAAGCTTA
GTTGAGGTGGCTCATTATGTTACTGAAATATTAGATGCTTTTAAGCTTAAAGCTTACTTTAACGATGCTGGAGTGCTACG
GCATTTGGTGGTTCGTAAGTCATCAACTACCGGTGAAATCCAGGTAGTGATAACAGCCGTAAAATTAAATTTTAACGTAA
AAGAAATTGCTGCGAAAATTCTTTTAAACCCTGAAATCCAGTCGCTAACTTTTCATGAAAACCCCAAAAAAACCAGGGAA
GTCTTTGGTAAAGGGACAGCCGTTACTTTTGGTACCCGGTATATTACCGAAGAACTATTAGGGATTAGCTATAGGCTAAC
TCCTCTTTCCTTTTTTCAAATAAATCCGGTGCAGGCGGAAAAGATATACCGCATTGCCTTAGATTATCTTGATTTGGGAA
AAACCGATATGGTTTTTGACGGCTATTCGGGAGTGGGATCCCTGACCTTACCGGCTGCCCGAATTGCCGCAAGCGTTGTA
GGAGTGGAAGAAATTAGGGATGCGGTAGAGGTGGCCAAGACCAATGCTAAACTAAACGGTATAGCGAATGTTAATTTTTT
TGCCGGAAAAGTGGAAGAAAAACTTCCCTGGCTTTTAAAGAAAGGGTATCGTTTTAATAAAATTATCCTGGATCCTCCCC
GGGAGGGAGTAAAAAAAGAGGTTTTAGAGGAGATAATAAAGCTCAATCCGGAACGGATTGTTTATGTTTCATGCAATCCT
GCAACCCAGGCCCGGGATTTAAAGATTCTGGCGGAAACCGGCTATTACCCGGTGGAAATAACACCGGTGGACATGTTTTC
CCAGACGATGCACGTGGAGAACGTGGTATTGCTCAAGAAAAAATAA

Upstream 100 bases:

>100_bases
GATATGAATAGCCCGTTAGATGAAAAGTTTCGGCAGAGGATTGAGGAGCAGGGTATAGAGATTTACCGGAGAGAATAGAA
AGTTTAATTCAGGAGGATTT

Downstream 100 bases:

>100_bases
CCCCTAAGGCCTTGCCGAGCAAGGCCTTAGGGGTCTTACTTTTCAAAAGCTGCCAAAGAAGTGACTAGGGTTGAGTTTGC
TTTGGTGAAGTAAGTAATCC

Product: 23S rRNA (uracil-5-)-methyltransferase RumA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MEEILEIIDLTEEGLGVGKKEKRVYFVSGDVTVGDVVKVKVTSEKRGVILGEALEILTNSPYRDVPRCPHFGYCGGCSLQ
NLDYEQQLVLKKTKVYNIMKKIGRIDAPINSVLPNFAFYRYRQKVHFTFGSSQNKLAIGYFDRKNNWFPVTDCLIADKSL
VEVAHYVTEILDAFKLKAYFNDAGVLRHLVVRKSSTTGEIQVVITAVKLNFNVKEIAAKILLNPEIQSLTFHENPKKTRE
VFGKGTAVTFGTRYITEELLGISYRLTPLSFFQINPVQAEKIYRIALDYLDLGKTDMVFDGYSGVGSLTLPAARIAASVV
GVEEIRDAVEVAKTNAKLNGIANVNFFAGKVEEKLPWLLKKGYRFNKIILDPPREGVKKEVLEEIIKLNPERIVYVSCNP
ATQARDLKILAETGYYPVEITPVDMFSQTMHVENVVLLKKK

Sequences:

>Translated_441_residues
MEEILEIIDLTEEGLGVGKKEKRVYFVSGDVTVGDVVKVKVTSEKRGVILGEALEILTNSPYRDVPRCPHFGYCGGCSLQ
NLDYEQQLVLKKTKVYNIMKKIGRIDAPINSVLPNFAFYRYRQKVHFTFGSSQNKLAIGYFDRKNNWFPVTDCLIADKSL
VEVAHYVTEILDAFKLKAYFNDAGVLRHLVVRKSSTTGEIQVVITAVKLNFNVKEIAAKILLNPEIQSLTFHENPKKTRE
VFGKGTAVTFGTRYITEELLGISYRLTPLSFFQINPVQAEKIYRIALDYLDLGKTDMVFDGYSGVGSLTLPAARIAASVV
GVEEIRDAVEVAKTNAKLNGIANVNFFAGKVEEKLPWLLKKGYRFNKIILDPPREGVKKEVLEEIIKLNPERIVYVSCNP
ATQARDLKILAETGYYPVEITPVDMFSQTMHVENVVLLKKK
>Mature_441_residues
MEEILEIIDLTEEGLGVGKKEKRVYFVSGDVTVGDVVKVKVTSEKRGVILGEALEILTNSPYRDVPRCPHFGYCGGCSLQ
NLDYEQQLVLKKTKVYNIMKKIGRIDAPINSVLPNFAFYRYRQKVHFTFGSSQNKLAIGYFDRKNNWFPVTDCLIADKSL
VEVAHYVTEILDAFKLKAYFNDAGVLRHLVVRKSSTTGEIQVVITAVKLNFNVKEIAAKILLNPEIQSLTFHENPKKTRE
VFGKGTAVTFGTRYITEELLGISYRLTPLSFFQINPVQAEKIYRIALDYLDLGKTDMVFDGYSGVGSLTLPAARIAASVV
GVEEIRDAVEVAKTNAKLNGIANVNFFAGKVEEKLPWLLKKGYRFNKIILDPPREGVKKEVLEEIIKLNPERIVYVSCNP
ATQARDLKILAETGYYPVEITPVDMFSQTMHVENVVLLKKK

