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The map label for this gene is dacB [H]

Identifier: 78044170

GI number: 78044170

Start: 1020861

End: 1021838

Strand: Direct

Name: dacB [H]

Synonym: CHY_1147

Alternate gene names: 78044170

Gene position: 1020861-1021838 (Clockwise)

Preceding gene: 78043651

Following gene: 78043889

Centisome position: 42.51

GC content: 37.32

Gene sequence:

>978_bases
ATGAAGAGATATTTGATTCCATTGCTTTTATGTTTGTTAACGTTACCGGTAAAAGTCCATGGGGAAATAAAGGCAAAAGC
TTATTACTTTTTTATACCAACTACTAATCAAATCATTGAGGCCAAAAATCAAGACTTTCCCCTACCACCGGCAAGTACCA
CCAAAATTGCTACCGCTTTAACAGCATTGGACCTTTATCCGAGGAATTTTACCTTAACGGTAAAACCAGAAGCGGTGCGG
GTAACGGGTACCAAAATTTACCTTAAACCCAACGAAAAAATCGCTGTAATTGACCTTGTTTACGGATTAATGTTGGAATC
GGGAAATGATGCGGCTAACGCCCTGGCGTGGGTTGTAGGAAAGGAAAATTTTATGTTAATATTAAATTATTATTTAAAAC
AAATGGGTTTAACCTCGGTTTCTTTTAAAAACCCCTCCGGCCTTTCTGCCCCGGGTCACAAAATTTCGGCGAAAGACCTA
TGCGTTTTGACCTTTTACGCATTATTAAAAGATGAATTTGCCGATATATGTAAAACAAAACAATATGAGGCGCAAAGTGG
CTTTAAGCCAAGGGTATTTCACAATACTAACCGCTTGTTAGCTGTCGATTCCCGGGTAATAGGGGTTAAAACCGGTACAA
CCAATGAAGCGGGTAAATGTTTAGTAGGGGCCTGGCGGGGAAACAGTTTCTTAATTGGTACCGTTTTAAATTGTCCCGAT
AGGTTTACAGCCGTGTATAAAACTTTTAAAACAACCGATGAAAAGCTAAGAGAAAAAGTTATCTGGTCCAAAGGCCAAAA
AATGTATTTTAAAAAAAATAACTGGTGGACTTTAACAAAAGACGTTAAAATATTTTCTGTGGGTAATGAACATAAGCTTG
TTAAAGTAGTTATTGAAGACAGTGATTCCTTTGTTCGGGCATTATTTTTTTTAAATGGTGCATTTTTGGCCGAGCAAAAT
TTACAAAAAGTAAGATGA

Upstream 100 bases:

>100_bases
AAAAAAAATAAAAGCTAAAGGTTACCGGATAGTTACCGTTGGGGAAATCATAGATTAATATTTTACGCTTATTTGGCAAC
AATACAATTGGTGATAATAG

Downstream 100 bases:

>100_bases
TGGAGGTTTAGTATGATCCATGTTACCACCTTACCTAACAAAATAACCGTTCTGGTAGAAGAGATTCCCTACATTCGTTC
CGCGGCCATTGGCTTGTGGT

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MKRYLIPLLLCLLTLPVKVHGEIKAKAYYFFIPTTNQIIEAKNQDFPLPPASTTKIATALTALDLYPRNFTLTVKPEAVR
VTGTKIYLKPNEKIAVIDLVYGLMLESGNDAANALAWVVGKENFMLILNYYLKQMGLTSVSFKNPSGLSAPGHKISAKDL
CVLTFYALLKDEFADICKTKQYEAQSGFKPRVFHNTNRLLAVDSRVIGVKTGTTNEAGKCLVGAWRGNSFLIGTVLNCPD
RFTAVYKTFKTTDEKLREKVIWSKGQKMYFKKNNWWTLTKDVKIFSVGNEHKLVKVVIEDSDSFVRALFFLNGAFLAEQN
LQKVR

Sequences:

