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The map label for this gene is leuC2 [H]

Identifier: 78043811

GI number: 78043811

Start: 981249

End: 982502

Strand: Direct

Name: leuC2 [H]

Synonym: CHY_1105

Alternate gene names: 78043811

Gene position: 981249-982502 (Clockwise)

Preceding gene: 78044757

Following gene: 78043571

Centisome position: 40.86

GC content: 48.48

Gene sequence:

>1254_bases
TTGGGAAAAACAATCATTGAAAAAATCCTTTCGCAAAAAAGCGGCGTTGATGCTTATGCGGGCCAAATTGTGGTGGCCGA
TGTGGATTACGTTATGGGGCAGGACGGAACGGCTCCCCTGGCAATAAAATCCTTTAAAGAAATGGGCGGGAAAAAAGTTT
TTGATCCGGAAAAAGTGGTTTTTGTGATAGACCACAGCGCTCCCAGCCCCAATGAAGGGGTTTCGCGGCTTCACAAATTG
ATGCGGGAGTTTCGGGACGAGCACGGAATTGATTTCCACGATATTGGCGATGGAGTCTGCCACCAGGTAGTGCCGGAAAG
CGGTAAAGTAGGTCCCGGCTCTCTGGTAGTAGGTGCCGATTCCCATACCTGCACTTACGGGGCGTTAAATGCCTTTGCTA
CCGGTGTAGGATCTACCGATCTGGCAGCGGCGATTATTTCCGGGAAGCTCTGGTTTAAAGTTCCCGAAACGATAAAATTT
GTGTTAAAGGGTAAACTTCCCAAAGGAGTATTTGCCAAGGATTTAATTTTGTATTTAATCGGGGATGTAACCGCCGATGG
AGCAACCTATATGGCCGCGGAATACACCGGGGAAGCTATTTCGGACCTTTCCATGGATGGTCGCTTTACCATTGCCAATA
TGGCTATTGAAATGGGAGCAAAAGCCGGATTGATGGAAGCCGATGAAAAAACTTTTGCTTTTGTAAAAGAACACGGCGGT
AAGGATTTTACACCGGTCTTTGCCGATCCGGATGCGGTTTATGCTAAAGTTTTGGAATATGACGTATCGGAGTTGGAACC
GATGGTGGCCAAGCCGCATACCGTTGATAACACCGCCAGAGTTACCGAGGTGGCCGGTACTCCGATTCACCAGGCGGTAC
TGGGAACCTGTACAAACGGACGGCTTGAAGATTTACGGATTGCAGCCAAAATCCTTGAGGGCAGAAAAATCCATCCCAGA
ACCCGCCTGATTGTGGCGCCGGCTTCCCGCAAAATTTTCCTAAAAGCCATGGAAGAAGGACTTATTAAAATTTTTGTGGA
AGCCGGGGCTGCCGTAGTTACACCGGGCTGTGGTCCTTGTGTGGGTACCCATAACGGTATACCTTCAGACGGAGAAAATG
TTATTTCAACTGCCAACCGGAATTTCAAAGGCCGGATGGGTAACAGTAATGCCTTTATCTATTTAGCTTCACCGGCCACA
GTTGCTGCTTCGGCTATTGCCGGTGAGATTGCGGACCCGAGACCTTATTTTTAA

Upstream 100 bases:

>100_bases
CCCATGTCCGGGCCCATGCGGTGGAATTAAAACGGTCTTTGTTTGATAAAGAGCTTGTTTTAATCTACGAAGAACATTTC
GGAAAGCGGGGAATGTAGCT

Downstream 100 bases:

