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The map label for this gene is gatA

Identifier: 78044168

GI number: 78044168

Start: 977010

End: 978467

Strand: Direct

Name: gatA

Synonym: CHY_1102

Alternate gene names: 78044168

Gene position: 977010-978467 (Clockwise)

Preceding gene: 78045230

Following gene: 78043368

Centisome position: 40.68

GC content: 48.97

Gene sequence:

>1458_bases
TTGGAACTATATTACTTAAGTGCAAAAGAAATAACAGAAAAAATAAAGGCTAAAGAGATATCGGCGGTAGAGGTGGCAAA
AGCTACATTTGACCGGATAGAAGCGGTAGAGCCCAAAATTCAAGCTTATGTGACGGTAACCCGGGAGCTGGGACTTAAAA
TGGCCCGGGAAGTCGATGAAAAAATTGCCCGGGGAGAAGACCCGGGGCCGCTGGCGGGGGTACCGGTGGCCATCAAAGAT
AACATGTCTACCGCCGGGATTAGAACTACTTGTTCGTCCAAAATCCTGGAAAACTACATACCGCCGTATGACGCCACTGT
GGTGGAAAAGTTAAAGGAAGCTGGGGCGGTGTTTACCGGTAAAACCAATTTGGACGAGTTTGCCATGGGTTCCTCTACCG
AAAATTCCCGCTTTTTCCCCACCAGAAATCCCTGGGACTTAGAACGGGTGCCCGGTGGTTCTTCCGGTGGGTCGGCGGCG
AGTGTGGCTGCTGGAGAAGCGGTAGTGGCTCTGGGTTCCGATACCGGGGGTTCCATCCGCCAGCCTGCTGCTTTTTGCGG
AATAGTGGGCTTGAAACCTACATACGGGGCGGTAAGCCGGTATGGTTTGGTGGCTTTTGCCAGCTCCTTGGATCAAATCG
GACCTTTTGCCCGGACGGTGGAAGATGCGGCCCTTTTATTAAATGTGATTGCCGGTCACGACCCGAAAGATTCAACCTCT
GCCGATATTGAATATCCTGATTACTTAAGCTTTTTAAATCAGGACATTAAGGGCCTGAAAATTGGGCTGCCGAAGGAATA
TTTTATTGACGGTATTGATGCTGGAGTGAAAAAGGCGATTGACGATGCGATAAAGGTTTTGGAAAGTTTAGGAGCAGTCT
TTGAAGAGGTATCGTTACCTCATACCAAATATTCTCTTCCGGTGTACTATCTGATTGCTCCGGCGGAAGCTTCTTCCAAC
CTAGCCCGGTATGACGGAGTCCGTTACGGCTACCGGGATTTTGAAGCCGAAGATGTGGTAGAAATGTTCTCCCGGACCCG
GGCGGAAGGTTTTGGGGCTGAGGTAAAAAGACGGATCATGCTTGGTACCTATGCTTTAAGTGCAGGTTATTATGATGCTT
ATTACCTAAAGGCGCTGAAAGTAAGGACCCTTATTAAAGAAGATTTTGACCGGGCTTTTACCAAAGTAGATCTCCTTTTA
ACCCCAACCACTCCAACCCCGGCGTTTAAGTTTGGAGAAAAAACGTCCGACCCGGTTTCGATGTATCTTTCGGACATCTT
CACCATGGCCGTAAACTTGGCCGGCCTTCCCGGGATTTCGGTTCCGGCTGGTTTTGACGGTCATCTGCCGGTATCGTTCC
AGTTAATTGGCAAGCCTTTTGATGAAGGAACCTTACTGAAAGTTGCCCATGCCTTTGAGCAAAATACCGAATTTCATAAG
GCACGGCCCAAGCTTTAG

Upstream 100 bases:

>100_bases
TTCATCAGCATTTAGATCCCGAAAAAGCCCTGGCCAATGCTCCGGAACGGGAAGAAAACTTCTTTAAGGTGCCGAAAATT
ATTTAAAGGAGGGTACGGTT

Downstream 100 bases:

