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The map label for this gene is leuD2 [H]

Identifier: 78043571

GI number: 78043571

Start: 982520

End: 983017

Strand: Direct

Name: leuD2 [H]

Synonym: CHY_1106

Alternate gene names: 78043571

Gene position: 982520-983017 (Clockwise)

Preceding gene: 78043811

Following gene: 78042663

Centisome position: 40.91

GC content: 39.96

Gene sequence:

>498_bases
ATGATTTTAAGGGGTAAATGCCATAAATTTGGCAATGACGTCAATACCGATTATATAATTTCCGGTAAATATAAGTTTAA
AACACTGGATATGAACGAGCTGGCCAGACACGTGATGGAAGATTTAGACCCGGATTTTTACAACAAAATAACCCCCGGTG
ATTTTATCGTTGCCGGCAGTAACTTTGGCTGCGGTTCATCCCGGGAACAAGCACCGCTGGCCATTAAATACGCTAAAATT
GGTGCTGTGATTGCTAAGTCCTTTGCCCGGATTTTTTACCGAAATGCAATTAATACCGGTCTTCCGGTAATTGAGTGTGA
TACCGATAAAATAAATGCCGGCGATGAACTGGAAGTAGATTTGTCTAAAGGGATTATTAGAAACCTGACTACCGGCGAGG
ATATACCGATTAAGCCATTGCCGGAGGTAATGATCAAGATTTTAAATGACGGCGGGTTAATTGAACATTTTAAAAAATAC
GGCACATTTAACTTTTAA

Upstream 100 bases:

>100_bases
CCTTTATCTATTTAGCTTCACCGGCCACAGTTGCTGCTTCGGCTATTGCCGGTGAGATTGCGGACCCGAGACCTTATTTT
TAAGGATGGTGGAGTAAAAA

Downstream 100 bases:

>100_bases
GGAGGTTCATGATGGCGCATAGAGTTACATTGATTCCCGGAGACGGAATTGGTCCAGAAATTGTTGAAGCAGCCCGGCGG
GTAATTGATGCCAGCGGAGC

Product: 3-isopropylmalate dehydratase small subunit family protein

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2 [H]

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MILRGKCHKFGNDVNTDYIISGKYKFKTLDMNELARHVMEDLDPDFYNKITPGDFIVAGSNFGCGSSREQAPLAIKYAKI
GAVIAKSFARIFYRNAINTGLPVIECDTDKINAGDELEVDLSKGIIRNLTTGEDIPIKPLPEVMIKILNDGGLIEHFKKY
GTFNF

Sequences:

>Translated_165_residues
MILRGKCHKFGNDVNTDYIISGKYKFKTLDMNELARHVMEDLDPDFYNKITPGDFIVAGSNFGCGSSREQAPLAIKYAKI
GAVIAKSFARIFYRNAINTGLPVIECDTDKINAGDELEVDLSKGIIRNLTTGEDIPIKPLPEVMIKILNDGGLIEHFKKY
GTFNF
>Mature_165_residues
MILRGKCHKFGNDVNTDYIISGKYKFKTLDMNELARHVMEDLDPDFYNKITPGDFIVAGSNFGCGSSREQAPLAIKYAKI
GAVIAKSFARIFYRNAINTGLPVIECDTDKINAGDELEVDLSKGIIRNLTTGEDIPIKPLPEVMIKILNDGGLIEHFKKY
GTFNF

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6320440, Length=96, Percent_Identity=42.7083333333333, Blast_Score=83, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321429, Length=132, Percent_Identity=35.6060606060606, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 18395; Mature: 18395

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILRGKCHKFGNDVNTDYIISGKYKFKTLDMNELARHVMEDLDPDFYNKITPGDFIVAGS
CCCCCCCHHCCCCCCCCEEEECCEEEEEECHHHHHHHHHHHCCHHHHCCCCCCCEEEECC
NFGCGSSREQAPLAIKYAKIGAVIAKSFARIFYRNAINTGLPVIECDTDKINAGDELEVD
CCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEE
LSKGIIRNLTTGEDIPIKPLPEVMIKILNDGGLIEHFKKYGTFNF
HHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MILRGKCHKFGNDVNTDYIISGKYKFKTLDMNELARHVMEDLDPDFYNKITPGDFIVAGS
CCCCCCCHHCCCCCCCCEEEECCEEEEEECHHHHHHHHHHHCCHHHHCCCCCCCEEEECC
NFGCGSSREQAPLAIKYAKIGAVIAKSFARIFYRNAINTGLPVIECDTDKINAGDELEVD
CCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEE
LSKGIIRNLTTGEDIPIKPLPEVMIKILNDGGLIEHFKKYGTFNF
HHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]