>100_bases
GGATGGTGGAGTAAAAAATGATTTTAAGGGGTAAATGCCATAAATTTGGCAATGACGTCAATACCGATTATATAATTTCC
GGTAAATATAAGTTTAAAAC

Product: 3-isopropylmalate dehydratase large subunit family protein

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2 [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MGKTIIEKILSQKSGVDAYAGQIVVADVDYVMGQDGTAPLAIKSFKEMGGKKVFDPEKVVFVIDHSAPSPNEGVSRLHKL
MREFRDEHGIDFHDIGDGVCHQVVPESGKVGPGSLVVGADSHTCTYGALNAFATGVGSTDLAAAIISGKLWFKVPETIKF
VLKGKLPKGVFAKDLILYLIGDVTADGATYMAAEYTGEAISDLSMDGRFTIANMAIEMGAKAGLMEADEKTFAFVKEHGG
KDFTPVFADPDAVYAKVLEYDVSELEPMVAKPHTVDNTARVTEVAGTPIHQAVLGTCTNGRLEDLRIAAKILEGRKIHPR
TRLIVAPASRKIFLKAMEEGLIKIFVEAGAAVVTPGCGPCVGTHNGIPSDGENVISTANRNFKGRMGNSNAFIYLASPAT
VAASAIAGEIADPRPYF

Sequences:

>Translated_417_residues
MGKTIIEKILSQKSGVDAYAGQIVVADVDYVMGQDGTAPLAIKSFKEMGGKKVFDPEKVVFVIDHSAPSPNEGVSRLHKL
MREFRDEHGIDFHDIGDGVCHQVVPESGKVGPGSLVVGADSHTCTYGALNAFATGVGSTDLAAAIISGKLWFKVPETIKF
VLKGKLPKGVFAKDLILYLIGDVTADGATYMAAEYTGEAISDLSMDGRFTIANMAIEMGAKAGLMEADEKTFAFVKEHGG
KDFTPVFADPDAVYAKVLEYDVSELEPMVAKPHTVDNTARVTEVAGTPIHQAVLGTCTNGRLEDLRIAAKILEGRKIHPR
TRLIVAPASRKIFLKAMEEGLIKIFVEAGAAVVTPGCGPCVGTHNGIPSDGENVISTANRNFKGRMGNSNAFIYLASPAT
VAASAIAGEIADPRPYF
>Mature_416_residues
GKTIIEKILSQKSGVDAYAGQIVVADVDYVMGQDGTAPLAIKSFKEMGGKKVFDPEKVVFVIDHSAPSPNEGVSRLHKLM
REFRDEHGIDFHDIGDGVCHQVVPESGKVGPGSLVVGADSHTCTYGALNAFATGVGSTDLAAAIISGKLWFKVPETIKFV
LKGKLPKGVFAKDLILYLIGDVTADGATYMAAEYTGEAISDLSMDGRFTIANMAIEMGAKAGLMEADEKTFAFVKEHGGK
DFTPVFADPDAVYAKVLEYDVSELEPMVAKPHTVDNTARVTEVAGTPIHQAVLGTCTNGRLEDLRIAAKILEGRKIHPRT
RLIVAPASRKIFLKAMEEGLIKIFVEAGAAVVTPGCGPCVGTHNGIPSDGENVISTANRNFKGRMGNSNAFIYLASPATV
AASAIAGEIADPRPYF

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=416, Percent_Identity=29.8076923076923, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI8659555, Length=491, Percent_Identity=25.050916496945, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1786259, Length=470, Percent_Identity=33.8297872340426, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI87081781, Length=350, Percent_Identity=26.5714285714286, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI2367097, Length=455, Percent_Identity=25.4945054945055, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1787531, Length=389, Percent_Identity=25.706940874036, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI25149337, Length=416, Percent_Identity=29.5673076923077, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI32564738, Length=411, Percent_Identity=29.4403892944039, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI25149342, Length=284, Percent_Identity=29.5774647887324, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17568399, Length=398, Percent_Identity=24.8743718592965, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320440, Length=447, Percent_Identity=35.5704697986577, Blast_Score=230, Evalue=4e-61,
Organism=Saccharomyces cerevisiae, GI6321429, Length=451, Percent_Identity=31.2638580931264, Blast_Score=178, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6323335, Length=417, Percent_Identity=32.6139088729017, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6322261, Length=419, Percent_Identity=30.3102625298329, Blast_Score=171, Evalue=1e-43,
Organism=Drosophila melanogaster, GI281365315, Length=448, Percent_Identity=29.0178571428571, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI17864292, Length=448, Percent_Identity=29.0178571428571, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI161076999, Length=411, Percent_Identity=28.9537712895377, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI28571643, Length=416, Percent_Identity=28.8461538461538, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI17137564, Length=500, Percent_Identity=25.6, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24645686, Length=406, Percent_Identity=25.3694581280788, Blast_Score=89, Evalue=8e-18,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 44272; Mature: 44141