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=200, Percent_Identity=36, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI51173878, Length=200, Percent_Identity=36, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI269784680, Length=399, Percent_Identity=27.0676691729323, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI269784676, Length=399, Percent_Identity=27.0676691729323, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI20149688, Length=399, Percent_Identity=27.0676691729323, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI269784678, Length=309, Percent_Identity=28.8025889967638, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI1789148, Length=440, Percent_Identity=31.5909090909091, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1787083, Length=377, Percent_Identity=23.342175066313, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1790403, Length=382, Percent_Identity=26.7015706806283, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6322909, Length=384, Percent_Identity=25.5208333333333, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24666579, Length=420, Percent_Identity=28.0952380952381, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 49644; Mature: 49644

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEILEIIDLTEEGLGVGKKEKRVYFVSGDVTVGDVVKVKVTSEKRGVILGEALEILTNS
CHHHHHHHHCCHHHCCCCCCCCEEEEEECCEEECCEEEEEEECCCCCEEHHHHHHHHCCC
PYRDVPRCPHFGYCGGCSLQNLDYEQQLVLKKTKVYNIMKKIGRIDAPINSVLPNFAFYR
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHH
YRQKVHFTFGSSQNKLAIGYFDRKNNWFPVTDCLIADKSLVEVAHYVTEILDAFKLKAYF
EEEEEEEEECCCCCEEEEEEEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
NDAGVLRHLVVRKSSTTGEIQVVITAVKLNFNVKEIAAKILLNPEIQSLTFHENPKKTRE
CHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCHHHHHHHHHCCCCCCEEEECCCCHHHHH
VFGKGTAVTFGTRYITEELLGISYRLTPLSFFQINPVQAEKIYRIALDYLDLGKTDMVFD
HHCCCCEEEECHHHHHHHHHCCEEEECEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEE
GYSGVGSLTLPAARIAASVVGVEEIRDAVEVAKTNAKLNGIANVNFFAGKVEEKLPWLLK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHCCHHHH
KGYRFNKIILDPPREGVKKEVLEEIIKLNPERIVYVSCNPATQARDLKILAETGYYPVEI
CCCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEECCCEEEEE
TPVDMFSQTMHVENVVLLKKK
EEHHHHHHHHHHCEEEEEECC
>Mature Secondary Structure
MEEILEIIDLTEEGLGVGKKEKRVYFVSGDVTVGDVVKVKVTSEKRGVILGEALEILTNS
CHHHHHHHHCCHHHCCCCCCCCEEEEEECCEEECCEEEEEEECCCCCEEHHHHHHHHCCC
PYRDVPRCPHFGYCGGCSLQNLDYEQQLVLKKTKVYNIMKKIGRIDAPINSVLPNFAFYR
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHH
YRQKVHFTFGSSQNKLAIGYFDRKNNWFPVTDCLIADKSLVEVAHYVTEILDAFKLKAYF
EEEEEEEEECCCCCEEEEEEEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
NDAGVLRHLVVRKSSTTGEIQVVITAVKLNFNVKEIAAKILLNPEIQSLTFHENPKKTRE
CHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCHHHHHHHHHCCCCCCEEEECCCCHHHHH
VFGKGTAVTFGTRYITEELLGISYRLTPLSFFQINPVQAEKIYRIALDYLDLGKTDMVFD
HHCCCCEEEECHHHHHHHHHCCEEEECEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEE
GYSGVGSLTLPAARIAASVVGVEEIRDAVEVAKTNAKLNGIANVNFFAGKVEEKLPWLLK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHCCHHHH
KGYRFNKIILDPPREGVKKEVLEEIIKLNPERIVYVSCNPATQARDLKILAETGYYPVEI
CCCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEECCCEEEEE
TPVDMFSQTMHVENVVLLKKK
EEHHHHHHHHHHCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]