>100_bases
GAGGTGTATTATGCGCGAATACGAAGCGGTTATTGGGTTAGAAGTTCATGTGGAGTTAAAAACCAATACTAAAATCTTTT
GCAATTGTTCTACCGAGTTT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MELYYLSAKEITEKIKAKEISAVEVAKATFDRIEAVEPKIQAYVTVTRELGLKMAREVDEKIARGEDPGPLAGVPVAIKD
NMSTAGIRTTCSSKILENYIPPYDATVVEKLKEAGAVFTGKTNLDEFAMGSSTENSRFFPTRNPWDLERVPGGSSGGSAA
SVAAGEAVVALGSDTGGSIRQPAAFCGIVGLKPTYGAVSRYGLVAFASSLDQIGPFARTVEDAALLLNVIAGHDPKDSTS
ADIEYPDYLSFLNQDIKGLKIGLPKEYFIDGIDAGVKKAIDDAIKVLESLGAVFEEVSLPHTKYSLPVYYLIAPAEASSN
LARYDGVRYGYRDFEAEDVVEMFSRTRAEGFGAEVKRRIMLGTYALSAGYYDAYYLKALKVRTLIKEDFDRAFTKVDLLL
TPTTPTPAFKFGEKTSDPVSMYLSDIFTMAVNLAGLPGISVPAGFDGHLPVSFQLIGKPFDEGTLLKVAHAFEQNTEFHK
ARPKL

Sequences:

>Translated_485_residues
MELYYLSAKEITEKIKAKEISAVEVAKATFDRIEAVEPKIQAYVTVTRELGLKMAREVDEKIARGEDPGPLAGVPVAIKD
NMSTAGIRTTCSSKILENYIPPYDATVVEKLKEAGAVFTGKTNLDEFAMGSSTENSRFFPTRNPWDLERVPGGSSGGSAA
SVAAGEAVVALGSDTGGSIRQPAAFCGIVGLKPTYGAVSRYGLVAFASSLDQIGPFARTVEDAALLLNVIAGHDPKDSTS
ADIEYPDYLSFLNQDIKGLKIGLPKEYFIDGIDAGVKKAIDDAIKVLESLGAVFEEVSLPHTKYSLPVYYLIAPAEASSN
LARYDGVRYGYRDFEAEDVVEMFSRTRAEGFGAEVKRRIMLGTYALSAGYYDAYYLKALKVRTLIKEDFDRAFTKVDLLL
TPTTPTPAFKFGEKTSDPVSMYLSDIFTMAVNLAGLPGISVPAGFDGHLPVSFQLIGKPFDEGTLLKVAHAFEQNTEFHK
ARPKL
>Mature_485_residues
MELYYLSAKEITEKIKAKEISAVEVAKATFDRIEAVEPKIQAYVTVTRELGLKMAREVDEKIARGEDPGPLAGVPVAIKD
NMSTAGIRTTCSSKILENYIPPYDATVVEKLKEAGAVFTGKTNLDEFAMGSSTENSRFFPTRNPWDLERVPGGSSGGSAA
SVAAGEAVVALGSDTGGSIRQPAAFCGIVGLKPTYGAVSRYGLVAFASSLDQIGPFARTVEDAALLLNVIAGHDPKDSTS
ADIEYPDYLSFLNQDIKGLKIGLPKEYFIDGIDAGVKKAIDDAIKVLESLGAVFEEVSLPHTKYSLPVYYLIAPAEASSN
LARYDGVRYGYRDFEAEDVVEMFSRTRAEGFGAEVKRRIMLGTYALSAGYYDAYYLKALKVRTLIKEDFDRAFTKVDLLL
TPTTPTPAFKFGEKTSDPVSMYLSDIFTMAVNLAGLPGISVPAGFDGHLPVSFQLIGKPFDEGTLLKVAHAFEQNTEFHK
ARPKL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=498, Percent_Identity=41.566265060241, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI195972892, Length=491, Percent_Identity=27.4949083503055, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI166795287, Length=169, Percent_Identity=36.6863905325444, Blast_Score=78, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=441, Percent_Identity=41.0430839002268, Blast_Score=301, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI17537465, Length=496, Percent_Identity=26.008064516129, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17556264, Length=289, Percent_Identity=31.4878892733564, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71990152, Length=318, Percent_Identity=31.4465408805031, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17538252, Length=192, Percent_Identity=31.7708333333333, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17556276, Length=324, Percent_Identity=30.2469135802469, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17556278, Length=324, Percent_Identity=30.2469135802469, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17538254, Length=230, Percent_Identity=26.5217391304348, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=418, Percent_Identity=38.9952153110048, Blast_Score=262, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6319685, Length=411, Percent_Identity=33.0900243309002, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6320448, Length=229, Percent_Identity=27.9475982532751, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24648113, Length=456, Percent_Identity=42.9824561403509, Blast_Score=340, Evalue=9e-94,
Organism=Drosophila melanogaster, GI24652985, Length=488, Percent_Identity=27.2540983606557, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI19922090, Length=488, Percent_Identity=27.2540983606557, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24652981, Length=488, Percent_Identity=27.2540983606557, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24652983, Length=488, Percent_Identity=27.2540983606557, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24644968, Length=509, Percent_Identity=26.9155206286837, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI161078093, Length=511, Percent_Identity=28.5714285714286, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI45550774, Length=498, Percent_Identity=28.1124497991968, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24648435, Length=498, Percent_Identity=28.1124497991968, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24648437, Length=498, Percent_Identity=28.1124497991968, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI21356731, Length=500, Percent_Identity=26.4, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24648441, Length=425, Percent_Identity=27.2941176470588, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648439, Length=425, Percent_Identity=27.2941176470588, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_CARHZ (Q3AD36)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359948.1
- ProteinModelPortal:   Q3AD36
- SMR:   Q3AD36
- STRING:   Q3AD36
- GeneID:   3727284
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_1102
- NMPDR:   fig|246194.3.peg.1149
- TIGR:   CHY_1102
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- ProtClustDB:   PRK00012
- BioCyc:   CHYD246194:CHY_1102-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52568; Mature: 52568