>Translated_325_residues
MKRYLIPLLLCLLTLPVKVHGEIKAKAYYFFIPTTNQIIEAKNQDFPLPPASTTKIATALTALDLYPRNFTLTVKPEAVR
VTGTKIYLKPNEKIAVIDLVYGLMLESGNDAANALAWVVGKENFMLILNYYLKQMGLTSVSFKNPSGLSAPGHKISAKDL
CVLTFYALLKDEFADICKTKQYEAQSGFKPRVFHNTNRLLAVDSRVIGVKTGTTNEAGKCLVGAWRGNSFLIGTVLNCPD
RFTAVYKTFKTTDEKLREKVIWSKGQKMYFKKNNWWTLTKDVKIFSVGNEHKLVKVVIEDSDSFVRALFFLNGAFLAEQN
LQKVR
>Mature_325_residues
MKRYLIPLLLCLLTLPVKVHGEIKAKAYYFFIPTTNQIIEAKNQDFPLPPASTTKIATALTALDLYPRNFTLTVKPEAVR
VTGTKIYLKPNEKIAVIDLVYGLMLESGNDAANALAWVVGKENFMLILNYYLKQMGLTSVSFKNPSGLSAPGHKISAKDL
CVLTFYALLKDEFADICKTKQYEAQSGFKPRVFHNTNRLLAVDSRVIGVKTGTTNEAGKCLVGAWRGNSFLIGTVLNCPD
RFTAVYKTFKTTDEKLREKVIWSKGQKMYFKKNNWWTLTKDVKIFSVGNEHKLVKVVIEDSDSFVRALFFLNGAFLAEQN
LQKVR

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=236, Percent_Identity=35.1694915254237, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI87082022, Length=259, Percent_Identity=30.1158301158301, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786851, Length=294, Percent_Identity=28.2312925170068, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI87082054, Length=197, Percent_Identity=30.4568527918782, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 36611; Mature: 36611

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRYLIPLLLCLLTLPVKVHGEIKAKAYYFFIPTTNQIIEAKNQDFPLPPASTTKIATAL
CCCHHHHHHHHHHHCCEEEECEEEEEEEEEEECCCCHHHHCCCCCCCCCCCCCHHHHHHH
TALDLYPRNFTLTVKPEAVRVTGTKIYLKPNEKIAVIDLVYGLMLESGNDAANALAWVVG
HHHEECCCCEEEEECCCEEEEEEEEEEECCCCCEEHHEEHHHHHHCCCCCHHHEEEEEEC
KENFMLILNYYLKQMGLTSVSFKNPSGLSAPGHKISAKDLCVLTFYALLKDEFADICKTK
CCCEEEHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
QYEAQSGFKPRVFHNTNRLLAVDSRVIGVKTGTTNEAGKCLVGAWRGNSFLIGTVLNCPD
CCHHHCCCCCEEEECCCCEEEECCEEEEEECCCCCCCCCEEEEEECCCEEEEEEEECCCH
RFTAVYKTFKTTDEKLREKVIWSKGQKMYFKKNNWWTLTKDVKIFSVGNEHKLVKVVIED
HHHHHHHHHHCHHHHHHHHHHHCCCCEEEEECCCCEEEEECEEEEEECCCCEEEEEEEEC
SDSFVRALFFLNGAFLAEQNLQKVR
CCHHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MKRYLIPLLLCLLTLPVKVHGEIKAKAYYFFIPTTNQIIEAKNQDFPLPPASTTKIATAL
CCCHHHHHHHHHHHCCEEEECEEEEEEEEEEECCCCHHHHCCCCCCCCCCCCCHHHHHHH
TALDLYPRNFTLTVKPEAVRVTGTKIYLKPNEKIAVIDLVYGLMLESGNDAANALAWVVG
HHHEECCCCEEEEECCCEEEEEEEEEEECCCCCEEHHEEHHHHHHCCCCCHHHEEEEEEC
KENFMLILNYYLKQMGLTSVSFKNPSGLSAPGHKISAKDLCVLTFYALLKDEFADICKTK
CCCEEEHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
QYEAQSGFKPRVFHNTNRLLAVDSRVIGVKTGTTNEAGKCLVGAWRGNSFLIGTVLNCPD
CCHHHCCCCCEEEECCCCEEEECCEEEEEECCCCCCCCCEEEEEECCCEEEEEEEECCCH
RFTAVYKTFKTTDEKLREKVIWSKGQKMYFKKNNWWTLTKDVKIFSVGNEHKLVKVVIED
HHHHHHHHHHCHHHHHHHHHHHCCCCEEEEECCCCEEEEECEEEEEECCCCEEEEEEEEC
SDSFVRALFFLNGAFLAEQNLQKVR
CCHHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]