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTIIEKILSQKSGVDAYAGQIVVADVDYVMGQDGTAPLAIKSFKEMGGKKVFDPEKVV
CCHHHHHHHHHHCCCCCHHHCEEEEEECHHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEE
FVIDHSAPSPNEGVSRLHKLMREFRDEHGIDFHDIGDGVCHQVVPESGKVGPGSLVVGAD
EEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHCCCCCCCCCCCEEEECC
SHTCTYGALNAFATGVGSTDLAAAIISGKLWFKVPETIKFVLKGKLPKGVFAKDLILYLI
CCCEEHHHHHHHHHCCCCHHHHHHHHCCEEEEECHHHHHHHHHCCCCCCHHHHHHHHHHH
GDVTADGATYMAAEYTGEAISDLSMDGRFTIANMAIEMGAKAGLMEADEKTFAFVKEHGG
HCCCCCCCEEEEEHHCCHHHHHCCCCCCEEEEHHHHHHCCCCCCCCCCCHHHHHHHHCCC
KDFTPVFADPDAVYAKVLEYDVSELEPMVAKPHTVDNTARVTEVAGTPIHQAVLGTCTNG
CCCCCEECCCHHHHHHHHHHCHHHCCHHHCCCCCCCCCHHEEECCCCCHHHHHHHCCCCC
RLEDLRIAAKILEGRKIHPRTRLIVAPASRKIFLKAMEEGLIKIFVEAGAAVVTPGCGPC
CHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHCCHHEEHHHCCCEEECCCCCCC
VGTHNGIPSDGENVISTANRNFKGRMGNSNAFIYLASPATVAASAIAGEIADPRPYF
CCCCCCCCCCCCHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
GKTIIEKILSQKSGVDAYAGQIVVADVDYVMGQDGTAPLAIKSFKEMGGKKVFDPEKVV
CHHHHHHHHHHCCCCCHHHCEEEEEECHHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEE
FVIDHSAPSPNEGVSRLHKLMREFRDEHGIDFHDIGDGVCHQVVPESGKVGPGSLVVGAD
EEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHCCCCCCCCCCCEEEECC
SHTCTYGALNAFATGVGSTDLAAAIISGKLWFKVPETIKFVLKGKLPKGVFAKDLILYLI
CCCEEHHHHHHHHHCCCCHHHHHHHHCCEEEEECHHHHHHHHHCCCCCCHHHHHHHHHHH
GDVTADGATYMAAEYTGEAISDLSMDGRFTIANMAIEMGAKAGLMEADEKTFAFVKEHGG
HCCCCCCCEEEEEHHCCHHHHHCCCCCCEEEEHHHHHHCCCCCCCCCCCHHHHHHHHCCC
KDFTPVFADPDAVYAKVLEYDVSELEPMVAKPHTVDNTARVTEVAGTPIHQAVLGTCTNG
CCCCCEECCCHHHHHHHHHHCHHHCCHHHCCCCCCCCCHHEEECCCCCHHHHHHHCCCCC
RLEDLRIAAKILEGRKIHPRTRLIVAPASRKIFLKAMEEGLIKIFVEAGAAVVTPGCGPC
CHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHCCHHEEHHHCCCEEECCCCCCC
VGTHNGIPSDGENVISTANRNFKGRMGNSNAFIYLASPATVAASAIAGEIADPRPYF
CCCCCCCCCCCCHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]