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 79-79 ACT_SITE 154-154 ACT_SITE 178-178

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELYYLSAKEITEKIKAKEISAVEVAKATFDRIEAVEPKIQAYVTVTRELGLKMAREVDE
CEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEHHHHHHHHHHHHHH
KIARGEDPGPLAGVPVAIKDNMSTAGIRTTCSSKILENYIPPYDATVVEKLKEAGAVFTG
HHHCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEC
KTNLDEFAMGSSTENSRFFPTRNPWDLERVPGGSSGGSAASVAAGEAVVALGSDTGGSIR
CCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCEEEEECCCCCCCCC
QPAAFCGIVGLKPTYGAVSRYGLVAFASSLDQIGPFARTVEDAALLLNVIAGHDPKDSTS
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ADIEYPDYLSFLNQDIKGLKIGLPKEYFIDGIDAGVKKAIDDAIKVLESLGAVFEEVSLP
CCCCCHHHHHHHHCCCCCEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HTKYSLPVYYLIAPAEASSNLARYDGVRYGYRDFEAEDVVEMFSRTRAEGFGAEVKRRIM
CCEECCEEEEEEECCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
LGTYALSAGYYDAYYLKALKVRTLIKEDFDRAFTKVDLLLTPTTPTPAFKFGEKTSDPVS
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCHHH
MYLSDIFTMAVNLAGLPGISVPAGFDGHLPVSFQLIGKPFDEGTLLKVAHAFEQNTEFHK
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCHHH
ARPKL
CCCCC
>Mature Secondary Structure
MELYYLSAKEITEKIKAKEISAVEVAKATFDRIEAVEPKIQAYVTVTRELGLKMAREVDE
CEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEHHHHHHHHHHHHHH
KIARGEDPGPLAGVPVAIKDNMSTAGIRTTCSSKILENYIPPYDATVVEKLKEAGAVFTG
HHHCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEC
KTNLDEFAMGSSTENSRFFPTRNPWDLERVPGGSSGGSAASVAAGEAVVALGSDTGGSIR
CCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCEEEEECCCCCCCCC
QPAAFCGIVGLKPTYGAVSRYGLVAFASSLDQIGPFARTVEDAALLLNVIAGHDPKDSTS
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ADIEYPDYLSFLNQDIKGLKIGLPKEYFIDGIDAGVKKAIDDAIKVLESLGAVFEEVSLP
CCCCCHHHHHHHHCCCCCEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HTKYSLPVYYLIAPAEASSNLARYDGVRYGYRDFEAEDVVEMFSRTRAEGFGAEVKRRIM
CCEECCEEEEEEECCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
LGTYALSAGYYDAYYLKALKVRTLIKEDFDRAFTKVDLLLTPTTPTPAFKFGEKTSDPVS
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCHHH
MYLSDIFTMAVNLAGLPGISVPAGFDGHLPVSFQLIGKPFDEGTLLKVAHAFEQNTEFHK
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCHHH
ARPKL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA